DIFFERENCES IN MOLECULAR WEIGHT BETWEEN CORRESPONDING ANIONIC PEROXIDASE ISOZYMES FROM TWO FLAX GENOTROPHS

1980 ◽  
Vol 22 (4) ◽  
pp. 529-534 ◽  
Author(s):  
H. Tyson ◽  
M. A. Fieldes
Keyword(s):  
Molecular Weight ◽  
Linear Regression ◽  
Linum Usitatissimum ◽  
Gel Electrophoresis ◽  
Relative Mobility ◽  
Anionic Peroxidase ◽  
Linum Usitatissimum L ◽  
Peroxidase Isozymes ◽  
Flax Genotrophs ◽  
Adult Plants

Anionic peroxidase isozymes from main stem tissues of adult plants of two flax (Linum usitatissimum L.) genotrophs were separated using acrylamide gel electrophoresis. A range of seven acrylamide concentrations was used for the gels, enabling the effect of gel concentration on relative mobility (Rm) to be examined. The regression of log (Rm) on gel concentration was linear for two of the four main isozymes found. Differences in linear regression slope between the L and S flax genotroph isozymes suggested genotroph differences in molecular weight.

ACTIVITY AND RELATIVE MOBILITY OF PEROXIDASE ISOENZYMES IN GENOTROPHS AND GENOTYPES OF FLAX (LINUM USITATISSIMUM L.)

1972 ◽  
Vol 14 (3) ◽  
pp. 625-636 ◽  
Author(s):  
M. A. Fieldes ◽  
H. Tyson
Keyword(s):  
Linum Usitatissimum ◽  
Gel Electrophoresis ◽  
Relative Mobility ◽  
Peroxidase Isoenzymes ◽  
Anionic Peroxidase ◽  
Linum Usitatissimum L ◽  
Phosphorus And Potassium ◽  
Highly Correlated ◽  
Successive Generations ◽  
Nitrogen Phosphorus

Acrylamide gel electrophoresis was used to separate anionic peroxidase isoenzymes in genotypes and genotrophs of flax. Activities and relative mobilities were measured directly from the separations on the gels.The effects of growth of one flax genotype in soil supplemented by either nitrogen, phosphorus and potassium (NPK) or nitrogen and potassium (NK) on subsequent generations of its progeny produced by complete selfing were studied. Both activity and relative mobility of anionic peroxidase isoenzymes displayed effects of fertilizer treatments applied in previous generations. NPK increased the relative mobility of all isoenzymes, while depressing the activity of at least three of them. Successive generations of growth in NPK produced approximately linear increases in relative mobility. Such environmentally induced heritable changes were detectable five generations later.Two other flax genotypes were crossed, and relative mobility and activity of anionic peroxidase isoenzymes were examined in both parents and F2 progeny. Between parents, there were differences in relative mobility for two of the four isoenzymes; their F2 hybrids showed intermediate mobility for these particular isoenzymes. There were no differences between reciprocal F2 hybrids for mobility or activity of any isoenzyme. The parents differed in activity in all four isoenzymes; the F2 hybrids displayed dominance towards the lower activity parent for each of the isoenzymes.Total anionic isoenzyme activity was highly correlated with gross peroxidase activity measured prior to electrophoretic separation.

PROTEIN PROFILES OF FLAX GENOTROPHS

1982 ◽  
Vol 24 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Mary Ann Fieldes ◽  
Hugh Tyson ◽  
David Marriott
Keyword(s):  
Linum Usitatissimum ◽  
Plant Proteins ◽  
Two Dimensional ◽  
Stem Tissue ◽  
Protein Profiles ◽  
Relative Mobility ◽  
Two Dimensional Electrophoresis ◽  
Linum Usitatissimum L ◽  
Flax Genotrophs ◽  
Dimensional Electrophoresis

Protein profiles of partially purified protein extracts from main stem tissue of Durrant's L and S flax (Linum usitatissimum L.) genotrophs were examined with one and two dimensional electrophoresis on acrylamide gels. The purification retained mainly glycoproteins. Among this reduced spectrum of plant proteins, some of the proteins separated had relative mobility (Rm) shifts between L and S. For two proteins, the Rm shifts were demonstrated in two-dimensional separations using mixtures of the L and S extracts. The Rm shifts were all in the same direction, the S protein ran slightly slower than the corresponding L protein, in both dimensions. This shift direction agreed with previous studies on Rm shifts with peroxidase and esterase isozymes and with similar shifts in acid phosphatase isozymes.

scholarly journals Preliminary characterization of peroxidase isozymes isolated from two flax genotrophs

1977 ◽  
Vol 55 (11) ◽  
pp. 1465-1473 ◽  
Author(s):  
M. A. Fieldes ◽  
C. L. Deal ◽  
H. Tyson
Keyword(s):  
Molecular Weight ◽  
Oxidase Activity ◽  
Indoleacetic Acid ◽  
Relative Mobility ◽  
Fresh Weight ◽  
Iaa Oxidase ◽  
Peroxidase Isozymes ◽  
Flax Genotrophs ◽  
Very High

Four peroxidase (EC 1.11.1.7) isozymes were isolated from each of two flax genotrophs. All four isozymes were glycoproteins and all exhibited indoleacetic acid (IAA) oxidase activity. The percentage purity of two of the isozymes was very high; these isozymes differed in percentage carbohydrate and in peroxidase and IAA oxidase specific activities. Three of the isozymes displayed molecular weight values of about 43 000; for the fourth, molecular weight was considerably higher. Corresponding isozymes from the genotrophs and from two other flax genotypes displayed molecular weight differences which corresponded to electrophoretic relative mobility differences. Enzyme yield per unit fresh weight was higher for one genotroph than the other, and the balance between peroxidase activity and IAA oxidase activity between the genotrophs was different.

ACTIVITY AND RELATIVE MOBILITY OF PEROXIDASE AND ESTERASE ISOZYMES OF FLAX (LINUM USITATISSIMUM) GENOTROPHS. I. DEVELOPING MAIN STEMS

1973 ◽  
Vol 15 (4) ◽  
pp. 731-744 ◽  
Author(s):  
M. A. Fieldes ◽  
H. Tyson
Keyword(s):  
Linum Usitatissimum ◽  
Relative Mobility ◽  
Esterase Isozymes ◽  
Anionic Peroxidase ◽  
Rates Of Change ◽  
Peroxidase Isozymes

The effects of growth of one flax genotype in soil supplemented by either nitrogen, phosphorous and potassium (NPK) or nitrogen and potassium (NK) on its progeny produced by several generations of complete selfing were studied. The two types of progeny produced, L induced by NPK and S induced by NK, were examined at 4-day intervals from the 16th to the 36th day after sowing. Both activity and relative mobility of the anionic peroxidase and esterase isozymes displayed effects of the fertilizer treatments applied in the treatment generation. NPK, compared to NK, increased the relative mobility of all the peroxidase and esterase isozymes, while depressing the activities of the peroxidase isozymes and increasing those of the esterase isozymes, at all ages examined. Some differences in the rates of change, with age, of the characteristics of L and S were distinguished; the differences observed between L and S could not, however, be attributed to differences in their developmental ages.

COMPARATIVE THERMAL STABILITY OF PEROXIDASE ISOZYMES FROM TWO FLAX GENOTROPHS

1982 ◽  
Vol 24 (4) ◽  
pp. 427-435 ◽  
Author(s):  
Mary Ann Fieldes ◽  
Hugh Tyson
Keyword(s):  
Thermal Stability ◽  
Molecular Weight ◽  
Prolonged Treatment ◽  
Linum Usitatissimum L ◽  
Peroxidase Isozymes ◽  
Heat Treated ◽  
Peak Areas ◽  
Flax Genotrophs ◽  
Thermal Stability Of

The thermal stability of peroxidase isozymes was examined in vitro in Linum usitatissimum L. Extracts of main stem tissues of the L and S genotrophs produced by Durrant were heat treated over a range of temperatures and times. Isozymes in treated extracts were separated electrophoretically, and peak areas for the four main anionic isozymes, together with their relative mobilities (Rms), were recorded. Peak areas supplied estimates of relative activities. Short duration treatments at 60° and 70 °C demonstrated differences in thermal stability between isozymes and produced changes in Rm. With prolonged treatment at 40 °C, the thermal stability of one isozyme differed from those of the other three. This isozyme was known to have a higher molecular weight than the others. In addition, prolonged treatment at 40 °C demonstrated increased thermal stability of the three lower molecular weight isozymes of genotroph S compared to those of L.

scholarly journals Isolation of peroxidase isozymes from two flax genotypes by column chromatography

1976 ◽  
Vol 54 (11) ◽  
pp. 1180-1188 ◽  
Author(s):  
M. A. Fieldes ◽  
N. Bashour ◽  
C. L. Deal ◽  
H. Tyson
Keyword(s):  
Molecular Weight ◽  
Horseradish Peroxidase ◽  
Column Chromatography ◽  
Gel Electrophoresis ◽  
Apparent Molecular Weight ◽  
Relative Mobility ◽  
Peroxidase Isozymes ◽  
Chromatographic Elution

Isolation of the four major peroxidase isozymes (isozymes 1, 2, 3, and 4) of two flax genotypes was achieved by modifying the procedure used by Shannon et al. (1966) for the isolation of horseradish peroxidase isozymes. The net positive and net negative charges of isozymes 1, 2, and 4 were different. Isozyme 3 resembled isozyme 4 in charge but differed in apparent molecular weight. The chromatographic elution profiles of both genotypes were the same. Anionic gel electrophoresis demonstrated that after isolation and repurification, relative mobility differences existed between the corresponding isozymes of the two genotypes for all four isozymes.

Molecular weight and net charge of peroxidase isozymes in F1 hybrids between L and S flax genotrophs

1982 ◽  
Vol 20 (9-10) ◽  
pp. 919-927 ◽  
Author(s):  
Hugh Tyson ◽  
Mary Ann Fieldes
Keyword(s):  
Molecular Weight ◽  
F1 Hybrids ◽  
Net Charge ◽  
Peroxidase Isozymes ◽  
Flax Genotrophs

Peroxidase activity and relative mobility at anthesis in flax genotrophs and their F2 progeny: developmental and genetic effects

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 495-504 ◽  
Author(s):  
M. A. Fieldes ◽  
J. Ross
Keyword(s):  
Genetic Control ◽  
Peroxidase Activity ◽  
Stem Elongation ◽  
Genetic Regulation ◽  
Relative Mobility ◽  
Activity Data ◽  
Peroxidase Isozymes ◽  
Developmental Age ◽  
Total Peroxidase Activity ◽  
Flax Genotrophs

The genetic regulation of the environmentally induced heritable difference in peroxidase activity between Durrant's large (L) and small (S) flax genotrophs was examined in leaves from plants ranging in developmental age from 6 days before anthesis to 3 days after. Mean peroxidase activity was higher for S than L and intermediate for the reciprocal F2's from L × S and S × L crosses (F2L × S and F2S × L). However, activity increased with development and, since there were small but significant differences in the average developmental ages of L, S, F2L × S, and F2S × L plants, the effects of development on activity had to be taken into account in examining the F2 activity data for segregation. A regression method was used to remove developmental effects and, underlying these effects, total peroxidase activity appeared to be regulated by a single locus with two alleles and L dominance. Two other dimorphic loci, both described previously, were also examined. One regulates the presence-absence of septa hairs in the seed capsules and the other the relative mobility of anionic peroxidase isozymes. There was no phenotypic linkage between the three segregating parameters. The genetic control of activity appeared to regulate cationic rather than anionic activity. In addition, a relationship between activity and plant height indicated either that peroxidase activity is one of the factors regulating main stem elongation or that the locus regulating peroxidase activity is linked to one of the loci involved in the regulation of plant height.Key words: flax genotrophs, peroxidase, genetic control, development.

scholarly journals Anomalous migration of leghaemoglobin on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis

1978 ◽  
Vol 169 (1) ◽  
pp. 251-253 ◽  
Author(s):  
P Lehtovaara
Keyword(s):  
Molecular Weight ◽  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Relative Mobility ◽  
Concomitant Decrease ◽  
Helical Content

The estimate of the molecular weight of leghaemoglobin by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis is about 20% too low. This is due to an anomalously high limiting relative mobility. Leghaemoglobin binds 1.4 g of sodium dodecyl sulphate/g of protein with a concomitant decrease in the helical content from 71-72% to 49-51%.

SDS-Polyacrylamide Electrophoresis Pattern Alteration of Myofibrillar Proteins after Heating of Avian Leg and Breast Muscle1

1977 ◽  
Vol 40 (4) ◽  
pp. 261-264 ◽  
Author(s):  
F. LEON CRESPO ◽  
H. W. OCKERMAN
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Myofibrillar Proteins ◽  
Relative Mobility ◽  
Polyacrylamide Electrophoresis ◽  
Avian Muscle ◽  
Reduced Intensity ◽  
Electrophoresis Pattern

The SDS-polyacrylamide disc gel electrophoresis patterns of myofibrillar proteins from leg and breast avian muscle heated to 45, 50, 55, and 60 C were evaluated. Most of the detected bands were tentatively identified on the basis of molecular weight calculated from their Rm (relative mobility). Heating the tissue caused denaturation of protein which resulted in reduced intensity and ultimate disappearance of the bands as temperature increased. Bands identified as myosin were quite sensitive to heat compared to the greater resistance of the G-actin band.

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