Genome size and base composition in Medicago sativa and M. truncatula species

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 264-270 ◽  
Author(s):  
François Blondon ◽  
Dominique Marie ◽  
Spencer Brown ◽  
Adam Kondorosi
Keyword(s):  
Flow Cytometry ◽  
Medicago Sativa ◽  
Key Words ◽  
Genome Size ◽  
Medicago Truncatula ◽  
Intraspecific Variation ◽  
Base Composition ◽  
Annual Medicago

The genome size (1C value) and base composition of 14 ecotypes of two species of tetraploid and diploid Medicago have been assessed by flow cytometry. These parameters vary both between and within species. The diploid annual Medicago truncatula Gaertn. had the smallest genome of the group studied (which also covered M. sativa L. subsp. sativa, M. sativa L. subsp. caerulea (Less. ex Ledeb.) Schmalh., M. sativa L. subsp. quasifalcata Sinsk., M. sativa L. subsp. × varia (Martyn) Arcangeli; however, its ecotypes revealed substantial intraspecific variation. The smallest M. truncatula genome observed was ecotype 108-1 with 1C = 0.49 pg and 38.1% GC and the largest was Jemalong with 1C = 0.57 pg and 38.6% GC. The degree of polysomaty in these Medicago was low, although in some tissues the frequency of cells with 4C nuclei reached 50%.Key words: Medicago, genome size, base composition, flow cytometry, symbiosis.

Larger genomes for molluskan land pioneers

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 211-212 ◽  
Author(s):  
Alexander E Vinogradov
Keyword(s):  
Flow Cytometry ◽  
Key Words ◽  
Genome Evolution ◽  
Genome Size ◽  
Base Composition ◽  
Noncoding Dna

The terrestrial pulmonate mollusks were found to have the significantly larger genomes than the aquatic pulmonates. Being shown in the independent phylogenetic branch, this phenomenon suggests that the previously observed genome enlargement in the vertebrate land pioneers (amphibians and lungfishes) was not casual. As in the vertebrates, the larger molluskan genomes are also more GC-rich. Key words: genome size, genome evolution, cytoecology, noncoding DNA, genome base composition, flow cytometry.

scholarly journals Flow cytometry as tool in plant sciences, with emphasis on genome size and ploidy level assessment

2018 ◽  
Vol 2 (2) ◽  
pp. 1 ◽  
Author(s):  
Mickael Bourge ◽  
Spencer Creig Brown ◽  
Sonja Siljak-Yakovlev
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Ploidy Level ◽  
Cell Biology ◽  
Base Composition ◽  
Good Practice ◽  
Plant Cells ◽  
Size Measurement ◽  
Fast Acquisition ◽  
Plant Sciences

Flow cytometry has become the method of choice to measure the DNA content (genome size) in plants. Ease of sample preparation, fast acquisition, and accurate measurements have made the method popular in the domains of plant cell biology, systematics, evolution, genetics and biotechnology. Although the cell wall is a problem when isolating plant cells, cytometry remains a powerful tool in plant sciences. Based on our 30-years’ experience in this field, this review will focus at first on genome size measurement using simply isolated nuclei: the good practice for acquisition, nuclei isolation, appropriate buffers, kind of tissues to use. The second part will briefly review what kind of measurements it is possible to make in plant cytometry, and for what purpose: base composition, ploidy level, cell cycle, endoreplication, seed screening, and nuclei/chromosomes sorting. We will address troubleshooting. The commonly-used mathematic tools will be discussed.

Genome size variation in Hordeum spontaneum populations

Genome ◽  
1999 ◽  
Vol 42 (6) ◽  
pp. 1094-1099 ◽  
Author(s):  
Timo Turpeinen ◽  
Johanna Kulmala ◽  
Eviatar Nevo
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Intraspecific Variation ◽  
Wild Barley ◽  
Size Variation ◽  
Hordeum Spontaneum ◽  
Genome Size Variation ◽  
Propidium Iodide Staining ◽  
Significant Difference ◽  
Positive Correlations

Populations of wild barley, Hordeum spontaneum (C. Koch), originating from 10 ecologically and geographically different sites in Israel, were assessed for genome size. Measurements were obtained by flow cytometry using propidium iodide staining. Genome sizes ranged from 9.35 to 9.81 pg. Variance analysis indicated a significant difference between populations. Genome sizes were positively correlated with mean January temperature. Our results corroborate previous findings of intraspecific variation in genome size from different plant species. The positive correlations between climate and genome size suggest that the latter is adaptive and determined by natural selection.Key words: Hordeum spontaneum, genome size, flow cytometry, intraspecific variation, natural selection.

scholarly journals Genome size and base composition of Bromeliaceae species assessed by flow cytometry

2012 ◽  
Vol 298 (6) ◽  
pp. 1185-1193 ◽  
Author(s):  
Fernanda Campanharo Favoreto ◽  
Carlos Roberto Carvalho ◽  
Andreia Barcelos Passos Lima ◽  
Adésio Ferreira ◽  
Wellington Ronildo Clarindo
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Base Composition

First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

2014 ◽  
Vol 92 (10) ◽  
pp. 847-851 ◽  
Author(s):  
Kelly L. Mulligan ◽  
Terra C. Hiebert ◽  
Nicholas W. Jeffery ◽  
T. Ryan Gregory
Keyword(s):  
Flow Cytometry ◽  
Image Analysis ◽  
Body Size ◽  
Genome Size ◽  
Positive Relationship ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Size Estimation ◽  
Size Diversity ◽  
Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

Comparative genome size estimation of different life stages of grey mullet, Mugil cephalus Linnaeus, 1758 by flow cytometry

2021 ◽  
Author(s):  
Jani Angel J. Raymond ◽  
Mudagandur Shashi Shekhar ◽  
Vinaya Kumar Katneni ◽  
Ashok Kumar Jangham ◽  
Sudheesh Kommu Prabhudas ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Mugil Cephalus ◽  
Life Stages ◽  
Size Estimation ◽  
Grey Mullet ◽  
Comparative Genome

scholarly journals Coconut genome size determined by flow cytometry: Tall versus Dwarf types

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
M. Freitas Neto ◽  
T.N.S. Pereira ◽  
I.G.C. Geronimo ◽  
A.O.N. Azevedo ◽  
S.R.R. Ramos ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Genome Size

Shearing force of alfalfa stems: Effects of genotype and year interactions

1998 ◽  
Vol 78 (4) ◽  
pp. 719-722
Author(s):  
A. D. Iwaasa ◽  
K. A. Beauchemin ◽  
S. N. Acharya ◽  
J. G. Buchanan-Smith
Keyword(s):  
Cell Wall ◽  
Medicago Sativa ◽  
Key Words ◽  
Chemical Constituents ◽  
Stem Segment ◽  
Shearing Force ◽  
Medicago Sativa L ◽  
Genotype Clustering ◽  
Force Characteristics

Shearing force of alfalfa (Medicago sativa L.) stems was measured to evaluate genotype-by-year interactions. Based on mean shearing force for each stem segment and genotype clustering criteria, several genotypes had similarly shearing forces among years. Therefore, selecting genotypes with desirable shearing force characteristics to improve digestibility or intake potential of forages may be possible. Key words: Genotype, environment, shearing force, cell wall chemical constituents

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