Zebrafish chromosome banding

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 1052-1055 ◽  
Author(s):  
L. P. Pijnacker ◽  
M. A. Ferwerda
Keyword(s):  
Danio Rerio ◽  
Sex Chromosomes ◽  
Chromosome Banding ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Zebrafish Chromosome ◽  
Danio Rerio Embryos

Banding techniques were carried out on metaphase chromosomes of zebrafish (Danio rerio) embryos. The karyotypes with the longest chromosomes consist of 12 metacentrics, 26 submetacentrics, and 12 subtelocentrics (2n = 50). All centromeres are C-band positive. Eight chromosomes have a pericentric C-band in each arm and 22 chromosomes have one in the longest arm. Two chromosomes have a slightly heterochromatic long arm and five chromosomes have an Ag-NOR at the terminal end of the long arm. Other banding patterns and sex chromosomes could not be revealed.Key words: zebrafish, karyotype, chromosome banding.

Taxonomic status of the black fly Prosimulium italicum Rivosecchi (Diptera: Simuliidae) based on genetic evidence

Zootaxa ◽  
2018 ◽  
Vol 4377 (2) ◽  
pp. 280 ◽  
Author(s):  
MATÚŠ KÚDELA ◽  
PETER H. ADLER ◽  
TATIANA KÚDELOVÁ
Keyword(s):  
Mitochondrial Dna ◽  
Central Europe ◽  
Sex Chromosomes ◽  
Chromosome Banding ◽  
Taxonomic Status ◽  
Northern Europe ◽  
Chromosome Polymorphism ◽  
Species Status ◽  
Banding Patterns ◽  
Black Fly

The black fly Prosimulium italicum Rivosecchi, distributed in the Apennines and Sicily, was described as a subspecies of Prosimulium hirtipes (Fries), based on a few morphological details. It subsequently was considered conspecific with P. hirtipes and the name was synonymized. Analyses of polytene chromosome banding patterns and sequences of mitochondrial DNA (COI and COII) revealed deep genetic divergence between P. italicum from Italy and P. hirtipes from northern and central Europe and confirmed the species status of P. italicum. Populations of P. italicum either lack chromosomal inversion IS-9 or carry it as an X-chromosome polymorphism, whereas all analyzed populations of P. hirtipes (Slovakia, Sweden, England, and Scotland) are fixed for IS-9. The average K2P genetic distance was 3.7% between P. italicum and P. hirtipes from northern Europe (Sweden) and 4.3 % between P. italicum and P. hirtipes from central Europe (Slovakia). Cytogenetic analysis showed the presence of two cytoforms of P. hirtipes (‘A’ in Sweden and Slovakia and ‘B’ in England and Scotland) and two cytoforms of P. italicum (‘A’ in Sicily and ‘B’ in Campania and Basilicata), all of which differ in their sex chromosomes and autosomal polymorphisms, suggesting that P. hirtipes and P. italicum might each be a complex of cryptic species. 

FLUORESCENT PROBES OF CHROMOSOME STRUCTURE AND REPLICATION

1977 ◽  
Vol 19 (4) ◽  
pp. 603-623 ◽  
Author(s):  
Samuel A. Latt
Keyword(s):  
Chromosome Banding ◽  
Chromosome Structure ◽  
Fluorescent Dyes ◽  
Close Association ◽  
Replication Timing ◽  
Brdu Incorporation ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Chemical Studies ◽  
Chromosome Staining

Procedures employing fluorescent dyes or Giemsa stain have been utilized to differentiate metaphase chromosomes into longitudinal segments termed bands. In spite of the immense practical utility of chromosome banding, the chemical basis of banding patterns remains incompletely understood. Physical chemical studies have elucidated the modes and specificities of the interaction of fluorescent dyes such as quinacrine, 33258 Hoechst, daunomycin, chromomycin A3 and 7-aminoactinomycin D with DNA and chromatin. However, it is not clear that all aspects of chromosome staining are explainable in terms of the optical properties of soluble dye-DNA complexes. BrdU-dye techniques in which chromosome staining depends on the schedule of BrdU incorporation by cells, have been used for cytological studies of chromosome structure and replication. These procedures have revealed a close association between quinacrine or Giemsa bands and late replicating chromosomal regions. Biochemical studies on chromatin differentially labelled according to replication timing may thus prove useful for investigating the molecular basis of chromosome banding.

Differentiation of four taxa of the Anopheles balabacensis complex using H-banding patterns in the sex chromosomes

1984 ◽  
Vol 26 (4) ◽  
pp. 425-429 ◽  
Author(s):  
S. Wibowo ◽  
V. Baimai ◽  
R. G. Andre
Keyword(s):  
Sex Chromosomes ◽  
Species Differences ◽  
Constitutive Heterochromatin ◽  
Staining Technique ◽  
Hoechst 33258 ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Morphological Studies ◽  
Anopheles Balabacensis ◽  
Distinct Taxon

Analyses of metaphase chromosomes of four taxa of the Anopheles balabacensis complex (A. dirus A, B, and C, and A. takasagoensis) using the Hoechst 33258 staining technique have revealed remarkable differences in the fluorescence banding patterns of the sex chromosomes. These result from changes in the amount and distribution of constitutive heterochromatin. This evidence supports the results from cross-mating experiments and from morphological studies which indicate that three of these taxa, A. takasagoensis, dirus A, and dirus B, are sibling species. Differences in H-staining patterns of the sex chromosomes of a dirus colony from Kanchanaburi suggest that it too is a genetically distinct taxon, provisionally designated as dirus C, within the A. balabacensis complex.Key words: Anopheles, H-banding, heterochromatin, sex chromosomes.

Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

1995 ◽  
Vol 53 ◽  
pp. 932-933
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
R. Espinosa ◽  
M. M. Le Beau
Keyword(s):  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Analytical Sensitivity ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Sector Mass Spectrometer ◽  
Staining Methods ◽  
The University ◽  
Secondary Ion ◽  
Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

Neurulation in the posterior region of zebrafish, Danio rerio embryos

2021 ◽  
Author(s):  
Julia Gladysheva ◽  
Evdokia Evnukova ◽  
Ekaterina Kondakova ◽  
Milana Kulakova ◽  
Vladimir Efremov
Keyword(s):  
Danio Rerio ◽  
Posterior Region ◽  
Danio Rerio Embryos

scholarly journals Gene co-expression network analysis reveals mechanisms underlying ozone-induced carbamazepine toxicity in zebrafish (Danio rerio) embryos

Chemosphere ◽  
2021 ◽  
Vol 276 ◽  
pp. 130282
Author(s):  
Johannes Pohl ◽  
Oksana Golovko ◽  
Gunnar Carlsson ◽  
Stefan Örn ◽  
Monika Schmitz ◽  
...  
Keyword(s):  
Network Analysis ◽  
Danio Rerio ◽  
Danio Rerio Embryos

91. Studies on impact of chilling on sox gene expression in zebrafish (Danio rerio) embryos

Cryobiology ◽  
2010 ◽  
Vol 61 (3) ◽  
pp. 389
Author(s):  
K.K. Desai ◽  
E. Spikings ◽  
D.M. Rawson ◽  
T. Zhang
Keyword(s):  
Gene Expression ◽  
Danio Rerio ◽  
Sox Gene ◽  
Danio Rerio Embryos

CHROMOSOME STRUCTURE IN CHILOCORUS (COLEOPTERA: COCCINELLIDAE). II. THE ASYNCHRONOUS REPLICATION OF CONSTITUTIVE HETEROCHROMATIN

1976 ◽  
Vol 18 (1) ◽  
pp. 85-91 ◽  
Author(s):  
T. J. Ennis
Keyword(s):  
Chromosome Banding ◽  
Chromosome Structure ◽  
Constitutive Heterochromatin ◽  
Chromosome Replication ◽  
Data Models ◽  
Late Replication ◽  
Banding Patterns ◽  
Asynchronous Replication ◽  
Chilocorus Stigma

Chromosome replication has been analysed in four species of Chilocorus. In C. orbus Csy., C. tricyclus Smith, and C. hexacyclus Smith, centric regions of all chromosomes are last to replicate, preceded in order by heterochromatic arms and euchromatic arms. In C. stigma Say, very late replication of centric regions can be detected only in otherwise wholly euchromatic chromosomes (= monophasics); in chromosomes with one arm heterochromatic (= diphasics), these arms are last to replicate. Based on pachytene bivalent morphology and chromosome banding patterns, and supported by autoradiographic data, models are presented for the general organisation of Chilocorus chromosomes. All chromosomes in the first three species are subdivided into euchromatic arm, centric heterochromatin, and either a second euchromatic arm (monophasics) or a heterochromatic arm (diphasics). Chilocorus stigma diphasics apparently lack distinct centric organisation, and are therefore divided into euchromatic and heterochromatic arms only.

Short exposure to glyphosate induces locomotor, craniofacial, and bone disorders in zebrafish (Danio rerio) embryos

2021 ◽  
pp. 103700
Author(s):  
Rubén D. Díaz-Martín ◽  
Ana Carvajal-Peraza ◽  
Beatriz Yáñez-Rivera ◽  
Miguel Betancourt-Lozano
Keyword(s):  
Danio Rerio ◽  
Short Exposure ◽  
Bone Disorders ◽  
Danio Rerio Embryos
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