Cloning of a DNA repeat element from horse: DNA sequence and chromosomal localization

A DNA repeat element, revealed initially by digestion of horse DNA with TaqI, was cloned and characterized by Southern and in situ hybridization studies and nucleotide sequencing. The clone, e4/1, consisted of 32 tandem reiterations of a unit repeat of 21–22 bp, and produced multilocus DNA fingerprinting profiles that were useful for parentage analysis in horses. The tandem repeat element was shown by in situ hybridization to be localized in the centromeres of the acrocentric but not metacentric classes of horse chromosomes.Key words: Equidae, Equus caballus, DNA repeat, DNA fingerprinting, centromere.

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In situ hybridization in Actinidia using repeat DNA and genomic probes

Theoretical and Applied Genetics ◽

10.1007/s001220050444 ◽

1997 ◽

Vol 94 (3-4) ◽

pp. 507-513 ◽

Cited By ~ 12

Author(s):

G. Yan ◽

R. G. Atkinson ◽

A. R. Ferguson ◽

M. A. McNeilage ◽

B. G. Murray

Keyword(s):

In Situ Hybridization ◽

Repeat Dna ◽

Genomic Probes

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A human chromosome 9-specific alphoid DNA repeat spatially resolvable from satellite 3 DNA by fluorescent in situ hybridization

Genomics ◽

10.1016/0888-7543(91)90419-f ◽

1991 ◽

Vol 9 (3) ◽

pp. 517-523 ◽

Cited By ~ 45

Author(s):

Mariano Rocchi ◽

Nicoletta Archidiacono ◽

David C. Ward ◽

Antonio Baldini

Keyword(s):

In Situ Hybridization ◽

Human Chromosome ◽

Fluorescent In Situ Hybridization ◽

Chromosome 9 ◽

Dna Repeat ◽

Alphoid Dna

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Schistosoma mansoni: Chromosomal localization of DNA repeat elements by in situ hybridization using biotinylated DNA probes

Experimental Parasitology ◽

10.1016/0014-4894(89)90186-0 ◽

1989 ◽

Vol 69 (1) ◽

pp. 175-188 ◽

Cited By ~ 30

Author(s):

Hirohisa Hirai ◽

Loretta D. Spotila ◽

Philip T. LoVerde

Keyword(s):

In Situ Hybridization ◽

Schistosoma Mansoni ◽

Chromosomal Localization ◽

Dna Probes ◽

Repeat Elements ◽

Dna Repeat

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DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100122885 ◽

1992 ◽

Vol 50 (1) ◽

pp. 496-497

Author(s):

Barbara Trask ◽

Susan Allen ◽

Anne Bergmann ◽

Mari Christensen ◽

Anne Fertitta ◽

...

Keyword(s):

In Situ Hybridization ◽

Dna Sequence ◽

Dna Sequences ◽

Dual Band ◽

Nick Translation ◽

Metaphase Chromosomes ◽

Band Pass ◽

Texas Red ◽

Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

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Ultrastructural analysis of the spatial distribution of MRNA

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123167 ◽

1992 ◽

Vol 50 (1) ◽

pp. 552-553

Author(s):

Gary Bassell ◽

Robert H. Singer

Keyword(s):

Electron Microscopy ◽

Spatial Distribution ◽

In Situ Hybridization ◽

High Resolution ◽

Nucleic Acids ◽

Colloidal Gold ◽

Dna Probe ◽

Nucleotide Analogs ◽

Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

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