QTL analysis of morphological traits in an intergeneric BC1 progeny of Citrus and Poncirus under saline and non-saline environments

The effects of salinization with 40 mM sodium chloride on Poncirus trifoliata (L.) Raf., Citrus grandis (L.) Osb., their F1, and a BC1 progeny population [C. grandis × (F1)] were investigated by measuring growth and dry mass production of different tissues and by QTL (quantitative trait locus) mapping. A total of 36 traits related to growth (six traits) and tissue or whole plant dry mass production (30 traits) in salinized and non-salinized BC1 progeny clones were evaluated. The comparison of the three parental types to the BC1 progeny under control and saline conditions showed that the BC1 progeny plants segregated transgressively for many of these traits. Mapping analyses of these quantitative traits resulted in a total of 70 potential quantitative trait loci (PQTL) with LOD scores greater than or equal to 3.0 located on a previously generated linkage map. Sixty-nine percent of the mapped PQTLs were for traits associated with salinity. The small progeny population size used made further analyses of these PQTLs necessary. By considering LOD scores, map locations, and correlation analyses of the traits, it was possible to identify 16 regions of the citrus genome of interest, six of which were involved in both growth and dry mass production. Correlation analyses and locations of PQTLs indicated that many of these regions may contain one or a few genuine QTLs of large effect. This is a first step in identifying QTLs that have a major impact on growth and dry mass production in Citrus under both optimum and stressful environments.Key words: Citrus grandis, Poncirus trifoliata, salinity stress, QTL mapping, morphological traits, transgressive segregation, NaCl, sodium, chloride, salt tolerance, citrus genetics, breeding.

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QTL analysis of Na+ and Cl- accumulation related traits in an intergeneric BC1 progeny of Citrus and Poncirus under saline and nonsaline environments

Genome ◽

10.1139/g99-003 ◽

1999 ◽

Vol 42 (4) ◽

pp. 692-705 ◽

Cited By ~ 16

Author(s):

Ilhami Tozlu ◽

Charles L Guy ◽

Gloria A Moore

Keyword(s):

Quantitative Trait Loci ◽

Salt Tolerance ◽

Quantitative Trait ◽

Transgressive Segregation ◽

Poncirus Trifoliata ◽

Chloride Salt ◽

Correlation Analyses ◽

Whole Plant ◽

Trait Loci ◽

Citrus Genome

The effects of salinization with 40 mM NaCl on Poncirus trifoliata (L.) Raf., Citrus grandis (L.) Osb., their F1, and a BC1 progeny population (C. grandis × (F1)) were investigated by means of Na+ and Cl- analyses and QTL (quantitative trait loci) mapping. A total of 38 traits related to different tissue or whole-plant Na+ and (or) Cl- accumulation was analyzed in salinized and nonsalinized BC1 progeny clones. The comparison of the three parental types with the BC1 progeny under control and saline conditions showed that the BC1 progeny plants segregated transgressively for many traits. First mapping analyses resulted in a total of 73 potential quantitative trait loci (PQTL) with LOD scores [Formula: see text]3.0 located on a previously generated linkage map. Fifty-three percent of the mapped PQTLs were for traits associated with salinity. The small progeny population size used made further analyses of these PQTLs necessary. By considering LOD scores, map locations, and correlation analyses of the traits, it was possible to identify 17 regions of the citrus genome of interest: 8 of them may contain genuine QTLs of large effect and 9 regions are worthy of further study. Correlation analyses and locations of PQTLs indicated that many traits were controlled by fewer genes than the actual number of QTLs mapped for them. For example, 21 PQTLs mapped for Na+ accumulation and Cl-/Na+ ratios were located in a cluster at the beginning of one linkage group (LG), while 10 PQTLs mapped for Cl- accumulation and Cl-/Na+ ratios were located in a cluster at the beginning of another LG. This is the first step in identifying QTLs that have a major impact on salt tolerance and (or) mineral accumulation in citrus.Key words: Citrus grandis, Poncirus trifoliata, salinity stress, QTL mapping, transgressive segregation, mineral analysis, sodium, chloride, salt tolerance, citrus genetics.

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Regulation of growth and differential tissue dry mass accumulation by Citrus grandis, Poncirus trifoliata and their F1 under salinized and non-salinized environments

Functional Plant Biology ◽

10.1071/pp99060 ◽

2000 ◽

Vol 27 (1) ◽

pp. 27 ◽

Cited By ~ 1

Author(s):

Ilhami Tozlu ◽

Gloria A. Moore ◽

Charles L. Guy

Keyword(s):

Salinity Tolerance ◽

Mass Production ◽

Fine Root ◽

Dry Weight ◽

Dry Mass ◽

Poncirus Trifoliata ◽

Initial Growth ◽

Ion Content ◽

Average Growth ◽

Leaf Tissues

Salt stress responses of C. grandis L. (Osb.), P. trifoliata (L.) Raf. and their F 1 were investi-gated. Growth, growth rates, as well as leaf, stem, structural root (> 2 mm diameter), fine root (≤ 2mm diameter) and whole plant dry masses were determined for the three genotypes tested in 0, 40 and 80 mМ NaCl environments for 20 weeks. P. trifoliata and C. grandis were phenotypically distinct and their F1 had features that were a combination of both parents. The different growth habits resulted in significant differences between the net growth and growth ratios ([net growth / initial growth] × 100) of the three genotypes and between control and salinized plants within each genotype. The average growth and dry weights of nearly all tissues were reduced in salinized plants compared to those of control plants. The exceptions were the fine roots of P. trifoliata at both salinities and of the F1 plants at 40 mМ NaCl. The 40 mМ NaCl treatment stimulated fine root production in P. trifoliata plants, significantly increasing dry weight by 30% compared to control plants. Average shoot dry weight reduction was greatest in C. grandis and least in P. trifoliata in the 40 mМ NaCl treatment. While leaf tissues of P. trifoliata were the most sensitive to salinity, root tissues were the most sensitive in C. grandis. To avoid salt accumulation, P trifoliata plants increased root dry mass production while C. grandis plants increased leaf mass production. These traits appeared to be heritable, since the F1 plants displayed responses intermediate to its parents leading to increased salinity tolerance. We suggest that not only ion content of leaf tissues, but ion content and mass production of all tissues should be considered when the salinity tolerance of Citrus and related genera is characterized.

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Genetic mapping of quantitative trait locus for the leaf morphological traits in a recombinant inbred line population by ultra‐high–density maps across multi‐environments of maize ( Zea mays )

Plant Breeding ◽

10.1111/pbr.12749 ◽

2019 ◽

Vol 139 (1) ◽

pp. 107-118

Author(s):

Kuangye Zhang ◽

Xiangling Lv ◽

Fenghai Li ◽

Jia Wang ◽

Hongwei Yu ◽

...

Keyword(s):

Quantitative Trait Locus ◽

Zea Mays ◽

Genetic Mapping ◽

Recombinant Inbred Line Population ◽

Quantitative Trait ◽

Recombinant Inbred ◽

Morphological Traits ◽

Density Maps ◽

Trait Locus ◽

Leaf Morphological Traits

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Morphological Trait QTL Mapping in Tomato Recombinant Inbred Line Population

HortScience ◽

10.21273/hortsci.30.4.788d ◽

1995 ◽

Vol 30 (4) ◽

pp. 788D-788

Author(s):

I. Paran ◽

I.L. Goldman ◽

D. Zamir

Keyword(s):

Plant Height ◽

Quantitative Trait ◽

Recombinant Inbred ◽

Morphological Traits ◽

Recombinant Inbred Lines ◽

Rflp Analysis ◽

Leaf Length ◽

Node Number ◽

Marker Loci ◽

Trait Locus

Quantitative trait loci influencing morphological traits were identified by restriction fragment length polymorphism (RFLP) analysis in a population of recombinant inbred lines (RIL) derived from a cross of the cultivated tomato (Lycopersicon esculentum) with a related wild species (L. cheesmanii). One-hundred-thirty-two polymorphic RFLP loci spaced throughout the tomato genome were scored for 97 RIL families. Morphological traits, including plant height, fresh weight, node number, first flower-bearing node, leaf length at nodes three and four, and number of branches, were measured in replicated trials during 1991, 1992, and 1993. Significant (P ≤ 0.01 level) quantitative trait locus (QTL) associations of marker loci were identified for each trait. Lower plant height, more branches, and shorter internode length were generally associated with RFLP alleles from the L. cheesmanii parent. QTL with large effects on a majority of the morphological traits measured were detected at chromosomes 2, 3, and 4. Large additive effects were measured at significant marker loci for many of the traits measured. Several marker loci exhibited significant associations with numerous morphological traits, suggesting their possible linkage to genes controlling growth and development processes in Lycopersicon.

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