Identification of PCR-based markers flanking the recessive LMV resistance gene mo1 in an intraspecific cross in lettuce

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 982-986 ◽  
Author(s):  
S V Irwin ◽  
R V Kesseli ◽  
W Waycott ◽  
E J Ryder ◽  
J J Cho ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Resistance Gene ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Disease Resistance Gene ◽  
Symptom Development ◽  
Recessive Resistance ◽  
Lettuce Mosaic Virus ◽  
Recessive Resistance Gene ◽  
The Common

Molecular markers flanking the recessive resistance gene mo1 were identified following analysis of two segregating populations. Generation of a population from a crisphead × crisphead cross of Lactuca sativa that segregated for resistance conferred by the mo12 allele, but not morphological traits, was required for accurate mapping of the gene. Resistance was best assessed by symptom development after inoculating F3 families with the common pathotype II isolate of lettuce mosaic virus (LMV). Bulked segregant analysis for RAPD (random amplified polymorphic DNA) markers using one population, followed by linkage analysis using another, identified markers on either side of the mo1 gene, approximately 8 cM apart. These markers will be useful for more efficient introgression of this resistance gene into additional lettuce cultivars.Key words: lettuce, lettuce mosaic virus, molecular marker, disease resistance gene, bulked segregant analysis.

scholarly journals Inheritance of Resistance to Bean Golden Mosaic Virus in Common Bean

1998 ◽  
Vol 123 (4) ◽  
pp. 628-631 ◽  
Author(s):  
Jose J. Velez ◽  
Mark J. Bassett ◽  
James S. Beaver ◽  
Albeiro Molina
Keyword(s):  
Common Bean ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Recessive Gene ◽  
Gene Symbol ◽  
Inheritance Of Resistance ◽  
Recessive Resistance ◽  
Disease Response ◽  
Bean Golden Mosaic Virus ◽  
Recessive Resistance Gene

The inheritance of resistance to bean golden mosaic virus (BGMV) in common bean (Phaseolus vulgaris L.) was studied in crosses between susceptible bean variety XAN176 and resistant breeding lines 9236-6 (T446/A429) and 9245-94 (DOR303/T968). Disease response data were taken on plants from four generations derived from each cross (parents, F1, F2, and backcrosses (BCs) of F1 to both parents) at 25 days after plants were inoculated with BGMV, using whiteflies (Bemisia argentifolii Bellows & Perring) as vectors. The segregation ratios obtained from F2 and BC generations were consistent with the hypothesis that resistance in 9236-6, which prevents a chlorotic response, is conferred by a single recessive gene. The disease response in 9245-94 was controlled by two genes—a dominant gene controlling a dwarfing reaction and a recessive resistance gene preventing a chlorotic response to BGMV infection. An allelism test demonstrated that the gene controlling resistance in 9236-6 is nonallelic with the recessive gene controlling resistance in 9245-94. The gene symbol bgm is proposed for the recessive resistance gene (originally from A429) in 9236-6. The gene symbol bgm-2 is proposed for the recessive resistance gene (originally from DOR303) in 9245-94.

Impact of tobacco recessive resistance gene va on biological properties of Brazilian Potato virus Y (PVY) isolates

2011 ◽  
Vol 60 (6) ◽  
pp. 1048-1054 ◽  
Author(s):  
C. Lacroix ◽  
L. Glais ◽  
J.-L. Verrier ◽  
C. Charlier ◽  
C. Lorencetti ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Biological Properties ◽  
Recessive Resistance ◽  
Recessive Resistance Gene

Identification of coupling and repulsion phase RAPD markers for powdery mildew resistance gene er-1 in pea

Genome ◽  
1998 ◽  
Vol 41 (3) ◽  
pp. 440-444 ◽  
Author(s):  
K R Tiwari ◽  
G A Penner ◽  
T D Warkentin
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Rapd Markers ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Powdery Mildew Resistance Gene ◽  
Erysiphe Pisi ◽  
Mildew Resistance ◽  
Repulsion Phase

Powdery mildew is a serious disease of pea caused by the obligate parasite Erysiphe pisi Syd. Random amplified polymorphic DNA (RAPD) analysis has emerged as a cost-effective and efficient marker system. The objective of this study was to identify RAPD markers for powdery mildew resistance gene er-1. The resistant cultivar Highlight (carrying er-1) and the susceptible cultivar Radley were crossed, and F3 plants were screened with Operon (OP) and University of British Columbia (UBC) primers, using bulked segregant analysis. A total of 416 primers were screened, of which amplicons of three Operon primers, OPO-18, OPE-16, and OPL-6, were found to be linked to er-1. OPO-181200 was linked in coupling (trans to er-1) and no recombinants were found. OPE-161600 (4 ± 2 cM) and OPL-61900 (2 ± 2 cM) were linked in repulsion (cis to er-1). The fragments OPO-181200 and OPE-161600 were sequenced and specific primers designed. The specific primer pair Sc-OPO-181200 will be useful in identifying homozygous resistant individuals in F2 and subsequent segregating generations. Sc-OPE-161600 will have greatest utility in selecting heterozygous BC\dn6 nF1 individuals in backcross breeding programs.Key words: bulked segregant analysis,Erysiphe pisi, pea, RAPD.

scholarly journals Multiple Resistance Phenotypes to Lettuce mosaic virus Among Arabidopsis thaliana Accessions

2003 ◽  
Vol 16 (7) ◽  
pp. 608-616 ◽  
Author(s):  
Frédéric Revers ◽  
Thomas Guiraud ◽  
Marie-Christine Houvenaghel ◽  
Thierry Mauduit ◽  
Olivier Le Gall ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Recombinant Inbred Lines ◽  
Cell Movement ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Lettuce Mosaic Virus ◽  
Genetic Analyses ◽  
Large Variability

With the aim to characterize plant and viral factors involved in the molecular interactions between plants and potyviruses, a Lettuce mosaic virus (LMV)-Arabidopsis thaliana pathosystem was developed. Screening of Arabi-dopsis accessions with LMV isolates indicated the existence of a large variability in the outcome of the interaction, allowing the classification of Arabidopsis accessions into seven susceptibility groups. Using a reverse genetic approach, the genome-linked protein of LMV, a multifunc-tional protein shown to be involved in viral genome amplification and movement of potyviruses, was established as the viral determinant responsible for the ability to overcome the resistance of the Niederzenz accession to LMV-0. Preliminary genetic analyses from F2 and recombinant inbred lines available between susceptible and resistant Arabidopsis accessions revealed the existence of at least three resistance phenotypes to LMV with different genetic bases. One dominant resistance gene, designated LLM1, involved in blocking the replication or cell-to-cell movement of the LMV-0 isolate in the Columbia accession, was mapped to chromosome I and shown to be linked to the marker nga280. At the same time, genetic analyses of segregating F2 populations were consistent with the restriction of the systemic movement of the LMV-AF199 isolate in Columbia being controlled by two dominant genes and with the complete resistance to all tested LMV isolates of the Cape Verde islands (Cvi) accession being conferred by a single recessive resistance gene. Sequencing of the eu-karyotic translation initiation factor 4E genes from the different LMV-resistant Arabidopsis accessions showed that these factors are not directly involved in the characterized resistance phenotypes.

scholarly journals Identification of a Recessive Resistance Gene to Rice Bacterial Blight of Mutant Line XM6, Oryza sativa L.

1992 ◽  
Vol 42 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Satoru TAURA ◽  
Tsugufumi OGAWA ◽  
Atsushi YOSHIMURA ◽  
Ryoichi IKEDA ◽  
Nobuo lWATA
Keyword(s):  
Oryza Sativa ◽  
Resistance Gene ◽  
Bacterial Blight ◽  
Oryza Sativa L ◽  
Mutant Line ◽  
Recessive Resistance ◽  
Rice Bacterial Blight ◽  
Recessive Resistance Gene
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