THE USE OF DUBOS OLEIC ACID ALBUMIN MEDIUM ENRICHED WITH BLOOD FOR THE VIABLE COUNTING OF BCG

1956 ◽  
Vol 2 (3) ◽  
pp. 393-401 ◽  
Author(s):  
Doraine C. W. Thow
Keyword(s):  
Oleic Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Whole Blood ◽  
Bovine Serum ◽  
Freeze Drying ◽  
Maximum Size ◽  
Colony Count

Several different media were compared by means of surface viable counts of suspensions, in 0.1% solution of bovine serum albumin, of freshly grown BCG, and of BCG resuspended after freeze-drying. The growth of BCG on oleic acid albumin medium enriched with 5% whole blood was best in three respects: from a given inoculum, the colony count was highest; the colonies became visible soonest; and reached the greatest maximum size. The superiority of this medium was particularly evident with inocula of BCG reconstituted after freeze-drying.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

scholarly journals Interactions of the carboxyl group of oleic acid with bovine serum albumin: a 13C NMR study.

1983 ◽  
Vol 258 (15) ◽  
pp. 9262-9269 ◽  
Author(s):  
J S Parks ◽  
D P Cistola ◽  
D M Small ◽  
J A Hamilton
Keyword(s):  
Oleic Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
13C Nmr ◽  
Bovine Serum ◽  
Carboxyl Group ◽  
Nmr Study

Selection of a surrogate matrix for the quantification of an endogenous analyte in dried whole blood

Bioanalysis ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 409-418 ◽  
Author(s):  
Amy E Leaney ◽  
Carl Horner ◽  
Philip B Grace ◽  
Deborah H Mawson
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Whole Blood ◽  
Bovine Serum ◽  
Hydroxyvitamin D ◽  
Assay Performance ◽  
25 Hydroxyvitamin D ◽  
Surrogate Matrix ◽  
Phosphate Buffered Saline ◽  
Selection Of

Aim: A surrogate matrix is needed to quantify 25-hydroxyvitamin D3 in dried whole blood (DWB). To date, there has been limited guidance on approaches for quantification of endogenous analytes in atypical matrices, such as DWB. Methods: Different surrogate matrices were investigated in a systematic process using an LC–MS/MS assay. Performance assessment was made using quality controls of DWB with different hematocrits. Results & conclusion: Suitability of both phosphate-buffered saline containing bovine serum albumin and washed red blood cells recombined with phosphate-buffered saline containing bovine serum albumin as a surrogate matrix was demonstrated across a range of concentrations and hematocrits representative of expected endogenous analyte samples.

scholarly journals Bovine Serum Albumin as a Lyoprotectant for Preparation of DNA Dry Powder Formulations Using the Spray-Freeze Drying Method

2012 ◽  
Vol 35 (7) ◽  
pp. 1178-1181 ◽  
Author(s):  
Masashi Tsukamoto ◽  
Tomoyuki Okuda ◽  
Hirokazu Okamoto ◽  
Yuriko Higuchi ◽  
Shigeru Kawakami ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Freeze Drying ◽  
Drying Method ◽  
Dry Powder ◽  
Spray Freeze Drying ◽  
Dry Powder Formulations

Addition of oleic acid to delipidated bovine serum albumin aggravates renal damage in experimental protein-overload nephrosis

2005 ◽  
Vol 20 (11) ◽  
pp. 2349-2357 ◽  
Author(s):  
Mirjan M. van Timmeren ◽  
Stephan J. L. Bakker ◽  
Coen A. Stegeman ◽  
Rijk O. B. Gans ◽  
Harry van Goor
Keyword(s):  
Oleic Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Renal Damage
Sign in / Sign up

Export Citation Format

Share Document