Electron-microscopic study of Herpesvirus macaca in human fibroblasts

1976 ◽  
Vol 22 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Peter R. Graze ◽  
Phillip McGrath ◽  
Glenelle C. Washington ◽  
Ivor Royston
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Human Fibroblasts ◽  
Infectious Agent ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Enveloped Virus ◽  
Negative Stain ◽  
Infected Cells ◽  
Myelin Figures

An electron microscopic study of the morphology of Herpesvirus macaca, a serologically distinct infectious agent isolated from the leukocytes of rhesus monkeys, was performed. WI-38 fibroblast monolayers were infected with the virus and examined 18 days later. The morphology of Herpesvirus macaca was, in general, typical of the herpesvirus group. Enveloped virus particles observed via negative-stain technique had a diameter of 145–155 nm. An inner capsid composed of hexagonal capsomeres had a diameter of 100–110 nm and surrounded a central core. While enveloped forms appeared to be present within the nuclei of infected cells, they were not found in the cytoplasm except within vacuolar structures. Associated changes were found in the morphology of infected cells, including intracytoplasmic myelin figures.

The Growth of Herpesvirus mulatto in Human Fibroblast

1974 ◽  
Vol 32 ◽  
pp. 244-245
Author(s):  
Glennelle Washington ◽  
Philip P. McGrath ◽  
Peter R. Graze ◽  
Ivor Royston
Keyword(s):  
Rhesus Monkey ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Peripheral Blood ◽  
Human Fibroblast ◽  
Rhesus Monkeys ◽  
Human Fibroblasts ◽  
Electron Microscopic ◽  
Specific Antisera ◽  
Peripheral Blood Leucocytes

Herpes-like viruses were isolated from rhesus monkey peripheral blood leucocytes when co-cultivated with WI-38 cells. The virus was originally designated rhesus leucocyte-associated herpesvirus (LAHV) and subsequently called Herpesvirus mulatta (HVM). The original isolations were from juvenile rhesus monkeys shown to be free of antibody to rhesus cytomegalic virus. The virus could only be propagated in human or simian fibroblasts. Use of specific antisera developed from HVM showed no relationship between this virus and other herpesviruses. An electron microscopic study was undertaken to determine the morphology of Herpesvirus mulatta (HVM) in infected human fibroblasts.

Further Observations on the Virus of Epizootic Diarrhea of Infant Mice. An Electron Microscopic Study

1967 ◽  
Vol 25 ◽  
pp. 110-111
Author(s):  
W. G. Banfield ◽  
G. Kasnic ◽  
J. H. Blackwell
Keyword(s):  
Electron Microscope ◽  
Infected Cell ◽  
Microscopic Study ◽  
Intestinal Epithelium ◽  
Ultrastructural Study ◽  
Osmium Tetroxide ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Infected Cells

An ultrastructural study of the intestinal epithelium of mice infected with the agent of epizootic diarrhea of infant mice (EDIM virus) was first performed by Adams and Kraft. We have extended their observations and have found developmental forms of the virus and associated structures not reported by them.Three-day-old NLM strain mice were infected with EDIM virus and killed 48 to 168 hours later. Specimens of bowel were fixed in glutaraldehyde, post fixed in osmium tetroxide and embedded in epon. Sections were stained with uranyl magnesium acetate followed by lead citrate and examined in an updated RCA EMU-3F electron microscope.The cells containing virus particles (infected) are at the tips of the villi and occur throughout the intestine from duodenum through colon. All developmental forms of the virus are present from 48 to 168 hours after infection. Figure 1 is of cells without virus particles and figure 2 is of an infected cell. The nucleus and cytoplasm of the infected cells appear clearer than the cells without virus particles.

Laryngeal Papilloma: Etiologic and Therapeutic Considerations

1973 ◽  
Vol 82 (5) ◽  
pp. 649-655 ◽  
Author(s):  
Ted A. Cook ◽  
Arnold M. Cohn ◽  
J. Pierre Brunschwig ◽  
Helmuth Goepfert ◽  
Janet S. Butel ◽  
...  
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Surgical Excision ◽  
Therapeutic Trial ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Epidemiologic Factors ◽  
Therapeutic Program ◽  
Complete Surgical Excision ◽  
Tissue Specimens

A study of 13 patients with laryngeal papillomas is presented, with an analysis of epidemiologic factors, electron microscopic study of the lesion, and therapeutic trial of frequent microlaryngoscopic surgery and application of idoxuridine. Five of the nine children in this group were delivered by mothers who had condyloma accuminatum at time of delivery. These five children developed symptoms and required surgery much earlier than the other four. This correlation is shown to be statistically significant. The electron microscopic study of tissue specimens from all our patients failed to find any actual virus particles. The comparison study with skin and genital warts, however, showed ultrastructural details linking the three lesions. Results of the therapeutic program showed an overall improvement in frequency of recurrence, but no complete cures. This improvement is felt to be related to frequent complete surgical excision, and not to application of idoxuridine.

Electron Microscopic Study of the Morphogenesis of Echovirus 23

1973 ◽  
Vol 31 ◽  
pp. 590-591
Author(s):  
C. M. Trant ◽  
R. M. Jamison
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Post Infection ◽  
Glass Surface ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Monolayer Cultures ◽  
Infected Cells ◽  
Infectious Cycle

The cytopathology which accompanies the replication of Echovirus 23 in monkey kidney cells has been studied. Cells grown in monolayer cultures in glass bottles were infected with Echovirus 23 and incubated at 37°C. At varying intervals, cells were harvested for electron microscopy. Multiple embeddings of specimens at each interval post-infection were performed to confirm and extend the observations. It was noted that as the interval postinfection lengthened, individual infected cells rounded and detached from the surface of the monolayer. As the infectious cycle continued, virtually all cells in the bottle became detached. Accordingly, each infected bottle provided two specimens for electron microscopy; a) those free in the supernatent. fluid and b) those remaining on.the glass surface.

ELECTRON MICROSCOPIC STUDY ON VIRUS PARTICLES IN MAMMARY CARCINOMAS AND IN THEIR TRANSPLANTS AND CULTURED CELLS IN LOW-CANCER STRAIN MICE

Uirusu ◽  
1966 ◽  
Vol 16 (1-2) ◽  
pp. 6-12
Author(s):  
Kiyoshi HIRAKI ◽  
Zensuke OTA ◽  
Isao MIYOSHI ◽  
Hideo HARADA ◽  
Shinya SUZUKI ◽  
...  
Keyword(s):  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Cultured Cells ◽  
Electron Microscopic ◽  
Mammary Carcinomas ◽  
Virus Particles
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