The effect of inorganic phosphate on cyclic adenosine 3′, 5′-monophosphate in streptomycin-producing cultures of Streptomyces griseus

1981 ◽  
Vol 27 (10) ◽  
pp. 1044-1047 ◽  
Author(s):  
J. Terry ◽  
D. G. Springham
Keyword(s):  
Cyclic Amp ◽  
Inorganic Phosphate ◽  
Streptomyces Griseus ◽  
Phosphate Concentration ◽  
Concentration Addition ◽  
Defined Media ◽  
Streptomycin Biosynthesis

Intracellular and extracellular cyclic adenosine 3′, 5′-monophosphate (cyclic AMP) levels were measured during the growth of a streptomycin-producing strain of Streptomyces griseus. Increasing the inorganic phosphate concentration of the defined media resulted in a decreased streptomycin titre and alterations in the magnitude and timing of changes in cyclic AMP concentration. Addition of excess inorganic phosphate after 24 h of growth reduced steptomycin production but had no effect on the intracellular cyclic AMP concentration. The results do not suggest that the effect of inorganic phosphate on streptomycin biosynthesis is mediated by cyclic AMP.

Effect of exogenous nucleotides on the candicidin fermentation

1977 ◽  
Vol 23 (10) ◽  
pp. 1334-1339 ◽  
Author(s):  
Juan F. Martin ◽  
Arnold L. Demain
Keyword(s):  
Protein Synthesis ◽  
Cyclic Amp ◽  
Cyclic Nucleotides ◽  
Inorganic Phosphate ◽  
Streptomyces Griseus ◽  
Cell System ◽  
Phosphate Ester ◽  
Aminobenzoic Acid ◽  
Inhibit Protein Synthesis ◽  
Resting Cell

Addition of cyclic-AMP (c-AMP) to Streptomyces griseus fermentations inhibited candicidin formation. In a phosphate-free resting cell system, c-AMP inhibited net candicidin formation and incorporation of labeled propionate and p-aminobenzoic acid into the antibiotic but did not inhibit protein synthesis. All nucleotides tested, regardless of the position of the phosphate ester, were effective inhibitors; nucleosides and free bases were not. Inhibition occurred whether the nucleotide was added early or late. The results indicate that inhibition of antibiotic formation by exogenous nucleotides, including cyclic nucleotides, is similar to the effect produced by inorganic phosphate.

PLASMA INORGANIC PHOSPHATE CONCENTRATION AND HYPERVENTILATION IN THE DOG

1948 ◽  
Vol 154 (2) ◽  
pp. 185-187 ◽  
Author(s):  
Gilbert S. Campbell ◽  
E. B. Brown ◽  
Frank Gollan
Keyword(s):  
Inorganic Phosphate ◽  
Phosphate Concentration

The Effect of Calcium and Phosphate on the Biphasic Calcium Uptake by the Sarcoplasmic Reticulum

1975 ◽  
Vol 30 (11-12) ◽  
pp. 777-780 ◽  
Author(s):  
Pierre Ermier ◽  
Wilhelm Hasselbach
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Inorganic Phosphate ◽  
Calcium Uptake ◽  
Initial Rate ◽  
Maximum Amplitude ◽  
Phosphate Concentration ◽  
Free Calcium ◽  
Physiological Range ◽  
External Calcium

Abstract The amplitude of the fast uptake and the initial rate of the slow uptake increase with in­ creasing free calcium concentrations, up to 30 μᴍ. In that range, both processes are correlated to each other. At higher concentrations, the slow uptake is more inhibited than the fast uptake. The fast uptake shows a maximum amplitude which remains unchanged in the presence of phosphate. The slow uptake leads to a nearly complete depletion of the external calcium, and its rate is proportional to the phosphate concentration, even at physiological range. The sarcoplasmic ATPase liberates inorganic phosphate and the slow uptake

POLYHYDRIC ALCOHOL PRODUCTION BY OSMOPHILIC YEASTS: EFFECT OF OXYGEN TENSION AND INORGANIC PHOSPHATE CONCENTRATION

1957 ◽  
Vol 3 (4) ◽  
pp. 559-567 ◽  
Author(s):  
J. F. T. Spencer ◽  
Ping Shu
Keyword(s):  
Oxygen Tension ◽  
Inorganic Phosphate ◽  
Opposite Effect ◽  
Ethanol Yield ◽  
Phosphate Concentration ◽  
Alcohol Production ◽  
Lower Oxygen ◽  
Oxygen Tensions ◽  
Effect Of Oxygen ◽  
Very High

A study was made of the effects of oxygen tension and inorganic phosphate concentration on the production of glycerol, arabitol, and ethanol by a strain of osmophilic yeast. Increasing the oxygen tension increased the yields of glycerol and arabitol and decreased the yield of ethanol, while increasing the concentration of inorganic phosphate had the opposite effect.The changes in yields of products with increasing phosphate concentration were most pronounced at a partial pressure of oxygen of 280 mm. of mercury. At lower oxygen tensions the yields of polyols were low and the yield of ethanol was high at all concentrations of phosphate, while at very high oxygen tensions the opposite effect was observed. When the ethanol yield was high, the respiratory quotient was also high. These results are discussed in relation to the oxidative phosphate-lack theory of the Pasteur effect.

Arbuscular mycorrhizal responsiveness of in vitro tomato root lines is not related to growth and nutrient uptake rates

2003 ◽  
Vol 81 (7) ◽  
pp. 645-656 ◽  
Author(s):  
Karine Labour ◽  
Mario Jolicoeur ◽  
Marc St-Arnaud
Keyword(s):  
Nutrient Uptake ◽  
Hairy Root ◽  
Hairy Roots ◽  
Inorganic Phosphate ◽  
Minimal Medium ◽  
Glomus Intraradices ◽  
Phosphate Concentration ◽  
Tomato Cultivar ◽  
Uptake Rates

Variability in growth and nutritional dynamics of in vitro tomato hairy root lines and their relationship with responsiveness to mycorrhizal colonization were studied. Four tomato cultivars were transformed with three Agrobacterium rhizogenes strains to obtain several hairy root lines, which were compared for growth and receptivity to Glomus intraradices. Four transformed hairy root lines were further characterized and compared with excised roots of the nontransformed tomato cultivar 'Cobra' and with Ri-T-DNA carrot hairy roots. Lines were compared during 4 months on minimal medium in terms of growth, nutrient uptake, and mycorrhizal colonization. In a subexperiment, the cultures were grown on a modified minimal medium to assess the contribution of initial inorganic phosphate concentration in mycorrhizal susceptibility of the three initially nonreceptive lines. On minimal medium, growth and nutrient uptake rates were highly correlated, but both were unrelated to mycorrhizal receptiveness. All the lines successfully established the symbiosis when the initial phosphate concentration was significantly reduced. No association was found between the origin of lines from the different tomato cultivar – bacterial strain combinations and the absence of symbiosis establishment on minimal medium. Decrease of inorganic phosphate concentration at the beginning of the culture was a key factor involved in precolonization steps of mycorrhizal symbiosis.Key words: Glomus intraradices, hairy roots, Lycopersicon esculentum, mycorrhizal responsiveness, root nutrition, inorganic phosphate.

Absorption of Inorganic Phosphate in the Human Small Intestine

1979 ◽  
Vol 56 (5) ◽  
pp. 407-412 ◽  
Author(s):  
J. Walton ◽  
T. K. Gray
Keyword(s):  
Small Intestine ◽  
Inorganic Phosphate ◽  
Water Movement ◽  
Human Subjects ◽  
Sodium Concentration ◽  
Phosphate Concentration ◽  
Phosphate Absorption ◽  
Human Small Intestine ◽  
Intestinal Phosphate Absorption

1. Intestinal phosphate absorption in human subjects was studied by the technique of triple lumen intestinal perfusion in vivo. 2. Ileal phosphate absorption increased as the intraluminal phosphate concentration was increased. 3. Ileal rates of phosphate absorption were lower at any given intraluminal phosphate concentration than previously described jejunal rates. Acidification of the ileal lumen did not increase phosphate absorption. 4. Phosphate absorption was shown in the jejunum to be dependent on the intraluminal sodium concentration. 5. Phosphate absorption in the human small intestine consists of at least two components, one directly proportional to water movement and the second apparently independent of water movement.

scholarly journals Dissolved Inorganic Phosphate Concentration in Stream Water in Japan and Factors Controlling the Concentration

2006 ◽  
Vol 29 (11) ◽  
pp. 679-686 ◽  
Author(s):  
Takashi WAKAMATSU ◽  
Eiichi KONOHIRA ◽  
Junko SHINDO ◽  
Takahito YOSHIOKA ◽  
Katsuo OKAMOTO ◽  
...  
Keyword(s):  
Inorganic Phosphate ◽  
Stream Water ◽  
Phosphate Concentration
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