Use of the enzyme-linked immunosorbent assay (ELISA) in the detection of Rhizobium both in culture and from root nodules of soybeans and cowpeas

1985 ◽  
Vol 31 (6) ◽  
pp. 524-528 ◽  
Author(s):  
S. Asanuma ◽  
G. Thottappilly ◽  
A. Ayanaba ◽  
V. Ranga Rao
Keyword(s):  
Silica Gel ◽  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Root Nodules ◽  
Enzyme Linked Immunosorbent Assay ◽  
Double Antibody ◽  
Laboratory Conditions ◽  
Direct Elisa ◽  
Rhizobium Sp

The enzyme-linked immunosorbent assay (ELISA) was used to detect Rhizobium sp. in the "cowpea" group and R. japonicum both in culture and from nodules of glasshouse and field-grown plants. Double antibody sandwich (direct ELISA) and indirect ELISA were found to be equally sensitive in detecting rhizobia under controlled laboratory conditions. It was found that nodules preserved by freezing or drying over silica gel were equally good. No loss in sensitivity was detected when nodules were crushed directly in the microplates.

scholarly journals PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER

2015 ◽  
Vol 9 (1) ◽  
Author(s):  
Indrawati Sendow ◽  
R.M. Abdul Adjid ◽  
Atik Ratnawati ◽  
Muharam Saepulloh
Keyword(s):  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blocking Elisa ◽  
Bovine Ephemeral Fever ◽  
Direct Elisa

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.

Enzyme-Linked Immunosorbent Assay for Deoxynivalenol in Corn and Wheat

1988 ◽  
Vol 71 (5) ◽  
pp. 945-949 ◽  
Author(s):  
Yi-Chun XU ◽  
Guang S Zhang ◽  
Fun S Chu
Keyword(s):  
Acetic Anhydride ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Immunosorbent Assay ◽  
Phosphate Buffer ◽  
Indirect Elisa ◽  
Enzyme Linked Immunosorbent Assay ◽  
Detection Level ◽  
Direct Elisa ◽  
Layer Chromatography

Abstract The availability of antibody against deoxynivalenol (DON) triacetate (Tri-Ac-DON) has enabled development of a direct enzyme-linked immunosorbent assay (ELISA) and an indirect ELISA for DON in corn and wheat. In both assays, DON is extracted from the sample with acetonitrile- water, reacted with acetic anhydride to form Tri- Ac-DON, and diluted in phosphate buffer for analysis. Direct ELISA was found to be the more sensitive procedure. Fewer interferences are evidenced, and the assay is less time consuming than is indirect ELISA. For direct ELISA, recovery of 10-1000 ppb DON added to corn and wheat was 100% (SD 15, CV 15%) and 102.1% (SD 12.2, CV 11.9%), respectively. For indirect ELISA, overall recovery of 10- 1000 ppb DON added to wheat was 121.5% (SD 39.5, CV 32.5%); in the higher concentration range (500-1000 ppb), recovery was 105% (SD 18, CV 17%). The minimal detection level for DON was around 10 ppb. Analysis of 7 naturally contaminated samples for DON showed that the ELISA results agreed well with those obtained by radioimmunoassay and thin-layer chromatography.

A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model

2012 ◽  
Vol 130 (2) ◽  
pp. 178-182 ◽  
Author(s):  
Yuzi Luo ◽  
Mohamad Alaa Terkawi ◽  
Honglin Jia ◽  
Gabriel Oluga Aboge ◽  
Youn-Kyoung Goo ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Babesia Microti ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hamster Model ◽  
Double Antibody

A modified double antibody sandwich enzyme-linked immunosorbent assay for measurement of alpha-1-antitrypsin in biologic fluids

1985 ◽  
Vol 83 (1) ◽  
pp. 101-112 ◽  
Author(s):  
Joseph P. Michalski ◽  
Candace C. McCombs ◽  
Sangita Sheth ◽  
Maria McCarthy ◽  
Richard deShazo
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Double Antibody ◽  
Alpha 1 Antitrypsin

Simultaneous Analysis of T-2 Toxin and HT-2 Toxin by an Indirect Enzyme-Linked Immunosorbent Assay

1987 ◽  
Vol 70 (4) ◽  
pp. 657-661
Author(s):  
Titan S L Fan ◽  
Yi-Chun Xu ◽  
Fun Sun Chu
Keyword(s):  
Immunosorbent Assay ◽  
Indirect Elisa ◽  
Simultaneous Analysis ◽  
Urine Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Toxin A ◽  
Toxin Concentration ◽  
Mixed Sample ◽  
Analytical Recovery ◽  
The Individual

Abstract An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of HT-2 toxin in the presence or absence of T-2 toxin is described. In the indirect ELISA, the relative cross-reactivities of antibodies against T-2 toxin (anti-T-2) with T-2 toxin and HT-2 toxin were 1 and 0.1, whereas anti-HT-2 cross-reactivities with T-2 toxin and HT-2 toxin were 0.33 and 1, respectively. Using such relationships, a formula was established that could be used to calculate the individual toxin concentration in a mixed sample after experimentally analyzing for T-2 and HT-2 toxins in the 2 indirect ELISAs. This method was tested by analyzing urine samples spiked with HT-2 toxin alone and samples spiked with both T-2 toxin and HT-2 toxin. A cleanup protocol for treatment of urine samples before ELISA was also established. The overall analytical recovery of HT-2 toxin when it was added at concentrations of 0.1-10 parts per billion (ppb) to the urine samples was ca 89%. When both T-2 and HT-2 toxins were added to the urine samples at equal concentrations of 0.5 to 5.0 ppb, their recoveries were 112 and 109%, respectively.

Immuno-enzymatic assay for the detection of Schistosoma mansoni antigens in serum of mice harbouring bisexual or unisexual light worm infections

1979 ◽  
Vol 53 (3) ◽  
pp. 189-194 ◽  
Author(s):  
A. W. Ferreira ◽  
A. L. M. Caldini ◽  
S. Hoshino-Shimizu ◽  
M. E. Camargo
Keyword(s):  
Schistosoma Mansoni ◽  
Immunosorbent Assay ◽  
Enzymatic Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Male Worm ◽  
Antibody Technique ◽  
Post Exposure ◽  
Double Antibody

ABSTRACTEnzyme-linked immunosorbent assay (ELISA) double antibody technique was standardized for detecting S. mansoni Polysaccharide and protein antigens in serum of infected mice. Anti-sera specific for either worm components were obtained in sheep and peroxidase conjugates prepared from each serum. The immunoenzimatic test for protein could detect as little as 6μg/ml antigens, and the test for polysaccharides 3 μg/ml.Both bisexual and unisexual male worm low infections were produced, and studied for as long as 27 weeks post-exposure. Worm components were found in serum from both types of infections and in progressively higher percentages of animals until the end of the 27 weeks observations period. For unisexual male infections this percentage reached from about 50% to 60% of mice, and 100% for bisexual infections. Significantly higher antigen concentrations in serum were found at 27 weeks for bisexual infections, no antigen increase being detected in relation to starting egg secretion, which occurred at 5 week infections.

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