Report on the 53rd Annual Meeting of the Canadian Society of Biochemistry, Molecular and Cellular Biology: “Membrane Proteins in Health and Disease”

The meeting “Membrane Proteins in Health and Disease” featured 6 sessions and 2 satellite meetings. At the opening session, Gunnar von Heijne delivered a plenary lecture entitled Insertion of Membrane Proteins into the Endoplasmic Reticulum. The following session topics were Membrane Protein Trafficking and Folding, Regulation of Membrane Proteins, Membrane Protein Structure, Membrane Proteins in Diverse Species, and Membrane Proteins and Diseases. The satellite meetings discussed bicarbonate transporters and Na+/H+ exchangers. Together the 21 lectures and 106 posters presented at the meeting spanned the full spectrum of current research into membrane protein structure and function.

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Order-disorder transitions of cytoplasmic N-termini in the mechanisms of P-type ATPases

Faraday Discussions ◽

10.1039/d0fd00040j ◽

2020 ◽

Author(s):

Khondker Rufaka Hossain ◽

Daniel Clayton ◽

Sophia C Goodchild ◽

Alison Rodger ◽

Richard James Payne ◽

...

Keyword(s):

Protein Structure ◽

Membrane Protein ◽

Structure And Function ◽

Membrane Protein Structure ◽

Protein Structure And Function ◽

Lipid Environment ◽

Membrane Pumps ◽

And Function ◽

P Type

Membrane protein structure and function are modulated via interactions with their lipid environment. This is particularly true for the integral membrane pumps, the P-type ATPases. These ATPases play vital roles...

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Nanoscale lipid membrane mimetics in spin-labeling and electron paramagnetic resonance spectroscopy studies of protein structure and function

Nanotechnology Reviews ◽

10.1515/ntrev-2016-0080 ◽

2017 ◽

Vol 6 (1) ◽

pp. 75-92 ◽

Cited By ~ 6

Author(s):

Elka R. Georgieva

Keyword(s):

Electron Paramagnetic Resonance ◽

Protein Structure ◽

Membrane Proteins ◽

Membrane Protein ◽

Spin Labeling ◽

Structure And Function ◽

Protein Structure And Function ◽

Paramagnetic Resonance ◽

And Function ◽

Electron Paramagnetic

AbstractCellular membranes and associated proteins play critical physiological roles in organisms from all life kingdoms. In many cases, malfunction of biological membranes triggered by changes in the lipid bilayer properties or membrane protein functional abnormalities lead to severe diseases. To understand in detail the processes that govern the life of cells and to control diseases, one of the major tasks in biological sciences is to learn how the membrane proteins function. To do so, a variety of biochemical and biophysical approaches have been used in molecular studies of membrane protein structure and function on the nanoscale. This review focuses on electron paramagnetic resonance with site-directed nitroxide spin-labeling (SDSL EPR), which is a rapidly expanding and powerful technique reporting on the local protein/spin-label dynamics and on large functionally important structural rearrangements. On the other hand, adequate to nanoscale study membrane mimetics have been developed and used in conjunction with SDSL EPR. Primarily, these mimetics include various liposomes, bicelles, and nanodiscs. This review provides a basic description of the EPR methods, continuous-wave and pulse, applied to spin-labeled proteins, and highlights several representative applications of EPR to liposome-, bicelle-, or nanodisc-reconstituted membrane proteins.

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Structures of membrane proteins

Quarterly Reviews of Biophysics ◽

10.1017/s0033583510000041 ◽

2010 ◽

Vol 43 (1) ◽

pp. 65-158 ◽

Cited By ~ 87

Author(s):

Kutti R. Vinothkumar ◽

Richard Henderson

Keyword(s):

Protein Structure ◽

Membrane Proteins ◽

Membrane Protein ◽

Protein Structures ◽

Protein Structure Determination ◽

Membrane Protein Structure ◽

Expression Systems ◽

Genome Sequences ◽

Conformational Variability ◽

Efficient Expression

AbstractIn reviewing the structures of membrane proteins determined up to the end of 2009, we present in words and pictures the most informative examples from each family. We group the structures together according to their function and architecture to provide an overview of the major principles and variations on the most common themes. The first structures, determined 20 years ago, were those of naturally abundant proteins with limited conformational variability, and each membrane protein structure determined was a major landmark. With the advent of complete genome sequences and efficient expression systems, there has been an explosion in the rate of membrane protein structure determination, with many classes represented. New structures are published every month and more than 150 unique membrane protein structures have been determined. This review analyses the reasons for this success, discusses the challenges that still lie ahead, and presents a concise summary of the key achievements with illustrated examples selected from each class.

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