Some features of the optical rotatory dispersion spectrum of the α-lytic protease of Sorangium sp.
Keyword(s):
A study of the kinetic properties of the α-lytic protease of Sorangium sp. indicated that substrate-binding by the enzyme was not pH dependent. In agreement with this indication of a pH-insensitive conformation, the optical rotation of the enzyme between pH 5 and 10.5 is not pH dependent. The optical rotatory dispersion spectrum above 220 mμ shows a main Cotton effect with a trough at 230 mμ and small but well-marked Cotton effects between 260 and 300 mμ. The reduced, mean residue rotation at the trough of the main Cotton effect was estimated to be −1650 ± 80° cm2/dmole; the Moffitt–Yang parameter b0 for rotations above 325 mμ is approximately zero. These values suggest that the enzyme has virtually no α-helices.
1929 ◽
Vol 122
(789)
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pp. 245-250
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1966 ◽
Vol 0
(0)
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pp. 1177-1183
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1965 ◽
Vol 87
(1)
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pp. 66-72
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1970 ◽
Vol 7
(1)
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pp. 8-16
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