COUPLED GAS LIQUID – THIN LAYER CHROMATOGRAPHY: SIMULTANEOUS DETERMINATION OF PIPERITONE AND PIPERITONE OXIDE IN ESSENTIAL OILS

Gas liquid and thin layer chromatography have been combined into a single microphysicochemical technique. The resolution of two terpenoids found in nature—piperitone and piperitone oxide—is described to illustrate the scope of the novel method of analysis. Occurrence of the epoxide in Mentha arvensis (Japanese mint), Mentha piperita (Mitcham peppermint), Eucalyptus dives Schauer, "Type" ("broad-leaved peppermint"), and Eucalyptus numerosa Maiden, var. "A" is reported for the first time. Its presence in these species should prove of biochemical as well as taxonomic interest.

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Critical thin-layer chromatography as a novel method for the determination of the molecular weight and compositional inhomogeneity of block copolymers

Journal of Chromatography A ◽

10.1016/0021-9673(92)80180-3 ◽

1992 ◽

Vol 609 (1-2) ◽

pp. 355-362 ◽

Cited By ~ 23

Author(s):

B.G. Belenkii ◽

E.S. Gankina ◽

V.N. Zgonnik ◽

I.I. Malchova ◽

E.U. Melenevskaya

Keyword(s):

Molecular Weight ◽

Block Copolymers ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Novel Method ◽

Layer Chromatography

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Determination of curcumin in bouillon cubes by high-performance thin-layer chromatography

Zurnal Hromatograficnogo tovaristva ◽

10.15407/zht2020.66.004 ◽

2021 ◽

Vol 20 (66) ◽

pp. 4-14

Author(s):

A Yegorova ◽

◽

Yu Loskutova ◽

G Voitiuk ◽

A Maltsev ◽

...

Keyword(s):

Detection Limit ◽

Thin Layer ◽

Thin Layer Chromatography ◽

Calibration Curve ◽

High Performance ◽

Food Industries ◽

Method Accuracy ◽

First Time ◽

Layer Chromatography

Currently, high-performance thin-layer chromatography (HPTLC) is widely used to control product quality in the pharmaceutical and food industries. In the manufacture of food products, the most important requirement is the control of the content of various additives (preservatives, dyes, antioxidants). For the first time, a method for determining curcumin by high-performance thin-layer chromatography in bouillon cubes "Gallina Blanca" was proposed. Detection was carried out by densitometric scanning using CAMAG equipment when measuring absorbance at a wavelength 265 nm. The method is based on determining the peak area of curcumin in the chromatogram depending on its concentration. Curcumin content is determined by the calibration curve. The developed method was validated by the following tests: specificity, linearity, accuracy and detection limit. The calibration curve is linear in the range of curcumin concentrations from 120 to 520 ng/spot, the detection limit is 65 ng/spot. The specificity of the method is based on the ability to unambiguously evaluate the analyte in the presence of other components and is confirmed by using an external standard. The spots on the chromatograms of the test solution and the reference solution coincide in the value of Rf, which confirms the specificity of the method. Accuracy was evaluated according to the results of the analysis of various samples. The requirement for statistical insignificance of systematic error is fulfilled. The proposed method is express, characterized by satisfactory metrological characteristics, ease of implementation. Key words: high-performance thin-layer chromatography, curcumin, validation.

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