Structure of the Capsular Polysaccharide of Klebsiella K-type 20

1973 ◽  
Vol 51 (18) ◽  
pp. 3015-3020 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton
Keyword(s):  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Acetyl Group ◽  
Unit Formula

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer, of Klebsiella K20 show the structure to consist of a repeating unit[Formula: see text]The anomeric linkages were determined by p.m.r. spectroscopy of the carboxyl reduced polysaccharide, and periodate degraded polysaccharides. The p.m.r. spectroscopy of the original polysaccharide also showed the presence in the polysaccharide of one O-acetyl group per eight sugar residues.

The Structure of the Capsular Polysaccharide of Klebsiella K-type 24

1973 ◽  
Vol 51 (11) ◽  
pp. 1819-1825 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton ◽  
Alberto M. Zanlungo
Keyword(s):  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Acetyl Group ◽  
Unit Formula

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer, of Klebsiella K24 show the structure to consist of a repeating unit[Formula: see text]The anomeric linkages were determined in isolated oligosaccharides by p.m.r. spectroscopy which also showed the presence in the polysaccharide of one O-acetyl group per seven or eight sugar residues. The O-acetyl is tentatively assigned to one of the D-mannose units.

4,6-O-(1-Carboxyethylidene)-D-mannose as a Structural Unit in Capsular Polysaccharide of Klebsiella K-type 5

1972 ◽  
Vol 50 (14) ◽  
pp. 2382-2384 ◽  
Author(s):  
G. G. S. Dutton ◽  
M. T. Yang
Keyword(s):  
Experimental Data ◽  
Structural Unit ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Acetyl Group

Methylation, periodate oxidation, and partial hydrolysis techniques have each been used to demonstrate the presence of 4,6-O-(1-carboxyethylidene)-D-mannopyranosyl units in the capsular polysaccharide of Klebsiella K-type 5. The structure of this polysaccharide differs from those known for other Klebsiella capsules by the lack of any carbohydrate side chain. A repeating unit of[Formula: see text](plus one unassigned O-acetyl group) is in accord with the experimental data.

The Structure of the Capsular Polysaccharide of Klebsiella K-type 5

1973 ◽  
Vol 51 (11) ◽  
pp. 1826-1832 ◽  
Author(s):  
Guy G. S. Dutton ◽  
Mo-Tai Yang
Keyword(s):  
Molecular Weight ◽  
Capsular Polysaccharide ◽  
Gel Filtration ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Unit Formula

Methylation, periodate oxidation and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer of Klebsiella K5 show the structure to consist of a repeating unit:[Formula: see text]The polysaccharide has a molecular weight (by gel filtration) of 9 × 105 and is the first Klebsiella capsule to be found lacking a carbohydrate side chain. The proportion of O-acetyl and pyruvate groups was determined by p.m.r. spectroscopy.

The Structure of the Capsular Polysaccharide from Klebsiella K-Type 21

1973 ◽  
Vol 51 (2) ◽  
pp. 198-207 ◽  
Author(s):  
Y. M. Choy ◽  
G. G. A. Dutton
Keyword(s):  
Pyruvic Acid ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Methyl Sulfoxide ◽  
Unit Formula

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer, of Klebsiella K21 show the structure to consist of a repeating unit.[Formula: see text]The anomeric linkages were determined by p.m.r. spectroscopy of isolated oligosaccharides and, in part, by specific enzymes. P.m.r. spectroscopy of the original polysaccharide in methyl sulfoxide-d6 showed clearly a ratio of one pyruvic acid ketal (CH3, τ 8.5) to five anomeric protons (τ 4.65–5.40).

Structure of the Capsular Polysaccharide of Klebsiella K-type 56

1973 ◽  
Vol 51 (18) ◽  
pp. 3021-3026 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton
Keyword(s):  
Uronic Acid ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Hydrolysis Of

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide of Klebsiella K-type 56 show the structure to be a repeating unit consisting of[Formula: see text]The nature of the anomeric linkages was determined by p.m.r. spectroscopy of isolated oligosaccharides. The position of the L-rhamnose side chain was defined by characterization of the di- and tetrasaccharides obtained by partial hydrolysis of the fully methylated polysaccharide.This structure represents the first capsular polysaccharide lacking uronic acid to be studied in the genus Klebsiella.

Structure of the capsular polysaccharide antigen of type IV group B Streptococcus

1989 ◽  
Vol 67 (5) ◽  
pp. 877-882 ◽  
Author(s):  
Jose L. Di Fabio ◽  
Francis Michon ◽  
Jean-Robert Brisson ◽  
Harold J. Jennings ◽  
Michael R. Wessels ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Molar Ratio ◽  
Partial Hydrolysis ◽  
Spectroscopic Techniques ◽  
Polysaccharide Antigen ◽  
Type Iv ◽  
High Resolution Nmr ◽  
Group B ◽  
Unit Formula

The native polysaccharide antigen isolated from type IV group B Streptococcus contains D-galactose, D-glucose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in the molar ratio 2:2:1:1 and is composed of the following repeating unit:[Formula: see text]The structural analysis of this antigen was based on results obtained from methylation analysis, partial hydrolysis, nitrous acid deamination, and nuclear magnetic resonance spectroscopic techniques. Keywords: capsular polysaccharide, high resolution NMR spectroscopy, group B Streptococcus, polysaccharide structure, polysaccharide degradation.

The Structure of the Capsular Polysaccharide of Klebsiella K-type 72; Occurrence of 3,4-O-(1-Carboxyethylidene)-L-rhamnose

1974 ◽  
Vol 52 (4) ◽  
pp. 684-687 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton
Keyword(s):  
Capsular Polysaccharide ◽  
Partial Hydrolysis ◽  
Unit Formula

Methylation and partial hydrolysis studies on the capsular polysaccharide of Klebsiella K72 show the structure to consist of a repeating unit[Formula: see text]The anomeric linkages were determined in isolated oligosaccharides by p.m.r. spectroscopy which also showed the presence in the polysaccharide of one pyruvate ketal per four sugar residues.

Structure of the specific capsular polysaccharide of Streptococcus pneumoniae type 23F (American type 23)

1988 ◽  
Vol 66 (7) ◽  
pp. 758-771 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
High Resolution ◽  
Capsular Polysaccharide ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Composition Analysis ◽  
Part D ◽  
Magnetic Resonance Studies

The specific capsular polysaccharide of Streptococcus pneumoniae serotype 23F (American type 23) is composed of a repeating tetrasaccharide unit containing D-glucose (one part), D-galactose (one part), L-rhamnose (two parts), glycerol (one part), and phosphate (one part). By composition analysis, optical rotation, partial hydrolysis, periodate oxidation, methylation, and high-resolution 1H and 13C nuclear magnetic resonance studies, the elucidated unambiguous structure was in agreement with our earlier proposal but is at variance with structures proposed later by other authors. The structure of the type 23F pneumococcal polysaccharide is[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F

1984 ◽  
Vol 62 (8) ◽  
pp. 666-677 ◽  
Author(s):  
James C. Richards ◽  
Malcolm B. Perry ◽  
Peter J. Kniskern
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
High Molecular Weight Polymer ◽  
Molecular Weight Polymer ◽  
Magnetic Resonance Studies ◽  
Structure Formula

The specific capsular polysaccharide of Streptococcus pneumoniae type 33F (American type 70) is composed of D-galactose (5 parts), D-glucose (1 part), and O-acetyl (ca. 0.4 parts). Periodate oxidation, partial hydrolysis, and 1H and 13C nuclear magnetic resonance studies showed that the polysaccharide is a high molecular weight polymer of a repeating hexasaccharide unit having the structure:[Formula: see text]

The specific capsular polysaccharide of Streptococcus pneumoniae type 9V

1981 ◽  
Vol 59 (7) ◽  
pp. 524-533 ◽  
Author(s):  
Malcolm B. Perry ◽  
Virginia Daoust ◽  
Dennis J. Carlo
Keyword(s):  
Molecular Weight ◽  
Nuclear Magnetic Resonance ◽  
Streptococcus Pneumoniae ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Optical Rotation ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Part D ◽  
Magnetic Resonance Studies

The specific capsular polysaccharide produced by Streptococcus pneumoniae type 9V (American type 68) is composed of D-glucuronic acid (1 part), D-galactose (1 part), 2-acetamido-2-deoxy-D-mannose (1 part), D-glucose (2 parts), and O-acetyl (1.6 parts). Methylation, periodate oxidation, optical rotation, and nuclear magnetic resonance studies, and partial hydrolysis showed that the polysaccharide is an unbranched high molecular weight linear polymer of a partially O-acetylated pentasaccharide repeating unit having the structure indicated below.[Formula: see text]

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