The Structure of the Capsular Polysaccharide of Klebsiella K-type 72; Occurrence of 3,4-O-(1-Carboxyethylidene)-L-rhamnose

1974 ◽  
Vol 52 (4) ◽  
pp. 684-687 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton
Keyword(s):  
Capsular Polysaccharide ◽  
Partial Hydrolysis ◽  
Unit Formula

Methylation and partial hydrolysis studies on the capsular polysaccharide of Klebsiella K72 show the structure to consist of a repeating unit[Formula: see text]The anomeric linkages were determined in isolated oligosaccharides by p.m.r. spectroscopy which also showed the presence in the polysaccharide of one pyruvate ketal per four sugar residues.

Structure of the Capsular Polysaccharide of Klebsiella K-type 20

1973 ◽  
Vol 51 (18) ◽  
pp. 3015-3020 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton
Keyword(s):  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Acetyl Group ◽  
Unit Formula

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer, of Klebsiella K20 show the structure to consist of a repeating unit[Formula: see text]The anomeric linkages were determined by p.m.r. spectroscopy of the carboxyl reduced polysaccharide, and periodate degraded polysaccharides. The p.m.r. spectroscopy of the original polysaccharide also showed the presence in the polysaccharide of one O-acetyl group per eight sugar residues.

Structure of the capsular polysaccharide antigen of type IV group B Streptococcus

1989 ◽  
Vol 67 (5) ◽  
pp. 877-882 ◽  
Author(s):  
Jose L. Di Fabio ◽  
Francis Michon ◽  
Jean-Robert Brisson ◽  
Harold J. Jennings ◽  
Michael R. Wessels ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Molar Ratio ◽  
Partial Hydrolysis ◽  
Spectroscopic Techniques ◽  
Polysaccharide Antigen ◽  
Type Iv ◽  
High Resolution Nmr ◽  
Group B ◽  
Unit Formula

The native polysaccharide antigen isolated from type IV group B Streptococcus contains D-galactose, D-glucose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in the molar ratio 2:2:1:1 and is composed of the following repeating unit:[Formula: see text]The structural analysis of this antigen was based on results obtained from methylation analysis, partial hydrolysis, nitrous acid deamination, and nuclear magnetic resonance spectroscopic techniques. Keywords: capsular polysaccharide, high resolution NMR spectroscopy, group B Streptococcus, polysaccharide structure, polysaccharide degradation.

The Structure of the Capsular Polysaccharide of Klebsiella K-type 5

1973 ◽  
Vol 51 (11) ◽  
pp. 1826-1832 ◽  
Author(s):  
Guy G. S. Dutton ◽  
Mo-Tai Yang
Keyword(s):  
Molecular Weight ◽  
Capsular Polysaccharide ◽  
Gel Filtration ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Unit Formula

Methylation, periodate oxidation and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer of Klebsiella K5 show the structure to consist of a repeating unit:[Formula: see text]The polysaccharide has a molecular weight (by gel filtration) of 9 × 105 and is the first Klebsiella capsule to be found lacking a carbohydrate side chain. The proportion of O-acetyl and pyruvate groups was determined by p.m.r. spectroscopy.

The Structure of the Capsular Polysaccharide of Klebsiella K-type 24

1973 ◽  
Vol 51 (11) ◽  
pp. 1819-1825 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton ◽  
Alberto M. Zanlungo
Keyword(s):  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Acetyl Group ◽  
Unit Formula

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer, of Klebsiella K24 show the structure to consist of a repeating unit[Formula: see text]The anomeric linkages were determined in isolated oligosaccharides by p.m.r. spectroscopy which also showed the presence in the polysaccharide of one O-acetyl group per seven or eight sugar residues. The O-acetyl is tentatively assigned to one of the D-mannose units.

The Structure of the Capsular Polysaccharide from Klebsiella K-Type 21

1973 ◽  
Vol 51 (2) ◽  
pp. 198-207 ◽  
Author(s):  
Y. M. Choy ◽  
G. G. A. Dutton
Keyword(s):  
Pyruvic Acid ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Partial Hydrolysis ◽  
Methyl Sulfoxide ◽  
Unit Formula

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polymer, of Klebsiella K21 show the structure to consist of a repeating unit.[Formula: see text]The anomeric linkages were determined by p.m.r. spectroscopy of isolated oligosaccharides and, in part, by specific enzymes. P.m.r. spectroscopy of the original polysaccharide in methyl sulfoxide-d6 showed clearly a ratio of one pyruvic acid ketal (CH3, τ 8.5) to five anomeric protons (τ 4.65–5.40).

4,6-O-(1-Carboxyethylidene)-D-mannose as a Structural Unit in Capsular Polysaccharide of Klebsiella K-type 5

1972 ◽  
Vol 50 (14) ◽  
pp. 2382-2384 ◽  
Author(s):  
G. G. S. Dutton ◽  
M. T. Yang
Keyword(s):  
Experimental Data ◽  
Structural Unit ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Acetyl Group

Methylation, periodate oxidation, and partial hydrolysis techniques have each been used to demonstrate the presence of 4,6-O-(1-carboxyethylidene)-D-mannopyranosyl units in the capsular polysaccharide of Klebsiella K-type 5. The structure of this polysaccharide differs from those known for other Klebsiella capsules by the lack of any carbohydrate side chain. A repeating unit of[Formula: see text](plus one unassigned O-acetyl group) is in accord with the experimental data.

Structural elucidation of the capsular polysaccharide of Neisseriameningitidis group H

1984 ◽  
Vol 62 (8) ◽  
pp. 1519-1524 ◽  
Author(s):  
Francis Michon ◽  
René Roy ◽  
Harold J. Jennings ◽  
Fraser E. Ashton
Keyword(s):  
Capsular Polysaccharide ◽  
Specific Antigen ◽  
Structural Elucidation ◽  
Major Group ◽  
Molar Ratio ◽  
Substitution Pattern ◽  
Specific Determinant ◽  
Unit Formula

The capsular polysaccharide from newly isolated Neisseriameningitidis group H elaborates a structurally unique polysaccharide which is the group specific antigen. The polysaccharide contains D-galactose, glycerol, and phosphate in the molar ratio of 1:1:1 and is composed of the following basic repeating unit:[Formula: see text]The polysaccharide contains O-acetyl groups, in the molar ratio of 0.8:1.0 with D-galactose, which are essential to the formation of the major group specific determinant based on serological experiments described. Although all the O-acetyl groups are located on D-galactopyranosyl residues, the substitution pattern is complex, 60% of the residues being substituted at O-3 and 20% at O-2.

scholarly journals THE IMMUNOLOGICAL SPECIFICITY OF TYPE II PNEUMOCOCCUS AND ITS SEPARATION INTO PARTIAL SPECIFICITIES

1956 ◽  
Vol 103 (2) ◽  
pp. 189-197 ◽  
Author(s):  
Michael Heidelberger ◽  
John Adams
Keyword(s):  
Quantitative Data ◽  
Capsular Polysaccharide ◽  
Glucuronic Acid ◽  
Gum Arabic ◽  
Partial Hydrolysis ◽  
Type Ii ◽  
Branch Points ◽  
Tamarind Seed ◽  
Immunological Specificity ◽  
Multiple Units

The specificity of Type II pneumococcus determined by its capsular polysaccharide (S II) may be separated into three partial specificities, each characteristic of one of the three component sugars of S II, namely, glucuronic acid, glucose, and rhamnose. By far the largest portion of antibodies in Type II antipneumococcus horse sera which cross-react with carbohydrates containing one or more of these sugars are reactive with those characterized by multiple groupings of glucuronic acid. This confirms, extends, and explains earlier observations. In confirmation of predictions based upon existing information tamarind seed polysaccharide (jellose), in which much of the glucose exists as 1,4,6-branch points, was found to react in Type II antisera. Several instances are given of cross-reactions in these antisera apparently due to the L-rhamnose component of the reacting polysaccharides. The antisera contain far more antibody capable of precipitating with substances with multiple units of glucuronic acid than with those so far tested containing multiple 1,4,6-branch points of glucose or multiple groupings of rhamnose. The long known increase of titer of gum arabic on partial hydrolysis is now fully explained and discussed, and a chemical picture is given of the change in "avidity." The sum of the partial specificities measured does not equal the whole. Quantitative data illustrating these points are given.

Structure of the Capsular Polysaccharide of Klebsiella K-type 56

1973 ◽  
Vol 51 (18) ◽  
pp. 3021-3026 ◽  
Author(s):  
Yuen-Min Choy ◽  
Guy G. S. Dutton
Keyword(s):  
Uronic Acid ◽  
Capsular Polysaccharide ◽  
Periodate Oxidation ◽  
Side Chain ◽  
Partial Hydrolysis ◽  
Hydrolysis Of

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide of Klebsiella K-type 56 show the structure to be a repeating unit consisting of[Formula: see text]The nature of the anomeric linkages was determined by p.m.r. spectroscopy of isolated oligosaccharides. The position of the L-rhamnose side chain was defined by characterization of the di- and tetrasaccharides obtained by partial hydrolysis of the fully methylated polysaccharide.This structure represents the first capsular polysaccharide lacking uronic acid to be studied in the genus Klebsiella.

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