Structure of the capsular polysaccharide antigen of type IV group B Streptococcus

1989 ◽  
Vol 67 (5) ◽  
pp. 877-882 ◽  
Author(s):  
Jose L. Di Fabio ◽  
Francis Michon ◽  
Jean-Robert Brisson ◽  
Harold J. Jennings ◽  
Michael R. Wessels ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Molar Ratio ◽  
Partial Hydrolysis ◽  
Spectroscopic Techniques ◽  
Polysaccharide Antigen ◽  
Type Iv ◽  
High Resolution Nmr ◽  
Group B ◽  
Unit Formula

The native polysaccharide antigen isolated from type IV group B Streptococcus contains D-galactose, D-glucose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in the molar ratio 2:2:1:1 and is composed of the following repeating unit:[Formula: see text]The structural analysis of this antigen was based on results obtained from methylation analysis, partial hydrolysis, nitrous acid deamination, and nuclear magnetic resonance spectroscopic techniques. Keywords: capsular polysaccharide, high resolution NMR spectroscopy, group B Streptococcus, polysaccharide structure, polysaccharide degradation.

Structural determination and serology of the native polysaccharide antigen of type-III group B Streptococcus

1980 ◽  
Vol 58 (2) ◽  
pp. 112-120 ◽  
Author(s):  
Harold J. Jennings ◽  
Karl-Gunnar Rosell ◽  
Dennis L. Kasper
Keyword(s):  
Sialic Acid ◽  
Group B Streptococcus ◽  
Rabbit Antiserum ◽  
Ph Control ◽  
Molar Ratio ◽  
Polysaccharide Antigen ◽  
Type Iii ◽  
Group B ◽  
Unit Formula ◽  
Ph Controlled

The native polysaccharide antigen isolated from type-III group B Streptococcus contains D-galactose (Gal), D-glucose (Glc), 2-acetamido-2-deoxy-D-glucose (GlcNAc), and sialic acid (NeuNAc) in the molar ratio of 2:1:1:1 and its structure can be represented by the following repeating unit:[Formula: see text]By cleavage of all the labile sialic acid end groups the incomplete type-III antigen is obtained which is structurally identical to the S. pneumoniae type-14 polysaccharide. Thus, the native type-III polysaccharide is serologically distinct from the incomplete type-III antigen by virtue of the former having determinants terminating in sialic acid and the latter in β-D-galactopyranose units. None of these latter determinants could be detected in streptococcal organisms grown under pH-controlled conditions (pH 7.0) or in rabbit antiserum made to these pH-controlled organisms. However, in antisera produced in rabbits to the same organisms grown without pH control (Lancefield procedures), antibodies to both types of determinant could be detected. This can be attributed to the removal of some of the masking sialic acid residues from the cell-associated native polysaccharide by degradative procedures which occur during these latter conditions.

Structural elucidation of the novel type VII group B Streptococcus capsular polysaccharide by high resolution NMR spectroscopy

1995 ◽  
Vol 277 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Grigorij Kogan ◽  
Jean-Robert Brisson ◽  
Dennis L. Kasper ◽  
Christina von Hunolstein ◽  
Graziella Orefici ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
High Resolution ◽  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Structural Elucidation ◽  
The Novel ◽  
High Resolution Nmr ◽  
Group B

scholarly journals Glucuronoxylomannan, the Major Capsular Polysaccharide of Cryptococcus neoformans, Inhibits the Progression of Group B Streptococcal Arthritis

2004 ◽  
Vol 72 (11) ◽  
pp. 6367-6372 ◽  
Author(s):  
Luciana Tissi ◽  
Manuela Puliti ◽  
Francesco Bistoni ◽  
Paolo Mosci ◽  
Thomas R. Kozel ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Cryptococcus Neoformans ◽  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Peritoneal Macrophages ◽  
Type Iv ◽  
Inflammatory Protein ◽  
Group B

ABSTRACT Glucuronoxylomannan (GXM), the principal constituent of the Cryptococcus neoformans capsule, modulates the inflammatory response of human monocytes in vitro. Here we examine the efficacy of GXM as a novel anti-inflammatory compound for use against experimental septic arthritis. Arthritis was induced in mice by the intravenous injection of 8 × 106 CFU of type IV group B streptococcus (GBS). GXM was administered intravenously in different doses (50, 100, or 200 μg/mouse) 1 day before and 1 day after bacterial inoculation. GXM treatment markedly decreased the incidence and severity of articular lesions. Histological findings showed limited periarticular inflammation in the joints of GXM-treated mice, confirming the clinical observations. The amelioration of arthritis was associated with a significant reduction in the local production of interleukin-6 (IL-6), IL-1β, macrophage inflammatory protein 1α (MIP-1α), and MIP-2 and an increase in systemic IL-10 levels. Moreover, peritoneal macrophages derived from GXM-treated mice and stimulated in vitro with heat-inactivated GBS showed a similar pattern of cytokine production. The present study provides evidence for the modulation of the inflammatory response by GXM in vivo and suggests a potential therapeutic use for this compound in pathologies involving inflammatory processes.

scholarly journals Isolation of type-specific polysaccharide antigen from group B type Ib streptococci.

1979 ◽  
Vol 149 (1) ◽  
pp. 58-66 ◽  
Author(s):  
J Y Tai ◽  
E C Gotschlich ◽  
R C Lancefield
Keyword(s):  
Sialic Acid ◽  
Group B Streptococcus ◽  
Molar Ratio ◽  
Polysaccharide Antigen ◽  
Absorption Studies ◽  
Type Ia ◽  
Specific Polysaccharide ◽  
Group B ◽  
Sepharose 4B ◽  
Specific Determinant

Group B streptococcus type Ib (strain H36B) was subjected to digestion with extracellular muralytic enzymes prepared from Streptomyces albus. Type Ib-specific polysaccharide antigen was isolated from the lysate by alcohol precipitation and Sepharose 6B chromatography. The purified type Ib antigen has a Kd value of 0.31 on a Sepharose 4B column and contains four sugars, galactose, glucose, N-acetyl glucosamine, and sialic acid in a molar ratio of 2.05:0.86:1.00:0.90. Acid treatment (pH 2.0) of this polysaccharide results in partial degradation of the antigen (Kd = 0.41 on Sepharose 4B) with the loss of 93% of the sialic acid. The molar ratio of the remaining sugars in the polysaccharide remains identical to that in the native one. This suggests that the sialic acid is at the terminal position in the molecule. Both intact and acid-treated antigen cross-react with some type Ia and type Ic antisera as a result of the common Iabc determinant, but not with type II and type III antisera. Absorption studies indicate that Ib-specific determinant and Iabc determinant are on the same molecule and that sialic acid is not the cross-reactive determinant.

scholarly journals Isolation and characterization of type IV group B Streptococcus capsular polysaccharide.

1989 ◽  
Vol 57 (4) ◽  
pp. 1089-1094 ◽  
Author(s):  
M R Wessels ◽  
W J Benedí ◽  
H J Jennings ◽  
F Michon ◽  
J L DiFabio ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
Group B Streptococcus ◽  
Type Iv ◽  
Isolation And Characterization ◽  
Group B

Group B Streptococcal and Pneumococcal Sepsis in Newborn Infants: The Role of Pneumococcal Antibody

PEDIATRICS ◽  
1978 ◽  
Vol 62 (4) ◽  
pp. 620-621
Author(s):  
Gerald W. Fischer ◽  
James W. Bass ◽  
George H. Lowell ◽  
Martin H. Crumrine
Keyword(s):  
Streptococcus Pneumoniae ◽  
Hydrochloric Acid ◽  
Group B Streptococcus ◽  
Newborn Infants ◽  
Polysaccharide Antigen ◽  
Type Iii ◽  
Pneumococcal Sepsis ◽  
Group B

The article by Bortolussi et al. on pneumococcal septicemia and meningitis in the neonat (Pediatrics 60:352, September 1977) was of great interest to us, since we have been analyzing the effect of antibody directed against Streptococcus pneumoniae on group B Streptococcus type III. We have recently shown (unpublished data) that antibody directed against S. pneumoniae type 14 precipitates the hot hydrochloric acid-extracted polysaccharide antigen of group B Streptococcus type III. Further studies have shown that this antibody is opsonic for group B Streptococcus type III in an in vitro bactericidal assay and protective in a suckling rat model of group B Streptococcus type III sepsis.1

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