Utility of a pre-optimized kit for random amplified polymorphic DNA in typingCandida albicans

2002 ◽  
Vol 48 (4) ◽  
pp. 369-373 ◽  
Author(s):  
Kathleen M Riederer ◽  
Jambunathan Ramanathan ◽  
Jeff Barczak ◽  
Joseph Baran, Jr. ◽  
Riad Khatib
Keyword(s):  
Candida Albicans ◽  
Genomic Dna ◽  
Reliable Method ◽  
Random Amplified Polymorphic Dna ◽  
Turnaround Time ◽  
Genetic Variations ◽  
Ease Of Use ◽  
Stool Samples ◽  
Quick Turnaround ◽  
Quick Turnaround Time

The utility of a pre-optimized kit for random amplified polymorphic DNA (RAPD) was assessed in typing diverse strains of Candida albicans from epidemiologically unrelated inpatients (interpatient analysis) and in detecting clonal variations that maybe present within individual patient isolates (intrapatient analysis). Stool samples from inpatients were cultured on Inhibitory Mold agar. Nine individual colonies from all patients with [Formula: see text]9 colonies of C. albicans (n = 18) were selected, frozen, and karyotyped using CHEF genomic DNA plug kits and CHEF-DRIII. Each of the selected colonies was then analyzed by RAPD, utilizing the selected kit, with 6 primers. Interpatient analysis revealed 9 karyotypes and 17 RAPD composites. RAPD discrimination was significantly better (p < 0.001). Intrapatient analysis revealed 34 (21%) and 33 (20.4%) variants among 162 colonies tested by RAPD and karyotyping, respectively. The results were discordant in 25 variants, all with differences of 1–3 bands. These results illustrate that this pre-optimized kit for RAPD provides excellent discrimination of genetically unrelated strains. Its performance in delineating subtle clonal differences was comparable with karyotyping; both methods failed to detect all minor genetic variations. The ease of use and quick turnaround time of this kit offer a practical and reliable method for typing diverse strains of C. albicans, but may be inadequate for assessing microevolution.Key words: Candida albicans, karyotyping, RAPD.

scholarly journals Supplier evaluation in industrial power services: a case study in gas-turbine maintenance, repair, and overhaul

2020 ◽  
Vol 202 ◽  
pp. 13002
Author(s):  
Yordian Fachrie ◽  
Arviansyah
Keyword(s):  
Gas Turbine ◽  
Low Cost ◽  
Skill Training ◽  
Operating Cost ◽  
Turnaround Time ◽  
Turbine Engine ◽  
Quick Turnaround ◽  
The Right ◽  
Industrial Gas Turbine ◽  
Quick Turnaround Time

The maintenance is one of the highest costs in a gas-turbine engine, after operating cost with approximately about 14-19 % of the total cost. Some of the operators do not have spare engines, and it will lead to operation shutdown. With the current market, most MRO challenged to provide their costumer to achieve quick turnaround time (TAT) at a low cost without affecting the quality of the product. Since MRO is selling the skill services, it took applied technology, skill training, and experience to deliver quality, which needs high cost. Therefore, MRO needs to collaborate with other parties (original manufacturer or others) to increase its capacity and capability. MRO should concern more for evaluating the vendors to align with the strategies to get quick turnaround time with the right quality product. Supplier selection is the objective of this research by analyzing the selection criteria at Industrial Gas-Turbine maintenance. The highest priority is the vendor effectiveness followed by the quality, cost, risk management. The highest weight is based on the priority of the supplier.

Enabling Cell Aware Diagnosis in a Foundry for Accurate and Efficient Failure Analysis of Cell Internal Defects

2020 ◽  
Author(s):  
Rohan Deshpande ◽  
Gregory Billus ◽  
Nikitha Penmethsa ◽  
Davide Pacifico ◽  
Huaxing Tang ◽  
...  
Keyword(s):  
Failure Analysis ◽  
Turnaround Time ◽  
Internal Layer ◽  
Standard Cell ◽  
Internal Defects ◽  
Standard Cells ◽  
Quick Turnaround ◽  
Random Defects ◽  
Quick Turnaround Time

Abstract Cell aware diagnosis identifies defects within the standard cell as opposed to traditional layout aware diagnosis that identifies the failing standard cell or the area between two standard cells. In a mature technology dominated by random defects, cell aware results pinpoint the cell internal layer drastically reducing the turnaround time for failure analysis. This paper describes a method to enable cell aware diagnosis in a foundry environment, perform a volume diagnosis analysis with RCAD (fail mode pareto) and drive failure analysis with a quick turnaround time for a 14nm customer chip.

Simple and quick turnaround time fabrication process for deep submicrometer CMOS generation

1996 ◽  
Vol 9 (4) ◽  
pp. 489-494 ◽  
Author(s):  
H. Koike ◽  
F. Matsuoka ◽  
H. Ohtsuka ◽  
M. Kakumu
Keyword(s):  
Turnaround Time ◽  
Fabrication Process ◽  
Deep Submicrometer ◽  
Quick Turnaround ◽  
Quick Turnaround Time

Quick-turnaround-time improvement for product development and transfer to mass production

1998 ◽  
Vol 11 (1) ◽  
pp. 54-62
Author(s):  
H. Koike ◽  
F. Matsuoka ◽  
S. Hohkibara ◽  
E. Fukuda ◽  
K. Tomioka ◽  
...  
Keyword(s):  
Product Development ◽  
Mass Production ◽  
Turnaround Time ◽  
Quick Turnaround ◽  
Quick Turnaround Time

Evaluation of the first immunosuppressive drug assay available on a fully automated LC-MS/MS-based clinical analyzer suggests a new era in laboratory medicine

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Sebastian Hörber ◽  
Andreas Peter ◽  
Rainer Lehmann ◽  
Miriam Hoene
Keyword(s):  
High Specificity ◽  
Turnaround Time ◽  
Immunosuppressive Drugs ◽  
Immunosuppressive Drug ◽  
Ease Of Use ◽  
Analytical Performance ◽  
University Hospital ◽  
Therapeutic Monitoring ◽  
Mean Deviation ◽  
Liquid Chromatography Tandem Mass

AbstractObjectivesDue to its high specificity, liquid chromatography-tandem mass spectrometry (LC-MS/MS) is considered the gold standard in diagnostic areas such as therapeutic monitoring of immunosuppressive drugs (ISDs). However, many laboratories still rely on immunoassays for ISD quantification in a tradeoff between analytical performance and the advantages of fully automated analyzers – shorter turnaround times, greater ease of use, and 24/7 availability.MethodsThe LC-MS/MS-based Thermo Scientific™ Cascadion™ SM Immunosuppressant Panel was evaluated for >6 months in the routine laboratory of a university hospital. We assessed the analytical performance of the panel and compared it to conventional LC-MS/MS as well as to immunoassays (cyclosporine A, sirolimus, tacrolimus (Siemens) and everolimus (Thermo Fisher)). In addition, both ISD panel and Cascadion analyzer were scrutinized with regards to, e.g., turnaround time, usability, and robustness.ResultsAll ISDs showed high linearity and precision (CV≤6%) and a good correlation with conventional LC-MS/MS. The mean deviation to the immunoassays was 17–19% and negative for all ISDs except everolimus with a positive 19% bias. No weak points were revealed when challenging assay and system with, e.g., high haematocrit, sedimented whole blood or priority samples. The Cascadion integrated well into our 24/7 routine and could easily be operated simultaneously with several other analyzers by technical staff without LC-MS experience.ConclusionsThe ISD panel showed excellent analytical performance and demonstrated that a fully automated LC-MS-based analysis starting from primary samples is feasible, suggesting that LC-MS could become an integral part of 24/7 diagnostics in the near future.

scholarly journals Genetic Diversity of Salvia Species Assessed by ISSR and RAPD Markers

2016 ◽  
Vol 44 (2) ◽  
pp. 431-436 ◽  
Author(s):  
Masoumeh YOUSEFIAZARKHANIAN ◽  
Ali ASGHARI ◽  
Jafar AHMADI ◽  
Behvar ASGHARI ◽  
Ali Ashraf JAFARI
Keyword(s):  
Genetic Diversity ◽  
Genetic Polymorphisms ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Genetic Variations ◽  
Molecular Techniques ◽  
Wild Populations ◽  
Similarity Coefficients ◽  
The World

The genus Salvia includes an enormous assemblage of nearly 1,000 species dispersed around the world. Due to possible threats to this genus, there is an immediate requirement to evaluate the diversity of its wild populations. ISSR and RAPD molecular techniques were used to evaluate the genetic relationships among twenty-one ecotypes of eight Salvia species. Amplification of genomic DNA using 23 primers (15 RAPD and eight ISSR) produced 280 bands, of which 91% were polymorphic. The results of marker parameters showed no clear difference between two marker systems. It was generally observed that both ISSR and RAPD markers had similar efficiency in detecting genetic polymorphisms with remarkable ability to differentiate the closely related ecotypes of Salvia. Nei’s similarity coefficients for these techniques ranged from 0.48 to 0.98. Based on the results of clustering, PCoA and AMOVA, the genetic diversity between and within species was confirmed. So, conservation and domestication of the genus Salvia must be due to levels of genetic variations.

scholarly journals Use of random amplified polymorphic DNA as a typing method for Candida albicans in epidemiological surveillance of a burn unit.

1995 ◽  
Vol 33 (9) ◽  
pp. 2366-2371 ◽  
Author(s):  
F Robert ◽  
F Lebreton ◽  
M E Bougnoux ◽  
A Paugam ◽  
D Wassermann ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Random Amplified Polymorphic Dna ◽  
Epidemiological Surveillance ◽  
Typing Method ◽  
Burn Unit

Direct detection ofCampylobacter jejuniin human stool samples by real-time PCR

2008 ◽  
Vol 54 (9) ◽  
pp. 742-747 ◽  
Author(s):  
Shiyong Lin ◽  
Xinying Wang ◽  
Haoxuan Zheng ◽  
Zhengguo Mao ◽  
Yong Sun ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Direct Detection ◽  
Culture Method ◽  
Turnaround Time ◽  
Culture Methods ◽  
Time Saving ◽  
Whole Process ◽  
Pure Cultures ◽  
Stool Samples

Our purpose was to establish a quick and accurate real-time PCR (rtPCR) method to detect Campylobacter jejuni directly from human diarrheal stool as an alternative to traditional culture methods. To determine the consistency of rtPCR and culture method, 256 clinical diarrheal stool samples and 50 normal stool samples from healthy individuals were examined, and the whole process was double-blinded. Our data showed that the sensitivity of rtPCR in pure cultures and stool was 102CFU·mL–1and 103CFU·g–1, respectively. Of the 256 diarrheal samples, 10 specimens were successfully detected by both methods, whereas two specimens were PCR positive but culture negative. No positive results were found by these two methods in 50 normal specimens. Our data suggested that rtPCR was convenient in operation and time-saving (turnaround time 3.5–4 h), so it could be used for clinical diagnostic and epidemiological purposes.

RAPD and Protein Analyses Revealed Polymorphism in Mutated Potato Cultivars

2013 ◽  
Vol 64 (2) ◽  
Author(s):  
Irshad Ahmad ◽  
Samiullah Khan ◽  
Muhammad Arshad Javed ◽  
Fahrul Zaman Huyop ◽  
Muhammad Tariq ◽  
...  
Keyword(s):  
Banding Pattern ◽  
Total Protein ◽  
Genomic Dna ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Potato Cultivars ◽  
Polymorphic Behavior ◽  
Mutant Lines

Genomic DNA of the mutant lines of the three potato cultivars, Cardinal, Diamant and Desiree, with respect to controls were isolated and analyzed for polymorphisms by using random amplified polymorphic DNA (RAPD) markers. Four 10 bp random fragment primers, S-13, S-18, S-19 and R-17 were studied and all of them gave the amplification of genomic DNA. All of the mutant lines gave different banding pattern against different primers with respect to control plants of the three varieties, and bands are present at 50 bp to 1500 bp. All these primers with specific banding pattern were unique in their polymorphic behavior. Different banding pattern of total protein contents were also observed by PAGE analysis of all the mutant lines as compared with the control plants. It is therefore suggested that RAPD and protein analyses would be important tools to detect the polymorphism in mutated lines of potato.

Evaluation of two point of care technologies for measuring monoclonal antibody therapeutic-A concentrations in blood

Bioanalysis ◽  
2020 ◽  
Vol 12 (20) ◽  
pp. 1449-1458
Author(s):  
Saloumeh K Fischer ◽  
Kathi Williams ◽  
Ian Harmon ◽  
Bryan Bothwell ◽  
Hua Xu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Real Time ◽  
Point Of Care ◽  
Turnaround Time ◽  
Monitoring Methods ◽  
Sample Collection ◽  
Time Data ◽  
Serum Samples ◽  
Quick Turnaround Time

Aim: Current blood monitoring methods require sample collection and testing at a central lab, which can take days. Point of care (POC) devices with quick turnaround time can provide an alternative with faster results, allowing for real-time data leading to better treatment decisions for patients. Results/Methodology: An assay to measure monoclonal antibody therapeutic-A was developed on two POC devices. Data generated using 75 serum samples (65 clinical & ten spiked samples) show correlative results to the data generated using Gyrolab technology. Conclusion: This case study uses a monoclonal antibody therapeutic-A concentration assay as an example to demonstrate the potential of POC technologies as a viable alternative to central lab testing with quick results allowing for real-time decision-making.

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