Tannic acid induces transcription of laccase genecglcc1in the white-rot fungusCoriolopsis gallica

2002 ◽  
Vol 48 (12) ◽  
pp. 1041-1047 ◽  
Author(s):  
José M Carbajo ◽  
Howard Junca ◽  
María C Terrón ◽  
Tania González ◽  
Susana Yagüe ◽  
...  
Keyword(s):  
Tannic Acid ◽  
Laccase Activity ◽  
Culture Media ◽  
Environmental Pollutants ◽  
White Rot Fungi ◽  
Extracellular Enzyme ◽  
White Rot ◽  
Laccase Gene ◽  
Extracellular Laccase

Laccase, a phenoloxidase enzyme secreted by white-rot fungi, has a significant role in the degradation of lignin and environmental pollutants. Coriolopsis gallica is a ligninolytic basidiomycete that produces high levels of this extracellular enzyme. A laccase gene cglcc1 from this fungus has been cloned and sequenced. The capacity of C. gallica to efficiently degrade polyphenols has been successfully applied in our laboratory to the biotreatment and decolorization of several industrial wastewaters. This study focused on the effect of tannic acid, a natural compound widely distributed in plants, on the production of laccase activity by C. gallica. Our results showed an evident increase of extracellular laccase levels when C. gallica was grown in the presence of tannic acid. Concentrations of 50 and 100 μM of this compound increased laccase activity when compared with control samples grown without tannic acid. In addition, we found an increase in laccase transcript levels in C. gallica grown in culture media supplemented with tannic acid. The role of tannic acid was shown to be an inductor of laccase activity in this fungus, due to the enhancement of expression of the laccase gene at the transcriptional level.Key words: laccase, tannic acid, Coriolopsis gallica, induction, gene transcription.

Biological pretreatment of rice husks with the white-rot fungus pleurotus ostreatus: the role of laccase activity

2021 ◽  
Author(s):  
◽  
Dinary Durán Sequeda
Keyword(s):  
Laccase Activity ◽  
Culture Media ◽  
White Rot ◽  
White Rot Fungus ◽  
Rice Husks ◽  
Media Composition ◽  
Biological Pretreatment ◽  
Submerged Cultures ◽  
Nutritional Conditions

The main objective of this study was to determine the influence of the composition of the culture medium and lignocellulosic compounds on the secretion of laccase enzymes by P. ostreatus in submerged cultures. These studies were done using a statistical and systematic approach that allowed the control of the culture media composition. The optimal nutritional conditions were found that simultaneously increased fungal growth and laccase activity in the absence and presence of copper sulfate, a recognized inducer of laccase. Under these conditions, the biochemical aspects of transcripts in P. ostreatus related to laccase secretion were evaluated , which revealed the participation of membrane transporters with high affinity for copper (CTRs) as intermediate candidates for the regulation of three laccase genes, lacc2, lacc6, and lacc10. Moreover, the evaluation of the results of the culture media composition suggests that the regulation of these transporters is closely linked to sufficient nutritional conditions in carbon and nitrogen, with central participation of the metabolism of organic nitrogen in this process. With these findings, it was possible to obtain more profound knowledge of the pretreatment of lignocellulosic biomass by P. ostreatus in a submerged culture that was oriented to determine the role of laccase activity in the biological pretreatment of rice husks.

Potential role of Bacillus endospores in soil amended by olive mill wastewater

2010 ◽  
Vol 61 (11) ◽  
pp. 2873-2879 ◽  
Author(s):  
Gino Naclerio ◽  
Antonio Falasca ◽  
Emma Petrella ◽  
Valentina Nerone ◽  
Federica Cocco ◽  
...  
Keyword(s):  
Potential Role ◽  
Laccase Activity ◽  
White Rot Fungi ◽  
Olive Mill Wastewater ◽  
White Rot ◽  
Bacillus Species ◽  
Italian Law ◽  
Bacillus Spores ◽  
Olive Mill

The main aim of this work was to know how spread is laccase activity in spores of Bacillus species isolated from a soil where Italian law allows olive mill wastewater (OMW) spreading, and to investigate the potential role of such autochthonous soil microorganisms in degradation of OMW phenols, and prevention of groundwater pollution. Laccase activity was detected for the first time in spores of wild-type Bacilluspumilus, B. cereus sensu lato, and B. amyloliquefaciens strains. Because B. pumilus, B. cereus sensu lato, and B. amyloliquefaciens, together with B. subtilis account for a total of 93% of Bacillus isolates at the study site, the nearly totality of Bacillus spores reveals laccase activity. Thus, taking also into consideration that Bacillus spores are more abundant (about 100-fold) than white-rot fungi (that possess a well known extracellular, radical-based ligninolytic enzyme system capable of degrading OMW phenols) in the studied soil, these spores may contribute to in-situ degradation of OMW phenols. This role is further emphasized by dilution of crude OMW during infiltration of rainwater through soil that allows to minimize the antibacterial activity of phenols. The widespread presence of Bacillus spores in soils indicates a potential detoxifying role of these spores in a broader context.

scholarly journals The attraction of Tremex apicalis (Hymenoptera, Siricidae, Tremecinae) and its parasitoid Ibalia japonica (Hymenoptera, Ibaliidae) to the fungus Cerrena unicolor

2019 ◽  
Vol 68 ◽  
pp. 37-48 ◽  
Author(s):  
Kazumu Kuramitsu ◽  
Teruhito Ishihara ◽  
Aki Sugita ◽  
Thitaree Yooboon ◽  
Barry Lustig ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Dna Barcode ◽  
White Rot Fungi ◽  
White Rot ◽  
Searching Behavior ◽  
Host Searching ◽  
Additional Species ◽  
Saproxylic Insects ◽  
Symbiotic Fungi

Woodwasps (Hymenoptera: Siricidae) are saproxylic insects and a common forest pest. Siricid woodwasps are classified into two subfamilies: Siricinae and Tremecinae. All known symbiotic fungi of Siricinae are in the genusAmylostereumBoidin while some species of Tremecinae have been observed to have a relationship with the fungusCerrenaunicolor(Bull.) Murrill. Previous studies about the host searching behavior of woodwasps and their parasitoids have focused primarily on the subfamily Siricinae.We analyzed the role ofC.unicolorvolatiles on the host searching behavior ofTremexapicalisMatsumura (Hymenoptera: Siricidae: Tremecinae) and its parasitoid Ibalia (Tremibalia) japonica Matsumura (Hymenoptera: Ibaliidae). The results of an olfactory response experiment indicated that the females ofT.apicalisand its parasitoid find their respective hosts using volatiles fromC.unicolor. Using DNA barcode, we identified basidiocarps on the trees infested withT.apicalis.The basidiocarps were all white-rot fungi that cause sapwood decay, includingC.unicolor. Two additional species that we identified belonged to genera closely related toC.unicolor.Woodwasp species are known to carry symbiotic fungi in a pair of specialized sacs called mycangia. Notably we found that mycangia-like structures were absent in the abdomens ofT.apicalisfemales. To the best of our knowledge,Xerisspectrum(Linnaeus) (Hymenoptera: Siricidae) is the only reported example of woodwasp species that do not contain symbiotic fungi in their bodies.Our results suggested that: (1)T.apicalisfemales search for host wood that is already infected with sapwood decaying fungus using volatile compounds; (2)T.apicalis’ female parasitoid also uses volatile compounds from fungus to locate wood that is infested with its potential host.

Enhanced Formation of Extracellular Laccase Activity by the White-Rot Fungus Trametes multicolor

2002 ◽  
pp. 229-241 ◽  
Author(s):  
Johann Hess ◽  
Christian Leitner ◽  
Christiane Galhaup ◽  
Klaus D. Kulbe ◽  
Barbara Hinterstoisser ◽  
...  
Keyword(s):  
Laccase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Extracellular Laccase ◽  
Trametes Multicolor

scholarly journals Use of Multiplex Reverse Transcription-PCR To Study the Expression of a Laccase Gene Family in a Basidiomycetous Fungus

2003 ◽  
Vol 69 (12) ◽  
pp. 7083-7090 ◽  
Author(s):  
Tania González ◽  
María C. Terrón ◽  
Ernesto J. Zapico ◽  
Alejandro Téllez ◽  
Susana Yagüe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reverse Transcription ◽  
White Rot Fungi ◽  
Gene Families ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Laccase Gene ◽  
Reverse Transcription Pcr ◽  
Basidiomycetous Fungus ◽  
Laccase Genes

ABSTRACT Laccases produced by white rot fungi are involved in the degradation of lignin and a broad diversity of other natural and synthetic molecules, having a great potential for biotechnological applications. They are frequently encoded by gene families, as in the basidiomycete Trametes sp. strain I-62, from which the lcc1, lcc2, and lcc3 laccase genes have been cloned and sequenced. A multiplex reverse transcription-PCR method to simultaneously study the expression of these genes was developed in this study. The assay proved to be quick, simple, highly sensitive, and reproducible and is particularly valuable when numerous samples are to be analyzed and/or if the amount of initial mRNA is limited. It was used to analyze the effect of 3,4-dimethoxybenzyl alcohol (veratryl alcohol) and two of its isomers (2,5-dimethoxybenzyl alcohol and 3,5-dimethoxybenzyl alcohol) on differential laccase gene expression in Trametes sp. strain I-62. These aromatic compounds produced different induction patterns despite their chemical similarity. We found 2,5-dimethoxybenzyl alcohol to be the best inducer of laccase activity while also producing the highest increase in gene expression; 3,5-dimethoxybenzyl alcohol was the next best inducer. Transcript amounts of each gene fluctuated dramatically in the presence of these three inducers, while the total amounts of laccase mRNAs seemed to be modulated by a coordinated regulation of the different genes.

scholarly journals Role of ligninolytic enzymes of white rot fungi (Pleurotus spp.) grown with azo dyes

SpringerPlus ◽  
2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Prashant D. Kunjadia ◽  
Gaurav V. Sanghvi ◽  
Anju P. Kunjadia ◽  
Pratap N. Mukhopadhyay ◽  
Gaurav S. Dave
Keyword(s):  
Azo Dyes ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot

scholarly journals Produksi dan karakterisasi lakase Omphalina sp. Production and characterization of Omphalina sp. laccase

2016 ◽  
Vol 75 (2) ◽  
Author(s):  
. SISWANTO ◽  
. SUHARYANTO ◽  
Rossy FITRIA
Keyword(s):  
Oil Palm ◽  
Laccase Activity ◽  
Research Result ◽  
White Rot Fungi ◽  
White Rot ◽  
Partial Purification ◽  
Empty Fruit Bunches ◽  
Optimum Ph ◽  
Laccase Enzyme

SummaryOmphalina sp. a white-rot fungi (WRF)originated from oil palm plantation has abilityto degrade empty fruit bunches of oil palm(EFBOP) so that it is expected to producelaccase with high activity. The ability ofOmphalina sp. to produce laccase enzyme onliquid fermentation will be studied. The enzymewill also be partially purified andcharacterized. The research result showed thatthe highest enzyme activity (1.162 U/mL) wasobtained using glucose malt yeast (GMY)medium at room temperature for four days.The addition of 2,5-xylidine as an inducerproduced laccase earlier i.e two days, but theactivity of laccase was less active afterprolonged incubation compared to that ofcontrol. The laccase produced on mediumcontaining 2% EFBOP reached optimumactivity as much as 0.38 U/mL after 10 th daysof incubation. Partial purification of laccaseon Sephacryl S-200 HR column resulted58.23% of yield recovery with twice purity thanbefore. The optimum pH of laccase was 4.5.Laccase activity was stable even after heatedon 50 o C for 30 minutes, but then decreasedwhen heated until 60 o C. The laccase has K Mand V max as much as 0.15 mM and 0.56 U/mLrespectively.RingkasanOmphalina sp., adalah fungi pelapuk putih(FPP) hasil isolasi dari kebun kelapa sawityang diketahui mampu mendegradasi tandankosong kelapa sawit (TKKS) dengan cepatsehingga diharapkan mampu menghasilkanlakase dengan aktivitas tinggi. KemampuanOmphalina sp. menghasilkan enzim lakasepada fermentasi cair akan dipelajari. Selain itu,lakase yang dihasilkan akan dimurnikan secaraparsial serta dilakukan karakterisasi pH, suhu,dan konsentrasi substrat optimum. Hasilpenelitian menunjukkan bahwa Omphalina sp.menghasilkan lakase dengan aktivitas tertinggi(1,162 U/mL) pada medium glucose malt yeast(GMY) yang diinkubasikan pada suhu ruangselama empat hari. Penambahan 2,5-xilidinsebagai induser mempercepat produksi lakaselebih awal yaitu dalam waktu dua hari, namunaktivitasnya masih lebih rendah dibandingkandengan kontrol pada inkubasi lebih lanjut.Lakase dari Omphalina sp. juga dapatdiproduksi pada medium yang mengandung2% TKKS dan aktivitasnya mencapai0,38 U/mL yang diinkubasi dalam suhu ruangselama 10 hari. Pemurnian parsial pada kolomSephacryl S-200 HR menghasilkan rendemensebesar 58,23% dengan kemurnian dua kalinya.Aktivitas lakase optimum pada pH 4,5 dantetap stabil setelah pemanasan selama 30 menitpada suhu ruang hingga 50 o C dan menuruntajam pada suhu 60 o C. Lakase Omphalina sp.menghasilkan nilai K M dan V maks masing-masing sebesar 0,15 mM dan 0,56 U/mL.

scholarly journals Comparative study on laccase activity of white rot fungi under submerged fermentation with different lignocellulosic wastes

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 9166-9179
Author(s):  
Qi An ◽  
Jie Qiao ◽  
Lu-Sen Bian ◽  
Mei-Ling Han ◽  
Xun-You Yan ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Laccase Activity ◽  
White Rot Fungi ◽  
Industrial Applications ◽  
Culture Conditions ◽  
White Rot ◽  
Laccase Production ◽  
Poplar Wood ◽  
Lignocellulosic Wastes ◽  
Cottonseed Hull

Different Pleurotus ostreatus and Flammulina velutipes species were compared relative to their ability to produce laccase in submerged fermentation of various lignocellulosic wastes. Fungi cultivation in identical culture conditions revealed wide differences among both species and strains of the same species. The laccase secretion ability of P. ostreatus strains was superior to F. velutipes strains. Maximum laccase production on cottonseed hull, corncob, and poplar wood was secreted by P. ostreatus CY 568, P. ostreatus CCEF 89, and P. ostreatus CY 568, respectively. The nature of lignocellulosic materials played an important role in determining the expression of laccase potential of fungi. The presence of cottonseed hull improved laccase activity and accelerated the rate of enzyme production. Maximum laccase production on cottonseed hull was nearly 1.29-fold and 1.53-fold higher than that on corncob and poplar wood, respectively. Laccase activity was detected in almost all tested strains on cottonseed hull on the first day, while only a few strains on poplar wood and corncob were detected on the first day. These findings will be helpful for selecting the appropriate strain in industrial applications and for optimization of integrated industrial laccase production.

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