scholarly journals Characterization, regulation, and phylogenetic analyses of thePenicillium griseoroseumnitrate reductase gene and its use as selection marker for homologous transformation

2004 ◽  
Vol 50 (11) ◽  
pp. 891-900 ◽  
Author(s):  
Jorge Fernando Pereira ◽  
Marisa Vieira de Queiroz ◽  
Francis Júlio Fagundes Lopes ◽  
Rodrigo Barros Rocha ◽  
Marie-Josée Daboussi ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Nitrogen Metabolism ◽  
Phylogenetic Analyses ◽  
Regulatory Region ◽  
Selection Marker ◽  
Pcr Analysis ◽  
Transformation System ◽  
Homologous Transformation ◽  
Reductase Gene ◽  
Penicillium Griseoroseum

Penicillium griseoroseum has been studied because of its efficient pectinases production. In this work, the Penicillium griseoroseum nitrate reductase gene was characterized, transcriptionaly analyzed in different nitrogen sources, and used to create a phylogenetic tree and to develop a homologous transformation system. The regulatory region contained consensus signals involved in nitrogen metabolism and the structural region was possibly interrupted by 6 introns coding for a deduced protein with 864 amino acids. RT–PCR analysis revealed high amounts of niaD transcript in the presence of nitrate. Transcription was repressed by ammonium, urea, and glutamine showing an efficient turnover of the niaD mRNA. Phylogenetics analysis showed distinct groups clearly separated in accordance with the classical taxonomy. A mutant with a 122-bp deletion was used in homologous transformation experiments and showed a transformation frequency of 14 transformants/µg DNA. All analyzed transformants showed that both single- and double-crossover recombination occurred at the niaD locus. The establishment of this homologous transformation system is an essential step for the improvement of pectinase production in Penicillium griseoroseum.Key words: nitrate reductase, nitrogen metabolism, Penicillium griseoroseum, phylogenetic analysis, homologous transformation.

Transformation of Penicillium griseoroseum nitrate reductase mutant with the nia gene from Fusarium oxysporum

1998 ◽  
Vol 44 (5) ◽  
pp. 487-489 ◽  
Author(s):  
Marisa V de Queiroz ◽  
Andréa O Barros ◽  
Everaldo G de Barros ◽  
Walter V Guimarães ◽  
Elza F de Araújo
Keyword(s):  
Nitrate Reductase ◽  
Fusarium Oxysporum ◽  
Structural Gene ◽  
Dna Hybridization ◽  
Transformation System ◽  
Genetic Studies ◽  
Penicillium Griseoroseum ◽  
Heterologous Transformation ◽  
Tandem Arrays

A heterologous transformation system for Penicillium griseoroseum has been developed. This system is based on nia, the structural gene from Fusarium oxysporum encoding nitrate reductase. Penicillium griseoroseum niaD mutants have been selected from chlorate-resistant colonies. Among 24 chlorate-resistant colonies analyzed, 2 were confirmed to be niaD mutants. Transformation frequencies of 8 transformants/µg of DNA were obtained. DNA hybridization analyses of five transformants showed distinct integration patterns of the plasmid and in all of them the integration occurred at tandem arrays. The transformation system established in this work will be useful for genetic studies of the pectinolytic complex genes from P. griseoroseum.Key words: Penicillium griseoroseum, heterologous transformation, nitrate reductase.

scholarly journals Development of a transformation system for Penicillium brevicompactum based on the Fusarium oxysporum nitrate reductase gene

2005 ◽  
Vol 36 (2) ◽  
Author(s):  
Maurílio Antônio Varavallo ◽  
Marisa Vieira de Queiroz ◽  
Jorge Fernando Pereira ◽  
Ronney Adriano Ribeiro ◽  
Marcos Antônio Soares ◽  
...  
Keyword(s):  
Nitrate Reductase ◽  
Fusarium Oxysporum ◽  
Transformation System ◽  
Nitrate Reductase Gene ◽  
Reductase Gene ◽  
Penicillium Brevicompactum

scholarly journals Molecular Epidemiology and Whole-Genome Analysis of Bovine Foamy Virus in Japan

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1017
Author(s):  
Hirohisa Mekata ◽  
Tomohiro Okagawa ◽  
Satoru Konnai ◽  
Takayuki Miyazawa
Keyword(s):  
Phylogenetic Analyses ◽  
Foamy Virus ◽  
Pcr Analysis ◽  
Whole Genome ◽  
Genome Sequences ◽  
Whole Genome Analysis ◽  
Virus Family ◽  
Bovine Foamy Virus ◽  
Novel Genotype

Bovine foamy virus (BFV) is a member of the foamy virus family in cattle. Information on the epidemiology, transmission routes, and whole-genome sequences of BFV is still limited. To understand the characteristics of BFV, this study included a molecular survey in Japan and the determination of the whole-genome sequences of 30 BFV isolates. A total of 30 (3.4%, 30/884) cattle were infected with BFV according to PCR analysis. Cattle less than 48 months old were scarcely infected with this virus, and older animals had a significantly higher rate of infection. To reveal the possibility of vertical transmission, we additionally surveyed 77 pairs of dams and 3-month-old calves in a farm already confirmed to have BFV. We confirmed that one of the calves born from a dam with BFV was infected. Phylogenetic analyses revealed that a novel genotype was spread in Japan. In conclusion, the prevalence of BFV in Japan is relatively low and three genotypes, including a novel genotype, are spread in Japan.

scholarly journals Biological and Molecular Characteristics of a Novel Partitivirus Infecting the Edible Fungus Lentinula edodes

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 726-733 ◽  
Author(s):  
Mengpei Guo ◽  
Yinbing Bian ◽  
Jinjie Wang ◽  
Gangzheng Wang ◽  
Xiaolong Ma ◽  
...  
Keyword(s):  
Lentinula Edodes ◽  
Control Strategies ◽  
Phylogenetic Analyses ◽  
Pcr Analysis ◽  
Molecular Characteristics ◽  
Rt Pcr ◽  
Edible Fungus ◽  
Qpcr Analysis ◽  
Disease Diagnostics ◽  
Wild Strains

A new partitivirus named Lentinula edodes partitivirus 1 (LePV1) was isolated from a diseased L. edodes strain with severe degeneration of the mycelium and imperfect browning in bag cultures. The nucleotide sequences of LePV1 dsRNA-1 and dsRNA-2 were determined; they were 2,382 bp and 2,245 bp in length, and each contained a single ORF encoding RNA-dependent RNA polymerase (RdRp) and coat protein (CP), respectively. The purified virus preparation contained isometric particles 34 nm in diameter encapsidating these dsRNAs. Phylogenetic analyses showed LePV1 to be a new member of Betapartitivirus, with the RdRp sequence most closely related to Grapevine partitivirus. RT-PCR analysis showed that 27 of the 56 Chinese L. edodes core collection strains carry LePV1, with the virus being more common in wild strains than cultivated strains. In addition, qPCR analysis suggested that coinfection with L. edodes mycovirus HKB (LeV-HKB) could increase replication of the RdRp gene of LePV1. This study may be essential for the development of more accurate disease diagnostics and the formulation of control strategies for viral diseases in L. edodes.

Development of a Homologous Transformation System forAspergillus aculeatusBased on thesCGene Encoding ATP-Sulfurylase

2009 ◽  
Vol 73 (5) ◽  
pp. 1197-1199 ◽  
Author(s):  
Hiromi ADACHI ◽  
Shuji TANI ◽  
Shin KANAMASA ◽  
Jun-ichi SUMITANI ◽  
Takashi KAWAGUCHI
Keyword(s):  
Transformation System ◽  
Atp Sulfurylase ◽  
Homologous Transformation
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