Phenotypic identification ofAeromonasgenomospecies from clinical and environmental sources

1998 ◽  
Vol 44 (2) ◽  
pp. 103-108 ◽  
Author(s):  
N Borrell ◽  
M J Figueras ◽  
J Guarro
Keyword(s):  
Drinking Water ◽  
Aeromonas Hydrophila ◽  
Sea Water ◽  
Aeromonas Veronii ◽  
Aeromonas Caviae ◽  
Food Borne ◽  
Lakes And Reservoirs ◽  
Treated Drinking Water ◽  
Prevalent Species ◽  
Isolated Species

A collection of 983 Aeromonas isolates from environmental and clinical sources have been identified to the genomospecies level. A phenotypic method identified 93% of the strains. The use of citrate and the production of acid from sorbitol enabled the members of the Aeromonas hydrophila complex to be separated. The most common genomospecies from intestinal sources were Aeromonas veronii biotype sobria and Aeromonas caviae. The former, together with A. hydrophila,was the most frequently isolated species of extraintestinal origin. Most pathogenic species were very prevalent in environmental samples, with A. veronii biotype sobria being the most common in lakes and reservoirs (41.5%) and in treated drinking water (25.0%), and A. caviae was the most common in sea water (26.0%) and milk products (35.5%). Aeromonas hydrophila (18.1%) was the second most prevalent species isolated in untreated drinking water. Since Aeromonas infections are generally regarded as a water- and food-borne diseases, the high environmental prevalence of these pathogenic genomospecies should be regarded as an important threat to public health.Key words: Aeromonas, food, water, clinical, Spain.

Importance of flagella and enterotoxins for Aeromonas virulence in a mouse model

2007 ◽  
Vol 53 (2) ◽  
pp. 261-269 ◽  
Author(s):  
Keya Sen ◽  
Dennis Lye
Keyword(s):  
Drinking Water ◽  
Virulence Factor ◽  
Genetic Characterization ◽  
Aeromonas Veronii ◽  
Aeromonas Caviae ◽  
Polymerase Chain ◽  
Immunocompromised Mice ◽  
Immunocompromised Hosts ◽  
Virulence Factor Genes

A genetic characterization of eight virulence factor genes, elastase, lipase, polar flagella (flaA/flaB, flaG), lateral flagella (lafA), and the enterotoxins alt, act, and ast, was performed using polymerase chain reaction with 55 drinking water and nine clinical isolates. When 16 Aeromonas hydrophila strains, seven Aeromonas veronii strains, and seven Aeromonas caviae strains exhibiting different combinations of virulence factor genes were tested in immunocompromised mice by intraperitoneal injection, only those strains that had one or more of the enterotoxins flaA, flaB, and either flaG or lafA showed signs of being virulent. The correlation was seen in 97% (29/30) of the strains, which included strains from drinking water. Thus, Aeromonas water isolates have the potential to be pathogenic in immunocompromised hosts.

scholarly journals Caracterização de Aeromonas spp isoladas de neonatos hospitalizados

2008 ◽  
Vol 41 (2) ◽  
pp. 179-182 ◽  
Author(s):  
Christiane Soares Pereira ◽  
Simone Duarte Amorim ◽  
André Felipe das Mercês Santos ◽  
Cristhiane Moura Falavina dos Reis ◽  
Grace Nazareth Diogo Theophilo ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Rio De Janeiro ◽  
Aeromonas Veronii ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae

Aeromonas spp é reconhecida como patogênica para o homem após o consumo de água e alimentos contaminados. Na presente investigação, foram avaliadas 2.323 amostras de swabs retais de neonatos hospitalizados no Rio de Janeiro objetivando o isolamento de Aeromonas. As amostras foram coletadas e enviadas ao Laboratório de Referência Nacional de Cólera e outras enteroinfecções bacterianas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz. Os swabs foram submetidos ao enriquecimento em água peptonada alcalina adicionada de 1% de cloreto de sódio (NaCl) e água peptonada alcalina adicionada de 3% de NaCl (37ºC/18-24h) e semeadas em agar seletivo para Pseudomonas aeromonas (Agar GSP). Foram isoladas 56 cepas de Aeromonas assim distribuídas: Aeromonas caviae (42,8%), Aeromonas media (25%), Aeromonas veronii biogrupo sobria (10,7%), Aeromonas hydrophila (9%), Aeromonas veronii biogrupo veronii (5,3%), Aeromonas sobria (1,8%), Aeromonas jandaei (1,8%), Aeromonas schubertii (1,8%) e Aeromonas sp (1,8%). Foi observada resistência a uma ou mais drogas antimicrobianas em 26,8% das cepas. Considerando a relevância de Aeromonas torna-se urgente alertar sobre sua importância para o controle de infecções hospitalares.

scholarly journals Ocorrência de Aeromonas spp. em alimentos de origem animal e sua importância em saúde pública

2015 ◽  
Vol 82 (0) ◽  
Author(s):  
Alana Borges Tavares ◽  
Natacha Deboni Cereser ◽  
Cláudio Dias Timm
Keyword(s):  
Aeromonas Hydrophila ◽  
Aeromonas Veronii ◽  
Aeromonas Caviae

Aeromonas spp. são bactérias Gram negativas, opor-tunistas, de natureza ubíqua, isoladas principalmente de amostras de água. Até o presente momento foram reconhecidas 31 espé-cies, sendo as de maior importância médica Aeromonas hydrophila, Aeromonas caviae e Aeromonas veronii. A patogenicidade do gênero é considerada multifatorial, sendo este produtor de diversos tipos de toxinas e com envolvimento de outros fatores capazes de facilitar a penetração e o estabelecimento do agente no hospedeiro, causando doença. O objetivo desta revisão é elucidar o papel dos alimentos de origem animal como fontes de contaminação de bactérias do gênero Aeromonas para o ser humano. Isolamentos de aeromonas de diversos produtos de origem animal têm sido relatados, como carne, leite e seus derivados, além de frutos do mar, e em ambientes de processamento, como abatedouros, frigorífcos e laticínios. Tem-se buscado determinar fontes de contaminação dos alimentos, e a água foi definida como o principal disseminador. Aeromonas já foi defnida como sendo a causadora de diversas enfermidades, desde afecções gastrointestinais até casos de meningite e morte. Considerando os alimentos de origem animal como importantes veículos de transmissão para o ser humano e o reconhecimento da água como fonte de disseminação do agente, torna-se imprescindível o tratamento adequado da água utilizada nos estabelecimentos processadores de alimentos para a segurança alimentar.

scholarly journals BACTÉRIAS DO GÊNERO AEROMONAS NO FLUXOGRAMA DE BENEFICIAMENTO DO LEITE TIPO A E SEU COMPORTAMENTO FRENTE À AÇÃO DE ANTIMICROBIANOS

2006 ◽  
Vol 73 (3) ◽  
pp. 271-276
Author(s):  
M.S. Carneiro ◽  
O.D Rossi Junior
Keyword(s):  
Aeromonas Hydrophila ◽  
Aeromonas Veronii ◽  
Aeromonas Sobria ◽  
Aeromonas Caviae

RESUMO Objetivando verificar os possíveis pontos de contaminação e disseminação de bactérias do gênero Aeromonas, foram analisadas amostras de leite colhidas em diferentes pontos do fluxograma de beneficiamento em uma granja de leite tipo A. De 80 amostras analisadas, Aeromonas spp. foram isoladas em 37 (46,25%) delas. Foram colhidas 20 amostras de cada ponto da linha de produção e revelaram-se positivas no isolamento, 90% (18/20) das amostras de leite cru, 30% (6/ 20) das de leite da saída do pasteurizador, 40% (8/20) das de leite do tanque de abastecimento da máquina de empacotar e 25% (5/20) das amostras do leite pronto para consumo. Dentre as espécies foram identificadas Aeromonas sobria em 40,54% das amostras, Aeromonas hydrophila e Aeromonas caviae em 21,62% delas, Aeromonas veronii em 10,81% e Aeromonas schubertii em 2,7%. Das cepas isoladas, 37 foram submetidas a testes de sensibilidade a antimicrobianos. Destas, 100% foram resistentes à ampicilina, 75,67% à cefalotina, 64,86% à cefoxitina e 48,64% ao aztreonam. As drogas as quais as cepas mostraram-se menos resistentes foram, cloranfenicol, netilmicina, amicacina, gentamicina, tetraciclina e tobramicina. Os resultados obtidos demonstram que bactérias do gênero Aeromonas resistentes a vários antimicrobianos podem estar presentes no leite tipo A, o que faz com que este produto possa determinar risco à saúde da população consumidora, e que deve servir de alerta aos serviços de saúde pública.

Detection and Health Risk Associated with Low Virus Concentration in Drinking Water

1985 ◽  
Vol 17 (10) ◽  
pp. 97-103 ◽  
Author(s):  
P. Payment ◽  
M. Trudel
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Health Problem ◽  
Conventional Treatment ◽  
Probabilistic Approach ◽  
Detection Methods ◽  
Virus Concentration ◽  
The Subject ◽  
Treated Drinking Water ◽  
Dangerous Level

During the last decade, with the amelioration of the detection methods and the increasing number of studies on the subject, the isolation of viruses in treated drinking water has been reported more frequently than ever. These reports have in common the very low number of viruses isolated and these viruses are usually found only after concentration procedures involving several hundred liters of water. Our own studies have shown that during the conventional treatment of drinking water 99.998% of the indigenous viruses are removed. The residual viral fraction does not exceed 10 viruses per 1 000 liters of water. Using a probabilistic approach this viral concentration in drinking water is well below any dangerous level of enteric viruses in water and the presence of these viruses should not be considered as a health problem but more as the limit of the water treatment methodology.

Hygienic Aspects of the Use of Desalinated Sea Water for Drinking and Household Purposes: A Literature Review

2021 ◽  
pp. 26-32
Author(s):  
OO Sinitsyna ◽  
VV Turbinsky ◽  
TM Ryashentseva ◽  
EP Lavrik
Keyword(s):  
Drinking Water ◽  
Fresh Water ◽  
Land Surface ◽  
Sea Water ◽  
Additional Treatment ◽  
Toxic Substances ◽  
Seawater Desalination ◽  
Preliminary Preparation ◽  
And Migration ◽  
Desalination Plants

Background. Uneven distribution of fresh water sources on the land surface encourages a search for effective techniques of potable water preparation by desalination of seawater. Hygienic issues of such desalination methods as distillation, reverse osmosis, electrodialysis, and ion exchange have been investigated by now and appropriate limitations, requirements, and additional measures to ensure safety of desalinated drinking water have been established. Objective. To summarize and systematize the results of studying characteristics of various methods of seawater desalination for its further use for drinking and household purposes. Materials and methods. We conducted a systematic review of studies published in Russian and in English, found in the PubMed and Web of Science databases, and selected 40 literary sources containing an empirical assessment of effectiveness of seawater desalination and preparation of drinking water. We also scrutinized regulatory documents and guidelines of domestic sanitary legislation. The research results were systematized by the main desalination methods. Results and discussion. We established that the use of seawater for the preparation of fresh water for drinking and household purposes is becoming increasingly widespread around the world. Drinking water obtained from seawater, in all cases, requires additional treatment and measures to optimize its mineral composition and protect against microorganisms. Conclusion. The main challenges of ensuring sanitary and epidemiological wellbeing of the population when using desalinated seawater for drinking and household purposes include selection of a source, arrangement of sites of water intake properly protected from natural and man-made pollution, substantiation of techniques and modes of preliminary preparation of source seawater adequate to its composition, basic desalination, ensuring safety of products of destruction and migration of toxic substances from reagents and materials of desalination plants, additional conditioning with the necessary elements and disinfection of the prepared water, as well as environmental protection from desalination waste.

scholarly journals Envolvimento de Aeromonas em surto de doença diarréica aguda em São Bento do Una, Pernambuco

2006 ◽  
Vol 39 (2) ◽  
pp. 217-220 ◽  
Author(s):  
Ernesto Hofer ◽  
Cristhiane Moura Falavina dos Reis ◽  
Grace Nazareth Diogo Theophilo ◽  
Valdelúcia Oliveira Cavalcanti ◽  
Nancy Veloso de Lima ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Salmonella Spp ◽  
Aeromonas Veronii ◽  
Aeromonas Caviae ◽  
Shigella Spp

No primeiro semestre de 2004, ocorreu um surto de diarréia em São Bento do Una, Pernambuco, registrando-se 2.170 casos. Nas 582 coproculturas realizadas, 145 (25%) revelaram um enteropatógeno bacteriano, destacando 114 casos (19,5%) com a participação de Aeromonas, representadas por Aeromonas caviae (57/9,8%), Aeromonas veronii biovar sobria (23/3,9%), Aeromonas veronii biovar veronii (15/2,6%) e outras espécies (19/3,2%). Nos 31 episódios restantes (5,3%), foram detectados: V. cholerae O1 Ogawa toxigênico (18/3,1%), Salmonella spp (8/1,4%), Shigella spp (3/0,5%) e Vibrio cholerae não O1/não O139 (2/0,3%).

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