High production of ligninolytic enzymes from white rot fungi in cereal bran liquid medium

White rot fungi from the University of Alberta Mold Herbarium, identified as able to degrade aromatics from a study of PCB metabolism, were examined for production of ligninolytic enzymes. Production of lignin peroxidase, manganese peroxidase, laccase, and veratryl alcohol oxidase were monitored during growth in different media. Good growth but low enzyme production occurred in a glucose - malt extract - yeast extract medium. Media containing 2% cereal bran in 60 mM phosphate buffer supported high levels of laccase production, up to 13 000 U/L in Coriolopsis gallica UAMH 8260 and manganese peroxidase activity up to 1100 U/L in Bjerkandera adusta UAMH 8258. Cereal bran media supported higher laccase production than 2,5-xylidine and higher manganese peroxidase production than a medium containing manganous ion plus veratryl alcohol.Key words: cereal bran, laccase, manganese peroxidase, white rot fungi.

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Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea

Biotechnology Research International ◽

10.1155/2014/815495 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 16

Author(s):

K. Y. Usha ◽

K. Praveen ◽

B. Rajasekhar Reddy

Keyword(s):

Solid State ◽

Copper Sulphate ◽

White Rot Fungi ◽

Ligninolytic Enzymes ◽

Tween 80 ◽

Veratryl Alcohol ◽

White Rot ◽

Tween 20 ◽

Laccase Production ◽

Enhanced Production

The white rot fungi Stereum ostrea displayed a wide diversity in their response to supplemented inducers, surfactants, and copper sulphate in solid state fermentation. Among the inducers tested, 0.02% veratryl alcohol increased the ligninolytic enzyme production to a significant extent. The addition of copper sulphate at 300 μM concentration has a positive effect on laccase production increasing its activity by 2 times compared to control. Among the surfactants, Tween 20, Tween 80, and Triton X 100, tested in the studies, Tween 80 stimulated the production of ligninolytic enzymes. Biosorption of dyes was carried out by using two lignocellulosic wastes, rice bran and wheat bran, in 50 ppm of remazol brilliant blue and remazol brilliant violet 5R dyes. These dye adsorbed lignocelluloses were then utilized for the production of ligninolytic enzymes in solid state mode. The two dye adsorbed lignocelluloses enhanced the production of laccase and manganese peroxidase but not lignin peroxidase.

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The Influence of Inducers on the Coltricia cinnamomea Laccase Activity and its Ability to Degrade POME

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v13i2.29660 ◽

2021 ◽

Vol 13 (2) ◽

pp. 243-249

Author(s):

Yohanes Bernard Subowo ◽

Arwan Sugiharto

Keyword(s):

Palm Oil ◽

Lignin Peroxidase ◽

Manganese Peroxidase ◽

Laccase Activity ◽

Ligninolytic Enzymes ◽

Veratryl Alcohol ◽

White Rot ◽

Growth Media ◽

Palm Oil Mill ◽

Low Activity

Some species of Basidiomycetes, specifically white rot groups, produce three ligninolytic enzymes, namely, Lignin Peroxidase (LiP), Manganese Peroxidase (MnP) and Laccase (Lac), which have low activity in degrading Palm Oil Mill Effluent (POME). The research objective was to obtain the data on the ability of the Coltricia cinnamomea to produce LiP, MnP, and Lac enzymes to degrade POME. This research also studied the effect of sucrose, alcohol, veratryl alcohol, CuSO4 and ZnSO4,as inducers. Isolates of Coltricia cinnamomea, which were stored in a PDA media at -20℃ were obtained from the Microbiology section of the Research Center for Biology (LIPI). Furthermore, the growth media used were DM, Bean sprout Extract (TE) and PDB. The result indicated that PDB is the most suitable growth media for the production of ligninolytic enzymes, because in this medium these enzymes showed the highest activity. It was also observed that sucrose increased the laccase activity by 40.80%. Furthermore, Coltricia cinnamomea was able to reduce the concentration of Poly R-478 by 60.74%, after the addition of ZnSO4. In addition, it degraded and decreased the color and COD of POME, by 72.63% and 91.19% respectively, after the addition of veratryl alcohol, and incubation for 10 days. Therefore, this fungus can be used to degrade POME in order to prevent environmental pollution. Coltricia cinnamomea has not been used for POME degradation. By using Coltricia cinnamomea, we obtained new data regarding the activity of laccase and its ability to degrade POME.

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Lignin-degrading activity and ligninolytic enzymes of different white-rot fungi: effects of manganese and malonate

Canadian Journal of Botany ◽

10.1139/b97-007 ◽

1997 ◽

Vol 75 (1) ◽

pp. 61-71 ◽

Cited By ~ 31

Author(s):

Tamara Vares ◽

Annele Hatakka

Keyword(s):

Lignin Peroxidase ◽

Manganese Peroxidase ◽

White Rot Fungi ◽

Ligninolytic Enzymes ◽

Growth Conditions ◽

Fungal Species ◽

White Rot ◽

Trametes Hirsuta ◽

Air Atmosphere ◽

Trametes Trogii

Ten species of white-rot fungi, mainly belonging to the family Polyporaceae (Basidiomycotina), were studied in terms of their ability to degrade14C-ring labelled synthetic lignin and secrete ligninolytic enzymes in liquid cultures under varying growth conditions. Lignin mineralization by the fungi in an air atmosphere did not exceed 14% within 29 days. Different responses to the elevated Mn2+concentration and the addition of a manganese chelator (sodium malonate) were observed among various fungal species. This could be related with the utilization of either lignin peroxidase (LiP) or manganese peroxidase (MnP) for lignin depolymerization, i.e., some fungi apparently had an LiP-dominating ligninolytic system and others an MnP-dominating ligninolytic system. The LiP isoforms were purified from Trametes gibbosa and Trametes trogii. Isoelectric focusing of purified ligninolytic enzymes revealed the expression of numerous MnP isoforms in Trametes gibbosa, Trametes hirsuta, Trametes trogii, and Abortiporus biennis grown under a high (50-fold) Mn2+level (120 μM) with the addition of the chelator. In addition, two to three laccase isoforms were detected. Key words: white-rot fungi, lignin degradation, lignin peroxidase, manganese peroxidase, manganese, malonate.

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Biological pretreatment of lignocelluloses with white-rot fungi and its applications: A review

BioResources ◽

10.15376/biores.6.4.isroi ◽

2011 ◽

Vol 6 (4) ◽

pp. 5224-5259

Author(s):

Isroi ◽

Ria Millati ◽

Siti Syamsiah ◽

Claes Niklasson ◽

Muhammad Nur Cahyanto ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Manganese Peroxidase ◽

Animal Feed ◽

White Rot Fungi ◽

Ligninolytic Enzymes ◽

White Rot ◽

Chemical Pretreatment ◽

Biological Pretreatment ◽

Factors Affecting ◽

Ruminant Feed

Lignocellulosic carbohydrates, i.e. cellulose and hemicellulose, have abundant potential as feedstock for production of biofuels and chemicals. However, these carbohydrates are generally infiltrated by lignin. Breakdown of the lignin barrier will alter lignocelluloses structures and make the carbohydrates accessible for more efficient bioconversion. White-rot fungi produce ligninolytic enzymes (lignin peroxidase, manganese peroxidase, and laccase) and efficiently mineralise lignin into CO2 and H2O. Biological pretreatment of lignocelluloses using white-rot fungi has been used for decades for ruminant feed, enzymatic hydrolysis, and biopulping. Application of white-rot fungi capabilities can offer environmentally friendly processes for utilising lignocelluloses over physical or chemical pretreatment. This paper reviews white-rot fungi, ligninolytic enzymes, the effect of biological pretreatment on biomass characteristics, and factors affecting biological pretreatment. Application of biological pretreatment for enzymatic hydrolysis, biofuels (bioethanol, biogas and pyrolysis), biopulping, biobleaching, animal feed, and enzymes production are also discussed.

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Bioremediation of Direct Blue 14 and Extracellular Ligninolytic Enzyme Production by White Rot Fungi:PleurotusSpp.

BioMed Research International ◽

10.1155/2013/180156 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4 ◽

Cited By ~ 10

Author(s):

M. P. Singh ◽

S. K. Vishwakarma ◽

A. K. Srivastava

Keyword(s):

Enzymatic Activity ◽

Manganese Peroxidase ◽

Enzyme Production ◽

White Rot Fungi ◽

Ligninolytic Enzymes ◽

Dye Decolorization ◽

Ligninolytic Enzyme ◽

Enzymatic Activities ◽

White Rot ◽

Direct Blue

In the present investigation, four species of white rot fungi (Pleurotus), that is,P. flabellatus, P. florida, P. ostreatusandP. sajor-cajuwere used for decolorization of direct blue 14 (DB14). Among all four species ofPleurotus,P. flabellatusshowed the fastest decolorization in petri plates on different concentration, that is, 200 mg/L, 400 mg/L, and 600 mg/L. All these four species were also evaluated for extracellular ligninolytic enzymes (laccase and manganese peroxidase) production and it was observed that the twelve days old culture ofP. flabellatusshowed the maximum enzymatic activity, that is, 915.7 U/mL and 769.2 U/mL of laccase and manganese peroxidase, respectively. Other threePleurotusspecies took more time for dye decolorization and exhibited less enzymatic activities. The rate of decolorization of DB14 dye solution (20 mg/L) by crude enzymes isolated fromP. flabellatuswas very fast, and it was observed that up to 90.39% dye solution was decolorized in 6 hrs of incubation.

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Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts

Fermentation ◽

10.3390/fermentation7010019 ◽

2021 ◽

Vol 7 (1) ◽

pp. 19

Author(s):

Anastasia Zerva ◽

Nikolaos Tsafantakis ◽

Evangelos Topakas

Keyword(s):

Biomass Conversion ◽

White Rot Fungi ◽

Ligninolytic Enzymes ◽

Inhibitory Effect ◽

Biologically Active ◽

White Rot ◽

Oxidative Enzymes ◽

Industrial Residues ◽

Pure Compounds ◽

Wild Strains

White-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cellulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important application in cosmetic formulations, either as pure compounds or as standardized extracts. In the present work, three wild strains of Basidiomycetes fungi (Pleurotus citrinopileatus, Abortiporus biennis and Ganoderma resinaceum) from Greek habitats were grown in agro-industrial residues (oil mill wastewater, and corn cob) and evaluated for their anti-tyrosinase and antioxidant activity and for the production of biotechnologically relevant enzymes. P. citrinopileatus showed the most interesting tyrosinase inhibitory activity, while A. biennis showed the highest DPPH(2,2-diphenyl-1-picryl-hydrazyl) scavenging potential. Corn cobs were the most appropriate carbon source for maximizing the inhibitory effect of fungal biomasses on both activities, while the use of oil mill wastewater selectively increased the anti-tyrosinase potential of P. citrinopileatus culture filtrate. All strains were found to be preferential lignin degraders, similarly to most white-rot fungi. Bioinformatic analyses were performed on the proteome of the strains P. citrinopileatus and A. biennis, focusing on CAZymes with biotechnological relevance, and the results were compared with the enzyme activities of culture supernatants. Overall, all three strains showed strong production of oxidative enzymes for biomass conversion applications.

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