scholarly journals Bioremediation of Direct Blue 14 and Extracellular Ligninolytic Enzyme Production by White Rot Fungi:PleurotusSpp.

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
M. P. Singh ◽  
S. K. Vishwakarma ◽  
A. K. Srivastava
Keyword(s):  
Enzymatic Activity ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Dye Decolorization ◽  
Ligninolytic Enzyme ◽  
Enzymatic Activities ◽  
White Rot ◽  
Direct Blue

In the present investigation, four species of white rot fungi (Pleurotus), that is,P. flabellatus, P. florida, P. ostreatusandP. sajor-cajuwere used for decolorization of direct blue 14 (DB14). Among all four species ofPleurotus,P. flabellatusshowed the fastest decolorization in petri plates on different concentration, that is, 200 mg/L, 400 mg/L, and 600 mg/L. All these four species were also evaluated for extracellular ligninolytic enzymes (laccase and manganese peroxidase) production and it was observed that the twelve days old culture ofP. flabellatusshowed the maximum enzymatic activity, that is, 915.7 U/mL and 769.2 U/mL of laccase and manganese peroxidase, respectively. Other threePleurotusspecies took more time for dye decolorization and exhibited less enzymatic activities. The rate of decolorization of DB14 dye solution (20 mg/L) by crude enzymes isolated fromP. flabellatuswas very fast, and it was observed that up to 90.39% dye solution was decolorized in 6 hrs of incubation.

scholarly journals Ligninolytic Enzyme Production and Decolorization Capacity of Synthetic Dyes by Saprotrophic White Rot, Brown Rot, and Litter Decomposing Basidiomycetes

2020 ◽  
Vol 6 (4) ◽  
pp. 301
Author(s):  
Ivana Eichlerová ◽  
Petr Baldrian
Keyword(s):  
Enzyme Production ◽  
Ligninolytic Enzymes ◽  
Brown Rot ◽  
Dye Decolorization ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Mn Peroxidase ◽  
Taxonomic Groups

An extensive screening of saprotrophic Basidiomycetes causing white rot (WR), brown rot (BR), or litter decomposition (LD) for the production of laccase and Mn-peroxidase (MnP) and decolorization of the synthetic dyes Orange G and Remazol Brilliant Blue R (RBBR) was performed. The study considered in total 150 strains belonging to 77 species. The aim of this work was to compare the decolorization and ligninolytic capacity among different ecophysiological and taxonomic groups of Basidiomycetes. WR strains decolorized both dyes most efficiently; high decolorization capacity was also found in some LD fungi. The enzyme production was recorded in all three ecophysiology groups, but to a different extent. All WR and LD fungi produced laccase, and the majority of them also produced MnP. The strains belonging to BR lacked decolorization capabilities. None of them produced MnP and the production of laccase was either very low or absent. The most efficient decolorization of both dyes and the highest laccase production was found among the members of the orders Polyporales and Agaricales. The strains with high MnP activity occurred across almost all fungal orders (Polyporales, Agaricales, Hymenochaetales, and Russulales). Synthetic dye decolorization by fungal strains was clearly related to their production of ligninolytic enzymes and both properties were determined by the interaction of their ecophysiology and taxonomy, with a more relevant role of ecophysiology. Our screening revealed 12 strains with high decolorization capacity (9 WR and 3 LD), which could be promising for further biotechnological utilization.

scholarly journals Lignin-degrading activity and ligninolytic enzymes of different white-rot fungi: effects of manganese and malonate

1997 ◽  
Vol 75 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Tamara Vares ◽  
Annele Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Growth Conditions ◽  
Fungal Species ◽  
White Rot ◽  
Trametes Hirsuta ◽  
Air Atmosphere ◽  
Trametes Trogii

Ten species of white-rot fungi, mainly belonging to the family Polyporaceae (Basidiomycotina), were studied in terms of their ability to degrade14C-ring labelled synthetic lignin and secrete ligninolytic enzymes in liquid cultures under varying growth conditions. Lignin mineralization by the fungi in an air atmosphere did not exceed 14% within 29 days. Different responses to the elevated Mn2+concentration and the addition of a manganese chelator (sodium malonate) were observed among various fungal species. This could be related with the utilization of either lignin peroxidase (LiP) or manganese peroxidase (MnP) for lignin depolymerization, i.e., some fungi apparently had an LiP-dominating ligninolytic system and others an MnP-dominating ligninolytic system. The LiP isoforms were purified from Trametes gibbosa and Trametes trogii. Isoelectric focusing of purified ligninolytic enzymes revealed the expression of numerous MnP isoforms in Trametes gibbosa, Trametes hirsuta, Trametes trogii, and Abortiporus biennis grown under a high (50-fold) Mn2+level (120 μM) with the addition of the chelator. In addition, two to three laccase isoforms were detected. Key words: white-rot fungi, lignin degradation, lignin peroxidase, manganese peroxidase, manganese, malonate.

scholarly journals White-rot fungus: an updated review

2021 ◽  
pp. 202-217
Author(s):  
Jaspreet Kaur ◽  
Amar Pal Singh ◽  
Ajeet Pal Singh ◽  
Rajinderpal Kaur
Keyword(s):  
Stainless Steel ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
White Mold ◽  
White Rot Fungus ◽  
Impact Factors ◽  
Production Processes ◽  
Polyamide Fiber

The White Fungus, which causes white rot on tree trunks, belongs to the basidiomycetes. Research into the microbiology of White-rot fungi has focused on engineering processes related to factors such as cell growth and enzyme production processes, and to smaller, i.e., molecular biology. Many studies have been conducted to select issues with high or specific biodegradation performance in a variety of ways. Production inhibitors have been used to improve enzyme production. Investigators are investigating different carriers (Stainless Steel net, polyamide fiber net, fiberglass net and polyurethane foam) to impair P.chrysosporium ligninolytic enzyme production. In this review, Pathophysiology, Microbiology, impact factors, treatments and alternative uses show white mold formation in biotransformation. The white fungus is being investigated to produce biotechnology for the reduction of a broad spectrum, a natural pollutant based on lignin-deficient enzymes. This in particular covers the destruction of many wastes and environmental pollution, including wastewater, pesticides, toxic natural pollutants, chlorinated hydrocarbons, etc. It will be updated.

scholarly journals Biological pretreatment of lignocelluloses with white-rot fungi and its applications: A review

BioResources ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. 5224-5259
Author(s):  
Isroi ◽  
Ria Millati ◽  
Siti Syamsiah ◽  
Claes Niklasson ◽  
Muhammad Nur Cahyanto ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Manganese Peroxidase ◽  
Animal Feed ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot ◽  
Chemical Pretreatment ◽  
Biological Pretreatment ◽  
Factors Affecting ◽  
Ruminant Feed

Lignocellulosic carbohydrates, i.e. cellulose and hemicellulose, have abundant potential as feedstock for production of biofuels and chemicals. However, these carbohydrates are generally infiltrated by lignin. Breakdown of the lignin barrier will alter lignocelluloses structures and make the carbohydrates accessible for more efficient bioconversion. White-rot fungi produce ligninolytic enzymes (lignin peroxidase, manganese peroxidase, and laccase) and efficiently mineralise lignin into CO2 and H2O. Biological pretreatment of lignocelluloses using white-rot fungi has been used for decades for ruminant feed, enzymatic hydrolysis, and biopulping. Application of white-rot fungi capabilities can offer environmentally friendly processes for utilising lignocelluloses over physical or chemical pretreatment. This paper reviews white-rot fungi, ligninolytic enzymes, the effect of biological pretreatment on biomass characteristics, and factors affecting biological pretreatment. Application of biological pretreatment for enzymatic hydrolysis, biofuels (bioethanol, biogas and pyrolysis), biopulping, biobleaching, animal feed, and enzymes production are also discussed.

Effect of aromatic compounds on growth and ligninolytic enzyme production of two white rot fungi Ceriporiopsis subvermispora and Cyathus stercoreus

1998 ◽  
Vol 44 (9) ◽  
pp. 872-885 ◽  
Author(s):  
Anand Sethuraman ◽  
Danny E Akin ◽  
Jason G Eisele ◽  
Karl-Erik L Eriksson
Keyword(s):  
Cinnamic Acid ◽  
Aromatic Compounds ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
White Rot Fungi ◽  
Hyphal Growth ◽  
White Rot ◽  
Ceriporiopsis Subvermispora ◽  
Cyathus Stercoreus ◽  
Benzaldehyde Derivatives

Seven benzoic acid, ten cinnamic acid, and five benzaldehyde derivatives were tested for their effects on hyphal growth and production of laccase and manganese peroxidase by Ceriporiopsis subvermispora FP 90031-sp and Cyathus stercoreus ATCC 36910. Derivatives tested included phenolic compounds and their corresponding unsubstituted and O-methylated derivatives. Benzaldehyde derivatives were more toxic to both fungi than the corresponding benzoic and cinnamic acid derivatives. Hyphal growth was generally increased at a low concentration of 1 mM, while higher concentrations of 5-10 mM mostly resulted in less or no growth. Hyphal growth and enzyme production response were compound specific. However, generally monomethoxylated compounds were more toxic than compounds with an additional methoxyl group. Cyathus stercoreus was more sensitive than Ceriporiopsis subvermispora to most of the compounds tested and thus showed poorer growth. Cyathus stercoreus produced higher concentrations of manganese peroxidase than Ceriporiopsis subvermispora for all the compounds tested, whereas laccase activity was higher in Ceriporiopsis subvermispora for most of the compounds tested. Di- and tri-methoxylated compounds induced more laccase and manganese peroxidase activities than the corresponding hydroxylated derivatives. At 1 mM levels, 3,4-dimethoxycinnamic acid induced the greatest increase in laccase production for Ceriporiopsis subvermispora and Cyathus stercoreus (245 and 290% of control, respectively). Syringic acid induced manganese peroxidase (MnP) to 536% of that in control for Ceriporiopsis subvermispora, and both 3,4-dimethoxycinnamic acid and 3,4,5-trimethoxycinnamic acid induced MnP to over 300% of control for Cyathus stercoreus. The results provide a body of information on the effects of specific aromatic compounds on two potentially industrially important fungi. Key words: biomass conversion, aromatic compounds, white rot fungi, fungal growth, enzyme production.

Ligninolytic Activity of White Rot Fungi from GGV campus as Bioremediation of Organic Pollutants

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Sushil Kumar Shahi ◽  
Nikki Agrawal
Keyword(s):  
Organic Pollutants ◽  
Organic Pollutant ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Ligninolytic Enzyme ◽  
White Rot ◽  
Ligninolytic Activity ◽  
The North ◽  
Physiological Group ◽  
Wide Range

White rot fungi constitute a diverse physiological group are capable of transforming and mineralizing a wide range of organopollutants. The Ligninolytic enzymes of white rot fungi are substrate specific, essential for Lignin degradation and organic pollutant remediation. In the present study white rot fungi were collected from the north region of Chhattisgarh of Guru Ghasidas Vishwavidyalaya (GGV) Campus. 40 species were collected and isolated then Qualitative and Quantitative screening for the Ligninolytic enzyme assaywere carried out. Out of 40 species, 5 species show potent Ligninolytic activity. In future we can utilize these fungi for the degradation of organic pollutants.

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