scholarly journals Evaluation of Basidiomycetes Wild Strains Grown in Agro-Industrial Residues for Their Anti-Tyrosinase and Antioxidant Potential and for the Production of Biocatalysts

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 19
Author(s):  
Anastasia Zerva ◽  
Nikolaos Tsafantakis ◽  
Evangelos Topakas
Keyword(s):  
Biomass Conversion ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Inhibitory Effect ◽  
Biologically Active ◽  
White Rot ◽  
Oxidative Enzymes ◽  
Industrial Residues ◽  
Pure Compounds ◽  
Wild Strains

White-rot basidiomycetes are the only microorganisms with the ability to produce both hydrolytic (cellulases and hemicellulases) and oxidative (ligninolytic) enzymes for degrading cellulose/hemicellulose and lignin. In addition, they produce biologically active natural products with important application in cosmetic formulations, either as pure compounds or as standardized extracts. In the present work, three wild strains of Basidiomycetes fungi (Pleurotus citrinopileatus, Abortiporus biennis and Ganoderma resinaceum) from Greek habitats were grown in agro-industrial residues (oil mill wastewater, and corn cob) and evaluated for their anti-tyrosinase and antioxidant activity and for the production of biotechnologically relevant enzymes. P. citrinopileatus showed the most interesting tyrosinase inhibitory activity, while A. biennis showed the highest DPPH(2,2-diphenyl-1-picryl-hydrazyl) scavenging potential. Corn cobs were the most appropriate carbon source for maximizing the inhibitory effect of fungal biomasses on both activities, while the use of oil mill wastewater selectively increased the anti-tyrosinase potential of P. citrinopileatus culture filtrate. All strains were found to be preferential lignin degraders, similarly to most white-rot fungi. Bioinformatic analyses were performed on the proteome of the strains P. citrinopileatus and A. biennis, focusing on CAZymes with biotechnological relevance, and the results were compared with the enzyme activities of culture supernatants. Overall, all three strains showed strong production of oxidative enzymes for biomass conversion applications.

Distribution of ligninolytic enzymes in selected white-rot fungi

1996 ◽  
Vol 41 (3) ◽  
pp. 264-266 ◽  
Author(s):  
F. Nerud ◽  
Z. Mišurcová
Keyword(s):  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot

Mycoremediation of Lignocelluloses

Biotechnology ◽  
2019 ◽  
pp. 1086-1108
Author(s):  
Saritha Vara
Keyword(s):  
Spatial Distribution ◽  
Animal Feed ◽  
Space Exploration ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Mechanisms Of Action ◽  
White Rot ◽  
Lignocellulolytic Enzymes ◽  
Biotechnological Applications ◽  
Potential Use

The most abundant aromatic biopolymer on earth Lignin is extremely recalcitrant to degradation. It creates a barrier to solutions or enzymes by linking to both hemicellulose and cellulose preventing the penetration of lignocellulolytic enzymes into the interior lignocellulosic structure. Global attention has been gained by fungi owing to the potential use of their versatile enzymes for agriculture, medicines, industries and bioremediation. The combination of extracellular ligninolytic enzymes, mediators, organic acids and accessory enzymes make some of the basidiomycete white-rot fungi to be able to degrade lignin efficiently. This review describes remediation of lignocelluloses by fungi, properties of fungi, their spatial distribution and the mechanisms of action which render them attractive candidates in biotechnological applications like biopulping, animal feed, genetic engineering and space exploration.

scholarly journals Influence of white-rot fungi on chemical composition and in vitro digestibility of lignocellulosic agro-industrial residues

2014 ◽  
Vol 8 (28) ◽  
pp. 2724-2732 ◽  
Author(s):  
Braga Pereira Bento Cludia ◽  
Soares da Silva Juliana ◽  
Teixeira Rodrigues Marcelo ◽  
Catarina Megumi Kasuya Maria ◽  
Cuquetto Mantovani Hilrio
Keyword(s):  
Chemical Composition ◽  
White Rot Fungi ◽  
White Rot ◽  
In Vitro Digestibility ◽  
Industrial Residues

scholarly journals Induction of Extracellular Hydroxyl Radical Production by White-Rot Fungi through Quinone Redox Cycling

2009 ◽  
Vol 75 (12) ◽  
pp. 3944-3953 ◽  
Author(s):  
Víctor Gómez-Toribio ◽  
Ana B. García-Martín ◽  
María J. Martínez ◽  
Ángel T. Martínez ◽  
Francisco Guillén
Keyword(s):  
Hydroxyl Radical ◽  
Fenton Reaction ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot ◽  
Oh Radicals ◽  
Production Rates ◽  
Pycnoporus Cinnabarinus ◽  
Simple Strategy ◽  
The One

ABSTRACT A simple strategy for the induction of extracellular hydroxyl radical (OH) production by white-rot fungi is presented. It involves the incubation of mycelium with quinones and Fe3+-EDTA. Succinctly, it is based on the establishment of a quinone redox cycle catalyzed by cell-bound dehydrogenase activities and the ligninolytic enzymes (laccase and peroxidases). The semiquinone intermediate produced by the ligninolytic enzymes drives OH production by a Fenton reaction (H2O2 + Fe2+ → OH + OH− + Fe3+). H2O2 production, Fe3+ reduction, and OH generation were initially demonstrated with two Pleurotus eryngii mycelia (one producing laccase and versatile peroxidase and the other producing just laccase) and four quinones, 1,4-benzoquinone (BQ), 2-methoxy-1,4-benzoquinone (MBQ), 2,6-dimethoxy-1,4-benzoquinone (DBQ), and 2-methyl-1,4-naphthoquinone (menadione [MD]). In all cases, OH radicals were linearly produced, with the highest rate obtained with MD, followed by DBQ, MBQ, and BQ. These rates correlated with both H2O2 levels and Fe3+ reduction rates observed with the four quinones. Between the two P. eryngii mycelia used, the best results were obtained with the one producing only laccase, showing higher OH production rates with added purified enzyme. The strategy was then validated in Bjerkandera adusta, Phanerochaete chrysosporium, Phlebia radiata, Pycnoporus cinnabarinus, and Trametes versicolor, also showing good correlation between OH production rates and the kinds and levels of the ligninolytic enzymes expressed by these fungi. We propose this strategy as a useful tool to study the effects of OH radicals on lignin and organopollutant degradation, as well as to improve the bioremediation potential of white-rot fungi.

scholarly journals Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
K. Y. Usha ◽  
K. Praveen ◽  
B. Rajasekhar Reddy
Keyword(s):  
Solid State ◽  
Copper Sulphate ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Tween 80 ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Tween 20 ◽  
Laccase Production ◽  
Enhanced Production

The white rot fungi Stereum ostrea displayed a wide diversity in their response to supplemented inducers, surfactants, and copper sulphate in solid state fermentation. Among the inducers tested, 0.02% veratryl alcohol increased the ligninolytic enzyme production to a significant extent. The addition of copper sulphate at 300 μM concentration has a positive effect on laccase production increasing its activity by 2 times compared to control. Among the surfactants, Tween 20, Tween 80, and Triton X 100, tested in the studies, Tween 80 stimulated the production of ligninolytic enzymes. Biosorption of dyes was carried out by using two lignocellulosic wastes, rice bran and wheat bran, in 50 ppm of remazol brilliant blue and remazol brilliant violet 5R dyes. These dye adsorbed lignocelluloses were then utilized for the production of ligninolytic enzymes in solid state mode. The two dye adsorbed lignocelluloses enhanced the production of laccase and manganese peroxidase but not lignin peroxidase.

scholarly journals Biodegradability of Extractives in Sapwood and Heartwood from Scots Pine by Sapstain and White-Rot Fungi

Holzforschung ◽  
1999 ◽  
Vol 53 (3) ◽  
pp. 247-252 ◽  
Author(s):  
M.J. Martínez-Inigo ◽  
P. Immerzeel ◽  
A. Gutierrez ◽  
J.C. del Río ◽  
R. Sierra-Alvarez
Keyword(s):  
Scots Pine ◽  
White Rot Fungi ◽  
Inhibitory Effect ◽  
Resin Acids ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Degradation ◽  
Steryl Esters ◽  
Funalia Trogii ◽  
Total Extractives

SummaryThe fungal degradation of lipophilic extractives in sapwood and heartwood from Scots pine (Pinus sylvestris) was studied. In sapwood, the white rot fungi,Bjerkanderasp. andFunalia trogii, removed higher amounts of extractives than the sapstain strains,Ophiostoma ainoaeandCeratocystis allantospora. Triglycerides, long chain fatty acids, steryl esters and waxes in pine sapwood were almost completely degraded by all the fungi. Sterols and resin acids were also extensively degraded by the white rot strains; however, these components were not or only poorly removed by the sapstain fungi. The removal of total extractives by all the fungal strains was higher in sapwood as compared to heartwood. The highly concentrated extractive fraction in pine heartwood mainly consists of resin acids. As observed in sapwood, sapstain were also poorly effective in the degradation of the resin acids present in heartwood. The fungal degradation of heartwood extractives was not only limited by the degradative ability of the various test microorganisms, but also by the inhibitory effect exerted by the extractive fraction. The white rot fungusF. trogiiwas particularly inhibited on heartwood.Bjerkanderasp. showed a higher tolerance to toxic extractives and was the most efficient fungus in degrading extractive constituents in both Scots pine heartwood and sapwood. Therefore,Bjerkanderasp. strain BOS55 should be considered as a potential agent for pitch control in pulp and paper manufacture.

Oxidative dechlorination of methoxychlor by ligninolytic enzymes from white-rot fungi

Chemosphere ◽  
2004 ◽  
Vol 55 (4) ◽  
pp. 641-645 ◽  
Author(s):  
Hirofumi Hirai ◽  
Sawako Nakanishi ◽  
Tomoaki Nishida
Keyword(s):  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
White Rot
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