STUDIES ON THE TRANSPLANTATION OF A RAT TUMOR TO MICE

1957 ◽  
Vol 35 (1) ◽  
pp. 923-928
Author(s):  
A. Cameron Wallace ◽  
Susan Davis
Keyword(s):  
Tissue Culture ◽  
Mouse Brain ◽  
Serial Passage ◽  
Selective Adaptation ◽  
Growth And Survival ◽  
Heterologous Transplantation ◽  
Walker 256 ◽  
Foreign Species ◽  
Rat Tumor

The growth and survival of Walker 256 rat tumor grafted direct from rat into mouse thigh was compared with that of the same tumor after long continued residence in mouse brain, and also with that of tumors cultivated for a prolonged period in vitro. It was found that the pattern of growth and regression with tumor grown for 8 months in tissue culture was identical with that produced with tumor direct from rats. Tumor previously grown for 30 generations in mouse brain showed poorer growth and earlier regression. Since both prolonged growth in vitro and serial passage in mouse brain should have removed all the original rat stroma, it is concluded that loss of foreign stroma does not appreciably affect heterologous transplantation. Similarly, prolonged growth of this tumor in a foreign species under the conditions outlined apparently fails to produce any selective adaptation of the tumor to the host.

STUDIES ON THE TRANSPLANTATION OF A RAT TUMOR TO MICE

1957 ◽  
Vol 35 (11) ◽  
pp. 923-928
Author(s):  
A. Cameron Wallace ◽  
Susan Davis
Keyword(s):  
Tissue Culture ◽  
Mouse Brain ◽  
Serial Passage ◽  
Selective Adaptation ◽  
Growth And Survival ◽  
Heterologous Transplantation ◽  
Walker 256 ◽  
Foreign Species ◽  
Rat Tumor

The growth and survival of Walker 256 rat tumor grafted direct from rat into mouse thigh was compared with that of the same tumor after long continued residence in mouse brain, and also with that of tumors cultivated for a prolonged period in vitro. It was found that the pattern of growth and regression with tumor grown for 8 months in tissue culture was identical with that produced with tumor direct from rats. Tumor previously grown for 30 generations in mouse brain showed poorer growth and earlier regression. Since both prolonged growth in vitro and serial passage in mouse brain should have removed all the original rat stroma, it is concluded that loss of foreign stroma does not appreciably affect heterologous transplantation. Similarly, prolonged growth of this tumor in a foreign species under the conditions outlined apparently fails to produce any selective adaptation of the tumor to the host.

scholarly journals Effect of Serial Passage in Tissue Culture on Sequence of omp1 from Chlamydia trachomatisClinical Isolates

1998 ◽  
Vol 36 (12) ◽  
pp. 3686-3688 ◽  
Author(s):  
Diane R. Stothard ◽  
Barbara Van Der Pol ◽  
Nancy J. Smith ◽  
Robert B. Jones
Keyword(s):  
Tissue Culture ◽  
Chlamydia Trachomatis ◽  
Serial Passage ◽  
Epidemiological Studies ◽  
Clinical Specimens ◽  
Before And After

Previous epidemiological studies of Chlamydia trachomatis frequently have required expansion of isolates in tissue culture. The possibility that C. trachomatis omp1might undergo mutation during such expansion has not been examined systematically. We found no differences in the omp1sequences from 10 clinical specimens before and after 20 in vitro passages.

scholarly journals 077 In Vitro Growth of Immature Peach [Prunus persica (L.) Batsch] Embryos Related to Carbohydrate Source

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 401F-402
Author(s):  
Jonathan W. Sinclair ◽  
David H. Byrne
Keyword(s):  
Energy Source ◽  
Tissue Culture ◽  
Prunus Persica ◽  
Culture Media ◽  
Carbohydrate Source ◽  
Growth And Survival ◽  
Tissue Culture Media ◽  
Germination And Growth ◽  
Embryo Maturity

Carbohydrate energy source of various tissue culture media has an effect on growth and survival of the explants. Sucrose is the standard carbohydrate used in most tissue culture systems. The objective of the study was to determine the effect of five carbohydrate sources (fructose, glucose, maltose, sorbitol, and sucrose) at two levels (2% and 3%) on germination, growth, and survival of immature peach embryos (9.7 to 14.7mm) in vitro. Five cultivars were used. Overall, fructose, maltose, and sucrose each stimulated germination and growth as the primary carbohydrate energy source of peach embryo culture to the same degree; glucose and sorbitol were inferior. However, fructose was superior to sucrose in one cultivar. In general, sugar level did not affect survival, although cultivars did vary somewhat. Survival was found to be highly dependent upon embryo maturity.

RAPID IN VITRO PROPAGATION OF WESTERN GHATS CULTIVAR - COLOCASIA ESCULENTA FOR THE HIGH FREQUENCY OF PLANTLETS

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Jyothi R ◽  
Srinivasa Murthy K M ◽  
Hossein . ◽  
Veena .
Keyword(s):  
Tissue Culture ◽  
Wide Distribution ◽  
Colocasia Esculenta ◽  
Limiting Factor ◽  
Promising Alternative ◽  
Rapid Multiplication ◽  
Medical Plant ◽  
Total Fat ◽  
Planting Materials

Colocasia esculenta is commonly known as Taro, it is referred to as cocoyam in Nigeria. They are cherished for their rich taste, nutritional and medicinal properties. Every 100 g of taro corms possess 112 Kcal, 26.46 g carbohydrate, 1.50 g protein, 0.20 g total fat and 4.1g fiber (USDA National Nutrient Data Base). Besides its nutritional value, taro is used as a medical plant and provides bioactive compounds used as an anti-cancer drugs. Traditionally, cocoyams are vegetative propagated from tuber fragments, a practice that encourages pathogen distribution. Colocasia esculenta is a widely distributed food crop in the humid tropics and subtropics. Despite of its wide distribution, Taro plants are commonly infected with DsMV and other pathogens. This virus induces conspicuous mosaic, malformation, dwarfing or feathering on leaves in taro. As the results of infection, it reduces the quality and yield of taro production greatly. This virus is thus considered as a major limiting factor in the production of taro. Here plays the importance of  tissue culture plays a major role in producing the disease resistant plants round the year with high quality. For rapid multiplication and production of quality planting materials, tissue culture technology offers promising alternative compared to the traditional production methods. KEYWORDS: Colocasia esculenta, Virus, Pathogens, Conventional propagation, Micropropagation, Yield, Rapid multiplication, Quality

STUDIES ON THE GROWTH OF EXPERIMENTAL OVARIAN TUMOURS IN TISSUE CULTURE

1959 ◽  
Vol XXXII (I) ◽  
pp. 41-53 ◽  
Author(s):  
Stig Kullander ◽  
Bengt Källén
Keyword(s):  
Tissue Culture ◽  
Granulosa Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ovarian Tumours

ABSTRACT An in vitro study has been made of experimentally produced rat ovarian tumours of different age, paying particular attention to tumour reaction to crystallized steroids. Tumours of two histological structures were found: granulosa cell – luteoma tumours and arrhenoblastoma tumours. Both types grew in vitro and pictures of their cell appearance are given. The former type gave the best growth, and the endocrine studies were restricted to this type. The steroids tested (androsterone, oestrone, progesterone) all had an arresting effect in certain cases. This effect is not an unspecific, toxic one. The different tumours react to different extents, some being completely unaffected.

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

Standardization of in vitro nervous tissue culture for honeybee: A high specificity toxicological approach

2020 ◽  
Vol 189 ◽  
pp. 110040 ◽  
Author(s):  
Patricia Azevedo ◽  
Nicole Pavan Butolo ◽  
Luciano Delmondes de Alencar ◽  
Hellen Maria Soares-Lima ◽  
Victor Ribeiro Sales ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Nervous Tissue ◽  
High Specificity ◽  
Nervous Tissue Culture

scholarly journals Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture

2021 ◽  
Vol 28 (1) ◽  
Author(s):  
Renata Orłowska
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Taguchi Method ◽  
Culture Conditions ◽  
Dna Demethylation ◽  
Silver Ion ◽  
Donor Plant ◽  
In Vitro Tissue Culture ◽  
Induced Variation

Abstract Background Somatic embryogenesis is a phenomenon carried out in an environment that generates abiotic stress. Thus, regenerants may differ from the source of explants at the morphological, genetic, and epigenetic levels. The DNA changes may be the outcome of induction media ingredients (i.e., copper and silver ions) and their concentrations and time of in vitro cultures. Results This study optimised the level of copper and silver ion concentration in culture media parallel with the induction medium longevity step towards obtaining barley regenerants via somatic embryogenesis with a minimum or maximum level of tissue culture-induced differences between the donor plant and its regenerants. The optimisation process is based on tissue culture-induced variation evaluated via the metAFLP approach for regenerants derived under varying in vitro tissue culture conditions and exploited by the Taguchi method. In the optimisation and verification experiments, various copper and silver ion concentrations and the different number of days differentiated the tested trials concerning the tissue culture-induced variation level, DNA demethylation, and de novo methylation, including symmetric (CG, CHG) and asymmetric (CHH) DNA sequence contexts. Verification of optimised conditions towards obtaining regenerants with minimum and maximum variability compared to donor plants proved useful. The main changes that discriminate optimised conditions belonged to DNA demethylation events with particular stress on CHG context. Conclusions The combination of tissue culture-induced variation evaluated for eight experimental trials and implementation of the Taguchi method allowed the optimisation of the in vitro tissue culture conditions towards the minimum and maximum differences between a source of tissue explants (donor plant) and its regenerants from somatic embryos. The tissue culture-induced variation characteristic is mostly affected by demethylation with preferences towards CHG sequence context.

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