scholarly journals Red blood cell and white blood cell counts in sandpipers (Philomachus pugnax, Calidris canutus): effects of captivity, season, nutritional status, and frequent bleedings

2000 ◽  
Vol 78 (8) ◽  
pp. 1349-1355 ◽  
Author(s):  
Theunis Piersma ◽  
Anita Koolhaas ◽  
Anne Dekinga ◽  
Eberhard Gwinner

Captive ruffs (Philomachus pugnax) and red knots (Calidris canutus) kept in small flocks in outdoor aviaries maintained body mass and plumage cycles resembling those of free-living conspecifics. The persistence of identifiable annual cycles enabled us to study variability in two blood parameters, one a measure of red blood cell count (hematocrit) and the other an index of white blood cell (WBC) abundance (percentage). In both species hematocrit values averaged 0.43, somewhat lower than those measured in free-living red knots. Hematocrit varied little with time of year and we were unable to convincingly confirm predicted elevated hematocrit levels during periods of storage of fat for migration. In both species the percentage of WBCs (percent WBCs) initially declined from 0.6 to 0.8%; levels stabilized at 0.4% in ruffs and 0.3% in red knots after half a year in captivity. Using observations of the same individual red knots in the years before and after the experimental year as controls, biweekly extraction of about 30% of blood volume did not negatively affect seasonal changes in body mass and moult and breeding-plumage cycles. In nutritionally stressed red knots, hematocrit levels were low, and in these birds only, the small wounds inflicted by bleeding healed with difficulty.

2011 ◽  
Vol 9 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Marcos Tavares-Dias ◽  
Adriana Maria de Castro Monteiro ◽  
Elizabeth Gusmão Affonso ◽  
Kárlia Dalla Santa Amaral

Farming of native carnivore fish species has drawn attention due to their promising use in aquaculture. Among these species, tucunaré of the genus Cichla stand out, them being of high economical interest for sport fishing and Amazon's industry of ornamental fish. The present study describes the weight-length relationship (WLR), relative condition factor (Kn), red blood cell parameters, thrombocytes and leukocytes count of Cichla temensis Humboldt, 1821, farmed in central Amazon. Fish that underwent food training during fingerling culture received extruded ration containing 45% of crude protein during fattening, and had Kn with values from 0.925-1.199, which indicated good health condition during the culture. The equation obtained from the WLR was W = 0.0073Lt3.1435, indicating an isometric growth, which is the desirable for fish of fish farm. Red blood cell counts, total thrombocyte and leukocyte counts, hematocrit, hemoglobin concentration, mean corpuscular volume (MCV), concentration of mean corpuscular hemoglobin (MCHC), lymphocytes, monocytes and neutrophils had intra-specific variation. A significant (p<0.001) positive correlation of the red blood cells number with the hemoglobin concentration and hematocrit was found. These are the first sets of blood parameters for C. temensis and could be used as reference for comparison in further studies to evaluate the health status of this fish in different environments, because assessment of these parameters may be used as quick tool for diagnosing diseases, stress and malnutrition.


2016 ◽  
Vol 22 (2) ◽  
pp. 176-185
Author(s):  
Suzanne Smith ◽  
Phophi Madzivhandila ◽  
René Sewart ◽  
Ureshnie Govender ◽  
Holger Becker ◽  
...  

Disposable, low-cost microfluidic cartridges for automated blood cell counting applications are presented in this article. The need for point-of-care medical diagnostic tools is evident, particularly in low-resource and rural settings, and a full blood count is often the first step in patient diagnosis. Total white and red blood cell counts have been implemented toward a full blood count, using microfluidic cartridges with automated sample introduction and processing steps for visual microscopy cell counting to be performed. The functional steps within the microfluidic cartridge as well as the surrounding instrumentation required to control and test the cartridges in an automated fashion are described. The results recorded from 10 white blood cell and 10 red blood cell counting cartridges are presented and compare well with the results obtained from the accepted gold-standard flow cytometry method performed at pathology laboratories. Comparisons were also made using manual methods of blood cell counting using a hemocytometer, as well as a commercially available point-of-care white blood cell counting system. The functionality of the blood cell counting microfluidic cartridges can be extended to platelet counting and potential hemoglobin analysis, toward the implementation of an automated, point-of-care full blood count.


2015 ◽  
Vol 174 (8) ◽  
pp. 999-1014 ◽  
Author(s):  
Fátima Pérez-de-Heredia ◽  
◽  
Sonia Gómez-Martínez ◽  
Ligia-Esperanza Díaz ◽  
Ana M. Veses ◽  
...  

2014 ◽  
Vol 83 (2) ◽  
pp. 145-149 ◽  
Author(s):  
Sheng Jiang ◽  
Hong-Gang Fan ◽  
De-Zhang Lu ◽  
Jin-Long Hou ◽  
Xu-Dong Song ◽  
...  

Oxidative stress in the body occurs when the production of free radicals overwhelms the antioxidant defence systems. The aim of this study was to examine the effects of a tiletamine/zolazepam-xylazine-tramadol combination on the antioxidant status, lipoperoxidase and blood cell indicators in eight miniature pigs. Baseline measures were recorded prior to anaesthesia, then the animals were anaesthetized with the combination of tiletamine/zolazepam (3.5 mg/kg), xylazine (1.32 mg/kg), and tramadol (1.8 mg/kg). Blood samples were collected from the anterior vena cava at 15, 30, 60, 90, 120 min, 24 h, and 72 h after anaesthesia. Plasma malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase concentrations were measured by colorimetry, and red blood cell counts, white blood cell counts, haemoglobin and packed cell volume were determined using an automated cell counter. The results showed that the concentration of malondialdehyde increased significantly at 30 and 60 min after the injection (P< 0.05), whereas glutathione peroxidase and catalase activity increased slightly (P> 0.05) then returned to baseline values after 90 min (P> 0.05). Superoxide dismutase activity increased significantly (P< 0.05) at 30 and 60 min, and then gradually decreased to baseline values after 90 min. Changes in red blood cell counts, haemoglobin and packed cell volume were not significant, while white blood cell count decreased significantly (P< 0.05) at 30 and 60 min. Our study is the first to demonstrate that tiletamine/zolazepam-xylazine-tramadol provide antioxidant effects, which may be proposed for alleviating the stress of examination and research at veterinary clinics or long-distance transportation.


2020 ◽  
Vol 17 (1) ◽  
pp. 7-11
Author(s):  
Taiwo Kamardeen Bello ◽  
Balkisu Banke Oyelowo ◽  
Raheela Hassan ◽  
Brenda Oke ◽  
Sheriff Yusuf Idris ◽  
...  

The effects of graded levels of dietary protein on some haematological parameters in pubertal Yankasa rams were studied. They were randomly divided into three treatment groups (A, B and C). Groups A, B and C received 10%, 15% and 20% crude protein respectively. All rams were fed a basal diet of hay (Digitaria spp) ad-libitum and given a supplement ration of concentrate mixture at 2% body weight/day. Blood was collected once weekly via jugular venepuncture into heparinised test-tubes, and transported to the laboratory for determination of red blood cell counts, packed cell volume, white blood cell counts and total protein for twelve weeks. The overall mean red blood cell values obtained were 4.2 ± 0.1 ×106 µl, 5.2 ± 0.1 106 µl and 5.06 ± 0.1 ×106 µl, for groups A, B and C respectively. The difference between values obtained for groups B and C were not significantly different (P > 0.05). Mean packed cell volumes obtained in this study revealed that rams fed 15% crude protein had significantly (P < 0.05) higher values (31.3 ± 0.9%) than those fed 10% (25.9± 0.7%) but similar to those fed 20% (30.33 ± 0.56%) crude protein. There were no significant (P > 0.05) differences in mean white blood cell counts and total protein concentrations between all groups of animals fed 10% CP. Feeding rams diets containing 15 % CP (cotton seed cake and palm kernel cake) gave better values in terms of packed cell volume and red blood cell counts, than feeding diets containing 10% and 20% C.P. While graded levels of dietary protein was shown to affect packed cell volume and total protein concentration, it had no significant effect on red blood cell and white blood cell counts.  


2015 ◽  
Vol 29 (6) ◽  
pp. 823-829 ◽  
Author(s):  
Jundong Tian ◽  
Alexandre Courtiol ◽  
Karin Schneeberger ◽  
Alex D. Greenwood ◽  
Gábor Á. Czirják

Diabetes ◽  
2018 ◽  
Vol 67 (11) ◽  
pp. 2329-2336 ◽  
Author(s):  
Falastin Salami ◽  
Hye-Seung Lee ◽  
Eva Freyhult ◽  
Helena Elding Larsson ◽  
Åke Lernmark ◽  
...  

2021 ◽  
Vol 23 (1) ◽  
pp. 94-97
Author(s):  
F. U. C. MMEREOLE

An investigation was conducted to study the haematological parameters of the Nigerian local chicken using a total of 100 local chickens out of which 30 were randomly selected for bleeding. The blood samples were subjected to laboratory analysis to identify their haemoglobin contents, fragility and sedimentation rates as well as red blood cell counts, white blood cell counts and coagulation time. The results indicated that haemoglobin content ranged from 63.82 - 78.,43% with a mean of 70,43%, fragility and sedimentation rates ranged from 0.40 - 0.85% osmotic equivalent, coagulation time from 3.82 -6.25 minutes with a mean of 4.61 minutes, the red blood cell counts from 1.93 - 2.96 (x10°/ul) with a mean of 2.5 (x10°/ul) and white blood cell counts from 962 - 1,868 cells/ul with a mean of 1,216 cells/ul. It is believed that the establishment of the normal blood characteristics of the local chicken as done in this study, can be of immense aid in detecting abnormalities in the blood characteristics which can be used in diagnosing certain blood related diseases.


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