scholarly journals Biological identification of springtails (Hexapoda: Collembola) from the Canadian Arctic, using mitochondrial DNA barcodes

2004 ◽  
Vol 82 (5) ◽  
pp. 749-754 ◽  
Author(s):  
Ian D Hogg ◽  
Paul D.N Hebert
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C Oxidase ◽  
Sibling Species ◽  
Canadian Arctic ◽  
The Arctic ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
Cytochrome C Oxidase I ◽  
Morphological Taxonomy ◽  
Biological Identification

We evaluated sequence diversity in the mitochondrial cytochrome-c oxidase I (COI; EC 1.9.3.1) gene as a tool for resolving differences among species of Arctic springtails. The Collembola examined in this analysis were collected from Igloolik, Cornwallis, and Somerset islands and included representatives from all major families found in the Arctic. Members of 13 genera and 19 species were examined, including 4 species of the genus Folsomia and 3 species of the genus Hypogastrura. In all cases, species were successfully discriminated. Sequence divergences within species were generally less than 1%, whereas divergences between species were greater than 8% in all cases. Divergences among individuals of one species of Folsomia were much higher (up to 13%), but this likely represents the presence of an undescribed sibling species. We conclude that DNA barcoding is a powerful tool for identifying species of Collembola and should regularly be useful as a complement to traditional, morphological taxonomy.

scholarly journals Occurrence and molecular identification of an invasive rice strain of fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) from Sindh, Pakistan, using mitochondrial cytochrome c oxidase I gene sequences

2021 ◽  
Author(s):  
Sumaira Yousaf ◽  
Abdul Rehman ◽  
Mariyam Masood ◽  
Kazam Ali ◽  
Nazia Suleman
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Spodoptera Frugiperda ◽  
Fall Armyworm ◽  
Coi Gene ◽  
Invasive Pest ◽  
Mitochondrial Cytochrome ◽  
Cytochrome C Oxidase I ◽  
Gene Sequences ◽  
Rice Strain

AbstractThe fall armyworm (FAW), an invasive pest of maize, is an emerging threat in Southern Asia after America and Europe. Recently, this notorious pest has also been found in different areas of Pakistan. To assess its presence in Pakistan, a survey was carried out in the provinces of Punjab, Sindh, and Khyber Pakhtunkhwa during May–October 2019. We observed the highest incidence of FAW in Sindh with maximum impact in districts Tando-Allahyar and Hyderabad. These samples were identified as Spodoptera frugiperda on the morphological and taxonomical bases. However, morphological identification of this pest is very difficult at early larval instars. Here, we use the mitochondrial cytochrome c oxidase I (COI) gene region for the precise identification of larva of this invasive pest at species level. Two different regions of COI gene (COI-5′ and COI-3′) were used as molecular markers for the identification of this species. DNA sequence similarity searches of the obtained COI gene sequences (NCBI GenBank Accession Nos. MW241537, MW241538, MW349515, MW349516, MW349493 and MW349494) revealed that genetically it is more than 99% identical to S. frugiperda. The phylogenetic analysis indicated it as the rice-strain (R-strain). Both 3′- and 5′-fragment tree topologies showed that the collected samples of the FAW species belong to the R-strain. To the best of our knowledge, this is the first report providing molecular evidence for the existence of R-strain of S. frugiperda that was found feeding on maize crop in Sindh, Pakistan, using COI gene sequences as a marker.

First report on sensitivity of Caligus elongatus towards anti-louse chemicals and identification of mitochondrial cytochrome C oxidase I genotypes

Aquaculture ◽  
2019 ◽  
Vol 507 ◽  
pp. 190-195 ◽  
Author(s):  
Celia Agusti-Ridaura ◽  
Lars A. Hamre ◽  
Per G. Espedal ◽  
Øivind Øines ◽  
Tor E. Horsberg ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Mitochondrial Cytochrome ◽  
Cytochrome C Oxidase I ◽  
First Report ◽  
Mitochondrial Cytochrome C

Two new feather mites (Acari: Analgoidea) isolated from the grey-headed woodpecker, Picus canus (Piciformes: Picidae) in Korea

2019 ◽  
Vol 24 (11) ◽  
pp. 2167-2183
Author(s):  
Yeong-deok Han ◽  
Sergey V. Mironov ◽  
Gi-sik Min
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
Feather Mites ◽  
Oxidase Subunit ◽  
Two New Species ◽  
First Time

Two new species of feather mites from the superfamily Analgoidea are described from the grey-headed woodpecker, Picus canus, in Korea: Neopteronyssus koreanus sp. nov. (Pteronyssidae) and Proterothrix picinus sp. nov. (Proctophyllodidae: Pterodectinae). Feather mites of the genera Neopteronyssus Mironov, 2002 and Proterothrix Gaud, 1968 are described for the first time in Korea. Morphological descriptions of both new species are complemented with partial sequences of their mitochondrial cytochrome c oxidase subunit I (COI) gene as DNA barcodes.

scholarly journals Novel Point Mutations and A8027G Polymorphism in Mitochondrial-DNA-Encoded Cytochrome c Oxidase II Gene in Mexican Patients with Probable Alzheimer Disease

2014 ◽  
Vol 2014 ◽  
pp. 1-5
Author(s):  
Verónica Loera-Castañeda ◽  
Lucila Sandoval-Ramírez ◽  
Fermín Paul Pacheco Moisés ◽  
Miguel Ángel Macías-Islas ◽  
Moisés Alejandro Alatorre Jiménez ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Alzheimer Disease ◽  
Cytochrome C Oxidase ◽  
Point Mutations ◽  
Mitochondrial Cytochrome ◽  
Catalytic Core ◽  
Oxidase Gene ◽  
Mexican Patients ◽  
Mitochondrial Cytochrome C

Mitochondrial dysfunction has been thought to contribute to Alzheimer disease (AD) pathogenesis through the accumulation of mitochondrial DNA mutations and net production of reactive oxygen species (ROS). Mitochondrial cytochrome c-oxidase plays a key role in the regulation of aerobic production of energy and is composed of 13 subunits. The 3 largest subunits (I, II, and III) forming the catalytic core are encoded by mitochondrial DNA. The aim of this work was to look for mutations in mitochondrial cytochrome c-oxidase gene II (MTCO II) in blood samples from probable AD Mexican patients.MTCO IIgene was sequenced in 33 patients with diagnosis of probable AD. Four patients (12%) harbored the A8027G polymorphism and three of them were early onset (EO) AD cases with familial history of the disease. In addition, other four patients with EOAD had only one of the following point mutations: A8003C, T8082C, C8201T, or G7603A. Neither of the point mutations found in this work has been described previously for AD patients, and the A8027G polymorphism has been described previously; however, it hasn’t been related to AD. We will need further investigation to demonstrate the role of the point mutations of mitochondrial DNA in the pathogenesis of AD.

Revision of the subgenus Phortica (sensu stricto) (Diptera, Drosophilidae) from East Asia, with assessment of species delimitation using DNA barcodes

Zootaxa ◽  
2019 ◽  
Vol 4678 (1) ◽  
pp. 1-75
Author(s):  
JIA HUANG ◽  
LU GONG ◽  
SHUN-CHERN TSAUR ◽  
LIN ZHU ◽  
KEYING AN ◽  
...  
Keyword(s):  
New Species ◽  
Cytochrome C Oxidase ◽  
Species Delimitation ◽  
Species Complex ◽  
Dna Barcode ◽  
Sensu Stricto ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
New Synonyms

A total of 50 (43 known and seven new) species in the subgenus Phortica (sensu stricto) were surveyed and (re)described from China: P. bicornuta (Chen & Toda, 1997); P. bipartita (Toda & Peng, 1992); P. biprotrusa (Chen & Toda, 1998); P. cardua (Okada, 1977); P. chi (Toda & Sidorenko, 1996); P. conifera (Okada, 1977); P. eparmata (Okada, 1977); P. eugamma (Toda & Peng, 1990); P. excrescentiosa (Toda & Peng, 1990); P. fangae (Máca, 1993); P. flexuosa (Zhang & Gan, 1986); P. foliata (Chen & Toda, 1997); P. gamma (Toda & Peng, 1990); P. gigas (Okada, 1977); P. glabtabula Chen & Gao, 2005; P. hainanensis (Chen & Toda, 1998); P. hongae (Máca, 1993); P. huazhii Cheng & Chen, 2008; P. iota (Toda & Sidorenko, 1996); P. jadete Zhu, Cao & Chen, 2018; P. kappa (Máca, 1977); P. lambda (Toda & Peng, 1990); P. latifoliacea Chen & Watabe, 2008; P. magna (Okada, 1960); P. okadai (Máca, 1977); P. omega (Okada, 1977); P. orientalis (Hendel, 1914); P. pangi Chen & Wen, 2005; P. paramagna (Okada, 1971); P. perforcipata (Máca & Lin, 1993); P. pi (Toda & Peng, 1990); P. protrusa (Zhang & Shi, 1997); P. pseudopi (Toda & Peng, 1990); P. pseudotau (Toda & Peng, 1990); P. psi (Zhang & Gan, 1986); P. rhagolobos Chen & Gao, 2008; P. saeta (Zhang & Gan, 1986); P. setitabula Chen & Gao, 2005; P. subradiata (Okada, 1977); P. tau (Toda & Peng, 1990); P. uncinata Chen & Gao, 2005; P. unipetala Chen & Wen, 2005; P. allomega Gong & Chen, sp. nov.; P. archikappa Gong & Chen, sp. nov.; P. dianzangensis Gong & Chen, sp. nov.; P. imbacilia Gong & Chen, sp. nov.; P. liukuni Gong & Chen, sp. nov.; P. tibeta Gong & Chen, sp. nov.; and P. xianfui Gong & Chen, sp. nov. In addition, seven new synonyms were recognized: P. acongruens (Zhang & Shi, 1997), syn. nov.; P. antillaria (Chen & Toda, 1997), syn. nov.; P. kukuanensis Máca, 2003, syn. nov.; P. linae (Máca & Chen, 1993), syn. nov.; P. shillongensis (Singh & Gupta, 1979), syn. nov.; P. takadai (Okada, 1977), syn. nov.; and P. watanabei (Máca & Lin, 1993), syn. nov. A key to all Asian species (except for the eparmata species complex) of this subgenus was provided. All currently available DNA barcode (partial mitochondrial cytochrome c oxidase subunit I (COI) gene) sequences of this subgenus (217 sequences of 54 species) are employed in a molecular analysis using different species delimitation methods. The results indicate that approximately 68.5% (37 of 54 spp.) of Phortica (s. str.) species could be clearly distinguished from closely related morphospecies or cryptic species. 

DNA-based identification of South Korean Megaloptera larvae with taxonomic notes

2015 ◽  
Vol 148 (2) ◽  
pp. 123-139 ◽  
Author(s):  
Sang Woo Jung ◽  
Tatyana S. Vshivkova ◽  
Yeon Jae Bae
Keyword(s):  
Mitochondrial Dna ◽  
Larval Stage ◽  
Adult Stage ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
Gene Sequences ◽  
Mtdna Sequences ◽  
Larval Stages ◽  
South Korean

AbstractMitochondrial DNA (mtDNA) sequences, which serve as DNA barcodes, have been used to associate immature and adult stages of insects and to delineate species. The partial mitochondrial cytochromecoxidase subunit I (COI) gene sequences for South Korean Megaloptera (all known in the adult stage) were tested to identify undetermined larvae as a rapid and effective method from 31 specimens (16 adults and 15 larvae). The COI gene sequences distinguished all six known Megaloptera species, with a low genetic distance between larvae and adults (0.50±0.21%). Based on the COI gene sequences, we associated five types of larvae with known adults including the following four species of newly described larval stages:Sialis annaeVshivkova, 1979 (Megaloptera: Sialidae);Neochauliodes formosanus(Okamoto, 1910) (Megaloptera: Corydalidae);Parachauliodes asahinaiLiuet al., 2008 (Megaloptera: Corydalidae); andProtohermes xanthodesNavás, 1913 (Megaloptera: Corydalidae). The known larval stage ofSialis koreanaJung and Bae, 2012 (Megaloptera: Sialidae) was confirmed, and the morphological variation in the male genitalia ofSialis longidensKlingstedt, 1932 (Megaloptera: Sialidae) is discussed. A larval key to the six South Korean species of Megaloptera is provided.

scholarly journals Molecular Phylogeny of Giant Clams Based on Mitochondrial DNA Cytochrome C Oxidase I Gene

2007 ◽  
Vol 14 (4) ◽  
pp. 162-166 ◽  
Author(s):  
AGUS NURYANTO ◽  
DEDY DURYADI ◽  
DEDI SOEDHARMA ◽  
DIETMAR BLOHM
Keyword(s):  
Mitochondrial Dna ◽  
Cytochrome C ◽  
Molecular Phylogeny ◽  
Cytochrome C Oxidase ◽  
Cytochrome C Oxidase I ◽  
Giant Clams ◽  
I Gene
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