A preliminary analysis of dietary effects on serum ninhydrin-positive compounds of immature lobsters Homarus americanus (Milne Edwards)

1978 ◽  
Vol 56 (2) ◽  
pp. 359-363 ◽  
Author(s):  
John A. Carter ◽  
Gary B. Dunphy
Keyword(s):  
Glutamic Acid ◽  
Butyric Acid ◽  
Preliminary Analysis ◽  
Urea Cycle ◽  
Homarus Americanus ◽  
Aminobutyric Acid ◽  
Cysteic Acid ◽  
Total Serum ◽  
Serum Concentrations ◽  
Apparent Increase

Thirty-eight ninhydrin-positive compounds (NPCs) were detected in the serum of wild, controlled-diet and starved lobsters Homarus americanus (Milne Edwards). Glycine, proline, alanine, taurine, serine, glutamic acid, ornithine, and glutamine comprised approximately 82% of the total serum NPC concentration in wild and starved lobsters and 70% in fed lobsters. The urea cycle intermediates arginine, citrulline, ornithine, and urea were detected in many samples. The following NPCs, previously unreported in lobster haemolymph, were detected as well: β-alanine, α-aminoadipic acid, α -amino-n-butyric acid, γ-aminobutyric acid, ammonia, anserine, as-paragine, cystathionine, cysteic acid – phosphoserine, ethanolamine, glycerophosphoethanol-amine, hydroxyproline, 1- and 3-methylhistidine. Starved lobsters showed pronounced reductions in serum NPC concentrations relative to those of fed lobsters. Starvation caused an apparent increase in the serum concentrations of the methylhistidine isomers and glycerophosphoethanolamine.

Novel Co-fermentation of Dendropanax morbifera Extract to Produce γ-aminobutyric Acid and Poly-γ-glutamic Acid

2019 ◽  
Vol 25 (6) ◽  
pp. 785-791
Author(s):  
Woong-Kyu Yoon ◽  
Jae-Won Choi ◽  
Jong-Soon Lim ◽  
Coralia V. Garcia ◽  
Sam-Pin Lee
Keyword(s):  
Glutamic Acid ◽  
Aminobutyric Acid ◽  
Dendropanax Morbifera

scholarly journals γ-AMINOBUTYRIC ACID IN BRAIN: ITS FORMATION FROM GLUTAMIC ACID

1950 ◽  
Vol 187 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Eugene Roberts ◽  
Sam Frankel
Keyword(s):  
Glutamic Acid ◽  
Aminobutyric Acid

Muscle Weight Relationship to Serum Proteins, Hemocytes, and Hepatopancreas in the Lobster, Homarus americanus

1967 ◽  
Vol 24 (11) ◽  
pp. 2339-2354 ◽  
Author(s):  
James E. Stewart ◽  
John W. Cornick ◽  
Diane M. Foley ◽  
M. F. Li ◽  
C. M. Bishop
Keyword(s):  
Serum Protein ◽  
Physiological Condition ◽  
Serum Proteins ◽  
Homarus Americanus ◽  
Total Serum Protein ◽  
Total Serum ◽  
Experimental Conditions ◽  
Muscle Weight ◽  
Beef Liver ◽  
The Relationship

Total serum protein values, hemocyte numbers, and muscle weights were determined for 216 intermolt lobsters immediately after their capture, and for 230 others held captive under a variety of dietary and environmental conditions. Average muscle values ranged from approximately 13% to the more normal 20–25% of the live animals' weight, depending upon experimental conditions. The total serum protein up to a level of 55 mg/ml was shown to be a reliable indicator of muscle weights, although the relationship was not identical for all lobster groups. It appeared to be modified chiefly by the areas from which the different groups were taken. Diet was more important than the temperatures (5 to 14 C) in affecting changes in muscle and serum protein values. Starvation caused a greater reduction (50 to 70%) in the size of the hepatopancreas than in the muscle. Histological examination of the hepatopancreatic tissue showed that the lipid content was markedly reduced upon starvation and that a degeneration of this organ was apparent for lobsters fed a beef liver and herring diet. Measurement of serum proteins would appear to be a useful technique in experiments on lobster nutrition and have value, within specified limits, for assessing the physiological condition of wild lobsters.

The preparation of γ-aminobutyric acid by enzymatic decarboxylation of l-glutamic acid

1953 ◽  
Vol 43 (2) ◽  
pp. 378-380 ◽  
Author(s):  
Merrill N. Camien ◽  
Lawrence E. McClure ◽  
Albert Lepp ◽  
Max S. Dunn
Keyword(s):  
Glutamic Acid ◽  
Aminobutyric Acid ◽  
Enzymatic Decarboxylation

scholarly journals Production of γ-Aminobutyric Acid in Pumpkin Tissue Treated with Freeze-thaw Infusion of L-Glutamic Acid Monosodium Salt

2013 ◽  
Vol 19 (4) ◽  
pp. 641-646 ◽  
Author(s):  
Yoshimi WATANABE ◽  
Kazuyasu MURAKAMI ◽  
Koji SAKAMOTO ◽  
Tomoko FUJIWARA ◽  
Akihiro TAI ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Aminobutyric Acid ◽  
Freeze Thaw ◽  
Monosodium Salt

Changes in amino acid compounds of wheat plants during ensiling and aerobic exposure: the influence of propionic acid and urea phosphate-calcium propionate

1984 ◽  
Vol 102 (3) ◽  
pp. 667-672 ◽  
Author(s):  
G. Ashbell ◽  
H. H. Theune ◽  
D. Sklan
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Propionic Acid ◽  
Butyric Acid ◽  
Total Amino Acid ◽  
Amino Butyric Acid ◽  
Urea Phosphate ◽  
Fresh Material ◽  
Calcium Propionate

SummaryChanges in distribution of amino acid nitrogen of chopped wheat plants ensiled at shooting and flowering when wilted, and at the milk and dough stages as fresh material, were determined as affected by addition of 0·8% propionic acid (PrA) or 2·2% urea phosphate-calcium propionate (UP-CaPr). Analyses were carried out after an ensiling period of 90 days and after a further aerobic exposure period (AE) of 7 days.Total amino acid (TAA) contents in the dry matter (D.M.) during the fermentation period and in the AE were stable in untreated material (UM) and treated material. Concentration of essential amino acids decreased during fermentation, this decrease being higher in the UM. The free amino acids were low in the fresh material (18·6% of TAA) but increased in the ensiled material to ca. 71 % of the TAA in the silage. In the AE this level was 63% in UM and 69% in treated material. The ammonia-N contents increased during fermentation in UM and especially in the UP-CaPr treatments, while the opposite occurred in the PrA treatments.The concentrations of and changes in 21 amino acids (AAs) are given. The highest AA concentrations recorded in the fresh material were those of arginine, lysine, glutamic acid, alanine, leucine, proline and glycine. The most marked increments in AAs as a result of fermentation were those of ornithine, γ-amino butyric acid, threonine and methionine. Marked decreases were observed in glutamine, arginine and glutamic acid. PrA increased mainly arginine, asparagine and glutamine, whereas γ-amino butyric acid decreased; UP-CaPr increased arginine, asparagine, lysine and glutamic acid (in silage only) and reduced γ-amino butyric acid and glutamine (in AE only).

Vancomycin Serum Protein Binding Determination by Ultrafiltration

1988 ◽  
Vol 22 (4) ◽  
pp. 300-303 ◽  
Author(s):  
Bruce H. Ackerman ◽  
E. Howard Taylor ◽  
Keith M. Olsen ◽  
Wedad Abdel-Malak ◽  
Alexandras A. Pappas
Keyword(s):  
Protein Binding ◽  
Correlation Coefficient ◽  
Pharmacokinetic Study ◽  
Room Temperature ◽  
Total Serum ◽  
Serum Protein Binding ◽  
Serum Concentrations ◽  
Orthogonal Regression ◽  
The Mean ◽  
Serum Vancomycin

Sixty-two serum concentrations were obtained from 12 infected patients enrolled in a vancomycin pharmacokinetic study. Both unbound and total serum vancomycin concentrations were measured using ultrafiltration and a commercial fluorescent polarization immunoassay. Ultrafiltrates were obtained by centrifugation at 1000 × g for ten minutes at room temperature and their assay indicated a range in protein binding from 7.9 to 71 percent. The mean protein binding (mean ±SD) was 41.95 ± 14.15 percent. No measurable adsorption of vancomycin onto the ultrafiltration membrane was noted. Orthogonal regression of unbound versus total vancomycin concentrations was described by the equation y=0.597x-0.362 with a correlation coefficient of 0.948.

scholarly journals Serum proteinogram of the Campeiro horse

2019 ◽  
Vol 71 (2) ◽  
pp. 363-368
Author(s):  
A.F. Souza ◽  
J. Schade ◽  
A.F. Ramos ◽  
M.S.M. Albuquerque ◽  
G.V. Fonteque ◽  
...  
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Reference Interval ◽  
Total Serum Protein ◽  
Total Serum ◽  
Serum Concentrations ◽  
Reproductive Condition ◽  
Serum Fractions ◽  
Pregnancy Status ◽  
Biuret Method

ABSTRACT The aim of this study to measure the fractions of the total serum proteins of the Campeiro horse and identify the influences of biological variants. Blood samples were taken in 138 horses of the breed Campeiro for measuring the concentration of total serum protein by the biuret method. Serum concentrations of protein fractions were measured by electrophoresis using agarose gel. Groups were formed according to age, sex and reproductive condition. The average values of serum fractions: albumin (2.85±0.36g/dl), alpha 1 (0.28±0.11g/dl), alpha 2 (0.26±0.08g/dL) beta 1 (0.57±0.15g/dl), beta 2 (0.89±0.28g/dL), gamaglobulinas (1.86±0.34g/dL), albumin/globulin ratio (0.75±0.18) and 2.5% percentile and 97.5% had slight differences in relation to the reference interval proposed for the species. They observed higher values of alpha 1 and 2 globulins in the group from that had six to eight years old and gammaglobulins in group above 13 years old. Serum protein concentrations were similar in horses and mares and between non-pregnant and pregnant. Sex and pregnancy status did not affect serum proteinogram. Alpha and gammaglobulins have higher values as the age increases. Serum proteinogram of Campeiro horses shows variations that have to be considered in the interpretation of laboratory tests.

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