Modelling ocean acidification effects with life stage-specific responses alters spatiotemporal patterns of catch and revenues of American lobster, Homarus americanus

Ocean acidification (OA) affects marine organisms through various physiological and biological processes, yet our understanding of how these translate to large-scale population effects remains limited. Here, we integrated laboratory-based experimental results on the life history and physiological responses to OA of the American lobster, Homarus americanus, into a dynamic bioclimatic envelope model to project future climate change effects on species distribution, abundance, and fisheries catch potential. Ocean acidification effects on juvenile stages had the largest stage-specific impacts on the population, while cumulative effects across life stages significantly exerted the greatest impacts, albeit quite minimal. Reducing fishing pressure leads to overall increases in population abundance while setting minimum size limits also results in more higher-priced market-sized lobsters (> 1 lb), and could help mitigate the negative impacts of OA and concurrent stressors (warming, deoxygenation). However, the magnitude of increased effects of climate change overweighs any moderate population gains made by changes in fishing pressure and size limits, reinforcing that reducing greenhouse gas emissions is most pressing and that climate-adaptive fisheries management is necessary as a secondary role to ensure population resiliency. We suggest possible strategies to mitigate impacts by preserving important population demographics.

Spatial and temporal patterns in the sex ratio of American lobsters (Homarus americanus) in southwestern Nova Scotia, Canada

An approximate 1:1 sex ratio of American lobsters can be skewed due to environmental factors or fisheries management. Substantial skewness can impact mating behaviour and lower reproduction which could have far-reaching ecological and economic consequences. The aim was to investigate the sex ratio patterns of lobsters in two lobster fishing areas (LFAs) in southwestern Nova Scotia, Canada and identify factors associated with skewed sex ratios. This study analyzed biological data from more than 270,000 lobsters sampled over ten years (2010–2019) by the Fishermen and Scientists Research Society. A mixed effect logistic regression model evaluated the effect of spatial, temporal and environmental factors as well as size on the sex ratio of lobsters. There were significant temporal patterns in sex ratios that differed by LFA. After the effects of sampling month, year and LFA were accounted for, lower bottom temperature and deeper water depth were associated with a higher prevalence of females, especially in larger lobsters. We present the first long term analyses of sex ratio patterns in H. americanus in Atlantic Canada’s most commercially important region for this species and provide evidence that these patterns are influenced by environmental factors and fisheries. In view of future climate change scenarios, monitoring the population dynamics of this iconic fishery species is crucial to ensure sustainable fisheries and healthy lobster stocks.

Tolerant Larvae and Sensitive Juveniles: Integrating Metabolomics and Whole-Organism Responses to Define Life-Stage Specific Sensitivity to Ocean Acidification in the American Lobster

Bentho-pelagic life cycles are the dominant reproductive strategy in marine invertebrates, providing great dispersal ability, access to different resources, and the opportunity to settle in suitable habitats upon the trigger of environmental cues at key developmental moments. However, free-dispersing larvae can be highly sensitive to environmental changes. Among these, the magnitude and the occurrence of elevated carbon dioxide (CO2) concentrations in oceanic habitats is predicted to exacerbate over the next decades, particularly in coastal areas, reaching levels beyond those historically experienced by most marine organisms. Here, we aimed to determine the sensitivity to elevated pCO2 of successive life stages of a marine invertebrate species with a bentho-pelagic life cycle, exposed continuously during its early ontogeny, whilst providing in-depth insights on their metabolic responses. We selected, as an ideal study species, the American lobster Homarus americanus, and investigated life history traits, whole-organism physiology, and metabolomic fingerprints from larval stage I to juvenile stage V exposed to different pCO2 levels. Current and future ocean acidification scenarios were tested, as well as extreme high pCO2/low pH conditions that are predicted to occur in coastal benthic habitats and with leakages from underwater carbon capture storage (CCS) sites. Larvae demonstrated greater tolerance to elevated pCO2, showing no significant changes in survival, developmental time, morphology, and mineralisation, although they underwent intense metabolomic reprogramming. Conversely, juveniles showed the inverse pattern, with a reduction in survival and an increase in development time at the highest pCO2 levels tested, with no indication of metabolomic reprogramming. Metabolomic sensitivity to elevated pCO2 increased until metamorphosis (between larval and juvenile stages) and decreased afterward, suggesting this transition as a metabolic keystone for marine invertebrates with complex life cycles.

Newly Hatched Stage I American Lobster (Homarus americanus) Survival Following Exposure to Physically and Chemically Dispersed Crude Oil

Standard model species are commonly used in toxicity tests due to their biological and technical advantages but studying native species increases the specificity and relevance of results generated for the potential risk assessment to an ecosystem. Accounting for intraspecies variability and other factors, such as chemical and physical characterization of test medium, is necessary to develop a reproducible bioassay for toxicity testing with native species. In this study, larval stage I American lobster (Homarus americanus) was selected as the test species, which is native to Atlantic Canada. Toxicity tests were first conducted exposing lobster larvae to a reference toxicant of copper sulfate (CuSO4) and then to physically and chemically dispersed oil. The effect on larval survival was estimated by calculating the median effect concentration (EC50) as 2.54-9.73 mg TPH/L when all trials are considered together. The HC5 or PNEC value was 2.52 mg TPH/L and therefore a narrow difference from the EC50 value. The inter-trial variability (coefficient of variability = 17%) was lower than the US Environmental Protection Agency standard test species of mysid shrimp (Americamysis bahia) and inland silversides (Menidia bervillina). Our results indicate that the described larval lobster bioassay is reliable to produce repeatable results for this commercially important and native species of Atlantic Canada.

Does Differential Receptor Distribution Underlie Variable Responses to a Neuropeptide in the Lobster Cardiac System?

Central pattern generators produce rhythmic behaviors independently of sensory input; however, their outputs can be modulated by neuropeptides, thereby allowing for functional flexibility. We investigated the effects of C-type allatostatins (AST-C) on the cardiac ganglion (CG), which is the central pattern generator that controls the heart of the American lobster, Homarus americanus, to identify the biological mechanism underlying the significant variability in individual responses to AST-C. We proposed that the presence of multiple receptors, and thus differential receptor distribution, was at least partly responsible for this observed variability. Using transcriptome mining and PCR-based cloning, we identified four AST-C receptors (ASTCRs) in the CG; we then characterized their cellular localization, binding potential, and functional activation. Only two of the four receptors, ASTCR1 and ASTCR2, were fully functional GPCRs that targeted to the cell surface and were activated by AST-C peptides in our insect cell expression system. All four, however, were amplified from CG cDNAs. Following the confirmation of ASTCR expression, we used physiological and bioinformatic techniques to correlate receptor expression with cardiac responses to AST-C across individuals. Expression of ASTCR1 in the CG showed a negative correlation with increasing contraction amplitude in response to AST-C perfusion through the lobster heart, suggesting that the differential expression of ASTCRs within the CG is partly responsible for the specific physiological response to AST-C exhibited by a given individual lobster.

Examining Scale Dependent Environmental Effects on American Lobster (Homarus americanus) Spatial Distribution in a Changing Gulf of Maine

The Gulf of Maine (GOM) is a highly complex environment and previous studies have suggested the need to account for spatial nonstationarity in species distribution models (SDMs) for the American lobster (Homarus americanus). To explore impacts of spatial nonstationarity on species distribution, we compared models with the following three assumptions : (1) large-scale and stationary relationships between species distributions and environmental variables; (2) meso-scale models where estimated relationships differ between eastern and western GOM, and (3) finer-scale models where estimated relationships vary across eastern, central, and western regions of the GOM. The spatial scales used in these models were largely determined by the GOM coastal currents. Lobster data were sourced from the Maine-New Hampshire Inshore Bottom Trawl Survey from years 2000–2019. We considered spatial and environmental variables including latitude and longitude, bottom temperature, bottom salinity, distance from shore, and sediment grain size in the study. We forecasted distributions for the period 2028–2055 using each of these models under the Representative Concentration Pathway (RCP) 8.5 “business as usual” climate warming scenario. We found that the model with the third assumption (i.e., finest scale) performed best. This suggests that accounting for spatial nonstationarity in the GOM leads to improved distribution estimates. Large-scale models revealed a tendency to estimate global relationships that better represented a specific location within the study area, rather than estimating relationships appropriate across all spatial areas. Forecasted distributions revealed that the largest scale models tended to comparatively overestimate most season × sex × size group lobster abundances in western GOM, underestimate in the western portion of central GOM, and overestimate in the eastern portion of central GOM, with slightly less consistent and patchy trends amongst groups in eastern GOM. The differences between model estimates were greatest between the largest and finest scale models, suggesting that fine-scale models may be useful for capturing effects of unique dependencies that may operate at localized scales. We demonstrate how estimates of season-, sex-, and size- specific American lobster spatial distribution would vary based on the spatial scale assumption of nonstationarity in the GOM. This information may help develop appropriate local adaptation measures in a region that is susceptible to climate change.

G protein-coupled receptors as candidates for modulation and activation of the chemical senses in decapod crustaceans

Many studies have characterized class A GPCRs in crustaceans; however, their expression in crustacean chemosensory organs has yet to be detailed. Class A GPCRs comprise several subclasses mediating diverse functions. In this study, using sequence homology, we classified all putative class A GPCRs in two chemosensory organs (antennular lateral flagellum [LF] and walking leg dactyls) and brain of four species of decapod crustaceans (Caribbean spiny lobster Panulirus argus, American lobster Homarus americanus, red-swamp crayfish Procambarus clarkii, and blue crab Callinectes sapidus). We identified 333 putative class A GPCRs– 83 from P. argus, 81 from H. americanus, 102 from P. clarkii, and 67 from C. sapidus–which belong to five distinct subclasses. The numbers of sequences for each subclass in the four decapod species are (in parentheses): opsins (19), small-molecule receptors including biogenic amine receptors (83), neuropeptide receptors (90), leucine-rich repeat-containing GPCRs (LGRs) (24), orphan receptors (117). Most class A GPCRs are predominately expressed in the brain; however, we identified multiple transcripts enriched in the LF and several in the dactyl. In total, we found 55 sequences with higher expression in the chemosensory organs relative to the brain across three decapod species. We also identified novel transcripts enriched in the LF including a metabotropic histamine receptor and numerous orphan receptors. Our work establishes expression patterns for class A GPCRs in the chemosensory organs of crustaceans, providing insight into molecular mechanisms mediating neurotransmission, neuromodulation, and possibly chemoreception.

The American lobster genome reveals insights on longevity, neural, and immune adaptations

The American lobster, Homarus americanus, is integral to marine ecosystems and supports an important commercial fishery. This iconic species also serves as a valuable model for deciphering neural networks controlling rhythmic motor patterns and olfaction. Here, we report a high-quality draft assembly of the H. americanus genome with 25,284 predicted gene models. Analysis of the neural gene complement revealed extraordinary development of the chemosensory machinery, including a profound diversification of ligand-gated ion channels and secretory molecules. The discovery of a novel class of chimeric receptors coupling pattern recognition and neurotransmitter binding suggests a deep integration between the neural and immune systems. A robust repertoire of genes involved in innate immunity, genome stability, cell survival, chemical defense, and cuticle formation represents a diversity of defense mechanisms essential to thrive in the benthic marine environment. Together, these unique evolutionary adaptations contribute to the longevity and ecological success of this long-lived benthic predator.