External morphology of the developing hemipenes of the dwarf chameleon, Bradypodion pumilum (Reptilia: Chamaeleonidae)

1989 ◽  
Vol 67 (4) ◽  
pp. 884-890 ◽  
Author(s):  
Herbert I. Rosenberg ◽  
Aaron M. Bauer ◽  
Anthony P. Russell
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sexual Maturity ◽  
Developmental Stages ◽  
Close Association ◽  
External Morphology ◽  
Squamate Reptiles ◽  
The Family ◽  
Scanning Electron

The external morphology of the hemipenes of squamate reptiles is often complex and has been widely employed in systematic studies. Little is known, however, of the development of these patterns and thus of the potential polarity of the character states used in systematic studies. Analysis of three developmental stages of the hemipenes of the chameleon Bradypodion pumilum using scanning electron microscopy reveals that the complexity of pattern emerges gradually. Initially the hemipenial anlagen appear as simple, external lobes that develop in close association with the cloaca. By stage 38 a shallow sulcus spermaticus is evident and calices have begun to appear. By stage 41 embryos possess a deep, protected sulcus and two pairs of distal lobes. These lobes will eventually differentiate into semicircular disks (rotulae) with serrated margins that are characteristic of many members of the family. The complex external morphology of the hemipenes that is of significance in systematic studies and of assumed importance in copulatory mechanics may not be expressed until sexual maturity is reached.

Comparative Development of Some Taxonomically Critical Floral Inflorescence Features in Cyperaceae

1991 ◽  
Vol 39 (2) ◽  
pp. 119 ◽  
Author(s):  
JJ Bruhl
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Developmental Stages ◽  
Floral Morphology ◽  
Developmental Studies ◽  
Comparative Development ◽  
Inflorescence Morphology ◽  
The Family ◽  
Scanning Electron

Morphology at different developmental stages was investigated by dissection and by scanning electron microscopy (SEM) in five sedges: Eleocharis (three species) and Schoenoplectus (both Cyperoideae, Scirpeae), and Lepidosperma (Caricoideae, Schoeneae). In each case all the perianth segments (scales or bristles) were positioned outside the staminal primordia or stamens, consistent with classical interpretations of flowers. Putative exceptions and previous alternative interpretations of floral morphology in the Cyperaceae are discussed. SEM developmental studies of Hypolytreae (e.g. Scirpodendron) are needed for further clarification of interpretative floral/inflorescence morphology in the family.

SEM analysis of fish scale cross sections: A technique study

1992 ◽  
Vol 50 (1) ◽  
pp. 744-745
Author(s):  
M.E. Lee ◽  
A. Moller ◽  
P.S.O. Fouche ◽  
I.G Gaigher
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cross Sections ◽  
Soft Tissues ◽  
Close Association ◽  
Sem Analysis ◽  
Fish Scale ◽  
Fish Scales ◽  
Micro Structures ◽  
Scanning Electron

Scanning electron microscopy of fish scales has facilitated the application of micro-structures to systematics. Electron microscopy studies have added more information on the structure of the scale and the associated cells, many problems still remain unsolved, because of our incomplete knowledge of the process of calcification. One of the main purposes of these studies has been to study the histology, histochemistry, and ultrastructure of both calcified and decalcified scales, and associated cells, and to obtain more information on the mechanism of calcification in the scales. The study of a calcified scale with the electron microscope is complicated by the difficulty in sectioning this material because of the close association of very hard tissue with very soft tissues. Sections often shatter and blemishes are difficult to avoid. Therefore the aim of this study is firstly to develop techniques for the preparation of cross sections of fish scales for scanning electron microscopy and secondly the application of these techniques for the determination of the structures and calcification of fish scales.

The External Morphology of the Eggs of Culex (Culex) saltanensis (Diptera: Culicidae) Under Scanning Electron Microscopy

2020 ◽  
Author(s):  
J R Santos-Mallet ◽  
T D Balthazar ◽  
A A Oliveira ◽  
W A Marques ◽  
A Q Bastos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Optical Microscopy ◽  
Osmium Tetroxide ◽  
Neotropical Region ◽  
External Morphology ◽  
Metal Supports ◽  
Scanning Electron

Abstract The aim of the present study was to describe the morphology of the eggs of Culex (Culex) saltanensis Dyar that occurs in the Neotropical region. Eggs of the Cx. (Cux.) saltanensis were collected at the Mata Atlântica FIOCRUZ campus, fixed in 1% osmium tetroxide, prepared for mounting on metal supports, observed under a scanning electron microscope, and described morphologically. The eggs had a coniform shape with a length of approximately 0.5 mm (505–510 µm) and a width in the median portion of 117 µm (113–123 µm). Upper portion is lined with tubers of irregular shape and varying sizes (0.64–1.31 µm), located on a cross-linked matrix forming bands observed under optical microscopy. The micropyle is encased in a necklace of approximately 6.6-µm plates arranged in a flower-like shape. Comparing Cx. (Cux.) saltanensis eggs with several species of different genera, important divergent characteristics can be observed. However, this study points to the need for new descriptions of eggs of species belonging to the same subgenus in order to analyze if there will be differences between them. Culex (Cux.) saltanensis eggs have particular characteristics not observed in eggs of other Culicidae genera.

Ascospore sculpturing and generic concepts in the Testudinaceae (syn. Zopfiaceae)

1979 ◽  
Vol 57 (2) ◽  
pp. 91-99 ◽  
Author(s):  
D. L. Hawksworth
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Combination ◽  
The Family ◽  
First Time ◽  
Scanning Electron

Generic concepts in the Testudinaceae (Ascomycotina, Loculoascomycetes) are reviewed with particular emphasis on the sculpturing of the ascospores which has been examined by scanning electron microscopy (SEM) for the first time. A key to the nine genera accepted in the family is provided of which two are described as new: Ulospora for U. bilgramii comb.nov. (= Zopfia bilgramii D. Hawksw. et al.), the ascospores of which have three to six deep fissures in each cell, and Zopfiofoveola for Z. punctata comb.nov. (= Zopfia punctata D. Hawksw. & C. Booth), with distinctly and regularly foveolate ascospores. One further new combination is made: Rechingeriella boudieri comb.nov. (= Zopfia boudieri Arnaud). The positions of three additional genera referred to the family by earlier authors but which are excluded from it here are also briefly discussed.

New perspectives on Nephridiacanthus major (Acanthocephala: Oligacanthorhynchidae) collected from hedgehogs in Iran

2020 ◽  
Vol 94 ◽  
Author(s):  
O.M. Amin ◽  
M. Sharifdini ◽  
R.A. Heckmann ◽  
M. Zarean
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Analysis ◽  
Chemical Analysis ◽  
Central Asia ◽  
Molecular Profile ◽  
X Ray ◽  
The Family ◽  
First Time ◽  
Scanning Electron

We describe morphological features not previously reported for this old acanthocephalan Nephridiacanthus major (Bremser, 1811 in Westrumb, 1821) Golvan, 1962 first described over 200 years ago. Our specimens were collected from long-eared hedgehog Hemiechinus auritus (Gmelin, 1770) (Erinaceidae) in Iran. We compare the morphometrics of our material with others previously reported from the same host in Iran, Russia, central Asia and Europe. Our specimens had markedly smaller proboscides, proboscis hooks and lemnisci than those reported from Russia and central Asia, but comparable measurements of other structures with specimens previously described from other collections. We document our new observations with scanning electron microscopy features not previously demonstrable by other observers and provide a chemical analysis of proboscis hooks using energy-dispersive X-ray analysis for the first time. The molecular profile of this acanthocephalan, based on 18S rDNA and cox1 genes, was generated for the first time. The phylogenetic analysis showed that N. major is placed in a clade of the family Oligacanthorhynchidae, well separated from the families Moniliformidae and Gigantorhynchidae.

Antennal Sensilla of Chrysis shanghalensis (Hymenoptera: Chrysididae), a Larval Ectoparasite of Monema flavescens (Lepidoptera: Limacodidae)

2021 ◽  
Vol 56 (1) ◽  
pp. 1-11
Author(s):  
Shu Z. Yang ◽  
Mei H. Yang ◽  
Yun Xu ◽  
Jin T. Zhang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Enemy ◽  
Tree Species ◽  
External Morphology ◽  
Antennal Sensilla ◽  
Sensilla Basiconica ◽  
Sensilla Trichodea ◽  
Males And Females ◽  
Scanning Electron

Abstract Chrysis shanghalensis Smith (Hymenoptera: Chrysididae) is an ectoparasitoid and important natural enemy of Monema flavescens Walker (Lepidoptera: Limacodidae), a serious defoliator of a number of tree species. The external morphology of the antennal sensilla of this parasitoid was examined by scanning electron microscopy, and types and distribution of sensilla were recorded. The antennae of C. shanghalensis were geniculate in shape and composed of a scape with radicula, a pedicel, and a flagellum divided into 11 flagellomeres in males and females. Cuticular pore and 14 types of sensilla were identified on the antennae of both sexes. These included aporous Böhm's bristles, sensilla trichodea 1, 2, and 3 (nonporous), sensilla trichodea 4 (multiporous), two types of sensilla chaetica (nonporous), four morphological types of sensilla basiconica (multiporous), two morphological types of sensilla coeloconica (multiporous), and one type of sensillum campaniforme (nonporous).

scholarly journals Fine Structure of Maxillary Palps in Adults of Hermetia illucens (Diptera: Stratiomyidae)

2020 ◽  
Author(s):  
M Pezzi ◽  
C Scapoli ◽  
M Bharti ◽  
M J Faucheux ◽  
M Chicca ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sexual Dimorphism ◽  
Hermetia Illucens ◽  
The Family ◽  
Sensory Structures ◽  
First Time ◽  
Maxillary Palps ◽  
Scanning Electron

Abstract A relevant species in waste management but also in forensic, medical, and veterinary sciences is the black soldier fly, Hermetia illucens (Linnaeus; Diptera: Stratiomyidae). An ultrastructural study by scanning electron microscopy (SEM) was conducted for the first time on maxillary palps of both sexes, describing in detail the morphology and distribution of sensilla and microtrichia. The maxillary palps, composed of two segments, show sexual dimorphism in length and shape. In both sexes, the first segment is covered only by microtrichia, but the second one is divided into two parts: the proximal one, covered only by microtrichia, and the distal one containing both microtrichia and sensory structures. These structures include two types of sensory pits and one of chaetic sensilla. Due to sexual dimorphism in palp size, females have a higher number of sensory pits. The sexual dimorphism of palps and the presence and role of sensilla in H. illucens was discussed in comparison to other species of the family Stratiomyidae and of other Diptera. This study may represent a base for further investigations on mouthpart structures of this species, involved in key physiological activities, such as feeding, mating and oviposition.

Anatomical characterisation of the foliar colleters in Myrtoideae (Myrtaceae)

2012 ◽  
Vol 60 (8) ◽  
pp. 707 ◽  
Author(s):  
Cleber J. da Silva ◽  
Luiz Cláudio de A. Barbosa ◽  
Ana E. Marques ◽  
Maria Cristina Baracat-Pereira ◽  
Antônio L. Pinheiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Relationships ◽  
External Morphology ◽  
Secretory Structures ◽  
Reproductive Shoot ◽  
Sds Page ◽  
Histochemical Tests ◽  
Five Tribes ◽  
Scanning Electron

Colleters are secretory structures that occur in vegetative or reproductive shoot apices of many botanical families. However, in the order Myrtales, reports of colleters have considered only external morphology. We therefore evaluated apical meristems of 52 species belonging to 17 genera from seven tribes of subfamily Myrtoideae (Myrtaceae), so as to analyse the incidence and morphological types of colleters. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on fresh and methacrylate-embedded material. Proteins of the colleter secretions were analysed by SDS-PAGE. We have classified and described the following three new colleter types: petaloid, conic and euryform. None of the species contained all three colleter types. The petaloid colleters were present in three tribes (Syzygieae, Melaleuceae and Lophostemoneae). The conic colleters were observed in three tribes (Leptospermeae, Myrteae and Melaleuceae) and the euryform type occurred in five tribes (Leptospermeae, Syncarpieae, Myrteae, Syzygieae and Melaleuceae). In the tribe Eucalypteae, we found no evidence of colleters. The presence of mucilaginous secretion that defines colleters was confirmed by histochemical tests, and no proteins were found in the secretion. The colleters in Myrtoideae may help clarify the phylogenetic relationships of the Myrtaceae family.

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