Rotational Doppler shift of the light transmitted behind a rotating object with rotational symmetries: rotational Doppler shift of the transmitted light

2022 ◽  
Vol 76 (1) ◽  
Author(s):  
Olivier Emile ◽  
Janine Emile ◽  
Christian Brousseau ◽  
Tangi le Guennic ◽  
Pu Jian ◽  
...  
Keyword(s):  
Doppler Shift ◽  
Transmitted Light

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Three dimensional deblurring of transmitted-light brightfield micrographs

1993 ◽  
Vol 51 ◽  
pp. 564-565
Author(s):  
Santosh Bhattacharyya
Keyword(s):  
Image Reconstruction ◽  
Three Dimensional ◽  
Transmitted Light ◽  
Reconstruction Algorithms ◽  
3D Image Reconstruction ◽  
Structural Details ◽  
Spread Function ◽  
Early Testing ◽  
Linearized Model ◽  
Image Reconstruction Algorithms

Three dimensional microscopic structures play an important role in the understanding of various biological and physiological phenomena. Structural details of neurons, such as the density, caliber and volumes of dendrites, are important in understanding physiological and pathological functioning of nervous systems. Even so, many of the widely used stains in biology and neurophysiology are absorbing stains, such as horseradish peroxidase (HRP), and yet most of the iterative, constrained 3D optical image reconstruction research has concentrated on fluorescence microscopy. It is clear that iterative, constrained 3D image reconstruction methodologies are needed for transmitted light brightfield (TLB) imaging as well. One of the difficulties in doing so, in the past, has been in determining the point spread function of the system.We have been developing several variations of iterative, constrained image reconstruction algorithms for TLB imaging. Some of our early testing with one of them was reported previously. These algorithms are based on a linearized model of TLB imaging.

Doppler shift effect on infrared band models

1989 ◽  
Author(s):  
J. DRAKES ◽  
R. HIERS ◽  
R. REED
Keyword(s):  
Doppler Shift ◽  
Infrared Band ◽  
Shift Effect

Portal Blood Flow as Measured by the Doppler Shift Technique in Sheep Fed Chopped Forages

1975 ◽  
Vol 41 (2) ◽  
pp. 596-600 ◽  
Author(s):  
L. R. Prewitt ◽  
D. R. Jacobson ◽  
R. W. Hemken ◽  
R. H. Hatton
Keyword(s):  
Blood Flow ◽  
Doppler Shift ◽  
Portal Blood Flow ◽  
Portal Blood ◽  
Shift Technique

Branching Ratios and Lifetimes of 25Mg Levels below 5.2 MeV

1974 ◽  
Vol 52 (23) ◽  
pp. 2329-2342 ◽  
Author(s):  
R. W. Ollerhead ◽  
D. C. Kean ◽  
R. M. Gorman ◽  
M. B. Thomson
Keyword(s):  
Doppler Shift ◽  
Gamma Ray ◽  
Branching Ratios ◽  
Surface Barrier ◽  
Surface Barrier Detector ◽  
Rotational Model ◽  
Rotational Bands ◽  
Barrier Detector ◽  
Combined Data

All levels below 5.2 MeV in 25Mg have been studied using the reaction 25Mg(p, p′γ). In-elastically scattered protons were detected in an annular surface barrier detector located at 180°; coincidence gamma-ray spectra were obtained at Ge (Li) detector angles of 90°, 45°, and 135°. Level energies were determined from unshifted gamma-ray energies recorded in the 90° spectra. Lifetimes were obtained from the attenuated Doppler shift of gamma-ray energies recorded in spectra taken at forward and backward angles. Branching ratios were deduced from the combined data of all three angles. The identification of levels as members of rotational bands is discussed, and transition strengths deduced from the present measurements are compared with predictions of the simple rotational model.

scholarly journals The Ad Hoc Back-End of the BIRALET Radar to Measure Slant-Range and Doppler Shift of Resident Space Objects

Electronics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 577
Author(s):  
Luca Schirru ◽  
Tonino Pisanu ◽  
Angelo Podda
Keyword(s):  
Doppler Shift ◽  
Space Debris ◽  
Ad Hoc ◽  
Low Earth Orbit ◽  
Slant Range ◽  
Radar Systems ◽  
The Earth ◽  
Space Objects ◽  
Resident Space Objects ◽  
Potential Issue

Space debris is a term for all human-made objects orbiting the Earth or reentering the atmosphere. The population of space debris is continuously growing and it represents a potential issue for active satellites and spacecraft. New collisions and fragmentation could exponentially increase the amount of debris and so the level of risk represented by these objects. The principal technique used for the debris monitoring, in the Low Earth Orbit (LEO) between 200 km and 2000 km of altitude, is based on radar systems. The BIRALET system represents one of the main Italian radars involved in resident space objects observations. It is a bi-static radar, which operates in the P-band at 410–415 MHz, that uses the Sardinia Radio Telescope as receiver. In this paper, a detailed description of the new ad hoc back-end developed for the BIRALET radar, with the aim to perform slant-range and Doppler shift measurements, is presented. The new system was successfully tested in several validation measurement campaigns, the results of which are reported and discussed.

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