Locally Administered Nerve Growth Factor Suppresses Ginsenoside  Rb1-enhanced Peripheral Nerve Regeneration

A high-dose of nerve growth factor (NGF) mixed with ginsenoside Rb 1( GRb 1) was encapsulated by collagen and placed in silicone rubber chambers, which were used to repair dissected Sprague-Dawley rat sciatic nerves with 15 mm gaps. Six weeks after surgery, no axons or Schwann cells were seen in these chambers. By comparison, nerves treated with collagen- GRb 1 alone had regenerated axons and Schwann cells in their endoneurial areas. We suggest that excessive NGF may not promote but, rather, suppress developing nerves.

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The effects of gradients of nerve growth factor immobilized PCLA scaffolds on neurite outgrowth in vitro and peripheral nerve regeneration in rats

Biomaterials ◽

10.1016/j.biomaterials.2013.05.080 ◽

2013 ◽

Vol 34 (29) ◽

pp. 7086-7096 ◽

Cited By ~ 96

Author(s):

Shuo Tang ◽

Jixiang Zhu ◽

Yangbin Xu ◽

Andy Peng Xiang ◽

Mei Hua Jiang ◽

...

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Peripheral Nerve ◽

Neurite Outgrowth ◽

Nerve Regeneration ◽

Peripheral Nerve Regeneration ◽

Nerve Growth

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Purification of Schwann cells by selection of p75 low affinity nerve growth factor receptor expressing cells from adult peripheral nerve

Journal of Neuroscience Methods ◽

10.1016/s0165-0270(02)00382-5 ◽

2003 ◽

Vol 124 (2) ◽

pp. 135-143 ◽

Cited By ~ 62

Author(s):

Maurice Vroemen ◽

Norbert Weidner

Keyword(s):

Nerve Growth Factor ◽

Growth Factor ◽

Peripheral Nerve ◽

Schwann Cells ◽

Growth Factor Receptor ◽

Nerve Growth Factor Receptor ◽

Nerve Growth ◽

Selection Of

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Preparation and characterization of injectable chitosan–hyaluronic acid hydrogels for nerve growth factor sustained release

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911516662068 ◽

2016 ◽

Vol 32 (2) ◽

pp. 146-162 ◽

Cited By ~ 13

Author(s):

Haixing Xu ◽

Lingxi Zhang ◽

Yun Bao ◽

Xiumei Yan ◽

Yixia Yin ◽

...

Keyword(s):

Hyaluronic Acid ◽

Nerve Growth Factor ◽

Growth Factor ◽

Peripheral Nerve ◽

Sustained Release ◽

Peripheral Nerve Regeneration ◽

Nerve Growth ◽

Nerve Conduits ◽

Hyaluronic Acid Hydrogel

The usage of hollow nerve conduits shows inferior recovery effect on the repair of peripheral nerve defects. In this study, a biocompatible and biodegradable pH-induced injectable chitosan–hyaluronic acid hydrogel for nerve growth factor encapsulation and sustained release was developed as the fillers in the lumen of hollow nerve conduit to reform its microenvironment for peripheral nerve regeneration. The physicochemical properties of hydrogel were characterized by gelation time, Fourier transform infrared spectroscopy, scanning electron microscopy, compressive modulus, porosity, swelling ratio, and in vitro degradation. The in vitro nerve growth factor release profiles and cell evaluation were also investigated. The results show that the structure of chitosan–hyaluronic acid hydrogel is composed of interconnected channels with a controllable pore diameter ranging from 20 to 100 µm. The hydrogel can be degraded more than 70% within 8 weeks in vitro and is available for nerve growth factor sustained release. The chitosan–hyaluronic acid/nerve growth factor hydrogel is non-toxic and suitable for adhesion and proliferation of nerve cells and capable of maintaining nerve growth factor activity. Therefore, it could be a promising intraluminal filler of nerve conduits for peripheral nerve regeneration in neural tissue engineering.

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