LOW-LYING $\frac52^{+}$ RESONANCE IN 11Be: BOUND STATE IN THE CONTINUUM

2009 ◽  
Vol 18 (08) ◽  
pp. 1741-1749 ◽  
Author(s):  
SABYASACHI MAHAPATRA ◽  
TAPAN KUMAR DAS ◽  
SAGAR KUMAR DUTTA

We have used a novel theoretical technique to calculate the [Formula: see text] resonance state of 11 Be using a two-body model (10 Be+n ). The effective two-body potential for the system has a shallow well followed by a low and very wide barrier causing numerical difficulties in the calculation of low-lying resonances. Using supersymmetric quantum mechanics (SSQM), one can construct an isospectral potential with a bound state in the continuum (BIC). This isospectral potential has a deep well and high barrier which can effectively trap the system giving rise to a BIC. Our calculated resonance energy of the [Formula: see text] state of 11 Be matches extremely well with the experimental value. Calculated width of the resonance also agrees within the computational error bars.

Author(s):  
Ondrej Gutten ◽  
Petr Jurečka ◽  
Zahra Aliakbar Tehrani ◽  
Miloš Buděšínský ◽  
Jan Řezáč ◽  
...  

Computational “error bars” for modelling cyclic dinucleotides – NMR experiment vs. quantum mechanics and molecular dynamics.


2018 ◽  
Vol 1092 ◽  
pp. 012012 ◽  
Author(s):  
M. Balyzin ◽  
Z. Sadrieva ◽  
M. Belyakov ◽  
P. Kapitanova ◽  
A. Sadreev ◽  
...  

Antibodies ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 8 ◽  
Author(s):  
Laura Keller ◽  
Nicolas Bery ◽  
Claudine Tardy ◽  
Laetitia Ligat ◽  
Gilles Favre ◽  
...  

RHO (Ras HOmologous) GTPases are molecular switches that activate, in their state bound to Guanosine triphosphate (GTP), key signaling pathways, which involve actin cytoskeleton dynamics. Previously, we selected the nanobody RH12, from a synthetic phage display library, which binds the GTP-bound active conformation of RHOA (Ras Homologous family member A). However, when expressed as an intracellular antibody, its blocking effect on RHO signaling led to a loss of actin fibers, which in turn affected cell shape and cell survival. Here, in order to engineer an intracellular biosensor of RHOA-GTP activation, we screened the same phage nanobody library and identified another RHO-GTP selective intracellular nanobody, but with no apparent toxicity. The recombinant RH57 nanobody displays high affinity towards GTP-bound RHOA/B/C subgroup of small GTPases in vitro. Intracellular expression of the RH57 allowed selective co-precipitation with the GTP-bound state of the endogenous RHOA subfamily. When expressed as a fluorescent fusion protein, the chromobody GFP-RH57 was localized to the inner plasma membrane upon stimulation of the activation of endogenous RHO. Finally, the RH57 nanobody was used to establish a BRET-based biosensor (Bioluminescence Resonance Energy Transfer) of RHO activation. The dynamic range of the BRET signal could potentially offer new opportunities to develop cell-based screening of RHOA subfamily activation modulators.


2020 ◽  
Author(s):  
F. A. Benimetskiy ◽  
V. Kravtsov ◽  
E. Khestanova ◽  
I. Sinev ◽  
A. Samusev ◽  
...  

2009 ◽  
Vol 24 (11) ◽  
pp. 2191-2197 ◽  
Author(s):  
T. MATSUMOTO ◽  
T. EGAMI ◽  
K. OGATA ◽  
Y. ISERI ◽  
M. KAMIMURA ◽  
...  

We present analyses of breakup effects of 6 He on the elastic scattering by the continuum-discretized coupled-channels method, in which the reaction system is described as a four-body model, n+n+4 He +target. In this analysis, three-body breakup continuum of 6 He is discretized by daiagonalizing the internal Hamiltonian of 6 He in a space spanned by the Gaussian basis functions. The calculated elastic cross sections are in good agreement with the experimental data, which shows that nuclear and Coulomb breakup effects are significant.


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