Terminal Bulb Segmentation of Caenorhabditis Elegans under Small Samples Based on Two-stage U-Net Network

Author(s):  
Si Cao ◽  
Jun Liu
1977 ◽  
Author(s):  
Mark Weinstein ◽  
Daniel Deykin

A novel two-stage SDS gel electrophoretic procedure was devised to examine the molecular weight heterogeneity of fibrinogen in small samples of whole plasma from 12 normal and 7 cirrhotic individuals. Fibrinogen was first separated from other plasma proteins on a large pore gel, cut out of the gel, reduced, and separated into its component Aα, Bβ, and γ chains on a second gel. Two major mol wt species—fibrinogen I and II—were observed on the first gel. The ~ 25000 mol wt difference between these two forms reflected a decrease primarily in the size of one of the fibrinogen II Aα chains. In both normals and cirrhotic patients fibrinogen II comprised 30% of the total (range 20–35%). Fibrinogen I and II each contained two major high mol wt Aα chains—Aα/1 and a smaller Aα/2—that differ by 3000 mol wt. In normal fibrinogen I, Aα/2 comprised 33% of the total Aα chains (range 27–41%). In contrast the fibrinogen I of 6 out of the 7 patients had a lower per cent of Aα/2 (range 10–25%). Similar quantitative differences were seen in the decreased fraction of Aα/2 in the fibrinogen II of cirrhotic patients compared to normals. No correlation was found between per cent fibrinogen II and per cent Aα/2 in either normal subjects or cirrhotics. These results suggest that at least two independent processes are responsible for the observed levels of Aα chain heterogeneity in normals and cirrhotics and that one of these processes yields a lower than normal fraction of Aa/2 chains in the fibrinogen of cirrhotic individuals.


Author(s):  
Sengshiu Chung ◽  
Peggy Cebe

We are studying the crystallization and annealing behavior of high performance polymers, like poly(p-pheny1ene sulfide) PPS, and poly-(etheretherketone), PEEK. Our purpose is to determine whether PPS, which is similar in many ways to PEEK, undergoes reorganization during annealing. In an effort to address the issue of reorganization, we are studying solution grown single crystals of PPS as model materials.Observation of solution grown PPS crystals has been reported. Even from dilute solution, embrionic spherulites and aggregates were formed. We observe that these morphologies result when solutions containing uncrystallized polymer are cooled. To obtain samples of uniform single crystals, we have used two-stage self seeding and solution replacement techniques.


2002 ◽  
Vol 69 ◽  
pp. 117-134 ◽  
Author(s):  
Stuart M. Haslam ◽  
David Gems ◽  
Howard R. Morris ◽  
Anne Dell

There is no doubt that the immense amount of information that is being generated by the initial sequencing and secondary interrogation of various genomes will change the face of glycobiological research. However, a major area of concern is that detailed structural knowledge of the ultimate products of genes that are identified as being involved in glycoconjugate biosynthesis is still limited. This is illustrated clearly by the nematode worm Caenorhabditis elegans, which was the first multicellular organism to have its entire genome sequenced. To date, only limited structural data on the glycosylated molecules of this organism have been reported. Our laboratory is addressing this problem by performing detailed MS structural characterization of the N-linked glycans of C. elegans; high-mannose structures dominate, with only minor amounts of complex-type structures. Novel, highly fucosylated truncated structures are also present which are difucosylated on the proximal N-acetylglucosamine of the chitobiose core as well as containing unusual Fucα1–2Gal1–2Man as peripheral structures. The implications of these results in terms of the identification of ligands for genomically predicted lectins and potential glycosyltransferases are discussed in this chapter. Current knowledge on the glycomes of other model organisms such as Dictyostelium discoideum, Saccharomyces cerevisiae and Drosophila melanogaster is also discussed briefly.


2007 ◽  
Vol 177 (4S) ◽  
pp. 121-121
Author(s):  
Antonio Dessanti ◽  
Diego Falchetti ◽  
Marco Iannuccelli ◽  
Susanna Milianti ◽  
Gian P. Strusi ◽  
...  
Keyword(s):  

2007 ◽  
Vol 177 (4S) ◽  
pp. 120-120
Author(s):  
Pamela I. Ellsworth ◽  
Anthony Caldamone
Keyword(s):  

2005 ◽  
Vol 38 (18) ◽  
pp. 68
Author(s):  
SHARON WORCESTER
Keyword(s):  

Crisis ◽  
2013 ◽  
Vol 34 (6) ◽  
pp. 434-437 ◽  
Author(s):  
Donald W. MacKenzie

Background: Suicide clusters at Cornell University and the Massachusetts Institute of Technology (MIT) prompted popular and expert speculation of suicide contagion. However, some clustering is to be expected in any random process. Aim: This work tested whether suicide clusters at these two universities differed significantly from those expected under a homogeneous Poisson process, in which suicides occur randomly and independently of one another. Method: Suicide dates were collected for MIT and Cornell for 1990–2012. The Anderson-Darling statistic was used to test the goodness-of-fit of the intervals between suicides to distribution expected under the Poisson process. Results: Suicides at MIT were consistent with the homogeneous Poisson process, while those at Cornell showed clustering inconsistent with such a process (p = .05). Conclusions: The Anderson-Darling test provides a statistically powerful means to identify suicide clustering in small samples. Practitioners can use this method to test for clustering in relevant communities. The difference in clustering behavior between the two institutions suggests that more institutions should be studied to determine the prevalence of suicide clustering in universities and its causes.


Sign in / Sign up

Export Citation Format

Share Document