Analysis of cloned cDNAs differentially expressed in adapting remnant small intestine after partial resection

1996 ◽  
Vol 271 (2) ◽  
pp. G347-G356 ◽  
Author(s):  
B. D. Dodson ◽  
J. L. Wang ◽  
E. A. Swietlicki ◽  
D. C. Rubin ◽  
M. S. Levin
Keyword(s):  
Small Intestine ◽  
Adaptive Response ◽  
Binding Protein ◽  
Intestinal Adaptation ◽  
Intestinal Resection ◽  
Partial Resection ◽  
Differentially Expressed ◽  
Cdna Clones ◽  
Regional Patterns ◽  
Fatty Acid Binding

After partial resection, the remnant small intestine undergoes an adaptive response. Little is known about the molecular and cellular basis of intestinal adaptation. To identify genes transcriptionally regulated in response to loss of functional bowel surface area, we have isolated cDNAs differentially expressed in the adaptive ileum 48 h after 70% proximal small intestinal resection. A cDNA library constructed from the remnant ileum of rats subjected to resection was screened using subtractive hybridization techniques. Several groups of cDNA clones that were induced during intestinal adaptation were isolated. The first included liver fatty acid binding protein, apolipoprotein A-IV, cellular retinol binding protein II, and ileal lipid binding protein. These all encode proteins involved in the absorption, metabolism, and trafficking of nutrients. A second group included the catalytic subunit of protein phosphatase 1 delta, a 78-kDa glucose-regulated protein (grp 78; a glucose-regulated member of the 70-kDa heat-shock protein family), and several pancreatitis-associated proteins. A third group of induced genes contained novel cDNAs. To better characterize the adaptive response, the temporal, spatial, and cellular patterns of expression of several of these genes were analyzed with the use of immunohistochemical and in situ hybridization techniques. These studies indicate that during early adaptation, genes involved in nutrient trafficking, protein processing, and cell cycle regulation are transcriptionally regulated in the residual small intestine in distinct temporal and regional patterns consistent with a complex multifaceted response to intestinal resection.

New insights into the fatty acid-binding protein (FABP) family in the small intestine

2002 ◽  
pp. 139-147 ◽  
Author(s):  
Philippe Besnard ◽  
Isabelle Niot ◽  
Hélène Poirier ◽  
Lionel Clément ◽  
André Bernard
Keyword(s):  
Small Intestine ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Perinatal ontogeny of fatty acid binding protein activity in porcine small intestine

1992 ◽  
Vol 12 (11) ◽  
pp. 1345-1356 ◽  
Author(s):  
Gregory A. Reinhart ◽  
Frank A. Simmen ◽  
Donald C. Mahan ◽  
Rosalia C.M. Simmen ◽  
Michael E. White ◽  
...  
Keyword(s):  
Small Intestine ◽  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Protein Activity ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

scholarly journals Differential expression of fatty acid binding protein 4 in cancers of the breast.

2021 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Breast Cancer ◽  
Fatty Acid ◽  
Differential Expression ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Normal Breast ◽  
Differentially Expressed ◽  
Primary Tumors ◽  
Fatty Acid Binding ◽  
Acid Binding Protein

Breast cancer affects women at relatively high frequency (1). We mined published microarray datasets (2, 3) to determine in an unbiased fashion and at the systems level genes most differentially expressed in the primary tumors of patients with breast cancer. We report here significant differential expression of the gene encoding fatty acid binding protein 4, FABP4, when comparing primary tumors of the breast to the tissue of origin, the normal breast. FABP4 mRNA was present at significantly lower quantities in tumors of the breast as compared to normal breast tissue. Analysis of human survival data revealed that expression of FABP4 in primary tumors of the breast was correlated with recurrence-free survival in patients with HER2+ cancers, demonstrating a relationship between primary tumor expression of a differentially expressed gene and patient survival outcomes influenced by molecular subtype. FABP4 may be of relevance to initiation, maintenance or progression of cancers of the female breast.

scholarly journals The human milk oligosaccharide 2′-fucosyllactose augments the adaptive response to extensive intestinal

2016 ◽  
Vol 310 (6) ◽  
pp. G427-G438 ◽  
Author(s):  
Ethan A. Mezoff ◽  
Jennifer A. Hawkins ◽  
Nicholas J. Ollberding ◽  
Rebekah Karns ◽  
Ardythe L. Morrow ◽  
...  
Keyword(s):  
Weight Gain ◽  
Human Milk ◽  
Adaptive Response ◽  
Intestinal Adaptation ◽  
Intestinal Resection ◽  
Cost Of Care ◽  
Transcriptional Analysis ◽  
Ileocecal Resection ◽  
Milk Oligosaccharide ◽  
Human Milk Oligosaccharide

Intestinal resection resulting in short bowel syndrome (SBS) carries a heavy burden of long-term morbidity, mortality, and cost of care, which can be attenuated with strategies that improve intestinal adaptation. SBS infants fed human milk, compared with formula, have more rapid intestinal adaptation. We tested the hypothesis that the major noncaloric human milk oligosaccharide 2′-fucosyllactose (2′-FL) contributes to the adaptive response after intestinal resection. Using a previously described murine model of intestinal adaptation, we demonstrated increased weight gain from 21 to 56 days ( P < 0.001) and crypt depth at 56 days ( P < 0.0095) with 2′-FL supplementation after ileocecal resection. Furthermore, 2′-FL increased small bowel luminal content microbial alpha diversity following resection ( P < 0.005) and stimulated a bloom in organisms of the genus Parabacteroides (log2-fold = 4.1, P = 0.035). Finally, transcriptional analysis of the intestine revealed enriched ontologies and pathways related to antimicrobial peptides, metabolism, and energy processing. We conclude that 2′-FL supplementation following ileocecal resection increases weight gain, energy availability through microbial community modulation, and histological changes consistent with improved adaptation.

Expression of a liver fatty acid binding protein/human decay-accelerating factor/HLA-B44 chimeric gene in transgenic mice

1991 ◽  
Vol 260 (6) ◽  
pp. G929-G939
Author(s):  
J. R. Hansbrough ◽  
D. M. Lublin ◽  
K. A. Roth ◽  
E. A. Birkenmeier ◽  
J. I. Gordon
Keyword(s):  
Fatty Acid ◽  
Transgenic Mice ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Liver Fatty Acid ◽  
Regional Patterns ◽  
Decay Accelerating Factor ◽  
Fatty Acid Binding ◽  
Cis Acting ◽  
Acid Binding Protein

The intestinal epithelium is characterized by the rapid and continuous renewal of its four principal cell types and by its ability to establish and maintain remarkably complex spatial differentiation along its crypt-to-villus and duodenal-to-colonic axes. We have previously used transgenic mice containing liver fatty acid binding protein/human growth hormone (L-FABP/hGH) fusion genes to analyze the molecular mechanisms responsible for encoding positional information in this epithelium. Because these studies could not distinguish whether cis-acting sequences in the L-FABP promoter or hGH structural gene were responsible for the observed cellular and regional patterns of transgene transcription in the gut, a second model fusion gene has now been constructed. It consists of nucleotides -596 to +21 of rat L-FABP linked to a cDNA encoding a chimeric protein, human decay-accelerating factor (DAF, minus the site of attachment of its COOH-terminal glycophospholipid anchor), coupled to the transmembrane (TM) and cytoplasmic domains of human HLA-B44. RNA blot hybridization and immunocytochemical analyses revealed that the cell-specific and region-specific expressions of DAF-TM and hGH in adult mice appear identical along both axes of the gut, indicating that cis-acting elements contained within the 5' nontranscribed region of the L-FABP gene rather than in the reporter are largely responsible for these observed patterns of transgene expression. Unlike pre-hGH, a prototypical secreted protein, DAF-TM is a membrane protein. The ability to direct its expression along the length of both axes of the gut provides an opportunity to analyze in vivo the sorting pathways of membrane-associated proteins in normal epithelial cells as a function of their location and differentiation. Light microscopic studies indicate that DAF-TM is targeted to the basolateral and apical surfaces of villus-associated enterocytes.

Enterocytic gene expression in intestinal adaptation: evidence for a specific cellular response

1996 ◽  
Vol 270 (1) ◽  
pp. G143-G152 ◽  
Author(s):  
D. C. Rubin ◽  
E. A. Swietlicki ◽  
J. L. Wang ◽  
B. D. Dodson ◽  
M. S. Levin
Keyword(s):  
Small Bowel ◽  
Adaptive Response ◽  
Cellular Response ◽  
Bowel Resection ◽  
Intestinal Adaptation ◽  
Small Bowel Resection ◽  
Mrna Levels ◽  
Fatty Acid Binding ◽  
Mrna And Protein Expression ◽  
Differentiation Status

After massive small bowel resection, the remnant gut epithelium undergoes an adaptive response marked by an increase in villus height, crypt depth, and crypt cell production rate. Although morphological features of gut adaptation have been well characterized, the differentiation status and response of epithelial cells populating the adaptive villus is unclear. To address these issues, cell-specific and spatial patterns of expression of a set of enterocytic genes were characterized in rats after 70% small bowel resection. The liver and intestinal (I) fatty acid binding protein (FABP) and apolipoprotein A-I (apo A-I) and apo A-IV genes were studied because they exhibit unique regional and cell-specific patterns of expression in the developing and adult gut. At 48 h after surgery, apo A-IV and I-FABP mRNA levels were increased up to 3.5-fold in adaptive remnant ileum compared with sham-operated or sham-resected control ileum. In situ hybridization and immunohistochemical analyses revealed a marked increase in enterocytic apo A-IV mRNA and protein expression in the adaptive ileum, from villus base to tip but not in crypts. By 1 wk after resection, apo A-IV, but not I-FABP, mRNA levels remained elevated in remnant ileum, although duodenal I-FABP mRNA levels were still increased. In contrast, apo A-I mRNA levels were not significantly induced. These results indicate that the enterocyte can respond acutely to loss of small bowel surface area by increasing expression of several genes. This compensatory enterocytic response is spatially (from duodenum to ileum) and temporally regulated. These results suggest initiation of the adaptive response occurs by way of a complex set of molecular pathways involving villus and crypt cells.

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