Comparison of buffer and red blood cell perfusion of guinea pig heart oxygenation

2003 ◽  
Vol 285 (5) ◽  
pp. H1819-H1825 ◽  
Author(s):  
Kenneth A. Schenkman ◽  
Daniel A. Beard ◽  
Wayne A. Ciesielski ◽  
Eric O. Feigl
Keyword(s):  
Oxygen Consumption ◽  
Guinea Pig ◽  
Oxygen Saturation ◽  
Red Blood Cells ◽  
Ventricular Function ◽  
Oxygen Tension ◽  
Blood Cells ◽  
Low Oxygen ◽  
Guinea Pig Heart ◽  
Perfused Hearts

Myocardial mean myoglobin oxygen saturation was determined spectroscopically from isolated guinea pig hearts perfused with red blood cells during increasing hypoxia. These experiments were undertaken to compare intracellular myoglobin oxygen saturation in isolated hearts perfused with a modest concentration of red blood cells (5% hematocrit) with intracellular myoglobin saturation previously reported from traditional buffer-perfused hearts. Studies were performed at 37°C with hearts paced at 240 beats/min and a constant perfusion pressure of 80 cmH2O. It was found that during perfusion with a hematocrit of 5%, baseline mean myoglobin saturation was 93% compared with 72% during buffer perfusion. Mean myoglobin saturation, ventricular function, and oxygen consumption remained fairly constant for arterial perfusate oxygen tensions above 100 mmHg and then decreased precipitously below 100 mmHg. In contrast, mean myoglobin saturation, ventricular function, and oxygen consumption began to decrease even at high oxygen tension with buffer perfusion. The present results demonstrate that perfusion with 5% red blood cells in the perfusate increases the baseline mean myoglobin saturation and better preserves cardiac function at low oxygen tension relative to buffer perfusion. These results suggest that caution should be used in extrapolating intracellular oxygen dynamics from buffer-perfused to blood-perfused hearts.

scholarly journals Inhibition of Rho Kinase by Y27632 Increases Low Oxygen Tension‐Induced ATP Release from Human Red Blood Cells (RBCs)

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Kelly Thuet ◽  
Elizabeth Bowles ◽  
Meera Sridharan ◽  
Shaquria Adderley ◽  
Randy Sprague ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Oxygen Tension ◽  
Blood Cells ◽  
Atp Release ◽  
Rho Kinase ◽  
Low Oxygen ◽  
Human Red Blood Cells ◽  
Low Oxygen Tension

Oxygen consumption in the avascular guinea pig retina

1996 ◽  
Vol 271 (3) ◽  
pp. H1162-H1165 ◽  
Author(s):  
S. Cringle ◽  
D. Y. Yu ◽  
V. Alder ◽  
E. N. Su ◽  
P. Yu
Keyword(s):  
Oxygen Consumption ◽  
Guinea Pig ◽  
Oxygen Tension ◽  
Physiological State ◽  
High Oxygen ◽  
Excess Oxygen ◽  
Low Oxygen ◽  
Inner Retina ◽  
Oxygen Sensitive

Oxygen consumption across the retina of a mammal with a naturally avascular retina has not previously been investigated. The oxygen consumption across the avascular retina of the guinea pig was measured in vivo by spatial analysis of the intraretinal oxygen profile. The avascular nature of the guinea pig retina allows the inner retina to be included in the analysis without disrupting the normal physiological state of the retina. Oxygen-sensitive microelectrodes (1-micron tip) were used to make high-resolution measurements of oxygen tension through the retina of anesthetized, mechanically ventilated guinea pigs (n = 10). Oxygen profiles were then analyzed in terms of oxygen tension as a function of distance from the choriocapillaris/Bruch's membrane, and the data were fitted to an established mathematical model of retinal oxygen consumption. The average oxygen consumption of the full thickness of the guinea pig retina was 1.1 +/- 0.09 ml O2.min-1 x 100 g-1 (n = 10). The average oxygen consumption of the outer half of the retina was 2.07 +/- 0.17 ml O2.min-1 x 100 g-1, while that of the inner half was only 0.12 +/- 0.04 ml O2.min-1 x 100 g-1. A localized region of high oxygen consumption was identified in the outer retina in every case, and this region accounted for an average of 93.9 +/- 2.0% of the total retinal oxygen consumption. Only 5.2 +/- 1.4% of the total oxygen consumption was attributable to the inner half of the retina. When choroidal oxygen tension was increased via a combination of systemic hyperoxia and hypercapnia, high oxygen levels could be sustained in all retinal layers. Under these conditions of an excess oxygen supply, the inner retina still consumed only 0.45 +/- 0.11 ml O2.min-1 x 100 g-1, which was 13.8 +/- 2.5% of the total retinal oxygen consumed. The relatively low oxygen consumption in the inner retina of the guinea pig may reflect an interesting adaptation to the constraints imposed by the absence of a retinal circulation in this species.

scholarly journals Mycoplasma pneumoniae Pneumonia Associated With Methemoglobinemia and Anemia: An Overlooked Association?

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
Tawfik Khoury ◽  
Ayman Abu Rmeileh ◽  
Jonathan David Kornspan ◽  
Roy Abel ◽  
Meir Mizrahi ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Red Blood Cells ◽  
Mycoplasma Pneumoniae ◽  
Blood Cells ◽  
Low Oxygen ◽  
Putative Protein ◽  
Mycoplasma Pneumoniae Pneumonia

Abstract We report a case of acute methemoglobinemia and anemia in a patient with Mycoplasma pneumoniae pneumonia. We suggest that M. pneumoniae secretes a putative protein that can induce methemoglobin in red blood cells. Thus, Mycoplasma pneumoniae may induce methemoglobinemia in patients who have low oxygen saturation and anemia.

Oxygenation-activated K fluxes in trout red blood cells

1992 ◽  
Vol 263 (5) ◽  
pp. C1057-C1064 ◽  
Author(s):  
O. B. Nielsen ◽  
G. Lykkeboe ◽  
A. R. Cossins
Keyword(s):  
Red Blood Cells ◽  
Oxygen Tension ◽  
Blood Cells ◽  
High Oxygen ◽  
Low Oxygen ◽  
Cell Shrinkage ◽  
Osmotic Swelling ◽  
Volume Regulatory Decrease ◽  
Low Oxygen Tension ◽  
A Cell

The effect of oxygenation on the dissipative fluxes of K in trout red blood cells has been determined. Unidirectional influx under low oxygen tension (PO2 = 1 kPa) was 0.56 +/- 0.07 mmol.l-1 packed cells.h-1. Within a few minutes of equilibration with high oxygen tension (PO2 = 120 kPa), influx was increased 14-fold, and this was associated with a progressive loss of KCl and a cell shrinkage. K influx progressively declined over the following 3 h to levels close to those characteristic of cells at low oxygen tension. Replacement of medium Cl by NO3- or methane sulfonate inhibited the stimulation due to high oxygen as did furosemide and low extracellular pH. The oxygenation-stimulated influx was highly volume sensitive, being increased by up to 100% by osmotic swelling and decreased by osmotic shrinkage. By contrast, the small influx under low oxygen tension was unaffected by either Cl replacement or by shrinkage and increased only with extreme swelling. Thus high oxygen tension activated a Cl-dependent and furosemide-sensitive K flux. Once activated, the mechanism was rapidly deactivated on transfer back to low oxygen tension but slowly deactivated when maintained at high PO2. The oxygenation-stimulated flux mechanism promotes a rapid and more complete volume regulatory decrease than in cells at low oxygen tension.

scholarly journals Pannexin 1 (PANX1) is a Conduit for ATP Released from Human Red Blood Cells (RBCs) Exposed to Low Oxygen Tension

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Meera Sridharan ◽  
Shaquria Adderley ◽  
Elizabeth Bowles ◽  
Alan Stephenson ◽  
Mary Ellsworth ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Oxygen Tension ◽  
Blood Cells ◽  
Low Oxygen ◽  
Pannexin 1 ◽  
Human Red Blood Cells ◽  
Low Oxygen Tension

THE UNIQUE FETAL RED CELL AND ITS FUNCTION

PEDIATRICS ◽  
1973 ◽  
Vol 51 (3) ◽  
pp. 494-500 ◽  
Author(s):  
Frank A. Oski
Keyword(s):  
Red Blood Cells ◽  
Newborn Infant ◽  
Blood Cells ◽  
Human Fetus ◽  
Exchange Transfusion ◽  
Oxygen Atmosphere ◽  
Hypoxic Stress ◽  
Red Cell ◽  
Low Oxygen ◽  
Rapid Replacement

The red blood cells of the human fetus differ in many major respects from the red cells of the normal adult. These differences appear admirably suited for the acquisition, transport, and release of oxygen in the low oxygen atmosphere of intrauterine existence. These same differences appear to confer a handicap to the cell in the extrauterine environment, particularly under conditions of hypoxic stress. The rapid replacement of these cells by artificial means, such as early exchange transfusion, may offer an advantage to the newborn infant in certain clinical situations.

Measurement of hemoglobin oxygen saturation in capillaries

1987 ◽  
Vol 252 (5) ◽  
pp. H1031-H1040 ◽  
Author(s):  
M. L. Ellsworth ◽  
R. N. Pittman ◽  
C. G. Ellis
Keyword(s):  
Light Intensity ◽  
Oxygen Saturation ◽  
Red Blood Cells ◽  
Optical Density ◽  
Blood Cells ◽  
Striated Muscle ◽  
Cheek Pouch ◽  
Retractor Muscle ◽  
Hamster Cheek Pouch

We present a computer-aided videodensitometric method for the determination of oxygen saturation in red blood cells flowing through capillaries of the hamster cheek pouch retractor muscle. The optical density (OD) of red blood cells is determined at two wavelengths. At the first, 431 nm, there is a maximum difference between absorption by oxygen deoxyhemoglobin. At the second, 420 nm, absorption is equal for the two absorbing species (isosbestic wavelength). In capillaries of the retractor muscle a relationship between oxygen saturation (S) and the following OD ratio was obtained as S = -1.71 (OD431/OD420) + 2.20. The error (95% confidence interval) in oxygen saturation associated with a determination of the OD ratio is estimated to be +/- 4.8%. The computerization of the method employs a frame-by-frame analysis of the light intensity over a selected capillary segment. The light intensity waveform along the segment is digitized and the minimum (I) and maximum (I0) light intensities are used to compute an optical density (OD = log10 [I0/I]). These minimum and maximum intensities correspond to the presence and absence of a red blood cell, respectively. The method permits the off-line analysis of videotaped scenes and provides a means of assessing the extent of temporal and spatial heterogeneity of oxygen saturation in selected capillary networks. The method has been developed for use in capillaries in transilluminated striated muscle but should be generally applicable to the measurement of capillary oxygen saturation in other tissues.

scholarly journals STUDIES ON HEMAGGLUTINATION AND HEMOLYSIS BY ESCHERICHIA COLI ANTISERA

1952 ◽  
Vol 96 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Erwin Neter ◽  
Lee F. Bertram ◽  
Dorothy A. Zak ◽  
Miriam R. Murdock ◽  
Carl E. Arbesman
Keyword(s):  
Escherichia Coli ◽  
Guinea Pig ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Homologous Blood ◽  
E Coli ◽  
Blood Group A ◽  
Human Red Blood Cells ◽  
Order Of Magnitude ◽  
Group A

A study on hemagglutination and hemolysis by Escherichia coli O111 and O55 (rabbit) antisera and on hemagglutination and hemolysis inhibition by E. coli O111 and O55 antigens revealed the following facts. 1. Red blood cells of man, dog, rabbit, guinea pig, sheep, rat, and chicken adsorb E. coli O111 and O55 antigens and thus become specifically agglutinable by the homologous E. coli antisera. 2. The adsorption of these E. coli antigens is a function of the concentration of the antigen, the time (from 5 minutes to 2 hours) of treatment of the red blood cells with the antigen, and the concentration of the red blood cells used. 3. Red blood cells of man and sheep adsorb simultaneously both antigens, as indicated by the fact that both antisera give agglutination of all red blood cells. Complete agglutination does not occur when a mixture of red blood cells treated separately with the two antigens is added to one or the other of the two antisera. 4. Treatment of red blood cells of man with one of the antigens does not block the adsorption of the second antigen. Human cells treated with either or both antigens are still agglutinated by the homologous blood group (A, B, and Rh)-specific antibodies. 5. In the presence of guinea pig complement, E. coli O111 and O55 antisera produce hemolysis of modified human red blood cells in titers of the same order of magnitude as those giving hemagglutination and bacterial agglutination. The same antisera produce hemolysis of sheep cells treated with the identical antigens in titers exceeding by far those giving agglutination of modified human or sheep red blood cells. 6. Both sediment and supernate of a boiled E. coli suspension are capable of modifying red blood cells for E. coli hemagglutination; in contrast, the supernate obtained from an unboiled suspension and then heated does not modify red blood cells for hemagglutination, although it contains the antigen which can specifically adsorb E. coli antibodies, as shown by means of the hemagglutination and hemolysis inhibition tests. 7. Both the unheated and the boiled suspensions of E. coli O111 and O55 inhibit hemagglutination and hemolysis specifically. 8. Rabbit red blood cells modified by either E. coli O111 or 055 antigens, upon intravenous injection into rabbits, engender specific E. coli antibodies. The significance of the results is discussed.

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