Effect of Total Body X-Irradiation on Plasma Volume, Red Cell Volume, Blood Volume and Thiocyanate Space in Normal and Splenectomized Rats

1956 ◽  
Vol 185 (3) ◽  
pp. 446-449 ◽  
Author(s):  
Kee-Chang Huang ◽  
James H. Bondurant
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cell ◽  
Red Cell Volume ◽  
X Ray ◽  
Before And After ◽  
X Irradiation ◽  
Volume Blood ◽  
Total Body

A single dose of 600 r of x-ray was given to groups of nonanesthetized normal and splenectomized rats. Simultaneous estimations of plasma volume, red cell volume, and thiocyanate space were performed in these animals before and after x-irradiation. In normal rats plasma volume decreased in the first 8 days postirradiation but showed a significant increase by the 10th day. The reduction of red cell volume and hematocrit developed gradually and was markedly shown by the 10th day. The blood volume after irradiation was generally larger than that of the normal value. The SCN space increased on the 4th day after irradiation then decreased in the next period and was elevated again by the 10th day postirradiation. In splenectomized rats the change of SCN space was similar to that observed in normal rats and the changes of blood volume were varied. A marked reduction of red cell volume with an increase, probably compensatory, of plasma volume, developed in these rats by the 4th day after x-irradiation.

Blood Volume Measurement in Baboons: Simultaneous Estimation of Red Cell Volume and Plasma Volume

1985 ◽  
Vol 14 (6) ◽  
pp. 345-356
Author(s):  
Michael G. Garner ◽  
Andrew F. Phippard ◽  
John S. Horvath ◽  
Geoffrey G. Duggin ◽  
David J. Tiller
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Volume Measurement ◽  
Simultaneous Estimation ◽  
Red Cell ◽  
Red Cell Volume ◽  
Blood Volume Measurement

Circulating and tissue hematocrits of normal unanesthetized mice

1959 ◽  
Vol 196 (2) ◽  
pp. 420-422 ◽  
Author(s):  
Julius J. Friedman
Keyword(s):  
Serum Albumin ◽  
Cell Volume ◽  
Plasma Volume ◽  
Venous Blood ◽  
Red Cells ◽  
Red Cell ◽  
Volume Data ◽  
Red Cell Volume ◽  
Volume Measurements ◽  
Total Body

The circulating and tissue hematocrits of normal unanesthetized mice were determined by means of independent red cell and plasma volume measurements. The red cell volume-indicator which was used in this study was radioiron (Fe59) tagged red cells. The plasma volume data were derived by means of radioiodine (I131) labeled serum albumin and were reported earlier (Friedman, Proc. Soc. Exper. Biol. & Med. 88: 323, 1955). The hematocrits of the various tissues were found to be: for spleen 51.3, lung 47.9, muscle 49.9, liver 38.9, intestine, 32.2, skin 29.2 and kidney 24.0%. The total body hematocrit was 35.4% as compared to 48.4 for venous blood. All tissues, with the exception of spleen and lung, contained hematocrits which were lower than that of venous blood suggesting the presence of some mechanism within the various tissues which is capable of effectively separating plasma from red cells.

Splenic red cell sequestration and blood volume measurements in conscious pigs

1985 ◽  
Vol 248 (3) ◽  
pp. R293-R301 ◽  
Author(s):  
J. P. Hannon ◽  
C. A. Bossone ◽  
W. G. Rodkey
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Physical Restraint ◽  
Red Cell ◽  
Red Cell Volume ◽  
Epinephrine Injection ◽  
Volume Measurements

When estimated by the dilution of 51Cr-labeled red blood cells under nearly basal conditions, immature splenectomized pigs (n = 20) had a circulating red cell volume of 17.8 +/- 1.64 (SD) ml/kg. At an assumed body-to-large vessel hematocrit (BH:LH) ratio of 0.9, plasma volume was 49.6 +/- 3.12 ml/kg and blood volume 67.3 +/- 3.67 ml/kg. Sham-operated pigs (n = 20) had a circulating red cell volume of 16.2 +/- 1.39 ml/kg, a plasma volume of 51.1 +/- 3.42 ml/kg, and blood volume of 67.2 +/- 4.12 ml/kg. Kinetic analysis of early 51Cr loss from the circulating blood of the sham-operated pigs indicated a splenic red cell sequestration of 4.5 +/- 0.89 ml/kg and a t1/2 of 9.76 +/- 1.93 min for splenic red cell turnover. Epinephrine injection (n = 6) and physical restraint (n = 8) caused rapid mobilization of splenic red blood cells in sham-operated pigs. Volume estimates in splenectomized pigs (n = 7) based on simultaneous dilutions of 51Cr-labeled red blood cells and 125I-labeled bovine albumin gave circulating red cell, plasma, and blood volumes of 18.4 +/- 2.46, 60.7 +/- 4.01, and 79.0 +/- 3.51 ml/kg, respectively, and a BH:LH ratio of 0.756 +/- 0.029. The latter value may have reflected an overestimation of plasma volume by the 125I-labeled albumin procedure.

scholarly journals Blood volume in monoclonal gammopathy

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 301-307 ◽  
Author(s):  
R Alexanian
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Monoclonal Gammopathy ◽  
Red Cell ◽  
Total Blood Volume ◽  
Elevated Plasma ◽  
Total Blood ◽  
Red Cell Volume ◽  
The Relationship

Abstract The plasma volume, red cell volume, or both were measured in 170 normal, anemic, or polycythemic subjects. For anemic subjects without a serum protein abnormality or splenomegaly, the relationship between hematocrit and red cell volume was linear and predictable. In patients with a serum monoclonal globulin on electrophoresis, the plasma voluem was significantly increased for the hematocrit in 30%, and the total blood volume was increased in 45%. The frequency of an elevated plasma volume was higher in patients with a markedly increased level of monoclonal protein. Reductions of abnormal proteins with chemotherapy were associated with declines in plasma volume. For a specific concentration, the serum viscosity was highest in patients with IgM proteins and lowest in patients with IgG globulins. Marked elevations in viscosity were noted only in sera with macroglobulinemia or with more than 5 g/dl of IgG or IgA globulins.

Changes of serum and blood volumes during gestation and lactation in multiparous sows

1996 ◽  
Vol 76 (2) ◽  
pp. 263-266 ◽  
Author(s):  
J. J. Matte ◽  
C. L. Girard
Keyword(s):  
Key Words ◽  
Cell Volume ◽  
Blood Volume ◽  
Red Cell ◽  
Red Cell Volume ◽  
Lactating Sows ◽  
Volume Blood ◽  
Blood Volumes

Changes of serum and plasma volumes were determined in 36 gestating sows and 20 lactating sows at their second parity. There was no change (P > 0.05) in blood and serum volumes between 4 wk pre-mating and 1 wk post-mating. During gestation, blood and serum volumes increased by approximately 25%, with most of this increase occurring between 11 and 14 wk of pregnancy (P < 0.006). From parturition to weaning at 4 wk of lactation, serum and blood volumes decreased linearly (P < 0.02) by approximately 9%. Key words: Serum volume, blood volume, packed red cell volume, sow

Splanchnic blood volume in traumatic shock

1961 ◽  
Vol 200 (3) ◽  
pp. 614-618 ◽  
Author(s):  
J. J. Friedman
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Traumatic Shock ◽  
Red Cell ◽  
Differential Distribution ◽  
Red Cell Volume ◽  
Tourniquet Shock

The distribution of radioiodinated plasma and radioiron-labeled red blood cells between the liver, intestine and spleen were determined during the induction and development of tourniquet shock in mice. The data obtained indicate that plasma and red blood cells are distributed differentially throughout the splanchnic vasculature such that plasma volume of liver, intestine and spleen remain depressed for the entire shock interval, as does splenic red cell volume. After an early decline, the red cell volume of liver and intestine become elevated to a level above control. This differential distribution of plasma and red cells in liver and intestine is attributed to alterations in peripherovascular tone and suggests that a venous component becomes prominent late in shock and may act to pool blood out of active circulation.

Alterations in tissue blood volume induced by tourniquet application

1960 ◽  
Vol 198 (4) ◽  
pp. 886-890 ◽  
Author(s):  
J. J. Friedman
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Peripheral Circulation ◽  
Red Cells ◽  
The Other ◽  
Red Cell ◽  
Red Cell Volume ◽  
Resistance Vessels ◽  
Venous Resistance

The application of occluding tourniquets to both hind legs of unanesthetized mice produced widespread changes in the distribution of plasma and red cells throughout the peripheral circulation. Following tourniquet application the plasma volume within all tissues declined except for lung which remained unaltered and muscle which exhibited an increase. The red cell volume changed variably, declining in liver and spleen, rising in kidney and muscle and remaining unchanged in the other tissues analyzed. These changes were suggestive of a somewhat generalized increase in peripherovascular constrictor activity which included venous resistance vessels in addition to arterioles.

scholarly journals The Blood Volume of Normal Women

Blood ◽  
1954 ◽  
Vol 9 (12) ◽  
pp. 1205-1207 ◽  
Author(s):  
G. R. WADSWORTH
Keyword(s):  
Body Weight ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cell ◽  
Red Cell Volume ◽  
Normal Women ◽  
The Relationship

Abstract Measurements of red cell volume and plasma volume in eight normal women confirm that, in relation to body weight, red cell volume is distinctly lower in women than in men and plasma volume only slightly lower. The relationship between body hematocrit and venous hematocrit (0.90) was found not to be significantly different from that of men.

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