Splenic red cell sequestration and blood volume measurements in conscious pigs

1985 ◽  
Vol 248 (3) ◽  
pp. R293-R301 ◽  
Author(s):  
J. P. Hannon ◽  
C. A. Bossone ◽  
W. G. Rodkey
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Physical Restraint ◽  
Red Cell ◽  
Red Cell Volume ◽  
Epinephrine Injection ◽  
Volume Measurements

When estimated by the dilution of 51Cr-labeled red blood cells under nearly basal conditions, immature splenectomized pigs (n = 20) had a circulating red cell volume of 17.8 +/- 1.64 (SD) ml/kg. At an assumed body-to-large vessel hematocrit (BH:LH) ratio of 0.9, plasma volume was 49.6 +/- 3.12 ml/kg and blood volume 67.3 +/- 3.67 ml/kg. Sham-operated pigs (n = 20) had a circulating red cell volume of 16.2 +/- 1.39 ml/kg, a plasma volume of 51.1 +/- 3.42 ml/kg, and blood volume of 67.2 +/- 4.12 ml/kg. Kinetic analysis of early 51Cr loss from the circulating blood of the sham-operated pigs indicated a splenic red cell sequestration of 4.5 +/- 0.89 ml/kg and a t1/2 of 9.76 +/- 1.93 min for splenic red cell turnover. Epinephrine injection (n = 6) and physical restraint (n = 8) caused rapid mobilization of splenic red blood cells in sham-operated pigs. Volume estimates in splenectomized pigs (n = 7) based on simultaneous dilutions of 51Cr-labeled red blood cells and 125I-labeled bovine albumin gave circulating red cell, plasma, and blood volumes of 18.4 +/- 2.46, 60.7 +/- 4.01, and 79.0 +/- 3.51 ml/kg, respectively, and a BH:LH ratio of 0.756 +/- 0.029. The latter value may have reflected an overestimation of plasma volume by the 125I-labeled albumin procedure.

Splanchnic blood volume in traumatic shock

1961 ◽  
Vol 200 (3) ◽  
pp. 614-618 ◽  
Author(s):  
J. J. Friedman
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Traumatic Shock ◽  
Red Cell ◽  
Differential Distribution ◽  
Red Cell Volume ◽  
Tourniquet Shock

The distribution of radioiodinated plasma and radioiron-labeled red blood cells between the liver, intestine and spleen were determined during the induction and development of tourniquet shock in mice. The data obtained indicate that plasma and red blood cells are distributed differentially throughout the splanchnic vasculature such that plasma volume of liver, intestine and spleen remain depressed for the entire shock interval, as does splenic red cell volume. After an early decline, the red cell volume of liver and intestine become elevated to a level above control. This differential distribution of plasma and red cells in liver and intestine is attributed to alterations in peripherovascular tone and suggests that a venous component becomes prominent late in shock and may act to pool blood out of active circulation.

Effect of polycythemia on vascular volume in the newborn dog

1984 ◽  
Vol 246 (6) ◽  
pp. H830-H837
Author(s):  
M. H. Leblanc ◽  
K. Pate
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Evans Blue ◽  
Whole Blood ◽  
Blood Cells ◽  
Exchange Transfusion ◽  
Red Cell ◽  
Vascular Volume

The effect of polycythemia [hematocrit (Hct) 64-80] on blood volume (BV) was studied in 27 unanesthetized, splenectomized newborn dogs (age 6-14 days, postsplenectomy 5-13 days). Normovolemic polycythemia (N) was induced in nine pups by exchange transfusion with 75 ml/kg of adult, packed (to Hct 95) red blood cells (RBC). Hypervolemic polycythemia (H) was induced in 11 pups by transfusion of RBC (50 ml/kg). Seven pups received exchange transfusion with 75 ml/kg of whole blood and served as controls (C). Red cell volume (RCV, 51CrRBC) and plasma volume (PV, 125I-fibrinogen and Evans blue) were measured prior to and at 1, 2, and 4 h after transfusion, before the pups received fluid orally. The pups were fed 8 ml X kg-1 X h-1 after 4 h, and measurements were repeated at 8 and 24 h. BV fell in C prior to 4 h by 10 +/- 4% (SD) (P less than 0.01) and then rose to initial levels. BV rose in the N pups by 17 +/- 9 (P less than 0.01), 14 +/- 5 (P less than 0.01), 9 +/- 10 (P less than 0.1), 17 +/- 9 (P less than 0.01), and 31 +/- 17% (P less than 0.01) at 1, 2, 4, 8, and 24 h post transfusion. BV rose in the H pups by 41 +/- 8, 35 +/- 10, 23 +/- 11, 27 +/- 6, and 43 +/- 9% (all P less than 0.01). Thus newborn dogs with induced N or H equilibrate rapidly to a BV significantly higher than C levels.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Volume Measurement in Baboons: Simultaneous Estimation of Red Cell Volume and Plasma Volume

1985 ◽  
Vol 14 (6) ◽  
pp. 345-356
Author(s):  
Michael G. Garner ◽  
Andrew F. Phippard ◽  
John S. Horvath ◽  
Geoffrey G. Duggin ◽  
David J. Tiller
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Volume Measurement ◽  
Simultaneous Estimation ◽  
Red Cell ◽  
Red Cell Volume ◽  
Blood Volume Measurement

PLACENTAL TRANSFUSION AND HYPERBILIRUBINEMIA IN THE PREMATURE

PEDIATRICS ◽  
1972 ◽  
Vol 49 (3) ◽  
pp. 406-419 ◽  
Author(s):  
Saroj Saigal ◽  
Allison O'Neill ◽  
Yeldandi Surainder ◽  
Le-Beng Chua ◽  
Robert Usher
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Premature Infants ◽  
Full Term ◽  
Neonatal Hyperbilirubinemia ◽  
Red Cell ◽  
Term Infants ◽  
Red Cell Volume ◽  
Cord Clamping ◽  
Volume Measurements

Placental transfusion has been compared in premature and full-term infants. Blood volume measurements showed that the 5-minute transfusion was similar in full-term and premature infants (47% and 50% increase in blood volume from birth). A larger proportion of the 5-minute transfusion occurred by 1 minute in full-term (76%) than in premature infants (56%). Placental transfusion, by increasing red cell volume, greatly enhanced the severity of neonatal hyperbilirubinemia. Bilirubin concentrations of 15 mg/100 ml developed in only 6% of premature infants when cord clamping was immediate, in 14% when cord clamping was delayed 1 minute, and in 38% after a 5-minute delay in cord clamping.

Circulating and tissue hematocrits of normal unanesthetized mice

1959 ◽  
Vol 196 (2) ◽  
pp. 420-422 ◽  
Author(s):  
Julius J. Friedman
Keyword(s):  
Serum Albumin ◽  
Cell Volume ◽  
Plasma Volume ◽  
Venous Blood ◽  
Red Cells ◽  
Red Cell ◽  
Volume Data ◽  
Red Cell Volume ◽  
Volume Measurements ◽  
Total Body

The circulating and tissue hematocrits of normal unanesthetized mice were determined by means of independent red cell and plasma volume measurements. The red cell volume-indicator which was used in this study was radioiron (Fe59) tagged red cells. The plasma volume data were derived by means of radioiodine (I131) labeled serum albumin and were reported earlier (Friedman, Proc. Soc. Exper. Biol. & Med. 88: 323, 1955). The hematocrits of the various tissues were found to be: for spleen 51.3, lung 47.9, muscle 49.9, liver 38.9, intestine, 32.2, skin 29.2 and kidney 24.0%. The total body hematocrit was 35.4% as compared to 48.4 for venous blood. All tissues, with the exception of spleen and lung, contained hematocrits which were lower than that of venous blood suggesting the presence of some mechanism within the various tissues which is capable of effectively separating plasma from red cells.

scholarly journals Blood volume in monoclonal gammopathy

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 301-307 ◽  
Author(s):  
R Alexanian
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Monoclonal Gammopathy ◽  
Red Cell ◽  
Total Blood Volume ◽  
Elevated Plasma ◽  
Total Blood ◽  
Red Cell Volume ◽  
The Relationship

Abstract The plasma volume, red cell volume, or both were measured in 170 normal, anemic, or polycythemic subjects. For anemic subjects without a serum protein abnormality or splenomegaly, the relationship between hematocrit and red cell volume was linear and predictable. In patients with a serum monoclonal globulin on electrophoresis, the plasma voluem was significantly increased for the hematocrit in 30%, and the total blood volume was increased in 45%. The frequency of an elevated plasma volume was higher in patients with a markedly increased level of monoclonal protein. Reductions of abnormal proteins with chemotherapy were associated with declines in plasma volume. For a specific concentration, the serum viscosity was highest in patients with IgM proteins and lowest in patients with IgG globulins. Marked elevations in viscosity were noted only in sera with macroglobulinemia or with more than 5 g/dl of IgG or IgA globulins.

scholarly journals Blood volume, plasma volume and circulation time in a high-energy-demand teleost, the yellowfin tuna (Thunnus albacares)

1998 ◽  
Vol 201 (5) ◽  
pp. 647-654 ◽  
Author(s):  
R Brill ◽  
K Cousins ◽  
D Jones ◽  
P G Bushnell ◽  
J F Steffensen
Keyword(s):  
Red Blood Cells ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Circulatory System ◽  
Yellowfin Tuna ◽  
Circulation Time ◽  
Whole Body ◽  
Thunnus Albacares ◽  
Red Cell

We measured red cell space with 51Cr-labeled red blood cells, and dextran space with 500 kDa fluorescein-isothiocyanate-labeled dextran (FITC-dextran), in two groups of yellowfin tuna (Thunnus albacares). Red cell space was 13.8+/-0.7 ml kg-1 (mean +/- s.e.m.) Assuming a whole-body hematocrit equal to the hematocrit measured at the ventral aortic sampling site and no significant sequestering of 51Cr-labeled red blood cells by the spleen, blood volume was 46. 7+/-2.2 ml kg-1. This is within the range reported for most other teleosts (30-70 ml kg-1), but well below that previously reported for albacore (Thunnus alalunga, 82-197 ml kg-1). Plasma volume within the primary circulatory system (calculated from the 51Cr-labeled red blood cell data) was 32.9+/-2.3 ml kg-1. Dextran space was 37.0+/-3.7 ml kg-1. Because 500 kDa FITC-dextran appeared to remain within the vascular space, these data imply that the volume of the secondary circulatory system of yellowfin tuna is small, and its exact volume is not measurable by our methods. Although blood volume is not exceptional, circulation time (blood volume/cardiac output) is clearly shorter in yellowfin tuna than in other active teleosts. In a 1 kg yellowfin tuna, circulation time is approximately 0.4 min (47 ml kg-1/115 ml min-1 kg-1) compared with 1. 3 min (46 ml kg-1/35 ml min-1 kg-1) in yellowtail (Seriola quinqueradiata) and 1.9 min (35 ml kg-1/18 ml min-1 kg-1) in rainbow trout (Oncorhynchus mykiss). In air-breathing vertebrates, high metabolic rates are necessarily correlated with short circulation times. Our data are the first to imply that a similar relationship occurs in fishes.

Sign in / Sign up

Export Citation Format

Share Document