Formulae for Direct Calculation of Red Cell Volume, Plasma Volume and Blood Volume with the Aid of the Ashby Technic

2009 ◽  
Vol 164 (3) ◽  
pp. 253-256 ◽  
Author(s):  
RAGNAR BERLIN
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Direct Calculation ◽  
Red Cell ◽  
Red Cell Volume

Blood Volume Measurement in Baboons: Simultaneous Estimation of Red Cell Volume and Plasma Volume

1985 ◽  
Vol 14 (6) ◽  
pp. 345-356
Author(s):  
Michael G. Garner ◽  
Andrew F. Phippard ◽  
John S. Horvath ◽  
Geoffrey G. Duggin ◽  
David J. Tiller
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Volume Measurement ◽  
Simultaneous Estimation ◽  
Red Cell ◽  
Red Cell Volume ◽  
Blood Volume Measurement

Splenic red cell sequestration and blood volume measurements in conscious pigs

1985 ◽  
Vol 248 (3) ◽  
pp. R293-R301 ◽  
Author(s):  
J. P. Hannon ◽  
C. A. Bossone ◽  
W. G. Rodkey
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Physical Restraint ◽  
Red Cell ◽  
Red Cell Volume ◽  
Epinephrine Injection ◽  
Volume Measurements

When estimated by the dilution of 51Cr-labeled red blood cells under nearly basal conditions, immature splenectomized pigs (n = 20) had a circulating red cell volume of 17.8 +/- 1.64 (SD) ml/kg. At an assumed body-to-large vessel hematocrit (BH:LH) ratio of 0.9, plasma volume was 49.6 +/- 3.12 ml/kg and blood volume 67.3 +/- 3.67 ml/kg. Sham-operated pigs (n = 20) had a circulating red cell volume of 16.2 +/- 1.39 ml/kg, a plasma volume of 51.1 +/- 3.42 ml/kg, and blood volume of 67.2 +/- 4.12 ml/kg. Kinetic analysis of early 51Cr loss from the circulating blood of the sham-operated pigs indicated a splenic red cell sequestration of 4.5 +/- 0.89 ml/kg and a t1/2 of 9.76 +/- 1.93 min for splenic red cell turnover. Epinephrine injection (n = 6) and physical restraint (n = 8) caused rapid mobilization of splenic red blood cells in sham-operated pigs. Volume estimates in splenectomized pigs (n = 7) based on simultaneous dilutions of 51Cr-labeled red blood cells and 125I-labeled bovine albumin gave circulating red cell, plasma, and blood volumes of 18.4 +/- 2.46, 60.7 +/- 4.01, and 79.0 +/- 3.51 ml/kg, respectively, and a BH:LH ratio of 0.756 +/- 0.029. The latter value may have reflected an overestimation of plasma volume by the 125I-labeled albumin procedure.

scholarly journals Blood volume in monoclonal gammopathy

Blood ◽  
1977 ◽  
Vol 49 (2) ◽  
pp. 301-307 ◽  
Author(s):  
R Alexanian
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Monoclonal Gammopathy ◽  
Red Cell ◽  
Total Blood Volume ◽  
Elevated Plasma ◽  
Total Blood ◽  
Red Cell Volume ◽  
The Relationship

Abstract The plasma volume, red cell volume, or both were measured in 170 normal, anemic, or polycythemic subjects. For anemic subjects without a serum protein abnormality or splenomegaly, the relationship between hematocrit and red cell volume was linear and predictable. In patients with a serum monoclonal globulin on electrophoresis, the plasma voluem was significantly increased for the hematocrit in 30%, and the total blood volume was increased in 45%. The frequency of an elevated plasma volume was higher in patients with a markedly increased level of monoclonal protein. Reductions of abnormal proteins with chemotherapy were associated with declines in plasma volume. For a specific concentration, the serum viscosity was highest in patients with IgM proteins and lowest in patients with IgG globulins. Marked elevations in viscosity were noted only in sera with macroglobulinemia or with more than 5 g/dl of IgG or IgA globulins.

Splanchnic blood volume in traumatic shock

1961 ◽  
Vol 200 (3) ◽  
pp. 614-618 ◽  
Author(s):  
J. J. Friedman
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Blood Cells ◽  
Traumatic Shock ◽  
Red Cell ◽  
Differential Distribution ◽  
Red Cell Volume ◽  
Tourniquet Shock

The distribution of radioiodinated plasma and radioiron-labeled red blood cells between the liver, intestine and spleen were determined during the induction and development of tourniquet shock in mice. The data obtained indicate that plasma and red blood cells are distributed differentially throughout the splanchnic vasculature such that plasma volume of liver, intestine and spleen remain depressed for the entire shock interval, as does splenic red cell volume. After an early decline, the red cell volume of liver and intestine become elevated to a level above control. This differential distribution of plasma and red cells in liver and intestine is attributed to alterations in peripherovascular tone and suggests that a venous component becomes prominent late in shock and may act to pool blood out of active circulation.

Alterations in tissue blood volume induced by tourniquet application

1960 ◽  
Vol 198 (4) ◽  
pp. 886-890 ◽  
Author(s):  
J. J. Friedman
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Peripheral Circulation ◽  
Red Cells ◽  
The Other ◽  
Red Cell ◽  
Red Cell Volume ◽  
Resistance Vessels ◽  
Venous Resistance

The application of occluding tourniquets to both hind legs of unanesthetized mice produced widespread changes in the distribution of plasma and red cells throughout the peripheral circulation. Following tourniquet application the plasma volume within all tissues declined except for lung which remained unaltered and muscle which exhibited an increase. The red cell volume changed variably, declining in liver and spleen, rising in kidney and muscle and remaining unchanged in the other tissues analyzed. These changes were suggestive of a somewhat generalized increase in peripherovascular constrictor activity which included venous resistance vessels in addition to arterioles.

scholarly journals The Blood Volume of Normal Women

Blood ◽  
1954 ◽  
Vol 9 (12) ◽  
pp. 1205-1207 ◽  
Author(s):  
G. R. WADSWORTH
Keyword(s):  
Body Weight ◽  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cell ◽  
Red Cell Volume ◽  
Normal Women ◽  
The Relationship

Abstract Measurements of red cell volume and plasma volume in eight normal women confirm that, in relation to body weight, red cell volume is distinctly lower in women than in men and plasma volume only slightly lower. The relationship between body hematocrit and venous hematocrit (0.90) was found not to be significantly different from that of men.

scholarly journals Blood volume and hemoglobin mass in long-term heart transplant recipients with and without Anemia

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Florian Seiler ◽  
Christoph Ahlgrim ◽  
Philipp Birkner ◽  
Nina Wrobel ◽  
Jonathan Rilinger ◽  
...  
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Heart Transplant ◽  
Control Group ◽  
Red Cell ◽  
Transplant Recipients ◽  
Red Cell Volume ◽  
Heart Transplant Recipients

Abstract Background In systolic chronic heart failure, a heterogeneous blood volume (BV) regulation can be found with plasma volume expansion in many cases, possibly leading to pseudoanemia. Little is known about the volume status after heart transplantation (HTX). So far, anemia of HTX recipients was solely investigated using hemoglobin-concentration that may be misleading in a clinical context. The objective of the study was whether a difference in plasma volume and red cell volume can be observed in clinically stable heart transplant recipients compared to matched control subjects. Secondary, the aim was to describe anemia in the long-term after HTX based on quantitative data. Methods Blood volume and its constituents red cell volume and plasma volume were quantified using an abbreviated carbon monoxide rebreathing method (aCORM) with focus on its primary measure total hemoglobin mass (Hbmass) and coincidental anemia in 36 (7 women) heart transplant recipients. For comparison, a matched control group of 46 (5 women) healthy subjects was selected. Results Neither Hbmass nor blood volumes were significantly different in HTX patients compared to matched healthy control group subjects. The prevalence of anemia 6.3 ± 4.3 years after transplantation was 19%. Hbmass and red cell volume were significantly lower in anemic HTX patients compared to non-anemic patients while plasma volume was not expanded. Various immunosuppressant regimens did not have an effect on Hbmass, plasma volume or red cell volume. Conclusions There was no difference in blood volumes and Hbmass between HTX patients and control subjects. The pathophysiologic blood volume regulation in chronic heart failure does not seem to be longer active in long-term HTX recipients. However, in the long-term after HTX, anemia occurs in a considerable number of patients as true anemia without a clear association with immunosuppression. Trial registration German registry for clinical studies, DRKS00006078. Registered 09 May 2014, https://www.drks.de/drks_web/navigate.do?navigationId=trial. HTML&TRIAL_ID=DRKS00006078.

Role of spleen in ANF-induced reduction in plasma volume

1992 ◽  
Vol 70 (8) ◽  
pp. 1104-1108 ◽  
Author(s):  
Susan Kaufman
Keyword(s):  
Cell Volume ◽  
Blood Volume ◽  
Plasma Volume ◽  
Atrial Natriuretic Factor ◽  
Saline Control ◽  
Red Cell ◽  
Red Cell Volume ◽  
Important Site ◽  
Intact Rats

Atrial natriuretic factor (ANF) causes an increase in hematocrit that cannot be accounted for by urinary losses. The mechanism behind this phenomenon was studied in intact and splenectomized rats. Rat ANF 99–126 was infused i.v. for 30 min into conscious rats at rates of 0 (saline control), 0.05, or 0.1 μg/min. Plasma volume was then determined by dilution of the dye, Evan's Blue. In one group of rats, red cell volume was determined using 51Cr-labelled erythrocytes. ANF infusion was continued uninterrupted throughout the experiments. In the intact rats, ANF (0.10 μg/min) caused hematocrit to increase from 38.9 ± 0.5 to 41.2 ± 0.4% (p < 0.005). Splenectomy so attenuated this response to ANF that it failed to reach significance. Similarly, ANF (0.10 μg/min) caused plasma volume to fall from 5.1 ± 0.1 to 4.5 ± 0.1 mL/100 g body wt. (p < 0.005) in the intact rats, but did not affect plasma volume in the splenectomized rats. As a result, blood volume was significantly reduced by ANF in the intact rats, but remained unchanged in the splenectomized rats. Red cell volume did not change in response to infusion of ANF, nor did ANF affect the rate of clearance of Evan's Blue out of the plasma. It is concluded that the spleen is an important site of movement of protein-poor fluid out of the vasculature, and that this exchange is influenced by ANF.Key words: spleen, ANF, blood volume, hematocrit.

Simultaneous determination of blood volume with Cr51 and T-1824 during hypothermia and rewarming

1959 ◽  
Vol 196 (4) ◽  
pp. 703-705 ◽  
Author(s):  
E. J. Fedor ◽  
B. Fisher
Keyword(s):  
Cell Volume ◽  
Simultaneous Determination ◽  
Blood Volume ◽  
Plasma Volume ◽  
Red Cell ◽  
Total Blood Volume ◽  
Total Blood ◽  
Red Cell Volume ◽  
Usual Feature

Dogs lightly anesthetized with ether, maintained between 23–24°C for 2 hours and rewarmed, were subjected to simultaneous determinations of red cell volume (Cr51) and plasma volume (T-1824). Red cell volume values were unchanged during the course of the experiment. Plasma volumes were significantly decreased during hypothermia and were transiently elevated during rewarming. Twenty-four hours after rewarming, total blood volume and plasma volume values were not significantly different from control values. It would seem that circulatory failure (‘rewarming shock’) is not a usual feature of rewarming following hypothermia of 2 hours duration.

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