Lipolytic action of epinephrine in adipose tissue homogenates

1964 ◽  
Vol 206 (1) ◽  
pp. 149-152 ◽  
Author(s):  
David Rubinstein ◽  
Sylvester Chiu ◽  
Jean Naylor ◽  
John C. Beck
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipolytic Activity ◽  
Supernatant Fraction ◽  
Lipid Layer ◽  
Lipolytic Action ◽  
Tissue Homogenates ◽  
Stimulation Of

Incubation of intact adipose tissue with ACTH, certain preparations of growth hormone, and epinephrine induces an increase in lipolytic activity in homogenates prepared from this tissue. Epinephrine also causes an increase in lipolytic activity when added directly to adipose tissue homogenates. The increase is inhibited by 4 x 10–4 m iodoacetate. Mitochondrial and "supernatant" fractions (including microsomes) and the lipid layer all possess lipase activity, but only the lipase found in the supernatant fraction can be activated by epinephrine. Protamine activates this lipase; addition of epinephrine has no additive effect. Heparin inhibits the stimulation of lipase activity by epinephrine, but has no action on the lipolysis occurring in the absence of epinephrine. It is concluded that the epinephrine-stimulated lipase is distinct from lipoprotein lipase and the lipase present in unstimulated tissue.

Growth hormone inhibits lipoprotein lipase activity in human adipose tissue.

1995 ◽  
Vol 80 (3) ◽  
pp. 936-941 ◽  
Author(s):  
M Ottosson ◽  
K Vikman-Adolfsson ◽  
S Enerbäck ◽  
A Elander ◽  
P Björntorp ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Human Adipose Tissue ◽  
Lipoprotein Lipase Activity

Modifications of Plasma Post-Heparin Lipolytic Activity and Tissue Lipoprotein Lipase Activity Induced in the Rat by Acute Administration of Ethanol or Propan-2-ol

1975 ◽  
Vol 48 (2) ◽  
pp. 153-156
Author(s):  
Y. Giudicelli ◽  
R. Nordmann ◽  
J. Nordmann
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipolytic Activity ◽  
Ethanol Administration ◽  
Acute Administration ◽  
Post Heparin Lipolytic Activity ◽  
Adipose Tissue Lipoprotein Lipase ◽  
Made In

1. The oral administration of propan-2-ol [isopropanol; 100 mmol (6 g)/kg body weight] or ethanol [130 mmol (6 g)/kg body weight] to starved rats produced no change in plasma post-heparin lipase activity (PHLA) compared with that observed in 154 mmol/l sodium chloride (saline)-treated rats. 2. An increase of adipose tissue lipoprotein lipase (LLA) and a decrease of heart LLA occurred in isopropanol-treated animals, whereas no significant changes were found in these activities after ethanol administration. 3. Since administration of isopropanol produces hyperglycaemia, observations were also made in rats receiving glucose infusion rather than saline. In these animals a rise in PHLA and adipose tissue LLA, and a fall in heart LLA, occurred. 4. It is suggested that the changes in tissue LLA produced by isopropanol are mediated by the rise in blood glucose.

Modulation by phenobarbital of lipolytic activity in postheparin plasma and tissues of the rat

1982 ◽  
Vol 60 (11) ◽  
pp. 1077-1083 ◽  
Author(s):  
David M. Goldberg ◽  
M. Waheed Roomi ◽  
Alex Yu
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipolytic Activity ◽  
Sodium Dodecyl ◽  
Male Rats ◽  
Lipoprotein Lipase Activity ◽  
Protamine Sulphate ◽  
Triacylglycerol Lipase ◽  
Postheparin Lipolytic Activity

Male rats injected with phenobarbital at a dose of 100 mg/kg for 5 days manifested increased postheparin lipolytic activity of fasting plasma. Inhibition studies with protamine sulphate, 1 M NaCl, and sodium dodecyl sulphate revealed that the activities of both lipoprotein lipase and hepatic triacylglycerol lipase were increased in the postheparin plasma of the drug-treated rats. Adipose tissue lipoprotein lipase activity was also increased in the phenobarbital-treated rats. The triacylglycerol lipase activity elutable by heparin from liver slices and the residual activity of liver microsomes increased significantly in the drug-treated rats. Lipoprotein lipase of cardiac muscle and red skeletal muscle was unaltered by phenobarbital treatment. The increased postheparin lipolytic activity of fasting phenobarbital-treated rats seems to be accountable through increased lipoprotein lipase activity of adipose tissue and increased triacylglycerol lipase activity of liver, both of which may contribute to the lowered fasting concentrations of serum triacylglycerol mediated by the drug, as previously reported.

Stimulation of lipoprotein lipase activity of rat adipose tissue and post-heparin plasma by N 6 -(phenylisopropyl)adenosine

FEBS Letters ◽  
1981 ◽  
Vol 132 (1) ◽  
pp. 121-123 ◽  
Author(s):  
Jorma J. Ohisalo ◽  
Håkan Strandberg ◽  
Ella Kostiainen ◽  
Timo Kuusi ◽  
Christian Ehnholm
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipoprotein Lipase Activity ◽  
Heparin Plasma ◽  
Rat Adipose Tissue ◽  
Phenylisopropyl Adenosine ◽  
Stimulation Of

THE EFFECT OF INCUBATION ON THE CHARACTERISTICS OF THE LIPOLYTIC ACTIVITY OF RAT ADIPOSE TISSUE

1962 ◽  
Vol 40 (6) ◽  
pp. 703-707 ◽  
Author(s):  
C. H. Hollenberg
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Phosphate Buffer ◽  
Lipolytic Activity ◽  
Serum Triglyceride ◽  
Fresh Tissue ◽  
Intact Tissue ◽  
Separate Type ◽  
Tissue Homogenates ◽  
Rat Adipose Tissue

A study has been made of the changes produced in the characteristics of the lipolytic activity of rat adipose tissue homogenates by prolonged preincubation of the intact tissue in phosphate buffer. Fresh tissue homogenates were markedly more active on a serum–triglyceride medium (activated substrate) than on triglyceride alone while little difference was noted with homogenates of preincubated tissue. The activity of fresh tissue homogenates was increased by addition of heparin; homogenates of incubated tissue were not affected. Fresh tissue homogenates were inhibited to a greater extent by protamine than were preparations of incubated material. Addition of glucose and insulin to the buffer partially maintained the activity of incubated tissue on activated substrate. These results indicate that while lipolytic activity of fresh rat adipose tissue has characteristics similar to lipoprotein lipase, the activity of incubated tissue differs from this enzyme in several respects. This study demonstrates the lability of lipoprotein lipase in rat adipose tissue and suggests the presence in this tissue of a separate type of lipolytic activity.

THE EFFECT OF INCUBATION ON THE CHARACTERISTICS OF THE LIPOLYTIC ACTIVITY OF RAT ADIPOSE TISSUE

1962 ◽  
Vol 40 (1) ◽  
pp. 703-707
Author(s):  
C. H. Hollenberg
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Phosphate Buffer ◽  
Lipolytic Activity ◽  
Serum Triglyceride ◽  
Fresh Tissue ◽  
Intact Tissue ◽  
Separate Type ◽  
Tissue Homogenates ◽  
Rat Adipose Tissue

A study has been made of the changes produced in the characteristics of the lipolytic activity of rat adipose tissue homogenates by prolonged preincubation of the intact tissue in phosphate buffer. Fresh tissue homogenates were markedly more active on a serum–triglyceride medium (activated substrate) than on triglyceride alone while little difference was noted with homogenates of preincubated tissue. The activity of fresh tissue homogenates was increased by addition of heparin; homogenates of incubated tissue were not affected. Fresh tissue homogenates were inhibited to a greater extent by protamine than were preparations of incubated material. Addition of glucose and insulin to the buffer partially maintained the activity of incubated tissue on activated substrate. These results indicate that while lipolytic activity of fresh rat adipose tissue has characteristics similar to lipoprotein lipase, the activity of incubated tissue differs from this enzyme in several respects. This study demonstrates the lability of lipoprotein lipase in rat adipose tissue and suggests the presence in this tissue of a separate type of lipolytic activity.

Post-Heparin Lipolytic Activity and Tissue Lipoprotein Lipase Activity in the Alloxan-Diabetic Rat

1974 ◽  
Vol 46 (5) ◽  
pp. 661-664
Author(s):  
R. S. Elkeles ◽  
E. Williams
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipolytic Activity ◽  
Diabetic Rats ◽  
Plasma Triglyceride ◽  
Diabetic Rat ◽  
Lipoprotein Lipase Activity ◽  
Post Heparin Lipolytic Activity ◽  
Adipose Tissue Lipoprotein Lipase

1. Alloxan-diabetic rats showed raised plasma triglyceride levels and low adipose tissue lipoprotein lipase activity compared with controls. Heart lipoprotein lipase activity appeared unaltered by the diabetic state. 2. Plasma post-heparin lipolytic activity was slightly but not significantly increased in the diabetic group. The significance of these findings is discussed.

Sign in / Sign up

Export Citation Format

Share Document