Separation of pepsinogen II and pepsinogen III from human urine

1964 ◽  
Vol 206 (5) ◽  
pp. 1106-1110 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Proteolytic Activity ◽  
Human Urine ◽  
Chromatographic Behavior ◽  
Pepsinogen I ◽  
Qualitative And Quantitative ◽  
Quantitative Correlation ◽  
Diethylaminoethyl Cellulose ◽  
Pepsinogen Ii ◽  
Urine Specimens

Human urine has been fractionated on diethylaminoethyl cellulose to yield two pepsinogens. They have been called pepsinogen II and pepsinogen III on the basis of chromatographic behavior identical to that of fundic mucosal pepsinogens II and III. Pepsinogen I has been found absent from 23 urine specimens of 12 individuals. Results suggest a gross qualitative and quantitative correlation between pepsinogen II-pepsinogen III ratio (in terms of total proteolytic activity) in urine and gastric mucosa.

Separation of pepsin I, pepsin II A, pepsin II B, and pepsin III from human gastric mucosa

1963 ◽  
Vol 205 (6) ◽  
pp. 1099-1105 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Proteolytic Enzymes ◽  
Low Ph ◽  
Human Gastric Mucosa ◽  
Pepsinogen I ◽  
Fundic Mucosa ◽  
Proximal Duodenum ◽  
Diethylaminoethyl Cellulose ◽  
Pepsinogen Ii

Extracts of human gastric mucosa have been fractionated on diethylaminoethyl cellulose to yield three precursors of proteolytic enzymes active at low pH which have been called pepsinogen I, pepsinogen II, and pepsinogen III. Pepsinogen I has been found in the mucosa from all parts of the stomach examined as well as in the proximal duodenum. Pepsinogen II and pepsinogen III were present in fundic mucosa only.

Separation of pepsinogen I, pepsinogen II, and pepsinogen III from human gastric mucosa

1963 ◽  
Vol 205 (6) ◽  
pp. 1106-1112 ◽  
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Duodenal Mucosa ◽  
Human Gastric Mucosa ◽  
Chromatographic Mobility ◽  
Pepsinogen I ◽  
Fundic Mucosa ◽  
Inhibitor Complex ◽  
Diethylaminoethyl Cellulose ◽  
Pepsinogen Ii ◽  
Pepsin Inhibitor

Extracts of acidified human gastric fundic mucosa have been fractionated on diethylaminoethyl cellulose to yield three pepsin fractions, which have, in order of their elution, been attributed to pepsin I, pepsin II A, and a mixture of pepsin II B and pepsin III. Acidification of previously purified pepsinogen I and pepsinogen III yields pepsin I and pepsin III, respectively. Acidification of previously purified pepsinogen II yields two distinct pepsin fractions, pepsin II A and pepsin II B. Pepsin II B and pepsin III have the same chromatographic mobility on diethylaminoethyl cellulose, but are probably not identical. Pyloric and duodenal mucosa yield only pepsin I upon acidification. Human pepsin-pepsin inhibitor complex is demonstrable as a chromatographic fraction distinct from pepsins upon fractionation of acidified dilute pepsinogen solutions with diethylaminoethyl cellulose equilibrated with acetate buffer pH 5.3.

scholarly journals Separation of pepsinogen I, pepsinogen II, and pepsinogen III from human gastric mucosa

1964 ◽  
Vol 207 (6) ◽  
pp. 1451-1451
Author(s):  
Max J. Seijffers ◽  
Harry L. Segal ◽  
Leon L. Miller
Keyword(s):  
Gastric Mucosa ◽  
Companion Paper ◽  
Human Gastric Mucosa ◽  
Pepsinogen I ◽  
Volume Table ◽  
Pepsinogen Ii

Page 1106: Max J. Seijffers, Harry L. Segal, and Leon L. Miller. "Separation of pepsinogen I, pepsinogen II, and pepsinogen III from human gastric mucosa." Title in abstract, in issue table of contents, and in volume table of contents printed in error as title of companion paper which begins on page 1099. Should read the same as above title.

scholarly journals Non-invasive laboratory markers assessing efficacy of Helicobacter pylori eradication therapy

2019 ◽  
Vol 9 (3-4) ◽  
pp. 523-530
Author(s):  
L. B. Drygina ◽  
V. N. Ellenidi ◽  
N. A. Bardysheva ◽  
M. M. Bogoslovskiy
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Eradication Therapy ◽  
Helicobacter Pylori Infection ◽  
Igg Antibodies ◽  
Ki 67 ◽  
Helicobacter Pylori Eradication ◽  
Pepsinogen I ◽  
Non Invasive ◽  
Pepsinogen Ii

Effective eradication of Helicobacter pylori infection is an important means to reduce the risk of precancerous changes in the gastric mucosa and prevention of gastric cancer. A search for non-invasive diagnostic tools for Helicobacter pylori infection, evaluation of the effectiveness of eradication remains of high importance.The aim of the study was to assess an informative value of detecting pepsinogen I and II as well as serum antibodies to Helicobacter pylori while assessing an efficacy of treated chronic Helicobacter gastritis and identifying preneoplastic changes in the stomach mucosa. There enrolled 113 male patients with chronic gastritis aged 41 to 76, average age- (56.7±0.7) years. Examination of patients was carried out at admission to the clinic, as well as at 2 and 12 months after administering a standard eradication therapy. It was found that Helicobacter pylori infection was detected in 101 (89.4%) patients. Groups of patients with effective eradication therapy were noted. A time-dependent level of antibodies to Helicobacter pylori, as well as measured concentration of pepsinogen I and II after the onset of eradication treatment were determined. Which were analyzed in connection with the results of histology examination of gastric mucosa biopsy specimens and expression of oncoproteins Ki-67, Bcl-2, c-erbB-2, p16 in the gastric mucosa depending on efficacy of eradication therapy. It is shown that effective eradication therapy was characterized by significantly decreased serum level of IgG antibodies to Helicobacter pylori 2 months after the onset of treatment. Moreover, a significantly decreased pepsinogen II and serum IgG antibodies to Helicobacter pylori during eradication therapy were accompanied by a significant decrease in Ki-67 expression in the gastric epithelium. Decreased concentration of pepsinogen II within the first year after Helicobacter pylori eradication therapy was due to a greater decrease in activity of inflammatory changes in the gastric mucosa than to dynamic changes in gastric atrophy and metaplasia. An inverse relation between the serum level of pepsinogen I and atrophy as well as intestinal metaplasia within the gastric mucosa were found.

Helicobacter pylori infection: place of serological and cultural diagnostics in clinical guidelines

2021 ◽  
Vol 66 (8) ◽  
pp. 496-501
Author(s):  
L. A. Kornoukhova ◽  
V. L. Emanuel ◽  
N. L. Denisov ◽  
E. L. Nikonov
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Blood Serum ◽  
Clinical Guidelines ◽  
Reference Intervals ◽  
Healthy People ◽  
Helicobacter Pylori Infection ◽  
Pepsinogen I ◽  
H Pylori ◽  
Pepsinogen Ii

The purpose of this work was to familiarize doctors with the methods and significance of serological and cultural diagnostics of H. pylori infection on the example of the test for diagnosing the state of the gastric mucosa «Gastropanel». Blood serum tests were performed for 1057 patients and 122 healthy people aged 18-64 years: pepsinogen I (PG I), pepsinogen II (PG II), gastrin-17 (G-17), basal/stimulated), antibodies (IgGHp) to H. pylori (Biohit Oyj, Finland). The medians of the studied group indicators did not exceed the reference intervals. 398 (34%) patients have negative H. pylori status (IgGHp-). 275 (26%) patients with serum PG I≤70 mcg/ml were identified. The ratio of PG I/II≤3 in 84 (8%), 36 of them (43% of the group PG I/II≤3) - IgGHp-.

EXPERIENCE OF USING THE TEST SYSTEM «GASTROPANEL» IN THE PREVENTIVE MEDICAL EXAMINATION OF WORKFORCE IN JSC RZD

2021 ◽  
Author(s):  
A.O. Devyatko ◽  
◽  
T.A. Noskova ◽  
N.V. Shevchenko
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Enzyme Immunoassay ◽  
Test System ◽  
Medical Examination ◽  
Pepsinogen I ◽  
Non Invasive ◽  
H Pylori ◽  
Pepsinogen Ii

Abstract: Non-invasive diagnosis of the gastric mucosa was carried out in 181 people in the preventive medical examination of workforce in JSC RZD. A set of diagnostics from Vector-Best (Novosibirsk, Russia) was used for enzyme immunoassay of pepsinogen I, pepsinogen II, and immunoglobulins for Helicobacter pylori. The prevalence of H. pylori infection in more than half of the employees, the presence of deviations of biomarkers from the norm in more than one in three employees were revealed. Laboratory signs of atrophy were detected eight times less frequently than signs of inflammation of the gastric mucosa, but when infected with H. pylori and increased with age.

Sem Observations in Gastric Mucosa Exposed to Progressive Enzymatic Etching

1980 ◽  
Vol 38 ◽  
pp. 570-571
Author(s):  
C.A.E. Lemmi ◽  
D. Booth ◽  
G.E. Adomian
Keyword(s):  
Gastric Mucosa ◽  
Critical Point ◽  
Proteolytic Activity ◽  
Etching Process ◽  
Cellular Types

In order to enrich populations of homogeneous cellular types we dissociated gastric mucosa by enzymatic techniques. In addition, we used SEM to monitor the progressive etching of the mucosa. Two enzymes were tested: collagenase III with minimum proteolytic activity and Pronase with broader proteolytic effects. The gastric mucosa was exposed to the effect of the enzymes using everted stomach preparations. In this way the digestive action occured progressively from the lumen of the stomach toward the base of the glands. This “etching” process could be monitored conveniently by SEM. After incubation for periods varying from 30 to 210 minutes the tissues were stretched on dental wax, fixed in 2 % glutaralheyde, post-fixed in osmium, dehydrated, critical point dryed and coated with gold. A model MSM-5 “Mini-SEM” was used for observation. Gentle uncurling of the preparation before coating with gold produced fractures which revealed the structure of the gastric glandsin more detail.

SOLVOLYSABLE DEOXYCORTICOSTERONE CONJUGATES IN HUMAN URINE

1976 ◽  
Vol 68 (2) ◽  
pp. 273-281 ◽  
Author(s):  
C. L. COPE ◽  
S. LOIZOU
Keyword(s):  
Human Urine ◽  
Room Temperature ◽  
Urine Samples ◽  
Variable Ratio ◽  
The Mean ◽  
Sulphate Conjugate ◽  
Urine Specimens

SUMMARY The nature of the urinary conjugate converted by solvolysis, to free unconjugated deoxycorticosterone (DOC) was studied. A comparison of 11 solvolysis techniques has shown that the method employed in this study yielded 86% of the highest yield by any of the techniques tried. Three successive chromatographic systems on paper showed that no appreciable amounts of contaminants were present in the free DOC eluates, following solvolysis. By preparing authentic [3H]DOC sulphate and subjecting it to solvolysis it was shown that more than 90% of the tritiated DOC was recovered, after chromatography of the free DOC extract. This suggests that much of the solvolysable DOC in human urine is present in the form of the sulphate conjugate. The levels of DOC, excreted as the solvolysable conjugate in a variety of urine specimens, were shown to be much higher than those of free DOC, the former being 4·8 to 127 times higher than the amount of the latter. This highly variable ratio suggests that the site of production of solvolysable DOC is different from that for free DOC. The only correlation between free and solvolysable DOC was shown in dexamethasone-suppressed patients, in whom the mean percentage remaining after suppression was 30·6% for free DOC, 24·1% for solvolysable DOC and 22·2% for cortisol. As solvolysable DOC is present in much larger amounts in urine, care is necessary in the storage of urine samples in which free DOC estimates are to be made, as we found that urine specimens left at room temperature for 1 week could show rises of as much as 400% of their starting free DOC levels.

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