Skeletal muscle lactate dehydrogenase isozyme alterations in men and women marathon runners

1986 ◽  
Vol 61 (2) ◽  
pp. 477-481 ◽  
Author(s):  
F. S. Apple ◽  
M. A. Rogers
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Fiber Type ◽  
Muscle Lactate ◽  
Marathon Runners ◽  
Men And Women ◽  
Type Composition ◽  
Before And After ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme

Total lactate dehydrogenase (LD) and LD isozyme activities in gastrocnemius muscle from trained men and women runners were measured in response to the chronic stress of training for a marathon race (42.2 km). Following 9 wk of training, total LD activity in skeletal muscle from men and women runners significantly (P less than 0.02) decreased 2.26 and 2.25 U/mg protein, respectively. However, men's total LD activities were significantly (P less than 0.001) less than the women's both before and after training. Significant (P less than 0.05) increases in LD1 activities in skeletal muscle in men and women runners were also observed after training. No significant correlations were detected between percent fiber type composition in men or women vs. the changes in total LD activity, changes in LD1 activity, maximal O2 consumption or training distance averaged per week after the training period. The biochemical adaptations in skeletal muscle that occurred in the LD isozyme composition in both men and women runners make the runners skeletal muscle appear similar to heart muscle in LD1 and LD2 activities.

scholarly journals THE DISTRIBUTION OF LACTATE DEHYDROGENASE ISOZYMES IN HUMAN SKELETAL MUSCLE FIBERS

1964 ◽  
Vol 12 (8) ◽  
pp. 608-614 ◽  
Author(s):  
M. VAN WIJHE ◽  
M. C. BLANCHAER ◽  
S. ST. GEORGE-STUBBS
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Human Skeletal Muscle ◽  
Muscle Fibers ◽  
Isozyme Pattern ◽  
Electrophoretic Separation ◽  
Lactate Dehydrogenase Isozymes ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme ◽  
Normal Human

A study of the distribution of lactate dehydrogenase isozymes in single fibers from normal human skeletal muscle is presented. The fibers were classified into red, intermediate and white types on histochemical grounds and their lactate dehydrogenase isozyme content assessed by electrophoretic separation in veronal buffered agar. The results generally agreed with previous homogenate studies on animal skeletal muscle, in that the white fibers contained almost exclusively isozymes IV and V, whereas red fibers were rich in isozymes I, II and III, but IV and V also appeared indigenous to these fibers. The intermediate fibers had an isozyme pattern combining the features of red and white fibers. The metabolic implications of these findings are discussed.

Kinetics of lactate dehydrogenase from heart and white muscle of ocean pout: a single isozyme system

1986 ◽  
Vol 64 (12) ◽  
pp. 2665-2668 ◽  
Author(s):  
John M. Stewart ◽  
William R. Driedzic
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Kinetic Properties ◽  
Lactate Oxidase ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme ◽  
Kinetics Of ◽  
Insight Into ◽  
Dominant Direction

Heart and white skeletal muscle of ocean pout (Macrozoarces americanus) express only a single lactate dehydrogenase isozyme based on electrophoretic and immunological analysis. The enzyme has been partially purified and its kinetic properties elucidated in both the pyruvate reductase and lactate oxidase directions. Km values and responses to changing pH catagorize the enzyme as a classical skeletal muscle type lactate dehydrogenase. The kinetic parameters are assessed with respect to known in situ carbon flux rates through this locus. It is concluded that the enzyme data per se provide little insight into either the dominant direction or the net maximal rate of carbon flow.

Strength, power, fiber types, and mRNA expression in trained men and women with different ACTN3 R577X genotypes

2009 ◽  
Vol 106 (3) ◽  
pp. 959-965 ◽  
Author(s):  
Barbara Norman ◽  
Mona Esbjörnsson ◽  
Håkan Rundqvist ◽  
Ted Österlund ◽  
Ferdinand von Walden ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Fiber Type ◽  
Muscle Fiber Types ◽  
Vastus Lateralis Muscle ◽  
Fiber Types ◽  
Mrna Levels ◽  
Men And Women ◽  
Fiber Type Composition ◽  
Type Composition

α-Actinins are structural proteins of the Z-line. Human skeletal muscle expresses two α-actinin isoforms, α-actinin-2 and α-actinin-3, encoded by their respective genes ACTN2 and ACTN3. ACTN2 is expressed in all muscle fiber types, while only type II fibers, and particularly the type IIb fibers, express ACTN3. ACTN3 (R577X) polymorphism results in loss of α-actinin-3 and has been suggested to influence skeletal muscle function. The X allele is less common in elite sprint and power athletes than in the general population and has been suggested to be detrimental for performance requiring high power. The present study investigated the association of ACTN3 genotype with muscle power during 30-s Wingate cycling in 120 moderately to well-trained men and women and with knee extensor strength and fatigability in a subset of 21 men performing isokinetic exercise. Muscle biopsies were obtained from the vastus lateralis muscle to determine fiber-type composition and ACTN2 and ACTN3 mRNA levels. Peak and mean power and the torque-velocity relationship and fatigability output showed no difference across ACTN3 genotypes. Thus this study suggests that R577X polymorphism in ACTN3 is not associated with differences in power output, fatigability, or force-velocity characteristics in moderately trained individuals. However, repeated exercise bouts prompted an increase in peak torque in RR but not in XX genotypes, suggesting that ACTN3 genotype may modulate responsiveness to training. Our data further suggest that α-actinins do not play a significant role in determining muscle fiber-type composition. Finally, we show that ACTN2 expression is affected by the content of α-actinin-3, which implies that α-actinin-2 may compensate for the lack of α-actinin-3 and hence counteract the phenotypic consequences of the deficiency.

Skeletal muscle adaptations during early phase of heavy-resistance training in men and women

1994 ◽  
Vol 76 (3) ◽  
pp. 1247-1255 ◽  
Author(s):  
R. S. Staron ◽  
D. L. Karapondo ◽  
W. J. Kraemer ◽  
A. C. Fry ◽  
S. E. Gordon ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Resistance Training ◽  
Fiber Type ◽  
Dynamic Strength ◽  
Men And Women ◽  
Leg Extension ◽  
Type Composition ◽  
Muscle Adaptations ◽  
Skeletal Muscle Adaptations ◽  
Maximal Dynamic Strength

An 8-wk progressive resistance training program for the lower extremity was performed twice a week to investigate the time course for skeletal muscle adaptations in men and women. Maximal dynamic strength was tested biweekly. Muscle biopsies were extracted at the beginning and every 2 wk of the study from resistance-trained and from nontrained (control) subjects. The muscle samples were analyzed for fiber type composition, cross-sectional area, and myosin heavy chain content. In addition, fasting blood samples were measured for resting serum levels of testosterone, cortisol, and growth hormone. With the exception of the leg press for women (after 2 wk of training) and leg extension for men (after 6 wk of training), absolute and relative maximal dynamic strength was significantly increased after 4 wk of training for all three exercises (squat, leg press, and leg extension) in both sexes. Resistance training also caused a significant decrease in the percentage of type IIb fibers after 2 wk in women and 4 wk in men, an increase in the resting levels of serum testosterone after 4 wk in men, and a decrease in cortisol after 6 wk in men. No significant changes occurred over time for any of the other measured parameters for either sex. These data suggest that skeletal muscle adaptations that may contribute to strength gains of the lower extremity are similar for men and women during the early phase of resistance training and, with the exception of changes in the fast fiber type composition, that they occur gradually.

scholarly journals DEVELOPMENTAL CHANGE AND REGIONAL DIFFERENCE OF THE LACTATE DEHYDROGENASE ISOZYME PATTERN IN RAT BRAIN

1980 ◽  
Vol 13 (2) ◽  
pp. 164-172
Author(s):  
KANKATSU YUN ◽  
TAKESHI MATSUO ◽  
TAKASHI ORIBE ◽  
TSUTOMU TOMIOKA ◽  
TAKAYOSHI IKEDA
Keyword(s):  
Lactate Dehydrogenase ◽  
Rat Brain ◽  
Regional Difference ◽  
Developmental Change ◽  
Isozyme Pattern ◽  
Dehydrogenase Isozyme ◽  
Lactate Dehydrogenase Isozyme
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