A chamber for the perfusion of in vitro tissue with multiple solutions

There are currently no practical systems that allow extended regions (>5 mm2) of a tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to pharmacological manipulation. Previous work has only achieved this at the expense of access to the tissue for recording electrodes. Here, we present a chamber that allows a tissue slice to be maintained in multiple solutions, at physiological temperatures, and preserves the ability to record from the slice. We demonstrate the effectiveness of the tissue bath with respect to minimizing the mixing of the solutions, maintaining the viability of the tissue, and preserving the ability to record from the slice simultaneously.

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In Vitro Electrochemical Properties of Titanium Nitride Neural Stimulating and Recording Electrodes as a Function of Film Thickness and Voltage Biasing

10.1109/embc46164.2021.9630715 ◽

2021 ◽

Author(s):

Justin R. Abbott ◽

Alexandra Joshi-Imre ◽

Stuart F. Cogan

Keyword(s):

Film Thickness ◽

Titanium Nitride ◽

Electrochemical Properties ◽

Recording Electrodes

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Impedance characterization of microarray recording electrodes in vitro

The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society ◽

10.1109/iembs.2004.1404210 ◽

2005 ◽

Cited By ~ 1

Author(s):

D.R. Merrill ◽

P.A. Tresco

Keyword(s):

Impedance Characterization ◽

Recording Electrodes

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Optimized polyethylenimine (PEI)-based nanoparticles for siRNA delivery, analyzed in vitro and in an ex vivo tumor tissue slice culture model

Drug Delivery and Translational Research ◽

10.1007/s13346-016-0306-y ◽

2016 ◽

Vol 7 (2) ◽

pp. 206-216 ◽

Cited By ~ 15

Author(s):

Alexander Ewe ◽

Sabrina Höbel ◽

Claudia Heine ◽

Lea Merz ◽

Sonja Kallendrusch ◽

...

Keyword(s):

Tumor Tissue ◽

Ex Vivo ◽

Sirna Delivery ◽

Tissue Slice ◽

Slice Culture ◽

Culture Model

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Constructing a Computer Model of the Human Eye Based on Tissue Slice Images

International Journal of Biomedical Imaging ◽

10.1155/2010/921469 ◽

2010 ◽

Vol 2010 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Peishan Dai ◽

Boliang Wang ◽

Chunbo Bao ◽

Ying Ju

Keyword(s):

Computer Model ◽

Geometric Model ◽

Three Dimensional ◽

Tissue Slice ◽

Central Axis ◽

Virtual Surgery ◽

Three Dimensional Model ◽

Human Eye ◽

Eye Tissues

Computer simulation of the biomechanical and biological heat transfer in ophthalmology greatly relies on having a reliable computer model of the human eye. This paper proposes a novel method on the construction of a geometric model of the human eye based on tissue slice images. Slice images were obtained from an in vitro Chinese human eye through an embryo specimen processing methods. A level set algorithm was used to extract contour points of eye tissues while a principle component analysis was used to detect the central axis of the image. The two-dimensional contour was rotated around the central axis to obtain a three-dimensional model of the human eye. Refined geometric models of the cornea, sclera, iris, lens, vitreous, and other eye tissues were then constructed with their position and ratio relationships kept intact. A preliminary study of eye tissue deformation in eye virtual surgery was simulated by a mass-spring model based on the computer models developed.

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271 Pharmacological manipulation of Alzheimer's amyloid β protein in vitro and in vivo

Neurobiology of Aging ◽

10.1016/s0197-4580(96)80273-9 ◽

1996 ◽

Vol 17 (4) ◽

pp. S68

Author(s):

C.B. Eckman ◽

C.-M. Prada ◽

A. Fauq ◽

S.G. Younkin

Keyword(s):

Amyloid Β ◽

Amyloid Β Protein ◽

Pharmacological Manipulation ◽

Β Protein

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Effect of Prolonged Cold Exposure on in Vitro Respiration and Anaerobic Glycolysis of Rat Liver

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1958.192.2.253 ◽

1958 ◽

Vol 192 (2) ◽

pp. 253-257 ◽

Cited By ~ 9

Author(s):

John P. Hannon

Keyword(s):

Respiratory Rate ◽

Rat Liver ◽

Cold Exposure ◽

Tissue Slice ◽

Exact Nature ◽

Anaerobic Glycolysis ◽

Control Value ◽

Liver Slices

Liver slices were prepared from rats that had been exposed to cold (5 ± 1°C) for intervals of 2, 4, 6 or 9 weeks, and assays were made for respiratory rate and the rate of anaerobic glycolysis. The results of the respiration experiments indicated the first 4 weeks' cold exposure was associated with increasing qo2 values, whereas exposure longer than 4 weeks was associated with qo2 that returned toward the control value. The results indicated further that the exact nature of the substances being metabolized in the conventional tissue slice experiment was unknown. The results of the anaerobic experiments indicated the rate of anaerobic glycolysis decreased progressively as the duration of cold exposure was increased.

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Humoral deficiency and reticuloendothelial depression after traumatic shock

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1976.230.1.7 ◽

1976 ◽

Vol 230 (1) ◽

pp. 7-14 ◽

Cited By ~ 71

Author(s):

JE Kaplan ◽

TM Saba

Keyword(s):

Bone Marrow ◽

Phagocytic Activity ◽

Direct Relationship ◽

Traumatic Shock ◽

Tissue Slice ◽

Opsonic Activity ◽

Progressive Decline ◽

Clearance Kinetics

Circulating opsonin levels and reticuloendothelial (RE) phagocytic activity were investigated in anesthetized rats subjected to Noble-Collip drum (NCD) trauma. Reticuloendothelial function was assessed by colloid clearance kinetics and circulating opsonin levels by in vitro tissue slice bioassay. After sublethal shock, both hepatic RE phagocytosis and plasma opsonic activity were significantly (P less than 0.001) depressed in the 0.5- to 6-h posttrauma period. Pulmonary and bone marrow localization of the blood-borne test microparticles significantly (P less than 0.05) increased during hepatic RES depression. Hepatic RE cells from animals during the interval of posttraumatic in vivo phagocytic depression exhibited normal phagocytosis when incubated in normal pretrauma plasma and decreased phagocytic activity when incubated in posttrauma plasma. After sublethal shock, restoration or opsonin levels by 24 h after shock resulted in hepatic RE recovery. Plasma opsonin levels declined in direct relationship to the degree of trauma. Progressive hepatic RE failure was correlated with the progressive decline in circulating plasma opsonic activity. The findings indicate that opsonic depletion may be involved in the etiology of hepatic reticuloendothelial depression after traumatic shock.

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Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study

Neuroscience Research ◽

10.1016/j.neures.2016.02.006 ◽

2016 ◽

Vol 109 ◽

pp. 16-27 ◽

Cited By ~ 7

Author(s):

Matthias Wolff ◽

Patrick Czorlich ◽

Chandran Nagaraj ◽

Rose Schnöbel-Ehehalt ◽

Yingji Li ◽

...

Keyword(s):

Ion Channel ◽

Dorsal Horn ◽

In Vitro Study ◽

Tissue Slice ◽

Vitro Study ◽

Voltage Gated ◽

Dorsal Horn Neurons

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Factors Influencing the Histochemical Demonstration of Coenzyme-Dependent Dehydrogenases and Diaphorases

The Journal of Cell Biology ◽

10.1083/jcb.5.2.309 ◽

1959 ◽

Vol 5 (2) ◽

pp. 309-318 ◽

Cited By ~ 16

Author(s):

Joseph Cascarano ◽

Benjamin W. Zweifach

Keyword(s):

Alcohol Dehydrogenase ◽

Histochemical Demonstration ◽

Tissue Slice ◽

In Vitro Studies ◽

Tetrazolium Chloride ◽

Tetrazolium Salts ◽

Patterns Of Distribution ◽

Factors Influencing ◽

Different Levels

Procedures for the histochemical demonstration of DPN and TPN diaphorases have been presented by other workers. These techniques rely on the coenzyme-dependent dehydrogenases present in the tissue slice to generate the substrate required by the diaphorases. In vitro studies were carried out on kidney and adrenal tissue of the rat, using NT (neotetrazolium) and INT (2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride) with various substrates of DPN-dependent dehydrogenases. The solutions used for study contained alcohol and alcohol dehydrogenase, glutamate and malate, malate, glutamate, ß-hydroxybutyrate, or DPNH. It has been possible to demonstrate (1) that histological distribution of dehydrogenases may differ from that of the flavoprotein oxidizing reduced coenzyme I; (2) characteristic patterns of distribution of particular dehydrogenases in the tissue proper; (3) different levels of dehydrogenase in kidney and adrenal; and (4) differences in dehydrogenase distribution in the kidneys of man and rat. The evidence presented clearly indicates the limitations inherent in the accepted procedures for the demonstration of DPN and TPN diaphorases. The possible application of the tetrazolium salts to the study of particular coenzyme-dependent dehydrogenases and the pitfalls which might occur are also discussed.

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