scholarly journals DNA Binding and Photocleavage Studies of Cobalt(III) Ethylenediamine Pyridine Complexes: [Co(en)2(py)2]3+ and [Co(en)2(mepy)2]3+

2008 ◽  
Vol 2008 ◽  
pp. 1-8 ◽  
Author(s):  
Penumaka Nagababu ◽  
D. Aravind Kumar ◽  
Kotha Laxma Reddy ◽  
K. Ashwini Kumar ◽  
Md. B. Mustafa ◽  
...  
Keyword(s):  
Dna Binding ◽  
Anticancer Activity ◽  
Plasmid Dna ◽  
Emission Spectroscopy ◽  
Calf Thymus Dna ◽  
Dna Melting ◽  
Viscosity Measurements ◽  
Pyridine Complexes ◽  
Calf Thymus ◽  
Ct Dna

Two novel cobalt(III) pyridine complexes (1)[Co(en)2(py)2]3+ and (2)[Co(en)2(mepy)2]3+ (en=ethylenediamine, py=pyridine, and mepy=methylpyridine) have been synthesized and characterized. The interaction of these complexes with calf thymus DNA was investigated by absorption, emission spectroscopy, viscosity measurements, DNA melting, and DNA photocleavage. Results suggest that the two complexes bind to DNA via groove mode and complex 2 binds more strongly to CT DNA than complex 1. Moreover, these Co(III) complexes have been found to promote the photocleavage of plasmid DNA pBR322 under irradiation at 365 nm, cytotoxicity results of complexes are also showing anticancer activity.

scholarly journals DNA Binding and Photocleavage Studies of Cobalt(III) Polypyridine Complexes: [Co(en)2PIP]3+, [Co(en)2IP]3+, and [Co(en)2phen-dione]3+

2007 ◽  
Vol 2007 ◽  
pp. 1-8 ◽  
Author(s):  
Penumaka Nagababu ◽  
S. Satyanarayana
Keyword(s):  
Plasmid Dna ◽  
Emission Spectroscopy ◽  
Spectroscopic Studies ◽  
Calf Thymus Dna ◽  
Dna Melting ◽  
Base Pairs ◽  
Viscosity Measurements ◽  
Calf Thymus ◽  
Polypyridine Complexes ◽  
Ct Dna

In this paper, three complexes of type[Co(en)2PIP]3+(PIP=2-phenylimidazo[4,5-f][1,10,] phenanthroline)(1),[Co(en)2IP]3+(IP=imidazo[4,5-f][1,10,] phenanthroline)(2), and[Co(en)2phen-dione]3+(1,10 phenanthroline 5,6,dione)(3) have been synthesized and characterized by UV/VIS, IR,1HNMR spectral methods. Absorption spectroscopy, emission spectroscopy, viscosity measurements, and DNA melting techniques have been used for investigating the binding of these two complexes with calf thymus DNA, and photocleavage studies were used for investigating these binding of these complexes with plasmid DNA. The spectroscopic studies together with viscosity measurements and DNA melting studies support that complexes 1 and 2 bind to CT DNA(=calf thymus DNA) by intercalation mode via IP or PIP into the base pairs of DNA, and complex 3 is binding as groove mode. Complex 1 binds more avidly to CT DNA than 2 and 3 which is consistent with the extended planar ringπsystem of PIP. Noticeably, the two complexes have been found to be efficient photosensitisers for strand scissions in plasmid DNA.

Synthesis, structure, and calf-thymus DNA binding of ternary fleroxacin–Cu(ii) complexes

RSC Advances ◽  
2016 ◽  
Vol 6 (83) ◽  
pp. 80286-80295 ◽  
Author(s):  
Ying Xiao ◽  
Kailin Xu ◽  
Qing Wang ◽  
Xinnuo Xiong ◽  
Yanmei Huang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Calf Thymus Dna ◽  
Calf Thymus ◽  
Ct Dna

The interaction between the synthesized fleroxacin complexes and CT DNA was investigated.

scholarly journals Comprehensive exploration of the anticancer activities of procaine and its binding with calf thymus DNA: a multi spectroscopic and molecular modelling study

RSC Advances ◽  
2018 ◽  
Vol 8 (17) ◽  
pp. 9083-9093 ◽  
Author(s):  
Mohd. Sajid Ali ◽  
Mohammad Abul Farah ◽  
Hamad A. Al-Lohedan ◽  
Khalid Mashay Al-Anazi
Keyword(s):  
Anticancer Activity ◽  
Molecular Modelling ◽  
Minor Groove ◽  
Calf Thymus Dna ◽  
Anticancer Activities ◽  
Demethylating Agent ◽  
Calf Thymus ◽  
Ct Dna ◽  
Modelling Study

DNA demethylating agent procaine binds at the minor groove of ct-DNA and increases the anticancer activity of doxorubicin.

scholarly journals Synthesis, DNA-binding and antiproliferative activity of N-(Nitrogen heterocyclic) norcantharidin acylamide acid

2009 ◽  
Vol 7 (3) ◽  
pp. 569-575 ◽  
Author(s):  
Wen-Zhong Zhu ◽  
Rui-Ding Hu ◽  
Qiu-Yue Lin ◽  
Xiao-Xia Wang ◽  
Xiao-Liang Zheng
Keyword(s):  
Dna Binding ◽  
Cell Line ◽  
Elemental Analysis ◽  
Antiproliferative Activity ◽  
Plasmid Dna ◽  
H Nmr ◽  
Smmc7721 Cell ◽  
Calf Thymus ◽  
Binding Experiment ◽  
Pbr322 Plasmid

AbstractTwo novel norcantharidin acylamide acids (HL1=N-pyrimidine norcantharidin acylamide acid, C12H13N3O4; HL2=N-pyridine norcantharidin acylamide acid, C13H14N2O4) were synthesized by a reaction of norcantharidin(NCTD) with 2-aminopyrimidine and 2-aminopyridine, respectively. Their structures were characterized by elemental analysis, IR, UV and 1 H NMR. Fluorescence titration and viscosity measurements indicated that HL1, HL2 and HL3 (HL3=N-phenyl norcantharidin acylamide acid, C14H15NO4) can bind calf thymus DNA via partial intercalation. The liner Stern-Volmer quenching constant Ksv values for HL1, HL2 and HL3 were 2.05 × 104 L mol−1, 1.15 × 104 L mol−1 and 8.30×103 L mol−1, respectively. Two compounds containing heterocycle of HL1 and HL2 have been found to cleave pBR322 plasmid DNA at physiological pH and temperature. The test of antiproliferation activity showed that the compounds had moderate to strong antiproliferative ability against the tested cell lines except of HL3 against the SMMC7721 cell line. The results indicated that the heterocycle attached to the norcantharidin was favorable to antiproliferative activity. This result was consistent with the DNA binding experiment.

Binding of an anionic fluorescent probe with calf thymus DNA and effect of salt on the probe–DNA binding: a spectroscopic and molecular docking investigation

RSC Advances ◽  
2014 ◽  
Vol 4 (108) ◽  
pp. 63549-63558 ◽  
Author(s):  
Saptarshi Ghosh ◽  
Pronab Kundu ◽  
Bijan Kumar Paul ◽  
Nitin Chattopadhyay
Keyword(s):  
Molecular Docking ◽  
Dna Binding ◽  
Fluorescent Probe ◽  
Binding Mode ◽  
Docking Studies ◽  
Calf Thymus Dna ◽  
Molecular Docking Studies ◽  
Biologically Relevant ◽  
Calf Thymus

Binding mode of biologically relevant anionic probe, ANS, with ctDNA is divulged from spectroscopic and molecular docking studies.

Synthesis, biological investigation, calf thymus DNA binding and docking studies of the sulfonyl hydrazides and their derivatives

2016 ◽  
Vol 1107 ◽  
pp. 99-108 ◽  
Author(s):  
Shahzad Murtaza ◽  
Saima Shamim ◽  
Naghmana Kousar ◽  
Muhammad Nawaz Tahir ◽  
Muhammad Sirajuddin ◽  
...  
Keyword(s):  
Dna Binding ◽  
Docking Studies ◽  
Calf Thymus Dna ◽  
Biological Investigation ◽  
Calf Thymus

DNA-binding, photocleavage and anti-cancer activity of tin(IV) corrole

2018 ◽  
Vol 22 (09n10) ◽  
pp. 739-750 ◽  
Author(s):  
An-Na Xie ◽  
Zhao Zhang ◽  
Hua-Hua Wang ◽  
Atif Ali ◽  
Dong-Xu Zhang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Mode ◽  
Calf Thymus Dna ◽  
Free Base ◽  
Docking Analysis ◽  
Calf Thymus ◽  
Anti Cancer ◽  
Active Intermediate ◽  
Dna Scission ◽  
Cancer Activity

A new tin(IV) corrole, 5,10,15-tris(4-methoxycarbonylphenyl) corrole tin(IV) (1-Sn) was synthesized and characterized. The DNA binding, photocleavage and anti-cancer activity were studied and compared with its free-base. The interaction of 1-Sn and its free-base 1 with calf thymus DNA had been investigated by spectroscopic methods, viscosity measurements and molecular docking analysis. The results revealed that 1-Sn and 1 could interact with calf thymus DNA via an outside groove binding mode. Furthermore, although 1 displayed no photonuclease activity, 1-Sn exhibited good photonuclease activity as indicated by agarose gel electrophoresis, and superoxide anion might be the active intermediate for the DNA scission. Finally, 1 was nontoxic but 1-Sn displayed cytotoxicity towards A549 tumor cell lines.

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