scholarly journals Cells in Dengue Virus Infection In Vivo

2010 ◽  
Vol 2010 ◽  
pp. 1-15 ◽  
Author(s):  
Sansanee Noisakran ◽  
Nattawat Onlamoon ◽  
Pucharee Songprakhon ◽  
Hui-Mien Hsiao ◽  
Kulkanya Chokephaibulkit ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Emerging Infectious Diseases ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Severe Dengue ◽  
Dengue Virus Infection ◽  
Dengue Disease

Dengue has been recognized as one of the most important vector-borne emerging infectious diseases globally. Though dengue normally causes a self-limiting infection, some patients may develop a life-threatening illness, dengue hemorrhagic fever (DHF)/dengue shock syndrome (DSS). The reason why DHF/DSS occurs in certain individuals is unclear. Studies in the endemic regions suggest that the preexisting antibodies are a risk factor for DHF/DSS. Viremia and thrombocytopenia are the key clinical features of dengue virus infection in patients. The amounts of virus circulating in patients are highly correlated with severe dengue disease, DHF/DSS. Also, the disturbance, mainly a transient depression, of hematological cells is a critical clinical finding in acute dengue patients. However, the cells responsible for the dengue viremia are unresolved in spite of the intensive efforts been made. Dengue virus appears to replicate and proliferate in many adapted cell lines, but these in vitro properties are extremely difficult to be reproduced in primary cells or in vivo. This paper summarizes reports on the permissive cells in vitro and in vivo and suggests a hematological cell lineage for dengue virus infection in vivo, with the hope that a new focus will shed light on further understanding of the complexities of dengue disease.

scholarly journals Roles for Endothelial Cells in Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nadine A. Dalrymple ◽  
Erich R. Mackow
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Capillary Permeability ◽  
Virus Disease ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Barrier Functions ◽  
Dengue Disease ◽  
Primary Fluid

Dengue viruses cause two severe diseases that alter vascular fluid barrier functions, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The endothelium is the primary fluid barrier of the vasculature and ultimately the effects of dengue virus infection that cause capillary leakage impact endothelial cell (EC) barrier functions. The ability of dengue virus to infect the endothelium provides a direct means for dengue to alter capillary permeability, permit virus replication, and induce responses that recruit immune cells to the endothelium. Recent studies focused on dengue virus infection of primary ECs have demonstrated that ECs are efficiently infected, rapidly produce viral progeny, and elicit immune enhancing cytokine responses that may contribute to pathogenesis. Furthermore, infected ECs have also been implicated in enhancing viremia and immunopathogenesis within murine dengue disease models. Thus dengue-infected ECs have the potential to directly contribute to immune enhancement, capillary permeability, viremia, and immune targeting of the endothelium. These effects implicate responses of the infected endothelium in dengue pathogenesis and rationalize therapeutic targeting of the endothelium and EC responses as a means of reducing the severity of dengue virus disease.

scholarly journals Manifestation of thrombocytopenia in dengue-2-virus-infected mice

2000 ◽  
Vol 81 (9) ◽  
pp. 2177-2182 ◽  
Author(s):  
Kao-Jean Huang ◽  
Shu-Yi J. Li ◽  
Shiour-Ching Chen ◽  
Hsiao-Sheng Liu ◽  
Yee-Shin Lin ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Clinical Manifestations ◽  
Dengue Shock Syndrome ◽  
Dengue Virus Infection ◽  
Platelet Antibody

Dengue virus infection causes dengue fever, dengue haemorrhagic fever and dengue shock syndrome. No animal model is available that mimics these clinical manifestations. In this study, the establishment is reported of a murine model for dengue virus infection that resembles the thrombocytopenia manifestation. Dengue-2 virus (dengue virus type 2) can infect murine cells either in vitro (primary cell culture) or in vivo. Viraemia detected by RT–PCR was found transiently at 2 days after intravenous injection of dengue-2 virus. Transient thrombocytopenia developed at 10–13 days after primary or secondary infection. Anti-platelet antibody was generated after dengue-2 virus infection. There was strain variation in dengue-2 virus infection; the A/J strain was more sensitive than BALB/c or B6 mice. This dengue-2-virus-infected mouse system accompanied by thrombocytopenia and anti-platelet antibody will be a valuable model to study the pathogenicity of dengue virus infection.

scholarly journals UPDATE MANAGEMENT OF DENGUE COMPLICATION IN PEDIATRIC

2015 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Soegeng Soegijanto
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Performance ◽  
Kidney Injury ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Liver Involvement ◽  
Dengue Virus Infection ◽  
Important Health ◽  
Grade Iii

Dengue virus infection is one of the important health problems in Indonesia, although the mortality rate has been decreased but many dengue shock syndrome cases is very difficult to be solving handled. It might be due to nature course of dengue virus infection is very difficult to predict of the earlier time of severity occur. THE AIM To get idea to make update management of dengue complication in pediatric. MATERIAL AND METHOD Data were compiled from Dr. Soetomo Hospital Surabaya in 2009. The diagnosis of all cases was based on criteria WHO 1997 and PCR examination in Institute Tropical Disease for identified serotype of dengue virus infection. The unusual cases of dengue virus infection were treated following the new WHO protocol in 2009. RESULT There were only 3 cases with serotype DEN 1, consisted 2 cases had age 1–4 years and 1 had age 5–14 years. 2 cases showed a severe clinical performance as dengue shock syndrome and 1 case showed as unusual case of dengue virus infection. Three report cases of: a. Dengue hemorrhagic fever grade III which liver involvement and had bilateral pleural effusion; b. Dengue hemorrhagic grade III with liver involvement and encephalopathy; c. Dengue hemorrhagic grade III with liver involvement acute kidney injury, myocardial involvement and encephalopathy. All the patients were treated according to new edition WHO protocol and all of the involving organ recovered along with the improvement of the disease. CONCLUSION Update management of dengue complication pediatric should be learned carefully used for helping unusual cases of dengue virus infection.

scholarly journals Protective Capacity of the Human Anamnestic Antibody Response during Acute Dengue Virus Infection

2016 ◽  
Vol 90 (24) ◽  
pp. 11122-11131 ◽  
Author(s):  
Meihui Xu ◽  
Roland Züst ◽  
Ying Xiu Toh ◽  
Jennifer M. Pfaff ◽  
Kristen M. Kahle ◽  
...  
Keyword(s):  
Virus Infection ◽  
Antibody Response ◽  
Dengue Virus ◽  
Secondary Infection ◽  
Immune Memory ◽  
Protective Capacity ◽  
Dengue Virus Infection ◽  
Immune Correlates

ABSTRACT Half of the world's population is exposed to the risk of dengue virus infection. Although a vaccine for dengue virus is now available in a few countries, its reported overall efficacy of about 60% is not ideal. Protective immune correlates following natural dengue virus infection remain undefined, which makes it difficult to predict the efficacy of new vaccines. In this study, we address the protective capacity of dengue virus-specific antibodies that are produced by plasmablasts a few days after natural secondary infection. Among a panel of 18 dengue virus-reactive human monoclonal antibodies, four groups of antibodies were identified based on their binding properties. While antibodies targeting the fusion loop of the glycoprotein of dengue virus dominated the antibody response, two smaller groups of antibodies bound to previously undescribed epitopes in domain II of the E protein. The latter, largely serotype-cross-reactive antibodies, demonstrated increased stability of binding at pH 5. These antibodies possessed weak to moderate neutralization capacity in vitro but were the most efficacious in promoting the survival of infected mice. Our data suggest that the cross-reactive anamnestic antibody response has a protective capacity despite moderate neutralization in vitro and a moderate decrease of viremia in vivo . IMPORTANCE Antibodies can protect from symptomatic dengue virus infection. However, it is not easy to assess which classes of antibodies provide protection because in vitro assays are not always predictive of in vivo protection. During a repeat infection, dengue virus-specific immune memory cells are reactivated and large amounts of antibodies are produced. By studying antibodies cloned from patients with heterologous secondary infection, we tested the protective value of the serotype-cross-reactive “recall” or “anamnestic” response. We found that results from in vitro neutralization assays did not always correlate with the ability of the antibodies to reduce viremia in a mouse model. In addition, a decrease of viremia in mice did not necessarily improve survival. The most protective antibodies were stable at pH 5, suggesting that antibody binding in the endosomes, after the antibody-virus complex is internalized, might be important to block virus spread in the organism.

scholarly journals Upaya Pencegahan dan Pengendalian Demam Berdarah Dengue dalam Bentuk Peta Tematik di Kecamatan Rappocini

bionature ◽  
2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Suci Wulandhani ◽  
A. Bida Purnamasari ◽  
Ryan Humardani Syam Pratomo
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Hemorrhagic Fever ◽  
Viral Disease ◽  
High Rate ◽  
Dengue Virus Infection ◽  
Dengue Disease ◽  
Keywords Prevention ◽  
Short Time ◽  
And Control

Abstract. Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus infection. One symptom of dengue virus infection is high fever and headache. Dengue virus is a virus from the genus Flavivirus, family of Flaviviridae. This dengue fever is a dangerous viral disease because it can cause sufferers to die in a very short time / several days. The existence and population density are often associated with transmission, endemicity and Extraordinary Events (EE) of DHF. Disease mapping by utilizing digital technology to support epidemiological investigations and also as a tool to monitor regional conditions for dengue disease. The purpose of this study was to determine efforts to prevent and control dengue disease by mapping. The results of the study show that mapping can be seen as increasing and decreasing the number of cases of dengue disease so that it can assist in data management and reporting of information to monitor areas at risk of contracting dengue disease. Suggestions given need to do further research using other variables that are the cause of the high rate of dengue cases, so that it can be utilized by related agencies as the basis of the information system supporting the decision on preventive measures to combat dengue. Keywords: prevention and control, DHF, mapping.

scholarly journals In vitro and in vivo roles of sphingosine kinase 2 during dengue virus infection

2019 ◽  
Vol 100 (4) ◽  
pp. 629-641 ◽  
Author(s):  
Wisam H. Al-Shujairi ◽  
Jennifer N. Clarke ◽  
Lorena T. Davies ◽  
Melissa R. Pitman ◽  
Julie K. Calvert ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Sphingosine Kinase ◽  
Dengue Virus Infection ◽  
Sphingosine Kinase 2

scholarly journals Monoclonal antibody-mediated enhancement of dengue virus infection in vitro and in vivo and strategies for prevention

2007 ◽  
Vol 104 (22) ◽  
pp. 9422-9427 ◽  
Author(s):  
A. P. Goncalvez ◽  
R. E. Engle ◽  
M. St. Claire ◽  
R. H. Purcell ◽  
C.-J. Lai
Keyword(s):  
Monoclonal Antibody ◽  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Virus Infection

scholarly journals Serotype and Clinical Performance of Dengue Virus Infection on the Year 2009

2010 ◽  
Vol 1 (2) ◽  
pp. 55 ◽  
Author(s):  
Soegeng Soegijanto ◽  
Widodo Darmowandowo ◽  
Amor Peraten Ginting ◽  
Atsushi Yamanaka
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestation ◽  
Clinical Performance ◽  
Secondary Infection ◽  
Severe Disease ◽  
Dengue Shock Syndrome ◽  
Severe Dengue ◽  
Rt Pcr ◽  
Dengue Virus Infection

Dengue hemorrhagic fever is one of the important health problem in Indonesia, mortality rate is becoming decrease but many dengue shock syndrome cases is very difficult to be help. Previous study showed that some of DEN 2 and DEN 3 virus cases could show a clinical performance of severe dengue virus infection such as dengue shock syndrome. There are four serotype of dengue virus infection can cause primary and secondary infection. The aim of this research is to know the relationship between clinical performance of dengue virus infection and serotype dengue virus and also to know the role of primary and secondary infection and age of dengue virus cases. A prospective analytic observational study, which was conducted in Dr. Soetomo hospital since January 2009. RT-PCR was used to attempt to identify the infecting serotype from dengue virus isolated using vero cell. Antibody responses were measured by ELISA and clinical manifestation were measured with the WHO criteria 1997. Dengue serotype identification by RT-PCR was 70 patients. Virus types were DEN-2 65(92.8%), DEN-1 3(4.2%), and DEN-3 2(2.8%). Patients with DEN-1 genotype IV were more trend severe disease DSS and unusual infection. Commanly usually secondary exposure cause more severe clinical manifestation than primary exposure (p = 0.035) but in this study found that all of DEN-1 genotype IV, primary or secondary infection to show severe clinical manifestation of dengue virus infection. We can conclude that DEN-2 was the most dominant serotype in Dr. Soetomo Hospital. On Primary and secondary infection, DEN-1 genotype IV showing more severe than DEN-2 and DEN-3.

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