scholarly journals Development and Statistical Validation of Spectrophotometric Methods for the Estimation of Nabumetone in Tablet Dosage Form

2010 ◽  
Vol 7 (4) ◽  
pp. 1463-1467 ◽  
Author(s):  
A. R. Rote ◽  
S. R. Bhalerao
Keyword(s):  
Area Under Curve ◽  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Statistical Validation ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Derivative Spectra ◽  
Pharmaceutical Tablet ◽  
Tablet Dosage

Three new simple, economic spectrophotometric methods were developed and validated for the estimation of nabumetone in bulk and tablet dosage form. First method includes determination of nabumetone at absorption maxima 330 nm, second method applied was area under curve for analysis of nabumetone in the wavelength range of 326-334 nm and third method was First order derivative spectra with scaling factor 4. Beer law obeyed in the concentration range of 10-30 μg/mL for all three methods. The correlation coefficients were found to be 0.9997, 0.9998 and 0.9998 by absorption maxima, area under curve and first order derivative spectra. Results of analysis were validated statistically and by performing recovery studies. The mean percent recoveries were found satisfactory for all three methods. The developed methods were also compared statistically using one way ANOVA. The proposed methods have been successfully applied for the estimation of nabumetone in bulk and pharmaceutical tablet dosage form.

scholarly journals First-order derivative spectrophotometric estimation of gemifloxacin mesylate and ambroxol HCl in tablet dosage form

2021 ◽  
Vol 14 (2) ◽  
pp. 029-036
Author(s):  
Wrushali A. Panchale ◽  
Ravindra L. Bakal
Keyword(s):  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
First Order ◽  
Derivative Spectroscopy ◽  
Ich Guidelines ◽  
Tablet Dosage

Aim of present work was to develop and validate a simple, precise and accurate uv-vis spectrophotometric method for the simultaneous estimation of gemifloxacin mesylate (GEMI) and ambroxol HCl (AMB) in their combined tablet dosage form. The method is based on first-order derivative spectroscopy. For determination of sampling wavelengths, each of GEMI and AMB were scanned in the wavelength range of 200–400 nm in the spectrum mode and sampling wavelengths were selected at 360 nm (zero crossing of GEMI) where AMB showed considerable absorbance and at 221.6 nm (zero crossing of AMB) where GEMI showed considerable absorbance. The linearity was obtained in the concentration range of 32-192 µg/ml for GEMI and 7.5-45 µg/ml for AMB. The correlation coefficients were found to be 0.9987 and 0.9992 for GEMI and AMB, respectively. The method was validated as per ICH guidelines. Mean recoveries were found satisfactory. All the data of validation study was found to be satisfactorily. The proposed method can be applied for simultaneous estimation of both the drugs

scholarly journals Estimation of Levocetirizine in Bulk and Formulation by First Order Derivative Area under Curve UV-Spectrophotometric Methods.

2016 ◽  
Vol 2 (1) ◽  
pp. 09
Author(s):  
Pandurang Tukaram Mane
Keyword(s):  
Area Under Curve ◽  
Pharmaceutical Formulations ◽  
Order Derivative ◽  
Pharmaceutical Dosage Forms ◽  
Mean Values ◽  
First Order ◽  
Spectrophotometric Methods ◽  
Ich Guidelines ◽  
Significant Difference

Simple, fast and reliable spectrophotometric methods were developed for determination of Levocetirizine in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 235-243 nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Levocetirizine using 5-25?g/ml (r=0.9994) for first order Derivative Area under Curve spectrophotometric method. The proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Levocetirizine in pharmaceutical formulations.

Simultaneous Estimation of Ofloxacin, Clotrimazole, and Lignocaine Hydrochloride in Their Combined Ear-Drop Formulation by Two Spectrophotometric Methods

2017 ◽  
Vol 100 (1) ◽  
pp. 38-44
Author(s):  
Kunjan Bodiwala ◽  
Shailesh Shah ◽  
Yogini Patel ◽  
Pintu Prajapati ◽  
Bhavin Marolia ◽  
...  
Keyword(s):  
Dosage Form ◽  
Correlation Coefficients ◽  
The Other ◽  
Simultaneous Estimation ◽  
Standard Mixture ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
Derivative Spectra ◽  
Derivative Method ◽  
Ear Drops

Abstract Two sensitive, accurate, and precise spectrophotometric methods have been developed and validated for the simultaneous estimation of ofloxacin (OFX), clotrimazole (CLZ), and lignocaine hydrochloride (LGN) in their combined dosage form (ear drops) without prior separation. The derivative ratio spectra method (method 1) includes the measurement of OFX and CLZ at zero-crossing points (ZCPs) of each other obtained from the ratio derivative spectra using standard LGN as a divisor, whereas the measurement of LGN at the ZCP of CLZ is obtained from the ratio derivative spectra using standard OFX as a divisor. The double divisor-ratio derivative method (method 2) includes the measurement of each drug at its amplitude in the double divisor-ratio spectra obtained using a standard mixture of the other two drugs as the divisor. Both methods were found to be linear (correlation coefficients of >0.996) over the ranges of 3–15, 10–50, and 20–100 μg/mL for OFX, CLZ, and LGN, respectively; precise (RSD of <2%); and accurate (recovery of >98%) for the estimation of each drug. The developed methods were successfully applied for the estimation of these drugs in a marketed ear-drop formulation. Excipients and other ingredients did not interfere with the estimation of these drugs. Both methods were statistically compared using the t-test.

scholarly journals Development and Validation of First-Order Derivative Spectrophotometry for Simultaneous Determination of Levocetirizine Dihydrochloride and Phenylephrine Hydrochloride in Pharmaceutical Dosage Form

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Kaminee Parmar ◽  
Sunil Baldania ◽  
Dimal Shah ◽  
Usmangani Chhalotiya ◽  
Naimin Parmar
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Zero Crossing ◽  
Phenylephrine Hydrochloride ◽  
First Order ◽  
Crossing Point

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of levocetirizine dihydrochloride (LCT) and phenylephrine hydrochloride (PHE) by employing first-order derivative spectrophotometric method. The first-order derivative absorption at 240 nm (zero crossing point of PHE) was used for quantification of LCT and 283.2 nm (zero crossing point of LCT) for quantification of PHE. The linearity was established over the concentration range of 4–24 μg/mL and 8–48 μg/mL for LCT and PHE with correlation coefficients (r2) 0.9964 and 0.9972, respectively. The mean % recoveries were found to be in the range of 99.14%–100.43% for LCT and 98.73%–100.83% for PHE. The proposed method has been validated as per ICH guideline and successfully applied for the simultaneous estimation of LCT and PHE in combined tablet dosage form.

scholarly journals UV Spectrophotometric Method for theEstimation of Itopride Hydrochloride in Pharmaceutical Formulation

2010 ◽  
Vol 7 (s1) ◽  
pp. S49-S54 ◽  
Author(s):  
K. R. Gupta ◽  
R. R. Joshi ◽  
R. B. Chawla ◽  
S. G. Wadodkar
Keyword(s):  
Wavelength Range ◽  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Pharmaceutical Formulations ◽  
Sharp Peak ◽  
Order Derivative ◽  
Distilled Water ◽  
First Order ◽  
Derivative Spectra ◽  
Itopride Hydrochloride

Three simple, precise and economical UV methods have been developed for the estimation of itopride hydrochloride in pharmaceutical formulations. Itopride hydrochloride in distilled water shows the maximum absorbance at 258.0 nm (Method A) and in first order derivative spectra of the same shows sharp peak at 247.0 nm, when n = 1 (Method B). Method C utilises area under curve (AUC) in the wavelength range from 262.0-254.0 nm for analysis of itopride hydrochloride. The drug was found to obey Beer-Lambert’s law in the concentration range of 5-50 μg/mL for all three proposed methods. Results of the analysis were validated statistically and recovery studies were found to be satisfactory.

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