scholarly journals First-order derivative spectrophotometric estimation of gemifloxacin mesylate and ambroxol HCl in tablet dosage form

2021 ◽  
Vol 14 (2) ◽  
pp. 029-036
Author(s):  
Wrushali A. Panchale ◽  
Ravindra L. Bakal
Keyword(s):  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
First Order ◽  
Derivative Spectroscopy ◽  
Ich Guidelines ◽  
Tablet Dosage

Aim of present work was to develop and validate a simple, precise and accurate uv-vis spectrophotometric method for the simultaneous estimation of gemifloxacin mesylate (GEMI) and ambroxol HCl (AMB) in their combined tablet dosage form. The method is based on first-order derivative spectroscopy. For determination of sampling wavelengths, each of GEMI and AMB were scanned in the wavelength range of 200–400 nm in the spectrum mode and sampling wavelengths were selected at 360 nm (zero crossing of GEMI) where AMB showed considerable absorbance and at 221.6 nm (zero crossing of AMB) where GEMI showed considerable absorbance. The linearity was obtained in the concentration range of 32-192 µg/ml for GEMI and 7.5-45 µg/ml for AMB. The correlation coefficients were found to be 0.9987 and 0.9992 for GEMI and AMB, respectively. The method was validated as per ICH guidelines. Mean recoveries were found satisfactory. All the data of validation study was found to be satisfactorily. The proposed method can be applied for simultaneous estimation of both the drugs

scholarly journals A NEW VALIDATED THIRD ORDER DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND RAMIPRIL IN TABLET DOSAGE FORM

2020 ◽  
pp. 54-59
Author(s):  
ALEKHYA B. ◽  
M. SINDHUSHA ◽  
SORAJ K. RAUL ◽  
GOPAL K. PADHY
Keyword(s):  
Quantitative Analysis ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Third Order ◽  
Metoprolol Succinate ◽  
Zero Crossing ◽  
Ich Guidelines ◽  
Tablet Dosage

Objective: The objective of the present work is to develop and validate a new UV derivative spectrophotometric method for simultaneous estimation of metoprolol succinate and ramipril in methanol: water (50:50v/v). Methods: “Zero crossing technique” was chosen for quantitative determination. The zero-crossing points (ZCP’s) were found to be 209 nm where metoprolol succinate was quantified and 211 nm where ramipril was quantified. This method was then subjected to accuracy, linearity, sensitivity and reproducibility according to ICH guidelines to ensure and confirm its validity. Results: The method was found to be obeying Beer’s law in the range of 10-50 µg/ml and 5-25 µg/ml for metoprolol succinate and ramipril, respectively. The % recoveries were observed between the range of 99.2-100.2 for metoprolol succinate and 99.57-99.86 for ramipril. The intra-day and inter-day results showed reproducibility. Conclusion: It can be concluded that the developed third-order UV derivative spectroscopic method for the simultaneous determination of metoprolol succinate and ramiprilcan be recommended for routine quantitative analysis.

scholarly journals Development and Validation of First-Order Derivative Spectrophotometry for Simultaneous Determination of Levocetirizine Dihydrochloride and Phenylephrine Hydrochloride in Pharmaceutical Dosage Form

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Kaminee Parmar ◽  
Sunil Baldania ◽  
Dimal Shah ◽  
Usmangani Chhalotiya ◽  
Naimin Parmar
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Zero Crossing ◽  
Phenylephrine Hydrochloride ◽  
First Order ◽  
Crossing Point

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of levocetirizine dihydrochloride (LCT) and phenylephrine hydrochloride (PHE) by employing first-order derivative spectrophotometric method. The first-order derivative absorption at 240 nm (zero crossing point of PHE) was used for quantification of LCT and 283.2 nm (zero crossing point of LCT) for quantification of PHE. The linearity was established over the concentration range of 4–24 μg/mL and 8–48 μg/mL for LCT and PHE with correlation coefficients (r2) 0.9964 and 0.9972, respectively. The mean % recoveries were found to be in the range of 99.14%–100.43% for LCT and 98.73%–100.83% for PHE. The proposed method has been validated as per ICH guideline and successfully applied for the simultaneous estimation of LCT and PHE in combined tablet dosage form.

SIMULTANEOUS ESTIMATION OF EBASTINE AND MONTELUKAST SODIUM IN TABLET DOSAGE FORM BY UV-SPECTROPHOTOMETRY AND FIRST ORDER DERIVATIVE

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (10) ◽  
pp. 47-52
Author(s):  
T. N Patil ◽  
◽  
S. D Firke ◽  
S. B Bari ◽  
N. S Joshi ◽  
...  
Keyword(s):  
Dosage Form ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Cross Point ◽  
Zero Crossing ◽  
First Order ◽  
Montelukast Sodium ◽  
The Mean ◽  
Tablet Dosage

A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of ebastine and montelukast sodium by employing first order derivative zero crossing method in methanol and water. The first order derivative absorption at 234 nm (zero cross point of ebastine) was used for quantification of montelukast sodium and 281.60 nm (zero cross point of montelukast sodium) for quantification of ebastine. The linearity was established over the concentration range of 5-25 μg/mL for both ebastine and montelukast sodium with correlation coefficient r2 0.997 and 0.998, respectively. The mean % recoveries were found to be in the range of 102.8 – 103.2 % and 101 - 105 % for ebastine and montelukast sodium, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of ebastine and montelukast sodium in their combined tablet dosage form.

Development and Validation of Derivative Spectroscopic Method for Simultaneous Estimation of Cefadroxil and Probenecid

2014 ◽  
Vol 7 (1) ◽  
pp. 2350-2355
Author(s):  
Pratik S Mehta ◽  
Pratik R. Patel ◽  
Rajesh R Parmar ◽  
M M K Modasiya ◽  
Dushyant A Shah
Keyword(s):  
Spectroscopic Method ◽  
Spectral Interference ◽  
Synthetic Mixture ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
First Order ◽  
Derivative Spectroscopy ◽  
Development And Validation ◽  
Crossing Point

A novel, simple, accurate, sensitive, precise and economical derivative spectroscopic method was developed and validated for the determination of cefadroxil and probenecid in synthetic mixture. First order derivative spectroscopy method was adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges of 4-36 μg/ml for cefadroxil and of 5-25 μg/ml of probenecid. The zero crossing point for cefadroxil and probenecid was 260 nm and 237.8 nm respectively in 0.1N HCl. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to synthetic mixture and no interference from the synthetic mixture’s excipients was found.   

scholarly journals A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PYRANTEL PAMOATE AND PRAZIQUANTEL IN BULK AND PHARMACEUTICAL DOSAGE FORM

2019 ◽  
pp. 62-67 ◽  
Author(s):  
Rajesh R. ◽  
JITHU JERIN JAMES
Keyword(s):  
High Performance ◽  
Dosage Form ◽  
Correlation Coefficients ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Pyrantel Pamoate ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Tablet Dosage

Objective: To develop a simple, accurate and precise reverse-phase high-performance liquid chromatography (RP-HPLC) method and subsequently validate for the simultaneous estimation of praziquantel (PZQ) and pyrantel pamoate (PP) in the pharmaceutical dosage form. Methods: The chromatographic separation was achieved on Phenomenex Luna C18 column (250 mm × 4.6 mm, 5 μm) as stationary phase maintained at an ambient temperature with a mobile phase comprising of water: acetonitrile (20: 80) at a flow rate of 1.0 ml/min and UV detection at 220 nm. Results: The retention time of PZQ and PP was found to be 3.897 min and 1.697 min respectively. The method was validated in terms of specificity, accuracy, precision, linearity and robustness as per ICH guidelines. Linearity was obtained in the concentration range of 20–60 μg/ml for both PZQ and PP with correlation coefficients of 0.987 and 0.998 respectively. The accuracy of the method was determined using a recovery test and found as 98.44 % to 100.35 %. All parameters are found to be within the acceptable limit. Conclusion: The developed RP-HPLC method was simple, rapid, accurate, precise for the simultaneous estimation of PZQ and PP in bulk and tablet dosage form.

Novel First Order Derivative UV Spectrophotometric Method for the Determination of Glimepiride in Solid Dosage Forms

2018 ◽  
Vol 8 (3) ◽  
Author(s):  
Santosh Karajgi . ◽  
Sunayana Mali ◽  
Ramaling Kotnal
Keyword(s):  
Dosage Forms ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Drug Form ◽  
Solid Dosage Forms ◽  
First Order ◽  
Solid Dosage ◽  
Tablet Dosage ◽  
Guide Lines

Objective: An easy, perfect, specific and exact process has been studied for the simultaneous estimation of Glimepiride pure drug form as well as tablet dosage forms. Methods: A UV method for quantitative evaluation of Glimepiride by first order derivative peak detect method for determination in bulk as well as tablet dosage form is reported as there was a need to expand novel methods to analyze the drug. Results: Glimepiride has absorbance first derivative maxima at 225 nm in Methanol. Glimepiride follows Beer’s law in concentration range of 5-25µg/ml. The outcomes of the study were validated statistically and recovery studies were performed as per ICH guide lines. Conclusion: Thus the projected method can be applied competently for the estimation of Glimepiride in regular analysis in its dosage forms.

scholarly journals Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
S. Venkatesan ◽  
N. Kannappan
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tablet Dosage

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation, and sensitive-free direct UV spectrophotometric method is developed and validated for the simultaneous estimation of some antiviral drugs such as emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), and rilpivirine HCl (RPV) in tablet dosage form by Vierordt’s method. The solutions of standard and sample were prepared in methanol. The λmax⁡ for emtricitabine, tenofovir disoproxil fumarate, and rilpivirine hydrochloride were 240.8 nm, 257.6 nm, and 305.6 nm, respectively. Calibration curves are linear in the concentration ranges 4–12 μg/ml for EMT, 6–18 μg/ml for TDF, and 0.5–1.5 μg/ml for RPV, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.

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