In VitroPropagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components

Handroanthus impetiginosus, pink lapacho, is a timber, ornamental, and medicinal tree. Experiments on thein vitropropagation ofH. impetiginosuswere conducted using nodal segments cultivated in both Murashige and Skoog salts with Gamborg vitamins (MSG) and Woody Plant Medium (WPM) with different concentrations of 6-benzylaminopurine (BA) and indole butyric acid (IBA). Morphogenic responses were differentially affected by salt compositions and their interactions with plant growth regulators in each micropropagation stage. According to response surface analysis, the optimum multiplication rate with 1 μM IBA ranged from 16.7 to 21.3 μM BA in WPM, and the inhibitors of endogenous auxins could increase multiplication rates. A pulse with 50 μM IBA in1/2MSG produced 83% rooting with 3.2 roots per shoots and higher fresh and dry weights of shoots and roots. In the acclimatisation stage, 50% of plants survived after 1 year. This methodology optimised the culture media for thein vitropropagation of theH. impetiginosusclonal pool and could be applied to related species, several of which are categorised as vulnerable on the International Union for the Conservation of Nature Red List.

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588 PB 129 IN VITRO MULTIPLICATION OF STEWARTIA MALACODENDRON L., AN ENDANGERED WOODY SPECIES

HortScience ◽

10.21273/hortsci.29.5.516b ◽

1994 ◽

Vol 29 (5) ◽

pp. 516b-516

Author(s):

Murdock Ray Gillis ◽

Michael E. Kane

Keyword(s):

Woody Plant ◽

Unit Cost ◽

Woody Species ◽

Shoot Multiplication ◽

Shoot Tips ◽

Nodal Segments ◽

Deciduous Tree ◽

Landscape Plant ◽

Woody Plant Medium

Stewartia malacodendron L. (silky camellia), a small deciduous tree bearing showy flowers, has potential as a landscape plant. Propagation problems, limited availability and consequent high unit cost have slowed its acceptance as a landscape plant. Given its potential value as a landscape plant, studies were initiated to define a micropropagation protocol. Surface sterilized shoot tips and nodal explants from two-year-old container grown seedlings were established on Woody Plant Medium supplemented with 4.44 μM benzyladenine (BA) and solidified with 0.8% TC® Agar. Sustained growth of subcultured shoot tips and nodal segments required the addition of 8-15 μM gibberellic acid (GA3) to the medium. Regenerated shoots were 3 - 5 cm long, unbranched and typically consisted of three subdivisions. Effects of cytokinin type (BA, 2iP or kinetin) and concentration (0-25 μM) with factorial combinations of GA3 (0-30 μM) on shoot multiplication, elongation and diameter were determined after a 28 day culture period. Moderate GA, levels (10 & 20 μM) in combination with 2.5 μM BA yielded the highest quality microcuttings.

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100 A Comparison of Two Media on Growth of Deciduous Azalea Shoots In Vitro

HortScience ◽

10.21273/hortsci.34.3.458e ◽

1999 ◽

Vol 34 (3) ◽

pp. 458E-458

Author(s):

Carol D. Robacker ◽

Betty Robicheaux

Keyword(s):

Woody Plant ◽

Culture Media ◽

Shoot Tips ◽

Fresh Medium ◽

First Flush ◽

Wide Range ◽

Woody Plant Medium ◽

Made In

Micropropagation is a useful technique to propagate species such as deciduous azaleas, which are difficult to root from cuttings. To develop a micropropagation protocol that would be effective with a wide range of species and cultivars of native azalea, two culture media, Woody Plant Medium (WPM) (Lloyd and McCown, 1980) and ER medium (Economou and Read, 1994) were evaluated for ability to support growth of 11 species and four cultivars of deciduous azalea. Shoot tips were obtained from the first flush of growth in the spring on plants growing in the greenhouse or field. Following disinfection, the terminal and basal ends were removed from each explant. The explants were placed in culture tubes containing either WPM or ER medium with 12 mg/L 2iP and solidified with agar. Cultures were transferred to fresh medium every 4 to 6 weeks. Initial evaluations were made in 1996, and the experiment was repeated in 1997. In 1998, six of the taxa were evaluated for a third year. For most of the taxa evaluated, growth was superior on ER medium. On WPM, many of the cultures browned and died. R. canescens, R. viscosum, R. prunifolium, and R. austrinum are examples of species that preferred ER medium. R. alabamense, R. arborescens, and `My Mary' performed similarly on either medium.

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In Vitro Propagation of Oriental White Oak Quercus aliena Blume

Forests ◽

10.3390/f10060463 ◽

2019 ◽

Vol 10 (6) ◽

pp. 463

Author(s):

Qiansheng Li ◽

Mengmeng Gu ◽

Min Deng

Keyword(s):

Woody Plant ◽

Germplasm Conservation ◽

Shoot Tips ◽

Nodal Segments ◽

Adventitious Bud ◽

White Oak ◽

Widespread Species ◽

Aseptic Culture ◽

Woody Plant Medium

Quercus aliena Blume, also known as the oriental white oak, is a widespread species in temperate forests of East Asia with significant ecological and economical importance. Establishing an efficient vegetative propagation system is important for its germplasm conservation and breeding program. Protocols of micropropagation from shoot tips and nodal segments were investigated in order to produce uniform high-quality seedlings. Nodal segments from 18 month old seedlings were used as explants to initiate the aseptic culture. The highest bud proliferation was achieved by subculturing the explants on 1/2 strength woody plant medium (WPM) with 2.0 mg·L−1 BA. WPM with 0.5 mg·L−1 BA and 0.05 mg·L−1 IBA was the best medium for subculture to obtain the vigorous regenerated shoots in this experiment. Nodal segments without shoot tips had a higher adventitious bud proliferation rate than those with shoot tips. The highest rate (41.5%) of rooting in vitro was induced by using WPM with 1.0 mg·L−1 IBA and 5 g·L−1 activated charcoal. Ex vitro rooting by dipping the proliferated shoots with 500 mg·L−1 IBA solution, then transplanting directly to potting mix with 50% peat and 50% horticultural perlite fostered the highest rooting percentage and survival rate of the plantlets.

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Propagación in vitro de Psidium guajaba mediante organogénesis directa a partir de segmentos nodales

Ciencia y Tecnología Agropecuaria ◽

10.21930/rcta.vol8_num1_art:79 ◽

2007 ◽

Vol 8 (1) ◽

pp. 22 ◽

Cited By ~ 1

Author(s):

Fabiola Ocampo ◽

Víctor Manuel Núñez

Keyword(s):

In Vitro Propagation ◽

Woody Plant ◽

Adventitious Shoots ◽

Nodal Segments ◽

Direct Organogenesis ◽

Post Inoculation ◽

Ex Vitro ◽

Mass Multiplication ◽

Woody Plant Medium

Se indujeron múltiples brotes mediante organogénesis directa a partir de segmentos nodales de 10 genotipos diferentes de guayaba. Para ello se estableció un sistema de propagación clonal in vitro combinado con inducción rápida de brotes ex vitro para propagar árboles élite. La utilización de segmentos nodales permitió obtener en poco tiempo brotes adventicios adecuados para multiplicación masiva. La respuesta in vitro de los genotipos fue evaluada usando los medios de cultivo MS (Murashige y Skoog, 1962), Mc (Mascarenhas) y WPM (Woody Plant Medium) suplementados con 0,1 mg•L-1 de ácido indolácetico (AIA) y 0,25 mg•L-1 de bencilaminopurina (BAP). El procedimiento de desinfección con hipoclorito de sodio previno eficientemente la contaminación de los explantes después de la inoculación en el medio de cultivo. El mayor porcentaje en la inducción de brotes se logró con 0,25 mg•L-1 de BAP. Los segmentos nodales presentaron de 1 a 2 brotes adventicios por explante después de 15 días de inoculados y de 3 a 7 brotes a los 30 días después del inicio del cultivo. Una vez individualizados los brotes se usaron en una nueva fase de multiplicación masiva en la que se probaron cuatro sustratos diferentes durante el enraizamiento y el endurecimiento. Esta metodología permitió la propagación in vitro de guayaba cuatro semanas después del inicio del cultivo. Los mejores resultados se lograron con el medio WPM que permitió obtener las primeras plántulas enraizadas dos semanas después de la transferencia al sustrato de enraizamiento. In vitro propagation of Psidium guajaba using direct organogenesis from nodal segmentsMultiple shoots were induced using direct organogenesis from nodal segments of 10 different genotypes of guayaba. For this an in vitro clonal propagation system combined with rapid ex vitro induction of shoots was established in order to propagate elite trees. The use of nodal segments resulted in adventitious shoots adequate for mass multiplication in less time. The in vitro response of the genotypes was evaluated using the culture media MS (Murashige and Skoog, 1962), Mc (Mascarenhas) and WPM (Woody Plant Medium) supplemented with 0.1 mg·L-1 of indole acetic acid (IAA) and 0.25 mg·L-1 of benzoaminopurine (BAP). The procedure for sterilizing with sodium hypochlorite effectively prevented the contamination of the explants after the inoculation of the culture medium. The greatest percentage of shoot induction was achieved with 0.25 mg·L-1 of BAP. The nodal segments showed between 1-2 adventitious shoots per explant 15 days post-inoculation and 3-7 shoots 30 days post-inoculation. Once individualized, the shoots were used in a new mass multiplication phase in which four different substrates were tested during rooting and hardening. This methodology permitted the in vitro propagation of guayaba four weeks post-inoculation. The best results were achieved with the WPM medium that resulted in the first rooted plantlets two weeks after the transfer to the rooting substrate.

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In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants

Agricultura ◽

10.1515/agricultura-2017-0008 ◽

2016 ◽

Vol 13 (1-2) ◽

pp. 65-69

Author(s):

Metka Šiško

Keyword(s):

Survival Rate ◽

Woody Plant ◽

Contamination Rate ◽

Multiplication Rate ◽

Culture Initiation ◽

Hydrangea Macrophylla ◽

Genotype 2 ◽

Woody Plant Medium ◽

Dichloroisocyanuric Acid

Abstract The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA.

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In Vitro and Macropropagation of the Wildflower Dyssodia pentacheta (D. C.) Robins

HortScience ◽

10.21273/hortsci.26.12.1555 ◽

1991 ◽

Vol 26 (12) ◽

pp. 1555-1557

Author(s):

Thomas W. Zimmerman ◽

Fred T. Davies ◽

Jayne M. Zajicek

Keyword(s):

Shoot Growth ◽

Woody Plant ◽

Shoot Proliferation ◽

Nodal Segments ◽

Naphthaleneacetic Acid ◽

Stem Cuttings ◽

Nodal Explant ◽

System A ◽

Woody Plant Medium

Dyssodia pentacheta, a prostrate-growing perennial Texas wildflower with potential for use in low-maintenance landscapes, was propagated in vitro and by stem cuttings under mist. Optimum rooting for IBA-treated semihardwood terminal stem cuttings (3 to 30 mm IBA) and in vitro-grown nodal segments (30 to 100 mm IBA) occurred after 4 weeks under an intermittent mist system. A 300-mm IBA basal dip was lethal to macroand microcuttings. In vitro, D. pentacheta produced more shoots per nodal explant on Woody Plant Medium (2 g Gelrite/liter) with 1 to 10 μ m BA than with combinations of BA and 0.5 μm NAA. After shoot proliferation, the shoots were subculture twice and grown on growth regulator-free medium. When maintaining D. pentacheta in vitro on media devoid of plant growth regulators, 1% sucrose was more effective than 2% for promoting shoot growth and suppressing apparent production of phenolics. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA); 1H-indole-3-butyric acid (IBA); 1-naphthaleneacetic acid (NAA).

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In Vitro Propagation of HS314 Rootstock (Prunus amygdalus × P. persica)

HortScience ◽

10.21273/hortsci.46.6.928 ◽

2011 ◽

Vol 46 (6) ◽

pp. 928-931

Author(s):

Jalil Dejampour ◽

Islam Majidi ◽

Solmaz Khosravi ◽

Sevil Farhadi ◽

Atena Shadmehr

Keyword(s):

Culture Media ◽

Shoot Proliferation ◽

Nodal Segments ◽

Naphthaleneacetic Acid ◽

Root Induction ◽

Prunus Amygdalus ◽

Indole Butyric Acid ◽

Half Strength

A micropropagation protocol was developed for the HS314 rootstock, a hybrid between almond and peach that could be used as an alternative rootstock instead of GF677. Surface-sterilized nodal segments were cultured in a modified DKW medium containing 3% sucrose, 100 mg·L−1 of Phloroglucinol, 0.7% plant agar, and 0.5 mg·L−1 benzyl amino purine (BAP). Explants were transferred to the same culture media supplemented with 0.5, 1, or 2 mg·L−1 BAP and 0, 0.1, or 0.5 mg·L−1 indole butyric acid (IBA) for further shoot proliferation. The maximum number of shoots produced on a medium containing 2 mg·L−1 BAP. Microshoots were transferred to the DKW medium supplemented with 0.5, 1, 2, or 4 mg·L−1 IBA or naphthaleneacetic acid (NAA) for root induction. The highest root number and the greatest root length were gained on a medium containing 2 mg·L−1 IBA. Rooting percentage was improved from 66% to more than 85% by reducing the concentration of DKW salts to half strength. Finally, rooted plantlets were successfully acclimatized and transferred in vivo conditions.

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Rosmanol controls explants browing of Hypericum canariensis L. during the in vitro estabilishment of shoots

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1997.042 ◽

2014 ◽

Vol 66 (3-4) ◽

pp. 347-349 ◽

Cited By ~ 2

Author(s):

Sebastiana Mederos ◽

Lucía San Andrés ◽

Javier G. Luis

Keyword(s):

Natural Products ◽

Canary Islands ◽

Efficient Method ◽

Woody Plant ◽

Culture Media ◽

Medicinal Species ◽

Woody Plant Medium

An efficient method for eradication of browning exudate was developed for Hypericum canariensis. For this purpose the effect of natural products on browning exudates were investigated in four types of culture media: Murashige and Skoog (MS, 1962); Gamborg's (B5, 1979); Woody Plant Medium (WPM, Lloyd and McCown 1981) and modified Quoirin and Lepoivre (QL.4) (Mederos 1991, Mederos et al. 1995) basal macroelements; these basal macroelements were supplemented with the microelement formula described by Murashige and Skoog (MS, 1962). During the establishment of shoots organogenesis potential was achieved in the Murashige and Skoog (MS, 1962) and modified Quoirin and Lepoivre (QL. 4) (Mederos 1991, Mederos et al. 1995) media after browning exudates was eliminated by rosmanol treatments. Rosmanol is a powerful diterpenic antioxidant isolated from Salvia canariensis L., a medicinal species endemic to the Canary Islands.

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Period of harvest, sprouting ability of cuttings, and in vitro plant regeneration in Fraxinus excelsior

Canadian Journal of Botany ◽

10.1139/b94-035 ◽

1994 ◽

Vol 72 (2) ◽

pp. 261-267 ◽

Cited By ~ 9

Author(s):

Conceição Eneida Silveira ◽

Alain Cottignies

Keyword(s):

Chromosome Number ◽

In Vitro Culture ◽

Adventitious Roots ◽

Woody Plant ◽

Fraxinus Excelsior ◽

Stem Cuttings ◽

Natural Conditions ◽

Apical Bud ◽

Woody Plant Medium

Propagation by stem cuttings and in vitro culture of apical bud explants were studied on Fraxinus excelsior L. Stem cuttings from 4- to 7-year-old trees growing under natural conditions sprouted only when cuttings were taken from dormant material. Only 6% of those that had sprouted developed roots by the 7th month of culture. Similarly, only apical bud explants harvested during the dormant period sprouted in vitro. Up to 87% of these sprouts developed two to four branching adventitious roots after 5 months of culture. During the initial phase of in vitro culture, the Quoirin and Lepoivre medium and the woody plant medium favoured sprout lengthening. During the phase of multiplication, up to three sprouts per explant developed with the woody plant medium in the presence of a combination of high 6-benzylaminopurine (3.0–4.0 mg∙L−1) and low indole-3-butyric acid (0.01–0.03 mg∙L−1) concentrations. Rooting was obtained in a medium without any growth regulators. Microscopic analysis showed a direct connection between the vascular elements of adventitious roots and stem of plantlet. Chromosome number in root apices of ash plantlets and ash trees grown under natural conditions was 2n = 46. Key words: chromosome number, Fraxinus excelsior L., in vitro plants, micropropagation, stem cuttings.

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In vitro establishment and proliferation of red currant cultivars

Horticultural Science ◽

10.17221/40/2011-hortsci ◽

2012 ◽

Vol 39 (No. 1) ◽

pp. 21-25 ◽

Cited By ~ 2

Author(s):

J. Sedlák ◽

F. Paprštein

Keyword(s):

Woody Plant ◽

Adventitious Bud ◽

Mineral Salts ◽

The Czech Republic ◽

Shoot Number ◽

Adventitious Bud Formation ◽

Woody Plant Medium ◽

In Vitro Establishment ◽

Red Currant

The goal of this study was to investigate in vitro multiplication protocols for use with red currant cultivars grown in the Czech Republic. Cultivars Detvan, Vitan and Rotte Höllandische were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 25.7%. Two proliferation media Murashige and Skoog medium (MS) and McCown woody plant medium (WPM) containing 1 or 2 mg/l of 6-benzylaminopurine (BAP) were tested. Initial explants produced new plants in the form of rosettes. Rosettes arose from the base of the initial explants in the form of adventitious bud formation. The shoot number was relatively low and varied between 1.0 and 2.1. Generally, the highest number was obtained for cultivar Rotte Holländische that produced 2.1 ± 0.1 new rosettes on MS medium containing lower concentration 1 mg/l BAP. In contrary, Vitan cv. had significantly lower shoot number ranging from 1.0 to 1.3. WPM medium with a lower concentration of mineral salts proved to be unsuitable for the multiplication of tested cultivars.

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