scholarly journals In vitro tissue culture initiation from potted and garden Hydrangea macrophylla explants

Agricultura ◽  
2016 ◽  
Vol 13 (1-2) ◽  
pp. 65-69
Author(s):  
Metka Šiško
Keyword(s):  
Survival Rate ◽  
Woody Plant ◽  
Contamination Rate ◽  
Multiplication Rate ◽  
Culture Initiation ◽  
Hydrangea Macrophylla ◽  
Genotype 2 ◽  
Woody Plant Medium ◽  
Dichloroisocyanuric Acid

Abstract The effect of sterilisation treatments on the initiation of the culture of three Hydrangea macrophylla genotypes in vitro was studied. The results indicated difference among different disinfection treatment; the consequence of treatments with dichloroisocyanuric acid, in combination with silver nitrate, was a significantly higher survival rate comparing to treatments based on NaOCl. Success of sterilisation was significantly influenced by studied genotypes. Two potted hydrangea genotypes (G2 and G3) showed higher survival rate (45.00 %, 55.00 %, respectively) comparing to the genotype 1 (originated from a local garden), which exhibited the highest percentage of contamination rate (58.75 %). Culturing the explants on a McCown woody plant medium supplemented with BAP at 2 mg/L and NAA at 0.005 mg/L produced the highest number of shoots with multiplication rate of 1.31 in 5 weeks. The highest rooting percentage (100.00 %) was obtained with the genotype 2 on a McCown woody plant medium supplemented with 0.5 mg/L IBA.

scholarly journals Use of Forcing Solution Techniques to Improve Chestnut Micropropagation

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 757A-757 ◽  
Author(s):  
Guochen Yang ◽  
Paul E. Read ◽  
Marihelen Kamp-Glass
Keyword(s):  
Woody Plant ◽  
Shoot Proliferation ◽  
Contamination Rate ◽  
Regenerate Shoot ◽  
Normal Morphology ◽  
Woody Plant Medium ◽  
Forcing Solution ◽  
High Contamination Rate ◽  
Solution Techniques

Chestnut (Castanea spp.) is considered difficult to micropropagate. The timing for harvesting explant materials from forced stems is critical, although many factors need to be considered for successful micropropagation. Previous research with spirea and five-leaf aralia demonstrated that forcing solution techniques extended the availability of high-quality explant material, thus expediting micropropagation. However, preliminary research illustrated that chestnut is very difficult to force and the new forced softwood growth is very short-lived, which made micropropagation difficult. It was found that, at about 7 days from budbreak, the forced chestnut softwood growth (about 2 cm long) served as the best explant material. If longer than this timing window, the new growth would die. If shorter, the explants had a high contamination rate, exudation of purported phenolic compounds, and explants would not regenerate. Shoot proliferation and callus regeneration were achieved by culturing good-quality explants on Woody Plant Medium supplemented with 0.1 mg BA/liter. The new shoots grew vigorously in vitro with apparent normal morphology.

scholarly journals 052 In Vitro Culture Initiation and Proliferation of Exochorda racemosa

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 397C-397
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
In Vitro Culture ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Deionized Water ◽  
In Vitro Cultures ◽  
Culture Initiation ◽  
Woody Plant Medium ◽  
Ornamental Shrub ◽  
Ornamental Species

Exochorda racemosa is an ornamental shrub with white flowers that is spiraea-like, deciduous, and hardy. The buds resemble pearls. Normally it is propagated by seeds, layers, and cuttings of softwood. However, it is a slow process that takes a few years to produce a reasonable size plant for the demanding market. Our objective was to establish a successful in vitro culture and to rapidly multiply this ornamental species. Softwood explant materials were collected from a local nursery and were disinfested with 15% bleach solution and rinsed three times with sterile distilled and deionized water. In vitro cultures were established and maintained in woody plant medium (WPM) supplemented with BA at 0.1 mg·L-1, 3% sucrose, and 0.7% agar with the pH adjusted to 5.8. Then shoots were transferred to the multiplication medium containing BA, CPPU, or thidiazuron (TDZ) at various concentrations. Preliminary results show that explants cultured on medium containing TDZ produced the best shoot proliferation.

scholarly journals In VitroPropagation of Pink Lapacho: Response Surface Methodology and Factorial Analysis for Optimisation of Medium Components

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Ezequiel Enrique Larraburu ◽  
Nancy Mariel Apóstolo ◽  
Berta Elizabet Llorente
Keyword(s):  
Response Surface ◽  
Woody Plant ◽  
Culture Media ◽  
Nodal Segments ◽  
Multiplication Rate ◽  
Indole Butyric Acid ◽  
Medicinal Tree ◽  
Medium Components ◽  
Woody Plant Medium

Handroanthus impetiginosus, pink lapacho, is a timber, ornamental, and medicinal tree. Experiments on thein vitropropagation ofH. impetiginosuswere conducted using nodal segments cultivated in both Murashige and Skoog salts with Gamborg vitamins (MSG) and Woody Plant Medium (WPM) with different concentrations of 6-benzylaminopurine (BA) and indole butyric acid (IBA). Morphogenic responses were differentially affected by salt compositions and their interactions with plant growth regulators in each micropropagation stage. According to response surface analysis, the optimum multiplication rate with 1 μM IBA ranged from 16.7 to 21.3 μM BA in WPM, and the inhibitors of endogenous auxins could increase multiplication rates. A pulse with 50 μM IBA in1/2MSG produced 83% rooting with 3.2 roots per shoots and higher fresh and dry weights of shoots and roots. In the acclimatisation stage, 50% of plants survived after 1 year. This methodology optimised the culture media for thein vitropropagation of theH. impetiginosusclonal pool and could be applied to related species, several of which are categorised as vulnerable on the International Union for the Conservation of Nature Red List.

Period of harvest, sprouting ability of cuttings, and in vitro plant regeneration in Fraxinus excelsior

1994 ◽  
Vol 72 (2) ◽  
pp. 261-267 ◽  
Author(s):  
Conceição Eneida Silveira ◽  
Alain Cottignies
Keyword(s):  
Chromosome Number ◽  
In Vitro Culture ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Fraxinus Excelsior ◽  
Stem Cuttings ◽  
Natural Conditions ◽  
Apical Bud ◽  
Woody Plant Medium

Propagation by stem cuttings and in vitro culture of apical bud explants were studied on Fraxinus excelsior L. Stem cuttings from 4- to 7-year-old trees growing under natural conditions sprouted only when cuttings were taken from dormant material. Only 6% of those that had sprouted developed roots by the 7th month of culture. Similarly, only apical bud explants harvested during the dormant period sprouted in vitro. Up to 87% of these sprouts developed two to four branching adventitious roots after 5 months of culture. During the initial phase of in vitro culture, the Quoirin and Lepoivre medium and the woody plant medium favoured sprout lengthening. During the phase of multiplication, up to three sprouts per explant developed with the woody plant medium in the presence of a combination of high 6-benzylaminopurine (3.0–4.0 mg∙L−1) and low indole-3-butyric acid (0.01–0.03 mg∙L−1) concentrations. Rooting was obtained in a medium without any growth regulators. Microscopic analysis showed a direct connection between the vascular elements of adventitious roots and stem of plantlet. Chromosome number in root apices of ash plantlets and ash trees grown under natural conditions was 2n = 46. Key words: chromosome number, Fraxinus excelsior L., in vitro plants, micropropagation, stem cuttings.

scholarly journals In vitro establishment and proliferation of red currant cultivars

2012 ◽  
Vol 39 (No. 1) ◽  
pp. 21-25 ◽  
Author(s):  
J. Sedlák ◽  
F. Paprštein
Keyword(s):  
Woody Plant ◽  
Adventitious Bud ◽  
Mineral Salts ◽  
The Czech Republic ◽  
Shoot Number ◽  
Adventitious Bud Formation ◽  
Woody Plant Medium ◽  
In Vitro Establishment ◽  
Red Currant

The goal of this study was to investigate in vitro multiplication protocols for use with red currant cultivars grown in the Czech Republic. Cultivars Detvan, Vitan and Rotte Höllandische were successfully established in vitro using mercuric chloride in a concentration of 0.15% as a sterilization solution. The overall rate of contamination was 25.7%. Two proliferation media Murashige and Skoog medium (MS) and McCown woody plant medium (WPM) containing 1 or 2 mg/l of 6-benzylaminopurine (BAP) were tested. Initial explants produced new plants in the form of rosettes. Rosettes arose from the base of the initial explants in the form of adventitious bud formation. The shoot number was relatively low and varied between 1.0 and 2.1. Generally, the highest number was obtained for cultivar Rotte Holländische that produced 2.1 ± 0.1 new rosettes on MS medium containing lower concentration 1 mg/l BAP. In contrary, Vitan cv. had significantly lower shoot number ranging from 1.0 to 1.3. WPM medium with a lower concentration of mineral salts proved to be unsuitable for the multiplication of tested cultivars.

Manipulation of desiccation-sensitive axes of wampee (Clausena lansium) to facilitate increased dehydration tolerance

2000 ◽  
Vol 10 (3) ◽  
pp. 397-400
Author(s):  
J.R. Fu ◽  
X.M. Huang ◽  
S.Q. Songa
Keyword(s):  
Butyric Acid ◽  
Water Loss ◽  
Adventitious Roots ◽  
Woody Plant ◽  
Dehydration Tolerance ◽  
Woody Plant Medium ◽  
Napthaleneacetic Acid ◽  
Pre Treatment

AbstractThe plumules of newly-excised wampee embryos, which are more sensitive to dehydration than the roots, became more resistant to water loss when axes were allowed to sprout on woody plant medium [WPM; McCown and Lloyd (1981) Hortscience16, 453] before being dried. Pre-treatment of sprouting axes (seedlings) with sucrose incorporated in the WPM enhanced survival. Although the roots withered following further dehydration of seedlings cultured on WPM containing 60% sucrose, excised plumules were capable of generating adventitious roots when a combination of 10 mM α-napthaleneacetic acid and 10 mM indole-3-butyric acid was used during subsequent in vitro incubation.

scholarly journals Micropropagation of sea buckthorn (Hippophae rhamnoides L.)

1995 ◽  
Vol 4 (5-6) ◽  
pp. 503-512
Author(s):  
Yingmou Yao
Keyword(s):  
Growth Regulator ◽  
Woody Plant ◽  
Average Rate ◽  
Root Nodules ◽  
Optimal Concentration ◽  
Sea Buckthorn ◽  
Adventitious Buds ◽  
Woody Plant Medium ◽  
Different Origins

A protocol for micropropagation of sea buckthorn was developed starting with shoot tips or meristems from plants up to 18 years old. Among the different media used, the best medium for both initiation and multiplication was the woody plant medium (WPM). 6-Benzylaminopurine (BAP) was the most suitable growth regulator with an optimal concentration of 0.10-0.25 mg/l for initiation and 0.4-1.0 mg/l for multiplication. On WPM medium with BAP, the average rate of multiplication in Erlenmeyer flasks was 3.3-4.0 shoots per explant per month and in test tubes 2.0-3.0 shoots. Moreover, most explants produced several to tens of adventitious buds which grew into shoots. Explants rooted spontaneously in the multiplication medium at a frequency of about 33%. With this method, explants of different origins have been successfully propagated in vitro; and rooted young plants which had developed root nodules were produced both in the greenhouse and in the field.

scholarly journals IN VITRO FLOWERING OF MINIATURE ROSE CULTIVARS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 694e-694
Author(s):  
Michael Kane ◽  
Nancy L. Philman ◽  
Francis J. Marousky
Keyword(s):  
Woody Plant ◽  
Cut Flowers ◽  
Mineral Salts ◽  
Lateral Buds ◽  
Woody Plant Medium ◽  
Causative Agents ◽  
Ascorbic Acids ◽  
Indole 3 Acetic Acid ◽  
Stem Segments

Premature deterioration and/or wilting of cut flowers such as roses (“bent neck”) has been attributed to vascular blockage within the cut stem. Vascular blockage has been attributed to both the proliferation of bacteria in the cut flower water and/or to products exuded by the stem. Separation of these causative agents is prevented by the inability to obtain intact microbe-free flowers. With the objective to produce microbe-free flowers, 36 miniature rose cultivars were screened for their capacity to flower in vitro. Stem segments containing single lateral buds were surface sterilized in 1.05% (v/v) sodium hypochlorite and rinsed three times in sterile distilled deionized water. Buds were established on medium consisting of Murashige and Skoog mineral salts, Woody Plant Medium organics, 3.0% (w/v) sucrose, 0.5 mg/liter benzyladenine, 0.1 mg/liter indole-3-acetic acid, and 50 mg/liter each citric and ascorbic acids. Medium was solidified with 1.5 g/liter gelrite and 4 g/liter TC® agar. Of the 36 cultivars screened, eight (22%) grew poorly in vitro. Of the 28 responsive cultivars, 14 (50%) produced flower buds in vitro However, only six cultivars produced open flowers in vitro.

scholarly journals 588 PB 129 IN VITRO MULTIPLICATION OF STEWARTIA MALACODENDRON L., AN ENDANGERED WOODY SPECIES

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 516b-516
Author(s):  
Murdock Ray Gillis ◽  
Michael E. Kane
Keyword(s):  
Woody Plant ◽  
Unit Cost ◽  
Woody Species ◽  
Shoot Multiplication ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Deciduous Tree ◽  
Landscape Plant ◽  
Woody Plant Medium

Stewartia malacodendron L. (silky camellia), a small deciduous tree bearing showy flowers, has potential as a landscape plant. Propagation problems, limited availability and consequent high unit cost have slowed its acceptance as a landscape plant. Given its potential value as a landscape plant, studies were initiated to define a micropropagation protocol. Surface sterilized shoot tips and nodal explants from two-year-old container grown seedlings were established on Woody Plant Medium supplemented with 4.44 μM benzyladenine (BA) and solidified with 0.8% TC® Agar. Sustained growth of subcultured shoot tips and nodal segments required the addition of 8-15 μM gibberellic acid (GA3) to the medium. Regenerated shoots were 3 - 5 cm long, unbranched and typically consisted of three subdivisions. Effects of cytokinin type (BA, 2iP or kinetin) and concentration (0-25 μM) with factorial combinations of GA3 (0-30 μM) on shoot multiplication, elongation and diameter were determined after a 28 day culture period. Moderate GA, levels (10 & 20 μM) in combination with 2.5 μM BA yielded the highest quality microcuttings.

scholarly journals The in vitro shoot regeneration of Ehretia asperula Zol. and Mor.

2019 ◽  
Vol 2 (6) ◽  
pp. 75-83
Author(s):  
Le Thi Thuy Tien
Keyword(s):  
Woody Plant ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Shoot Initiation ◽  
In Vitro Shoots ◽  
Woody Plant Medium ◽  
Number Of Leaves ◽  
Growth Experiments ◽  
In Vitro Shoot Regeneration

Xa den young branches in the orchard were sterilized and used as explants for shoot initiation and growth experiments. The shoot induction was carried out with BA (benzyl adenine) or TDZ (thidiazuron). New shoots sprouted after one week of culture and the highest shoots (2.02 cm) were on MS medium (Murashige and Skoog medium) with BA 0.6 mg/L after 3 weeks. Furthermore, the number of leaves per shoot was also higher than other treatments (8.31 leaves per shoot). In vitro shoots were used in other experiments to investigate the effects of explants, biotin concentrations, minerals, type and concentration of cytokinins on the formation and elongation of shoots and clusters. When 5 mg/L biotin was added to MS medium, shoots grew better. The MS medium appeared to be most suitable for the initiation and elongating of shoots, followed by WPM (woody plant medium) and SH medium (Schenk and Hildebrandt medium), while B5 medium (Gamborg B5 medium) was the least effective. The spouting from three-week-old explants was earlier than others (4 and 5 weeks of age), which in turn affected on the shoot elongating. BA 1.5 mg/L was suitable to induce shoot clusters (3.91 shoots per explant) after 4 weeks.

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