Partitioning of Cephalexin in Ionic Liquid Aqueous Two-Phase System Composed of 1-Butyl-3-Methylimidazolium Tetrafluoroborate andZnSO4

Ionic liquid aqueous two-phase system (ILATPS) was applied in the extraction and separation of hydrosoluble antibiotics. The partitioning behavior of cephalexin (CEX) in 1-butyl-3-methylimidazolium tetrafluoroborate ([Bmim]BF4)-ZnSO4aqueous two-phase system was studied by the partitioning parameter of the extraction efficiency. The effect of the volume of [Bmim]BF4, the concentration of ZnSO4, temperature, pH, and the volume of ZnSO4solution was discussed concretely. When the volume of [Bmim]BF4was 2 mL and the concentration of ZnSO4was 35%, the extraction efficiency of CEX could reach 92.64% with pH unadjusted. The effect of the volume of [Bmim]BF4on the extraction efficiency was higher than that of the concentration of ZnSO4. The temperature influenced not only the formation of aqueous two-phase system but also the extraction efficiency of CEX. The target was found to be preferentially extracted to the [Bmim]BF4-rich phase at the pH below 4.3. The partition of CEX to the top phase was enhanced by increasing the volume of [Bmim]BF4, the concentration of ZnSO4, and temperature; however, the partition of CEX to the top phase increased by decreasing the pH.

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Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

Journal of Chemistry ◽

10.1155/2013/938154 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 5

Author(s):

Zhiwen Bai ◽

Yanhong Chao ◽

Meiling Zhang ◽

Changri Han ◽

Wenshuai Zhu ◽

...

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Extraction Efficiency ◽

Phase System ◽

Optimum Conditions ◽

Two Phase ◽

Rich Phase ◽

Aqueous Two Phase Systems ◽

Phase Systems ◽

Partitioning Behavior

This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.

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Partitioning Behavior of Penicillin G in Aqueous Two Phase System Based on Ionic Liquids

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.864-867.324 ◽

2013 ◽

Vol 864-867 ◽

pp. 324-327

Author(s):

Bin Jiang ◽

Zhi Biao Feng ◽

Chun Hong Liu ◽

Ying Cao Xu ◽

Dong Mei Li ◽

...

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Extraction Yield ◽

Penicillin G ◽

Phase System ◽

Two Phase ◽

Aqueous Two Phase System ◽

Partitioning Behavior

An aqueous two-phase system (ATPS) was presented with hydrophilic ionic liquid 1-Butyl-3-methylimid-azolium tetrafluoroborate ([BmiBF4) and salts for the extraction of penicillin G in this paper. The phase forming abilities of different salts were investigated to choose a suitable salt.The partitioning behavior of penicillin G in the ATPS was investigated.Concentrations of (NH4)2SO4, penicillin G, and [BmiBF4 were evaluated to determine their effects on the extraction yield of penicillin G. It was found that extraction yield strongly depended on the concentration of [BmiBF4, penicillin and (NH4)2SO4. A high extraction yield around 93% was achieved with the following parameters: (NH4)2SO4 20% (wt%), penicillin 40000 ~ 45000 U/mL, [BmiBF4 20% (wt%).

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