scholarly journals Activation of MITF by Argan Oil Leads to the Inhibition of the Tyrosinase and Dopachrome Tautomerase Expressions in B16 Murine Melanoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Myra O. Villareal ◽  
Sayuri Kume ◽  
Thouria Bourhim ◽  
Fatima Zahra Bakhtaoui ◽  
Kenichi Kashiwagi ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Scientific Basis ◽  
Melanin Biosynthesis ◽  
Dopachrome Tautomerase ◽  
Murine Melanoma ◽  
Argan Oil ◽  
Dose Dependent Decrease ◽  
Murine Melanoma Cells

Argan (Argania spinosaL.) oil has been used for centuries in Morocco as cosmetic oil to maintain a fair complexion and to cure skin pimples and chicken pox pustules scars. Although it is popular, the scientific basis for its effect on the skin has not yet been established. Here, the melanogenesis regulatory effect of argan oil was evaluated using B16 murine melanoma cells. Results of melanin assay using B16 cells treated with different concentrations of argan oil showed a dose-dependent decrease in melanin content. Western blot results showed that the expression levels of tyrosinase (TYR), tyrosinase-related protein 1 (TRP1), and dopachrome tautomerase (DCT) proteins were decreased. In addition, there was an increase in the activation of MITF and ERK1/2. Real-time PCR results revealed a downregulation ofTyr,Trp1,Dct, andMitfmRNA expressions. Argan oil treatment causes MITF phosphorylation which subsequently inhibited the transcription of melanogenic enzymes, TYR and DCT. The inhibitory effect of argan oil on melanin biosynthesis may be attributed to tocopherols as well as the synergistic effect of its components. The results of this study provide the scientific basis for the traditionally established benefits of argan oil and present its therapeutic potential against hyperpigmentation disorders.

scholarly journals Tangeretin Triggers Melanogenesis through the Activation of Melanogenic Signaling Proteins and Sustained Extracellular Signal-Regulated Kinase in B16/F10 Murine Melanoma Cells

2015 ◽  
Vol 10 (3) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Hoon Seok Yoon ◽  
Hee-Chul Ko ◽  
Sang Suk Kim ◽  
Kyung Jin Park ◽  
Hyun Joo An ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Melanoma Cells ◽  
Response To Treatment ◽  
Signaling Proteins ◽  
Dependent Manner ◽  
Melanin Content ◽  
Extracellular Signal Regulated Kinase ◽  
Murine Melanoma ◽  
Extracellular Signal ◽  
Murine Melanoma Cells

In order to test the effectiveness of tangeretin at ameliorating melanoma and melanoma-associated depigmentation, western blotting was used to assess the melanin content of treated melanoma cells. Tangeretin, a 4′,5,6,7,8-pentamethoxyflavone, was found to trigger intracellular melanin production in a concentration-dependent manner in B16/F10 murine melanoma cells. Melanin content increased 1.74-fold in response to treatment with 25 μM of tangeretin, compared to that in non-treated cells. Examination of melanogenic protein expression showed that tyrosinase, tyrosinase-related protein (TRP)-1, and extracellular signal-regulated kinase (ERK) 1/2 levels increased in a dose-dependent manner. Furthermore, the expression of cyclic adenosine monophosphate response element binding protein (CREB) and microphthalmia transcription factor (MITF) was increased by tangeretin in 1 h and 4 h, respectively. Tangeretin-upregulated melanogenesis was suppressed by ERK 1/2 inhibitor and not by ERK1 inhibitor. These results suggest that tangeretin has therapeutic potential for melanoma and melanoma-associated depigmentation because it can induce hyperpigmentation through the activation of melanogenic signaling proteins and initiation of sustained ERK2 expression.

scholarly journals Synthesis of Resorcinol Derivatives and their Effects on Melanin Production

Cosmetics ◽  
2020 ◽  
Vol 7 (3) ◽  
pp. 55
Author(s):  
Yoshihiro Tokudome ◽  
Tsuyoshi Hoshi ◽  
Sayaka Mori ◽  
Ichiro Hijikuro
Keyword(s):  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
Melanin Production ◽  
Skin Whitening ◽  
Alkyl Chains ◽  
Murine Melanoma ◽  
Resorcinol Derivative ◽  
Melanin Contents ◽  
Murine Melanoma Cells

Several resorcinol derivatives were synthesized and their effects on the survival rate of B16 murine melanoma cells, melanin production, and tyrosinase activity were investigated with an aim to evaluate their skin whitening effect. Twelve resorcinol derivatives were synthesized by esterification with three functional groups (L-ascorb-6-yl, ethyl, and glyceryl) linked via four alkyl chains of varying lengths (n = 2–5) at the 4-position. The structures of the 12 resorcinol derivatives were confirmed by Nuclear Magnetic Resonance (NMR). The derivatives were added to B16 murine melanoma cells and the melanin contents in the cells and culture medium were measured. To measure the tyrosinase activity, the substrate L-DOPA was added to a mushroom-derived tyrosinase solution, and the inhibition of the tyrosinase activity was determined. At 10 µM, the resorcinol derivatives did not affect the survival of the B16 murine melanoma cells, but the melanin content was reduced. At 1 µM, the derivatives significantly inhibited the tyrosinase activity in the mushroom-derived tyrosinase solution. A plot of the inhibitory effect on melanin production against the cLogP value for each resorcinol derivative indicated that the highest inhibition occurred at a cLogP value of approximately 2. Therefore, these resorcinol derivatives are expected to serve as effective skin whitening agents.

scholarly journals Antaroide, a Novel Natural Nine-Membered Macrolide, Inhibits Melanin Biosynthesis in B16F10 Murine Melanoma Cells

2021 ◽  
Vol 29 (1) ◽  
pp. 98-103
Author(s):  
Min-Ji Ryu ◽  
Eun-Ki Baek ◽  
Soyeon Kim ◽  
Chi Nam Seong ◽  
Inho Yang ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Melanin Biosynthesis ◽  
Murine Melanoma ◽  
Murine Melanoma Cells
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